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1. 发明申请

US20050214764A1 Method for identifying, quantifying and/or characterizing an analyte 审中-公开
标题翻译：识别，定量和/或表征分析物的方法
公开(公告)号：US20050214764A1
公开(公告)日：2005-09-29
申请号：US10506759
申请日：2003-03-04
申请人： Emil Palecek , Hans Kosak
发明人： Emil Palecek , Hans Kosak
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6825 , C12Q1/6823 , C12Q1/6827 , C12Q2563/113 , C12Q2537/143 , C12Q2545/114
摘要： The invention relates to a method for identifying, quantifying and/or characterizing an analyte (10) contained in a first liquid. Said method is characterized by the following steps: a) bringing the analyte (10) into contact with a first probe (20) and with a second probe (22), each probe having an affinity to the analyte (20), and incubating the analyte. The affinity of the first probe (20) is effected by a specific affinity to at least one first binding site (12) of the analyte (20), and the incubating ensues under conditions under which the first probe (20), and the second probe (22) bind to the analyte (10). Other steps include: b) marking the first probe (20) with at least one first marker (24) that can be identified in an electrochemically specific manner at least when the probe (22) cannot already be identified in an electrochemically specific manner; c) marking the second probe (22) with at least one second marker (26) that can be identified in an electrochemically specific manner at least when this probe cannot already be identified in an electrochemically specific manner; d) separating the first probe (20) and second probe (22) that are bound to the analyte (10); e) detecting a first electrochemical signal Si1, which is caused by the separated first probe (20) or by the first marker (24) and detecting a second electrochemical signal Si2 caused by the separated second probe (22) or by the second marker (26), and; f) identifying, quantifying and/or characterizing the analyte (10) by using a ratio between the first Si1 and the second signal Si2.
摘要翻译：本发明涉及用于识别，定量和/或表征包含在第一液体中的分析物（10）的方法。所述方法的特征在于以下步骤：a）使分析物（10）与第一探针（20）和第二探针（22）接触，每个探针对分析物（20）具有亲和力，并且将分析物。第一探针（20）的亲和力通过对分析物（20）的至少一个第一结合位点（12）的特异性亲和力进行，并且在第一探针（20）和第二探针探针（22）与分析物（10）结合。其他步骤包括：b）用至少一个第一标记（24）标记第一探针（20），至少一个第一标记（24）可以至少当探针（22）不能以电化学特异性方式识别时以电化学特异性方式被识别; c）用能够以电化学特异性方式识别的至少一个第二标记（26）来标记第二探针（22），至少当该探针不能以电化学特异性方式被识别时; d）分离与分析物（10）结合的第一探针（20）和第二探针（22）; e）检测由分离的第一探针（20）或第一标记（24）引起的第一电化学信号Si 1，并检测由分离的第二探针（22）引起的第二电化学信号Si 2或第二电化学信号Si 标记（26）和 f）通过使用第一Si 1和第二信号Si 2之间的比率来识别，定量和/或表征分析物（10）。

2. 发明申请

US20070048194A1 Use of a disposable container, microfluidic device and method for processing molecules 审中-公开
标题翻译：使用一次性容器，微流体装置和处理分子的方法
公开(公告)号：US20070048194A1
公开(公告)日：2007-03-01
申请号：US10563279
申请日：2003-07-15
申请人： Jurgen Schulein , Bjoern Grassel , Juergen Krause , Roland Barten , Dirk Kuhlmeier , Joerg Hassmann , Hans Kosak
发明人： Jurgen Schulein , Bjoern Grassel , Juergen Krause , Roland Barten , Dirk Kuhlmeier , Joerg Hassmann , Hans Kosak
IPC分类号： B01L3/00
CPC分类号： B01L3/502715 , B01L3/565 , B01L2200/027 , B01L2200/04 , B01L2200/141 , B01L2200/16 , B01L2300/021 , B01L2300/022 , B01L2400/0478
摘要： The invention relates to the use of a disposable container comprising a cylindcr (1) containing a plunger (2, 24) that is displaceably guided in said cylinder and a connection (3) that is provided on a first end (E1) of the cylinder (1) lying opposite the plunger (2, 24), said container being designed to create a difference in pressure in a microfluidic device (13) to hold (6) an agent (F, P) for processing molecules and/or to act as a reaction vessel.
摘要翻译：本发明涉及一次性容器的使用，该容器包括一个包含柱塞（2,24）的圆柱形件（1），该柱塞可移动地被引导到所述缸体中;以及连接件（3），其设在所述缸体的第一端（E 1）上所述容器设计成在微流体装置（13）中产生压力差，以保持（6）用于处理分子的试剂（F，P）和/或作为反应容器。

3. 发明申请

US20050106684A1 Method and device for carrying out a reaction 审中-公开
标题翻译：用于进行反应的方法和装置
公开(公告)号：US20050106684A1
公开(公告)日：2005-05-19
申请号：US10970931
申请日：2004-10-22
申请人： Jorg Hassmann , Hans Kosak , Dirk Kuhlmeier , Jurgen Krause , Markus Neugebauer , Ugur Ulker
发明人： Jorg Hassmann , Hans Kosak , Dirk Kuhlmeier , Jurgen Krause , Markus Neugebauer , Ugur Ulker
IPC分类号： B01L3/00 , B01L7/00 , C12Q1/68 , C12P19/34
CPC分类号： B01L3/50851 , B01L3/5027 , B01L7/52 , B01L2200/0684 , B01L2300/0809 , B01L2300/0838 , B01L2300/0887 , B01L2300/1822 , B01L2400/0487
摘要： The invention relates to a method for carrying out a reaction in an unmoved liquid in a capillary which is open at at least one end, with gas bubbles forming in the liquid, with a detergent being added to the liquid for the purpose of changing at least one property of the gas bubbles which are being formed and with the capillary being arranged, or having been arranged, at at least an angle to the horizontal which is such that the gas bubbles in the capillary ascend to a surface of the liquid.
摘要翻译：本发明涉及一种用于在毛细管中进行反应的方法，该毛细管在至少一端是开放的，在液体中形成气泡，洗涤剂被添加到液体中以至少改变正在形成的气泡的一个性质和毛细管被布置，或者被布置成与水平面至少一个角度，使得毛细管中的气泡上升到液体的表面。

4. 发明授权

US06261809B1 Method for marking solid, liquid or gaseous substances 有权
标题翻译：固体，液体或气体物质的标记方法
公开(公告)号：US06261809B1
公开(公告)日：2001-07-17
申请号：US09486884
申请日：2000-03-03
申请人： Wolf Bertling , Hans Kosak
发明人： Wolf Bertling , Hans Kosak
IPC分类号： C12P1934
CPC分类号： G09F3/00 , C12Q1/68 , C12Q2563/185
摘要： The invention relates to a method for marking solid, liquid or gaseous substances, whereby the substance to be marked is provided with at least one synthetically produced nucleic acid sequence. Said nucleic acid sequence contains a first sequence section constructed with the 5′ terminal end, a second sequence comprised of at least two bases and connected to said nucleic acid sequence, and a third sequence section constructed with the 3′ terminal end and connected to the said nucleic acid sequence. In order to simplify the identification of the marking, the invention provides that a first primer group is used with a first primer section corresponding to the first sequence section and a second primer group is used with a third primer section corresponding to the third sequence section. Every primer group four comprises differing primer variants in at least one additional respective base which is provided on the end so that exactly one primer variant of the first primer group together with exactly one primer variant of the second primer group is complimentary to the nucleic acid sequence.
摘要翻译：本发明涉及一种用于标记固体，液体或气体物质的方法，由此待标记的物质具有至少一个合成产生的核酸序列。所述核酸序列包含由5'末端构建的第一序列部分，由至少两个碱基组成并连接到所述核酸序列的第二序列，以及由3'末端构建并与所述核酸序列。为了简化标记的识别，本发明提供了使用与第一序列部分相对应的第一引物部分的第一引物组和与第三序列部分相对应的第三引物部分使用第二引物组。每个引物组4在至少一个额外的各自的碱基中包含不同的引物变体，其在末端提供，使得第一引物组的正好一个引物变体与第二引物组的正义一个引物变体与核酸序列互补。

5. 发明申请

US20060024674A1 Method for conducting the parallel identification of different nucleic acids 审中-公开
标题翻译：进行不同核酸平行鉴定的方法
公开(公告)号：US20060024674A1
公开(公告)日：2006-02-02
申请号：US10534711
申请日：2003-11-13
申请人： Ronald Barten , Dirk Kuhlmeier , Anja Weiland , Hans Kosak , Jorg Hassmann
发明人： Ronald Barten , Dirk Kuhlmeier , Anja Weiland , Hans Kosak , Jorg Hassmann
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6853 , C12Q2525/155 , C12Q2531/107
摘要： The invention relates to a method for conducting the parallel identification of different nucleic acids S. When nucleic acids S are present, primers are lengthened each of which having an intermediate segment z. The intermediate segment z is amplified by using a second primer pair and is specifically identified by hybridizing with a probe So.
摘要翻译：本发明涉及用于进行不同核酸S的平行鉴定的方法。当存在核酸S时，引物延长其中每一个具有中间片段z。通过使用第二引物对来扩增中间片段z，并通过与探针So杂交来特异性鉴定。

6. 发明申请

US20050214532A1 Secueity thread for the forgery-proof making of objects 审中-公开
标题翻译：防伪物品的保护线
公开(公告)号：US20050214532A1
公开(公告)日：2005-09-29
申请号：US10477158
申请日：2002-05-08
申请人： Hans Kosak , Andre Josten
发明人： Hans Kosak , Andre Josten
IPC分类号： G07D7/14 , D02G3/00
CPC分类号： D06M15/01 , C12Q1/6813 , G07D7/14 , Y10T428/2913 , C12Q2563/185 , C12Q2563/155 , C12Q2527/125
摘要： The invention relates to a security thread for the forgery-proof marking of objects, comprising at least one fibre (F) with nucleic acid molecules (N) bonded with the one end thereof to a fibre suface and the fibre (F). The other end of the nucleic acid molecule (N) is free such that complementary nucleic acid molecules (N′) may bind to the nucleic acid molecule (N).
摘要翻译：本发明涉及一种用于物体的防伪标记的安全线，包括至少一根纤维（F），其一端与纤维表面和纤维（F）结合的核酸分子（N）。核酸分子（N）的另一端是游离的，使得互补核酸分子（N'）可以结合核酸分子（N）。

7. 发明授权

US07276369B2 Method for detecting a substance and microtiter plate 失效
标题翻译：检测物质和微量滴定板的方法
公开(公告)号：US07276369B2
公开(公告)日：2007-10-02
申请号：US10479368
申请日：2002-05-29
申请人： Hans Kosak
发明人： Hans Kosak
IPC分类号： C12M1/34
CPC分类号： B01L3/5085 , G01N27/44726 , G01N33/54373
摘要： The invention relates to a method for detecting an analyte A in a liquid, comprising the following steps: (a) a solution containing an analytical reagent N is provided in a container B; (b) the analyte A is added to the solution, (c) an electrical field eF which acts upon the solution is applied by means of electrodes disposed outside the container B, whereby a modification occurs in the concentration of a substance which is specific with respect to the presence of the analyte A in a region M of the container B and (d) the modification of said concentration is optically detected.
摘要翻译：本发明涉及一种用于检测液体中分析物A的方法，包括以下步骤：（a）在容器B中提供含有分析试剂N的溶液; （b）将分析物A添加到溶液中，（c）通过设置在容器B外部的电极施加作用于溶液的电场eF，由此在特定于考虑到容器B的区域M中分析物A的存在，以及（d）对所述浓度的改变进行光学检测。

8. 发明申请

US20060222569A1 Device and method for the preparation of analyte comprising liquids 审中-公开
标题翻译：用于制备包含液体的分析物的装置和方法
公开(公告)号：US20060222569A1
公开(公告)日：2006-10-05
申请号：US10553999
申请日：2004-04-19
申请人： Roland Barten , Jurgen Krause , Hans Kosak
发明人： Roland Barten , Jurgen Krause , Hans Kosak
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01L2200/026 , B01L2200/0668 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0816 , B01L2300/0867 , B01L2400/0478 , B01L2400/0655 , G01N1/28
摘要： The present invention relates to a device, a kit and a method for the contamination-free preparation of analyte, in particular biopolymer comprising liquids. The device comprises wo chambers (2, 3) having a reversibly changeable volume.
摘要翻译：本发明涉及用于无污染制备分析物的装置，试剂盒和方法，特别是包含液体的生物聚合物。该装置包括具有可逆变化体积的窝室（2,3）。

9. 发明申请

US20110046205A1 METHOD FOR DISSOLVING CHARGED NUCLEIC ACID IN AN ORGANIC LIQUID 审中-公开
标题翻译：用于溶解有机液体中充电核酸的方法
公开(公告)号：US20110046205A1
公开(公告)日：2011-02-24
申请号：US12377769
申请日：2007-08-16
申请人： Hans Kosak , Andre Josten
发明人： Hans Kosak , Andre Josten
IPC分类号： A61K31/7084 , C07H21/00 , A61P43/00 , C10L1/238 , C10L5/00 , G01N33/00 , C12Q1/68 , G01N33/02
CPC分类号： C12N15/1003 , Y10T436/143333
摘要： The invention relates to a method for dissolving charged nucleic acids in an organic first liquid which is immiscible with water. The method comprises the following steps: a) providing a solution of the nucleic acids in an aqueous second liquid, b) precipitating the nucleic acids by adding a complexing agent to the second liquid, the complexing agent forming complexes with the nucleic acids that are insoluble in the second liquid, c) removing the complexes from the second liquid, d) dissolving the complexes in a third liquid which consists of an amphiphilic compound or which contains an amphiphilic compound, and e) mixing the third liquid with the first liquid.
摘要翻译：本发明涉及将带电核酸溶解在与水不混溶的有机第一液体中的方法。该方法包括以下步骤：a）提供核酸在水性第二液体中的溶液，b）通过向第二液体中加入络合剂来沉淀核酸，所述络合剂与不溶性核酸形成复合物在所述第二液体中，c）从所述第二液体中除去所述络合物，d）将所述络合物溶解在由两亲性化合物组成或含有两亲性化合物的第三液体中，和e）将所述第三液体与所述第一液体混合。

10. 发明申请

US20060011474A1 Device for detecting an analyte 审中-公开
标题翻译：用于检测分析物的装置
公开(公告)号：US20060011474A1
公开(公告)日：2006-01-19
申请号：US10518498
申请日：2003-06-27
申请人： Jurgen Schulein , Christine Kugler , Burcu Meric , Hans Kosak , Jorg Hassmann , Bjorn Grassl , Dirk Kuhlmeier
发明人： Jurgen Schulein , Christine Kugler , Burcu Meric , Hans Kosak , Jorg Hassmann , Bjorn Grassl , Dirk Kuhlmeier
IPC分类号： G01N27/26 , G01N33/487
CPC分类号： G01N27/3276 , G01N27/308 , Y10T29/49002
摘要： The invention relates to a device (17) for detecting an analyte in a liquid having a multitude of electrodes (15), which are insulated from one another while being arranged on a first side (12) of an electrically non-conductive plate (10) that is impermeable to the liquid. Said electrodes (15) have, at least in part, an analyte-specific coating or analyte-specific molecules. Electrical conductors that pass through the plate (10) can, from a second side (14) of the plate, be electrically contacted and individually discharged. The coating or the molecules has/have a specific affinity to the analytes or to a substance formed due to the presence of the analyte. In addition, the device has no leakages.
摘要翻译：本发明涉及一种用于检测具有多个电极（15）的液体中的分析物的装置（17），它们彼此绝缘，同时被布置在非导电板（10）的第一侧（12）上）对液体是不透水的。所述电极（15）至少部分具有分析物特异性涂层或分析物特异性分子。穿过板（10）的电导体可以从板的第二侧（14）电接触并单独排出。涂层或分子对分析物或由于分析物的存在形成的物质具有特异性亲和力。此外，该设备没有泄漏。

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