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1. 发明授权

US11845981B2 Delayed pyrophosphorolysis activated polymerization 有权
公开(公告)号：US11845981B2
公开(公告)日：2023-12-19
申请号：US17586596
申请日：2022-01-27
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： C12Q1/6853 , C12Q1/686 , C12Q1/6858
CPC分类号： C12Q1/6853 , C12Q1/686 , C12Q2525/186 , C12Q2565/301
摘要： A minimal-copy-ratio of templates is a problem in detecting early stage cancer where minimal copies of somatic cancer-specific mutations are targeted in the presence of large copies of wildtype genome DNA, commonly a 1/10,000 or even less minimal-copy-ratios between the mutant target and wildtype control templates. To overcome this problem, delayed pyrophosphorolysis activated polymerization (delayed-PAP) was developed which can delay product accumulation of the wildtype control to a much later time or cycle, such as by 15 cycles or by 30,000 folds. In the multiplex format, delayed-PAP is particularly useful to amplify not only the wildtype control but also mutant target templates accurately and consistently in the minimal-copy-ratio situation.

2. 发明申请

US20200150047A1 Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid 审中-公开
公开(公告)号：US20200150047A1
公开(公告)日：2020-05-14
申请号：US16658029
申请日：2019-10-19
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： G01N21/76 , C12Q1/6844
摘要： A new fluorescence detection method called pyrophosphorolysis activated fluorescence was developed to measure PAP amplification of nucleic acid. A fluorophore-quencher dual-attached blocked primer was used for PAP which has a fluorophore attached to a nucleotide in the internal region or at the 5′ end and a quencher attached to a blocked nucleotide at the 3′ end. Multiple fluorophore-quencher dual-labeled blocked primers were also used for multiplex PAP, which are attached with different fluorophores to distinguish multiple templates in a reaction.

3. 发明授权

US10619197B2 Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization 有权
公开(公告)号：US10619197B2
公开(公告)日：2020-04-14
申请号：US15825114
申请日：2017-11-29
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： C12Q1/68 , C12Q1/6848 , C12Q1/6844
CPC分类号： C12Q1/6848 , C12Q1/6844 , C12Q1/6844 , C12Q2527/125
摘要： The invention provides a method for lyophilizing integrated composition of pyrophosphorolysis activated polymerization (PAP) in an aqueous solution. It also provides lyophilized integrated PAP composition. Except for nucleic acid template, the integrated composition contains all components. For manipulation, simply add nucleic acid template in an aqueous solution to start amplification. In addition to the easy manipulation, the lyophilized integrated composition can be stored for prolonged period at ambiguous temperature.

4. 发明申请

US20190271035A1 Multiplex pyrophosphorolysis activated polymerization to amplify multiple almost-sequence-identical templates in a single reaction 审中-公开
公开(公告)号：US20190271035A1
公开(公告)日：2019-09-05
申请号：US16408442
申请日：2019-05-09
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Tony Zhou Lee , Qiang Liu
IPC分类号： C12Q1/6853 , C12Q1/6848 , C12Q1/6858
摘要： Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.

5. 发明申请

US20180073067A1 Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization 审中-公开
公开(公告)号：US20180073067A1
公开(公告)日：2018-03-15
申请号：US15825114
申请日：2017-11-29
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6844 , C12Q2527/125
摘要： The invention provides a method for lyophilizing integrated composition of pyrophosphorolysis activated polymerization (PAP) in an aqueous solution. It also provides lyophilized integrated PAP composition. Except for nucleic acid template, the integrated composition contains all components. For manipulation, simply add nucleic acid template in an aqueous solution to start amplification. In addition to the easy manipulation, the lyophilized integrated composition can be stored for prolonged period at ambiguous temperature.

6. 发明授权

US09470205B2 Wind turbine blades with layered, multi-component spars, and associated systems and methods 有权
标题翻译：具有分层，多组分翼梁的风力涡轮机叶片以及相关的系统和方法
公开(公告)号：US09470205B2
公开(公告)日：2016-10-18
申请号：US13802590
申请日：2013-03-13
申请人： Qiang Liu , Myles L. Baker
发明人： Qiang Liu , Myles L. Baker
IPC分类号： F03D1/06 , F03D1/00 , B29C70/52 , B29L31/08 , B29C65/48 , B29C65/00
CPC分类号： F03D1/0675 , B29C65/48 , B29C66/1248 , B29C66/43 , B29C66/721 , B29C70/52 , B29L2031/085 , F03D13/10 , F05B2240/302 , Y02E10/721 , Y02E10/728 , Y02P70/523 , Y10T29/49337
摘要： Wind turbine blades with layered, multi-component spars, and associated systems and methods are disclosed. A wind turbine blade system in accordance with a particular embodiment includes a first blade segment having a first spar element that includes first planks having a first thickness and a first plank composition, and a second blade segment having a second spar element that includes second planks having a second thickness and a second plank composition different than the first plank composition. The second blade segment is joined to the first blade segment at a joint, and, in particular embodiments, an overall product of thickness and elastic modulus of the first planks is approximately equal to an overall product of thickness and elastic modulus for the second planks.
摘要翻译：公开了具有分层，多组分翼梁以及相关系统和方法的风力涡轮机叶片。根据特定实施例的风力涡轮机叶片系统包括具有第一翼梁元件的第一叶片段，所述第一翼梁元件包括具有第一厚度的第一木板和第一木板组合物，以及具有第二翼梁元件的第二叶片段，所述第二翼梁元件包括第二木板，第二厚度和与第一板组合物不同的第二板组合物。第二刀片段在接头处连接到第一刀片段，并且在具体实施例中，第一板的厚度和弹性模量的总体乘积近似等于第二板的厚度和弹性模量的总积。

7. 发明申请

US20150334588A1 METHODS AND APPARATUSES FOR FAST RECOVERY 有权
标题翻译：快速恢复的方法和设备
公开(公告)号：US20150334588A1
公开(公告)日：2015-11-19
申请号：US14759057
申请日：2014-01-06
申请人： Xinwei CUI , Chenhao GONG , Qiang LIU , Xi LI , MEDIATEK SINGAPORE PTE. LTD.
发明人： Xinwei CUI , Chenhao GONG , Qiang LIU , Xi LI
IPC分类号： H04W24/08 , H04W36/00 , H04W36/30
CPC分类号： H04W24/08 , H04W36/0061 , H04W36/14 , H04W36/26 , H04W36/30
摘要： The invention introduces a method and an apparatus for fast recovery. The fast recovery method comprises: evaluating data download qualities of non-current RATs (Radio Access Technologies) to obtain a candidate RAT list; determining whether a PS (Packet Service) download quality of a current RAT becomes worse; and if the PS download quality of the current RAT becomes worse, selecting one RAT from the candidate RAT list and switching to the selected RAT. The method and the apparatus for fast recovery introduced by the invention realize the seamless RAT switch and maintain the continuous data transmission.
摘要翻译：本发明引入了快速恢复的方法和装置。快速恢复方法包括：评估非当前RAT（无线接入技术）的数据下载质量以获得候选RAT列表; 确定当前RAT的PS（分组服务）下载质量是否变差; 并且如果当前RAT的PS下载质量变差，则从候选RAT列表中选择一个RAT并切换到所选择的RAT。本发明引入的快速恢复方法和装置实现无缝RAT切换，保持连续数据传输。

8. 发明授权

US09178826B2 Method and apparatus for scheduling communication traffic in ATCA-based equipment 有权
标题翻译：用于调度基于ATCA的设备中的通信业务的方法和装置
公开(公告)号：US09178826B2
公开(公告)日：2015-11-03
申请号：US13820798
申请日：2011-08-24
申请人： Caixia Chi , Fang Liu , Qiang Liu , Dong Yan , Bin Wang
发明人： Caixia Chi , Fang Liu , Qiang Liu , Dong Yan , Bin Wang
IPC分类号： H04L12/26 , H04L12/803 , H04L12/851 , H04L12/863 , H04L29/08
CPC分类号： H04L43/0894 , H04L43/062 , H04L43/067 , H04L43/0835 , H04L47/24 , H04L47/2425 , H04L47/54 , H04L67/10
摘要： A method for scheduling communication traffic in an Advanced Telecom Computing Architecture based equipment and a corresponding apparatus are provided. In the method, communication traffic information of traffic processing means in the equipment is collected; based on the collected communication traffic information, a traffic distribution rule for the traffic processing means in next time period is generated; and based on the generated traffic distribution rule, incoming communication traffic of the equipment is scheduled. The method of the invention considers the quality of service and contracted capacity of the whole equipment and the dynamic processing capability and running environment of the traffic processing means, and can balance the loads of the respective traffic processing means in the equipment with asymmetric multi-application configuration well by adaptively adjusting the distribution of the communication traffic, to guarantee the quality of service of the whole equipment.
摘要翻译：提供了一种用于在基于高级电信计算架构的设备和相应设备中调度通信业务的方法。在该方法中，收集设备中的业务处理装置的通信业务信息; 基于所收集的通信交通信息，生成下一时间段中的交通处理装置的交通分配规则; 并基于生成的流量分配规则，调度设备的进入通信流量。本发明的方法考虑了整个设备的服务质量和承包能力以及交通处理手段的动态处理能力和运行环境，并且可以平衡不对称多应用的设备中各个业务处理手段的负载配置好，通过自适应调整通信流量的分布，保证整个设备的服务质量。

9. 发明授权

US09163230B2 Chromatography column and method for isolating nucleic acid 有权
标题翻译：色谱柱和分离核酸的方法
公开(公告)号：US09163230B2
公开(公告)日：2015-10-20
申请号：US13918979
申请日：2013-06-16
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12N15/10
CPC分类号： C12N15/101 , C07H21/00 , C07H21/02 , C07H21/04
摘要： The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane. Upon flowing a nucleic acid-containing solution through the first anion exchange membrane, the nucleic acid binds to and then elutes from the first membrane. The eluted solution then flows serially through the second silica membrane, which the nucleic acid binds to and then elutes from. Due to this novel serial coupled double-layer principle, the present invention is particularly suitable for co-isolating RNA and DNA, for isolating nucleic acid embraced by proteins, e.g., viruses, and for isolating diluted nucleic acid in a large volume, e.g., plasma. In addition, the eluted nucleic acid is ready for downstream applications.
摘要翻译：本发明提供色谱柱和分离核酸分子的方法。在一个实施方案中，本发明提供了第一阴离子交换膜和第二串联偶联二氧化硅膜的双层柱。当使含核酸的溶液流过第一阴离子交换膜时，核酸与第一膜结合并然后从第一膜洗脱。然后洗脱的溶液顺序流过第二个二氧化硅膜，核酸结合然后从其中洗脱出来。由于这种新颖的串联耦合双层原理，本发明特别适用于共分离RNA和DNA，用于分离蛋白质（例如病毒）所包围的核酸，并用于大体积分离稀释的核酸，例如，等离子体。此外，洗脱的核酸准备用于下游应用。

10. 发明授权

US09133491B2 Pyrophosphorolysis-activated polymerization (PAP) using ribonucleic acid (RNA) template 有权
公开(公告)号：US09133491B2
公开(公告)日：2015-09-15
申请号：US13918980
申请日：2013-06-16
申请人： Shaofeng Ding , Qiang Liu
发明人： Shaofeng Ding , Qiang Liu
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/68 , C12Q1/6844 , C12Q2521/107 , C12Q2525/121 , C12Q2525/186 , C12Q2565/301
摘要： A new method of RNA-PAP was developed that can directly amplify RNA template without additional treatment. RNA-PAP brings in a new mechanism for amplification of RNA template in which RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization are serially coupled using 3′ blocked primers. Due to this serial coupling, RNA-PAP has high selectivity against mismatches on the RNA template, providing highly specific amplification of RNA template. In addition, mutant polymerases were genetically engineered for higher efficiency of RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization.

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