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    • 6. 发明授权
    • Optical substrate for enhanced detectability of fluorescence
    • 用于增强荧光检测性的光学基底
    • US06177990B1
    • 2001-01-23
    • US09468750
    • 1999-12-21
    • Robert C. KainEric G. MarasonRichard F. Johnston
    • Robert C. KainEric G. MarasonRichard F. Johnston
    • G01N100
    • G01N21/6456G01N21/6428G01N21/6452
    • A sample substrate for use in a fluorescence imaging system includes a rigid base with a specularly reflective surface, typically metal, on which is deposited a transparent coating layer. The coating layer has a thickness selected so that a particular fluorescence excitation wavelength, corresponding to a specified fluorescent constituent to be sought in sample material, has an optical path from the top of the coating layer to the reflecting surface in the base of substantially an odd multiple of one-quarter wavelength, so that the standing wave of the fluorescence excitation wavelength of light incident on the substrate has an antinode located at or near where sample material would be disposed on top of the coating layer. This maximizes fluorescence excitation of the sample on the reflective substrate. The transparent coating layer may be a dielectric material (e.g. silica) or may be a multilayer structure with a top layer of biologically active material for binding a specified sample constituent.
    • 用于荧光成像系统的样品衬底包括具有镜面反射表面(通常为金属)的刚性基底,其上沉积有透明涂层。 涂层具有选择的厚度,使得对应于在样品材料中寻找的特定荧光成分的特定荧光激发波长具有从涂层的顶部到基底中的反射表面的基本上奇数的光路 使得入射到基板上的光的荧光激发波长的驻波具有位于或附近的样品材料将设置在涂层顶部上的波腹。 这使得样品在反射基板上的荧光激发最大化。 透明涂层可以是介电材料(例如二氧化硅),也可以是具有用于结合特定样品成分的生物活性材料顶层的多层结构。
    • 9. 发明授权
    • Optical substrate for enhanced detectability of fluorescence
    • 用于增强荧光检测性的光学基底
    • US6008892A
    • 1999-12-28
    • US864363
    • 1997-05-23
    • Robert C. KainEric G. MarasonRichard F. Johnston
    • Robert C. KainEric G. MarasonRichard F. Johnston
    • G01N21/01G01N21/64G01N1/00
    • G01N21/6456G01N21/6452G01N21/6428
    • A sample substrate for use in a fluorescence imaging system includes a rigid base with a specularly reflective surface, typically metal, on which is deposited a transparent coating layer. The coating layer has a thickness selected so that a particular fluorescence excitation wavelength, corresponding to a specified fluorescent constituent to be sought in sample material, has an optical path from the top of the coating layer to the reflecting surface in the base of substantially an odd multiple of one-quarter wavelength, so that the standing wave of the fluorescence excitation wavelength of light incident on the substrate has an antinode located at or near where sample material would be disposed on top of the coating layer. This maximizes fluorescence excitation of the sample on the reflective substrate. The transparent coating layer may be a dielectric material (e.g. silica) or may be a multilayer structure with a top layer of biologically active material for binding a specified sample constituent.
    • 用于荧光成像系统的样品衬底包括具有镜面反射表面(通常为金属)的刚性基底,其上沉积有透明涂层。 涂层具有选择的厚度,使得对应于在样品材料中寻找的特定荧光成分的特定荧光激发波长具有从涂层的顶部到基底中的反射表面的基本上奇数的光路 使得入射到基板上的光的荧光激发波长的驻波具有位于或附近的样品材料将设置在涂层顶部上的波腹。 这使得样品在反射基板上的荧光激发最大化。 透明涂层可以是介电材料(例如二氧化硅),也可以是具有用于结合特定样品成分的生物活性材料顶层的多层结构。