专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5965404A Method for introducing nucleic acids into higher eukaryotic cells 失效
标题翻译：将核酸引入高等真核细胞的方法
公开(公告)号：US5965404A
公开(公告)日：1999-10-12
申请号：US750181
申请日：1997-03-06
申请人： Michael Buschle , Ernst Wagner , Wolfgang Zauner
发明人： Michael Buschle , Ernst Wagner , Wolfgang Zauner
IPC分类号： C12N15/09 , A61K35/12 , A61K47/48 , A61K48/00 , A61P35/00 , C12N5/10 , C12N15/87 , C12N15/63 , C12N5/16
CPC分类号： C12N15/87
摘要： Process and medium for the transfection of higher eukaryotic cells with DNA/polycation complexes, wherein a medium is used which contains ethyleneglycol and/or glycerol. The medium may also contain a substance which prevents the acidification of the endosomes, and/or a lower alcohol such as ethanol. The process is particularly suitable for the transfection of primary cells such as fibroblasts. Stably transformed cells can be obtained thereby, e.g. tumour cells for use as tumour vaccines.
摘要翻译： PCT No.PCT / EP95 / 01969。 371日期1997年3月6日 102（e）1997年3月6日PCT PCT 1995年5月24日PCT公布。公开号WO95 / 33061 日期1995年12月7日用DNA /聚阳离子配合物转染高等真核细胞的方法和培养基，其中使用含有乙二醇和/或甘油的培养基。介质还可以含有防止内体的酸化的物质和/或低级醇如乙醇。该方法特别适用于原代细胞如成纤维细胞的转染。可以获得稳定转化的细胞，例如，肿瘤细胞用作肿瘤疫苗。

2. 发明授权

US5521291A Conjugates for introducing nucleic acid into higher eucaryotic cells 失效
标题翻译：用于将核酸引入高级真核细胞的缀合物
公开(公告)号：US5521291A
公开(公告)日：1996-05-28
申请号：US166899
申请日：1993-12-15
申请人： David T. Curiel , Ping-chuan Hu , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
发明人： David T. Curiel , Ping-chuan Hu , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
IPC分类号： A61K47/48 , C07K16/08 , C12N15/87 , A61K39/42 , C07H19/00 , C12N7/00
CPC分类号： C12N15/87 , A61K47/48692 , A61K47/48776 , C07K16/081
摘要： Conjugates in which a virus is bound via an antibody to a substance having an affinity for nucleic acid, for transporting gene constructs into higher eucaryotic cells. Complexes of the conjugates and nucleic acid are internalized in the cell, whilst the virus as part of the complex brings about the internalization and the release of the contents of the endosomes, in which the complexes are located after entering the cell. Pharmaceutical preparations in which the nucleic acid is a therapeutically active gene construct, particularly for use in gene therapy.
摘要翻译：通过抗体将病毒结合到对核酸具有亲和性的物质，用于将基因构建体转运到较高真核细胞中的缀合物。缀合物和核酸的复合物被内化在细胞中，而作为复合物的一部分的病毒导致内含体的内化和释放，其中复合物在进入细胞后位于其中。其中核酸是治疗活性基因构建体，特别是用于基因治疗的药物制剂。

3. 发明申请

US20140371292A1 Method for the controlled intracellular delivery of nucleic acids 审中-公开
标题翻译：核酸控制细胞内递送的方法
公开(公告)号：US20140371292A1
公开(公告)日：2014-12-18
申请号：US14125914
申请日：2012-08-10
申请人： Ahmed Besheer , Matthaus Noga , Gerhard Winter , Ernst Wagner , Daniel Edinger
发明人： Ahmed Besheer , Matthaus Noga , Gerhard Winter , Ernst Wagner , Daniel Edinger
IPC分类号： A61K47/36 , A61K31/713
CPC分类号： A61K47/36 , A61K31/713 , A61K47/59 , A61K47/61
摘要： The present invention relates to a method for the controlled intracellular delivery of nucleic acid molecules into one or more target cells, in particular tumor cells, the method comprising: providing a polymeric complex formed between one or more nucleic acid molecules to be delivered and one or more cationic carrier molecules, wherein at least a part of the one or more carrier molecules in the polymeric complex are covalently attached to hydroxyalkyl starch, and wherein the hydroxyalkyl starch is shielding the polymeric complex; allowing the shielded polymeric complex to get into contact with the one or more target cells; deshielding the polymeric complex by removing the hydroxyalkyl starch; and allowing the deshielded polymeric complex to internalize into the one or more target cells. Removal of the hydroxyalkyl starch can be accomplished enzymatically by exposing the polymeric complex to amylase. The invention also concerns the use of such method for the prevention and/or treatment of a condition selected from the group consisting of cancer, immune diseases, cardiovascular diseases, neuronal diseases, infections, and inflammatory diseases.
摘要翻译：本发明涉及将核酸分子受控的细胞内递送到一个或多个靶细胞，特别是肿瘤细胞中的方法，所述方法包括：提供在待递送的一个或多个核酸分子之间形成的聚合物复合物，更多的阳离子载体分子，其中聚合物络合物中的一个或多个载体分子的至少一部分共价连接到羟烷基淀粉上，并且其中羟烷基淀粉屏蔽聚合复合物; 允许屏蔽聚合物复合物与一个或多个靶细胞接触; 通过除去羟烷基淀粉去除聚合物复合物; 并且允许未屏蔽的聚合物络合物内化到一个或多个靶细胞中。可以通过将聚合物复合物暴露于淀粉酶来酶促去除羟烷基淀粉。本发明还涉及使用这种方法来预防和/或治疗选自癌症，免疫疾病，心血管疾病，神经元疾病，感染和炎性疾病的病症。

4. 发明申请

US20080092633A1 METHOD FOR DETECTING THE HERMETIC SEALING OF ENCLOSED AREAS 有权
标题翻译：检测封闭区域的封闭密封方法
公开(公告)号：US20080092633A1
公开(公告)日：2008-04-24
申请号：US11838390
申请日：2007-08-14
申请人： Ernst Wagner
发明人： Ernst Wagner
IPC分类号： G01M3/00
CPC分类号： A62C99/0018 , G01M3/226
摘要： This invention relates to a method by means of which it is possible, with highest possible precision and without any major expense, to determine the air tightness of an enclosed space, such space being particularly well suited to the use of the inert-gas fire extinguishing technology described above. Specifically, it introduces a method which, on demand and in effective yet easy-to-implement fashion, permits at any time the updated determination of the existing air tightness of the enclosed space without requiring a complex test series. To that effect, the first step according to the invention is to define a concentration gradient between the internal air atmosphere of an enclosed space and the ambient air atmosphere by setting the physical concentration of at least one constituent component of the space-internal air atmosphere, in particular of oxygen, at a value that differs from the physical concentration value of that minimum of one corresponding component in the ambient air atmosphere. This is followed by a determination of the concentration change rate in that, in the space-internal air atmosphere, the time-based change of the physical concentration of the minimum of one constituent component is measured. Finally, taking into account the previously determined concentration change rate, the air-tightness value of the enclosed space is calculated.
摘要翻译：本发明涉及一种方法，通过该方法可以以最高可能的精度和无任何主要的费用来确定封闭空间的气密性，这种空间特别适合于使用惰性气体灭火上述技术具体来说，它引入了一种方法，按需和有效但易于实施的方式，可随时更新确定封闭空间的现有气密性，而无需复杂的测试系列。为此，根据本发明的第一步骤是通过设定空间 - 内部空气气氛的至少一个构成成分的物理浓度来限定封闭空间的内部空气气氛与环境空气气氛之间的浓度梯度，特别是氧气，其值不同于环境空气气氛中的一个对应组分的最小值的物理浓度值。接着测定浓度变化率，即在空间 - 内部空气气氛中，测定一个构成成分的最小值的物理浓度的时间变化。最后，考虑到先前确定的浓度变化率，计算封闭空间的气密性值。

5. 发明授权

US5981273A Composition comprising an endosomolytic agent for introducing nucleic acid complexes into higher eucaryotic cells 失效
标题翻译：组合物，其包含用于将核酸复合物引入高级真核细胞的内体分解剂
公开(公告)号：US5981273A
公开(公告)日：1999-11-09
申请号：US450417
申请日：1995-05-25
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： A61K47/48 , A61K48/00 , C07K14/11 , C07K14/775 , C07K14/79 , C12N15/87 , C07H21/02 , C12N7/00
CPC分类号： A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/775 , C07K14/79 , C12N15/87 , C07K2319/00 , C12N2740/16022
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

6. 发明申请

US20070079973A1 Device for preventing and extinguishing fires 有权
标题翻译：防火灭火装置
公开(公告)号：US20070079973A1
公开(公告)日：2007-04-12
申请号：US10574733
申请日：2004-11-09
申请人： Ernst Wagner
发明人： Ernst Wagner
IPC分类号： A62C8/00 , A62C3/06 , A62C37/10
CPC分类号： A62C99/0018 , A62C35/15
摘要： A device for the prevention and extinguishing of fires is specified having the objective of providing an inert gas fire extinguishing system which is configured in accordance with the invention such that no breaches to ceilings or walls need be made when mounting the supply line system (4) during installation of the fire extinguishing system in a target area (1), and also having the further objective of dispensing with separate storage areas for the pressurized oxygen-displacing gas (3) used as extinguishing agent, wherein a buffer reservoir (2), the supply line system (4) and extinguishing nozzles (5) are arranged as a compact modular unit right within target area (1) itself.
摘要翻译：规定了用于防止和熄灭火灾的装置，其目的是提供一种根据本发明构造的惰性气体灭火系统，使得在安装供应管线系统（4）时不需要制造天花板或墙壁的缺陷，在将灭火系统安装在目标区域（1）中，并且还具有为用作灭火剂的加压置换气体（3）分配单独的存储区域的目的，其中缓冲储存器（2）供应线路系统（4）和灭火喷嘴（5）被设置成紧邻目标区域（1）内的紧凑模块单元。

7. 发明申请

US20050155407A1 Method and device for measuring oxygen content in a closed target space 有权
标题翻译：用于测量封闭目标空间中氧含量的方法和装置
公开(公告)号：US20050155407A1
公开(公告)日：2005-07-21
申请号：US10500437
申请日：2002-10-17
申请人： Ernst Wagner
发明人： Ernst Wagner
IPC分类号： A62C99/00 , G01N33/00 , G08B17/117 , G01N7/00
CPC分类号： G08B17/117 , A62C99/0018 , G01N33/0063
摘要： A method and apparatus for measuring the oxygen content in a closed target space, particularly for monitoring inertization levels in an inert gas device for fire prevention and/or fire extinguishing. Toward the aim of proposing a method for measuring the oxygen content in a target space with which an effective, certain, and representative determination of the oxygen concentration can be achieved for an optimally small expenditure in instrumentation and signal processing, the method provides that air samples are drawn from the target space and the oxygen concentration of the air samples is determined. An apparatus is equipped with a suction pipe system for sucking the air sample from the target space through various holes so that it can carry out the method.
摘要翻译：一种用于测量封闭目标空间中的氧含量的方法和装置，特别是用于监测用于防火和/或灭火的惰性气体装置中的惰性化水平。为了提出一种用于测量目标空间中的氧含量的方法的目的，通过该方法可以实现有效，确定和代表性的氧浓度确定，以便在仪器和信号处理中的最佳小的支出，该方法提供空气样品从目标空间中抽出，并确定空气样本的氧气浓度。一种装置配备有用于从目标空间通过各种孔吸入空气样本的吸管系统，从而可以执行该方法。

8. 发明申请

US20050004362A1 Method of reducing the organic carbon content of production waste water in the course of the preparation of concentrated solutions of anionic organic compounds 失效
标题翻译：在制备阴离子有机化合物浓缩溶液的过程中，减少生产废水中有机碳含量的方法
公开(公告)号：US20050004362A1
公开(公告)日：2005-01-06
申请号：US10494595
申请日：2002-10-31
申请人： Kurt Huber , Rolf Lucas , Jacek Malisz , Ernst Wagner
发明人： Kurt Huber , Rolf Lucas , Jacek Malisz , Ernst Wagner
IPC分类号： C02F1/44 , C09B67/24 , C09B67/26 , C09B67/54 , C07
CPC分类号： C09B67/0096 , C02F1/442 , C02F1/444 , C02F2101/30 , C02F2101/308 , C02F2103/28 , C09B67/0073
摘要： The present invention relates to a method of reducing the total organic carbon (TOC) content of waste water in the course of the preparation of concentrated solutions or suspensions of anionic organic compounds, which method comprises increasing the concentration of an aqueous solution or suspension of an anionic organic compound in the form of its free acid or its alkali metal salt, having a salt content of less than 5% of extraneous salt by weight based on the total solution or suspension, by microfiltration, ultrafiltration and/or nanofiltration, a) the membrane pore size being so selected that compounds having molecular weights in the range from 300 to 1000 Daltons or higher arc retained, and b) the content of anionic compound in the concentrate being so adjusted to from 10 to 50% by weight that the total organic carbon (TOC) content of the permeate is less than 0.5% by weight, based on the total permeate, and c) optionally, after increasing the concentration of a suspension, dissolving the anionic organic compound by adding a suitable base. The invention relates also to the solutions and suspensions prepared by that means and to the use of such solutions or suspensions in dyeing or optically whitening paper or textile materials.
摘要翻译：本发明涉及一种在制备阴离子有机化合物的浓缩溶液或悬浮液的过程中降低废水总有机碳（TOC）含量的方法，该方法包括增加阴离子有机化合物的水溶液或悬浮液的浓度其游离酸或其碱金属盐形式的阴离子有机化合物，其盐含量基于总溶液或悬浮液，通过微量过滤，超滤和/或纳滤，重量小于外来盐的5％，a）选择膜孔径，使得分子量在300至1000道尔顿或更高的范围内的化合物保持电弧，并且b）将浓缩物中的阴离子化合物的含量调节至10至50重量％，使总有机基于总渗透物，渗透物的碳（TOC）含量小于0.5重量％，c）任选地，在增加悬浮液的浓度后，通过加入合适的碱来洗涤阴离子有机化合物。本发明还涉及通过这种方法制备的溶液和悬浮液以及这种溶液或悬浮液在染色或光学增白纸或纺织材料中的用途。

9. 发明授权

US5693509A Adenovirus for delivering foreign DNA into higher eukaryotic cells 失效
标题翻译：腺病毒用于将外源DNA递送入高等真核细胞
公开(公告)号：US5693509A
公开(公告)日：1997-12-02
申请号：US530181
申请日：1995-09-29
申请人： Matthew Cotten , Ernst Wagner
发明人： Matthew Cotten , Ernst Wagner
IPC分类号： C12N15/09 , A61K35/14 , A61K35/76 , A61K48/00 , C12N7/00 , C12N7/01 , C12N7/04 , C12N15/861 , C12N15/87 , C12N5/00 , C07K3/08
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N15/87 , C12N2710/10323 , C12N2710/10343 , C12N2710/10345 , C12N2710/10362 , C12N2810/80
摘要： Viruses or cells are targeted for selective internalization into a target in vive. A molecule Specific for a receptor on the surface of the target cell is introduced onto the surface of the virus or cell. The modified virus or cell binds the receptor in vive and is internalized by the target cell. The method provides vectors for selective delivery of nucleic acids to specific cell types in vivo and a means to alter the tropism of an infectious agent.
摘要翻译： PCT No.PCT / EP94 / 01065 Sec。 371 1995年9月29日第 102（e）1995年9月29日PCT PCT 1994年4月6日PCT公布。第WO94 / 24299号公报日期1994年10月27日病毒或细胞的目标是选择性地内化到一个目标。将靶细胞表面上的受体特异性的分子引入病毒或细胞的表面。修饰的病毒或细胞在vive中结合受体，并被靶细胞内化。该方法提供用于在体内选择性递送核酸到特定细胞类型的载体，以及改变感染因子的向性的方法。

10. 发明申请

US20080112916A1 CHEMICALLY MODIFIED POLYCATION POLYMER FOR siRNA DELIVERY 审中-公开
标题翻译：用于siRNA递送的化学改性聚合物
公开(公告)号：US20080112916A1
公开(公告)日：2008-05-15
申请号：US11777539
申请日：2007-07-13
申请人： Ernst Wagner , Julia Klockner , Peter Tarcha , Thomas Merdan
发明人： Ernst Wagner , Julia Klockner , Peter Tarcha , Thomas Merdan
IPC分类号： A61K48/00 , C08G63/48
CPC分类号： C08G73/0226 , A61K47/59 , A61K47/60 , C12N15/111 , C12N15/87 , C12N2310/14 , C12N2320/32
摘要： The present invention provides a unique non-viral carrier for nucleic acid delivery in vitro and in vivo, and methods of using thereof.
摘要翻译：本发明提供用于体外和体内核酸递送的独特的非病毒载体及其使用方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式