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1. 发明授权

US5521291A Conjugates for introducing nucleic acid into higher eucaryotic cells 失效
标题翻译：用于将核酸引入高级真核细胞的缀合物
公开(公告)号：US5521291A
公开(公告)日：1996-05-28
申请号：US166899
申请日：1993-12-15
申请人： David T. Curiel , Ping-chuan Hu , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
发明人： David T. Curiel , Ping-chuan Hu , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
IPC分类号： A61K47/48 , C07K16/08 , C12N15/87 , A61K39/42 , C07H19/00 , C12N7/00
CPC分类号： C12N15/87 , A61K47/48692 , A61K47/48776 , C07K16/081
摘要： Conjugates in which a virus is bound via an antibody to a substance having an affinity for nucleic acid, for transporting gene constructs into higher eucaryotic cells. Complexes of the conjugates and nucleic acid are internalized in the cell, whilst the virus as part of the complex brings about the internalization and the release of the contents of the endosomes, in which the complexes are located after entering the cell. Pharmaceutical preparations in which the nucleic acid is a therapeutically active gene construct, particularly for use in gene therapy.
摘要翻译：通过抗体将病毒结合到对核酸具有亲和性的物质，用于将基因构建体转运到较高真核细胞中的缀合物。缀合物和核酸的复合物被内化在细胞中，而作为复合物的一部分的病毒导致内含体的内化和释放，其中复合物在进入细胞后位于其中。其中核酸是治疗活性基因构建体，特别是用于基因治疗的药物制剂。

2. 发明授权

US06274322B1 Composition for introducing nucleic acid complexes into higher eucaryotic cells 有权
标题翻译：用于将核酸复合物引入高级真核细胞的组合物
公开(公告)号：US06274322B1
公开(公告)日：2001-08-14
申请号：US09465646
申请日：1999-12-16
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： C12Q168
CPC分类号： C12N15/87 , A61K38/00 , A61K47/50 , A61K47/6901 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C07K2317/77 , C07K2319/00 , C12N7/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

3. 发明授权

US5922859A Complexes containing nucleic acid which can be taken-up by endocytosis into higher eukaryotic cells 失效
标题翻译：含有核酸的复合物，其可通过内吞作用摄入高等真核细胞
公开(公告)号：US5922859A
公开(公告)日：1999-07-13
申请号：US98265
申请日：1993-08-05
申请人： Max L. Birnstiel , Matthew Cotten , Ernst Wagner
发明人： Max L. Birnstiel , Matthew Cotten , Ernst Wagner
IPC分类号： A61K48/00 , C12N15/87 , C07H21/04
CPC分类号： A61K48/00 , C12N15/87
摘要： Complexes between internalizing factor-bonding factor conjugates and nucleic acid which can be taken up into higher eukaryotic cells by endocytosis containing, in non-covalently bound form, one or more substances having an affinity for nucleic acid, which are capable of increasing the efficiency of absorption of the complexes into the cells. The non-covalently bound substance having an affinity for nucleic acid may optionally be identical to the bonding factor, preferably a polycationic substance. The internalizing factor is preferably transferrin. Processes for preparing the complexes and processes for introducing nucleic acid into higher eukaryotic cells. Pharmaceutical preparations containing complexes with a therapeutically active nucleic acid.
摘要翻译： PCT No.PCT / EP92 / 00217 Sec。 371日期：1993年8月5日 102（e）日期1993年8月5日PCT提交1992年2月1日PCT公布。出版物WO92 / 13570 日期1992年8月20日内部化因子结合因子偶联物和核酸之间的复合物，其可以通过内吞作用吸收到较高真核细胞中，所述胞吞作用以非共价结合形式含有一种或多种对核酸具有亲和力的物质，其具有能力提高复合物吸收细胞的效率。对核酸具有亲和力的非共价结合物质可任选地与结合因子相同，优选聚阳离子物质。内在因子优选为转铁蛋白。用于制备复合物的方法和将核酸引入更高真核细胞的方法。含有与治疗活性核酸复合物的药物制剂。

4. 发明授权

US5354844A Protein-polycation conjugates 失效
标题翻译：蛋白质聚阳离子共轭物
公开(公告)号：US5354844A
公开(公告)日：1994-10-11
申请号：US492460
申请日：1990-03-09
申请人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
发明人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
IPC分类号： C12N15/09 , A61K38/00 , A61K47/48 , A61P31/12 , A61P35/00 , C07K17/08 , C07K19/00 , C12N15/64 , C12N15/87 , C12Q1/68 , C07K3/08 , C07K15/06 , C12N15/90
CPC分类号： A61K47/48315 , C12N15/64 , C12N15/87
摘要： The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed with the polyanionic nucleic acid. This complex is bound to the transfertin receptor, which is highly regulated in growing cells, and absorbed into the cell. Suitable nucleic acids include those which inhibit specific genes or the RNA function, such as antisense oligonucleotides or ribozymes or the genes coding for them. The invention further relates to a process for introducing nucleic acids into the cells, transferrin-polycation/nucleic acid complexes and pharmaceutical preparations containing them.
摘要翻译：本发明涉及用于将核酸输送到细胞中的系统，其通过受体介导的内吞作用来实现。使用转铁蛋白 - 聚阳离子缀合物，可以用聚阴离子核酸形成络合物。该复合物与转铁蛋白受体结合，转铁蛋白受体在生长细胞中被高度调节并被吸收进入细胞。合适的核酸包括抑制特异性基因或RNA功能的核酸，如反义寡核苷酸或核酶或编码它们的基因。本发明还涉及将核酸引入细胞，转铁蛋白 - 聚阳离子/核酸复合物和含有它们的药物制剂的方法。

5. 发明授权

US06830923B1 Genetics units for inhibiting the function of RNA 失效
标题翻译：用于抑制RNA功能的遗传单位
公开(公告)号：US06830923B1
公开(公告)日：2004-12-14
申请号：US07947982
申请日：1992-09-21
申请人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Harald Kandolf
发明人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Harald Kandolf
IPC分类号： C12N1500
CPC分类号： C12N15/113 , A61K38/00 , C07K2319/00 , C12N2310/111 , C12N2310/121
摘要： The invention relates to a genetic unit, optionally present as a multiple-copy, for inhibiting RNA. The unit contains the transcription units necessary for transcription by polymerase III and a DNA coding for inhibiting RNA, which is arranged within the unit in such a way that the transcribed RNA is part of the polymerase III transcript. Using these units it is possible to achieve increased stability of the inhibiting RNA, which may occur in the form of ribozymes or antisense-RNAs, whilst maintaining an undiminished activity. The invention further relates to a process for introducing the genetic units into the cell, the use of these units and pharmaceutical preparations containing them.
摘要翻译：本发明涉及任选以多拷贝的形式存在用于抑制RNA的遗传单位。该单元包含通过聚合酶III转录所需的转录单位和编码抑制RNA的DNA，其以这样的方式排列在单位内，使得转录的RNA是聚合酶III转录物的一部分。使用这些单元，可以实现抑制性RNA的增加的稳定性，其可以以核酶或反义RNA的形式存在，同时保持未减弱的活性。本发明还涉及将遗传单位引入细胞的方法，使用这些单位和含有它们的药物制剂。

6. 发明授权

US6077663A Composition for introducing nucleic acid complexes into higher eucaryotic cells 失效
标题翻译：用于将核酸复合物引入高级真核细胞的组合物
公开(公告)号：US6077663A
公开(公告)日：2000-06-20
申请号：US449754
申请日：1995-05-25
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G.M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G.M. Schmidt
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K38/21 , A61K38/36 , A61K39/44 , A61K47/48 , A61K48/00 , A61P31/12 , A61P35/00 , C07K14/005 , C07K14/075 , C07K14/095 , C07K14/11 , C07K14/15 , C07K14/16 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/28 , C12N5/10 , C12N7/04 , C12N15/34 , C12N15/41 , C12N15/44 , C12N15/87 , G01N33/00 , C12Q1/68
CPC分类号： C12N15/87 , A61K47/48 , A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C12N7/00 , A61K38/00 , C07K2317/77 , C07K2319/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

7. 发明授权

US6022735A Composition for introducing nucleic acid complexes into higher eucaryotic cells 失效
公开(公告)号：US6022735A
公开(公告)日：2000-02-08
申请号：US449741
申请日：1995-05-25
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K38/21 , A61K38/36 , A61K39/44 , A61K47/48 , A61K48/00 , A61P31/12 , A61P35/00 , C07K14/005 , C07K14/075 , C07K14/095 , C07K14/11 , C07K14/15 , C07K14/16 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/28 , C12N5/10 , C12N7/04 , C12N15/34 , C12N15/41 , C12N15/44 , C12N15/87 , G01N33/00 , C12N15/63 , C07H21/04 , C12N15/00 , C12Q1/70
CPC分类号： C12N15/87 , A61K47/48 , A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C12N7/00 , A61K38/00 , C07K2317/77 , C07K2319/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

8. 发明授权

US5792645A Protein-polycation nucleic acid complexes and methods of use 失效
标题翻译：蛋白质聚阳离子核酸复合物及其使用方法
公开(公告)号：US5792645A
公开(公告)日：1998-08-11
申请号：US254888
申请日：1994-06-06
申请人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
发明人： Hartmut Beug , Max L. Birnstiel , Matthew Cotten , Ernst Wagner
IPC分类号： C12N15/09 , A61K38/00 , A61K47/48 , A61P31/12 , A61P35/00 , C07K17/08 , C07K19/00 , C12N15/64 , C12N15/87 , C12Q1/68 , C12N5/16 , C07K17/06
CPC分类号： A61K47/48315 , C12N15/64 , C12N15/87
摘要： The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed with the polyanionic nucleic acid. This complex is bound to the transferrin receptor, which is highly regulated in growing cells, and absorbed into the cell. Suitable nucleic acids include those which inhibit specific genes or the RNA function, such as antisense oligonucleotides or ribozymes or the genes coding for them. The invention further relates to a process for introducing nucleic acids into the cells, transferrin-polycation/nucleic acid complexes and pharmaceutical preparations containing them.
摘要翻译：本发明涉及用于将核酸输送到细胞中的系统，其通过受体介导的内吞作用来实现。使用转铁蛋白 - 聚阳离子缀合物，可以用聚阴离子核酸形成络合物。该复合物与转铁蛋白受体结合，转铁蛋白受体在生长细胞中被高度调节并被吸收进入细胞。合适的核酸包括抑制特异性基因或RNA功能的核酸，如反义寡核苷酸或核酶或编码它们的基因。本发明还涉及将核酸引入细胞，转铁蛋白 - 聚阳离子/核酸复合物和含有它们的药物制剂的方法。

9. 发明授权

US5547932A Composition for introducing nucleic acid complexes into higher eucaryotic cells 失效
公开(公告)号：US5547932A
公开(公告)日：1996-08-20
申请号：US948357
申请日：1992-09-23
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： C12N15/09 , A61K35/76 , A61K38/00 , A61K38/21 , A61K38/36 , A61K39/44 , A61K47/48 , A61K48/00 , A61P31/12 , A61P35/00 , C07K14/005 , C07K14/075 , C07K14/095 , C07K14/11 , C07K14/15 , C07K14/16 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/28 , C12N5/10 , C12N7/04 , C12N15/34 , C12N15/41 , C12N15/44 , C12N15/87 , G01N33/00 , C12Q1/70 , A01N63/00 , C07H21/04 , C12N15/00
CPC分类号： C12N15/87 , A61K47/48 , A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/56 , C07K14/775 , C07K14/79 , C07K16/00 , C07K16/2803 , C12N7/00 , A61K38/00 , C07K2317/77 , C07K2319/00 , C12N2710/10251 , C12N2710/10261 , C12N2710/10322 , C12N2710/10351 , C12N2710/10361 , C12N2710/10363 , C12N2740/11022 , C12N2740/15022 , C12N2740/16022 , C12N2740/16122 , C12N2760/16122 , C12N2770/32722 , C12N2770/32761 , Y10T436/143333
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.

10. 发明授权

US5981273A Composition comprising an endosomolytic agent for introducing nucleic acid complexes into higher eucaryotic cells 失效
标题翻译：组合物，其包含用于将核酸复合物引入高级真核细胞的内体分解剂
公开(公告)号：US5981273A
公开(公告)日：1999-11-09
申请号：US450417
申请日：1995-05-25
申请人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
发明人： David T. Curiel , Max L. Birnstiel , Matthew Cotten , Ernst Wagner , Kurt Zatloukal , Christian Plank , Berndt Oberhauser , Walter G. M. Schmidt
IPC分类号： A61K47/48 , A61K48/00 , C07K14/11 , C07K14/775 , C07K14/79 , C12N15/87 , C07H21/02 , C12N7/00
CPC分类号： A61K47/48776 , A61K48/00 , C07K14/005 , C07K14/775 , C07K14/79 , C12N15/87 , C07K2319/00 , C12N2740/16022
摘要： A composition for the transfection of higher eucaryotic cells, comprising complexes of nucleic acid, a substance having an affinity for nucleic acid and optionally an internalizing factor, contains an endosomolytic agent, e.g. a virus or virus component, which may be conjugated. The endosomolytic agent, which is optionally part of the nucleic acid complex, is internalized into the cells together with the complex and releases the contents of the endosomes into the cytoplasm, thereby increasing the gene transfer capacity. Pharmaceutical preparations, transfection kits and methods for introducing nucleic acid into higher eucaryotic cells by treating the cells with the composition are also disclosed.
摘要翻译：用于转染高等真核细胞的组合物，其包含核酸复合物，对核酸和任选的内在因子具有亲和力的物质，含有内体细胞溶解剂可以结合的病毒或病毒组分。任选地是核酸复合物的一部分的内体细胞溶解剂与复合物一起被内化到细胞中，并将内体的内容物释放到细胞质中，从而增加基因转移能力。还公开了药物制剂，转染试剂盒和通过用组合物处理细胞将核酸引入高级真核细胞的方法。

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