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1. 发明授权

US4278763A Diagnostic agents for the detection of proteolytic enzymes 失效
标题翻译：用于检测蛋白水解酶的诊断剂
公开(公告)号：US4278763A
公开(公告)日：1981-07-14
申请号：US99180
申请日：1979-11-30
申请人： Dieter Berger , Franz Braun , Werner Guthlein , Manfred Kuhr , Wolfgang Werner
发明人： Dieter Berger , Franz Braun , Werner Guthlein , Manfred Kuhr , Wolfgang Werner
IPC分类号： C07D209/36 , C07D209/12 , C07D333/64 , C07K1/113 , C07K5/04 , C07K7/06 , C12Q1/37 , G01N33/48 , G01N33/50 , C12Q1/38
CPC分类号： C12Q1/37 , C12Q2334/50
摘要： The present invention provides a diagnostic agent for the detection of proteolytic enzymes, comprising an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet, containing at least one chromogen and an appropriate buffer substance, wherein the chromogen used is an indoxyl- and/or thioindoxyl-amino acid ester and/or peptide ester of the general formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, lower alkoxy, aryl, aralkyl, aralkoxy, hydroxyl, carboxy, carboxy lower alkoxy, aralkoxycarbonyl, aralkyloxycarbonyl lower alkoxy, nitro or lower acylamino radicals, whereby two adjacent substituents can also represent an optionally halogenated fused benzene ring, X is a sulphur atom or an imino group optionally substituted by a lower alkyl, aryl, aralkyl or acyl radical, A is an amino acid or peptide residue and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom.The present invention also provides new compounds of the above-given general formula and processes for the preparation thereof.Furthermore, the present invention is concerned with the use of the compounds of the above-given general formula for the production of diagnostic agents for the detection of proteolytic enzymes.
摘要翻译：本发明提供了用于检测蛋白水解酶的诊断剂，其包含含有至少一种色原体和适当的缓冲物质的吸收剂载体，膜层，粉末混合物，冻干物，溶液或试剂片剂，其中使用的色原是通式为：其中R 1，R 2，R 3和R 4可以相同或不同，为氢或卤素原子的通式如下的吲哚氧基和/或硫代吲哚氧基 - 氨基酸酯和/或肽酯：低级烷基，低级烷氧基，芳基，芳烷基，芳烷氧基，羟基，羧基，羧基低级烷氧基，芳烷氧基羰基，芳烷氧基羰基低级烷氧基，硝基或低级酰基氨基，其中两个相邻的取代基也可以表示任选卤代的稠合苯环，X是硫原子或任选被低级烷基，芳基，芳烷基或酰基取代的亚氨基，A是氨基酸或肽残基，B是肽化学中常规的氮保护基或从其衍生。本发明还提供了上述给出的通式的新化合物及其制备方法。此外，本发明涉及上述给出的通式的化合物用于产生用于检测蛋白水解酶的诊断剂的用途。

2. 发明授权

US4442033A Azo dyestuff for use as a chromogen in detecting leukocytes 失效
标题翻译：偶氮染料用作检测白细胞的色原体
公开(公告)号：US4442033A
公开(公告)日：1984-04-10
申请号：US271342
申请日：1981-06-08
申请人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Manfred Kuhr
发明人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Manfred Kuhr
IPC分类号： C09B43/20 , C09B43/18 , C09B43/28 , G01N33/50 , C09B29/036 , C09B29/042 , C09B29/15
CPC分类号： C09B43/18 , G01N33/5094
摘要： Azo dyestuff ester of the formulaA--N.dbd.N--B(OR).sub.nwhereinA is a five- or six-membered heterocyclic radical of up to 2 hetero ring atoms selected from nitrogen, sulfur and oxygen, and wherein the heterocyclic ring may be substituted with at least one member of the group selected from halogen, lower alkyl and lower alkoxy; orA is phenyl substituted by at least one member of the group selected from lower alkyl, lower alkoxy, nitro, sulphanato and acylamino;B is benzene, naphthalene or quinoline, optionally substituted with one or two radicals selected from sulphanato, lower alkoxy and lower alkoxypoly-alkyleneoxy;R is a carboxylic acid residue or an amino acid or peptide residue having a nitrogen protective group; andn is 1 or 2.This dyestuff is particularly useful as a chromogen in a diagnostic composition for detecting leukocytes in body fluids.
摘要翻译：式AN = NB（OR）n的偶氮染料酯，其中A是选自氮，硫和氧的至多2个杂环原子的5或6元杂环基，并且其中杂环可以被选自卤素，低级烷基和低级烷氧基的基团中的至少一个成员; 或A是被选自低级烷基，低级烷氧基，硝基，磺酰基和酰氨基中的至少一个基团取代的苯基; B是苯，萘或喹啉，任选被一个或两个选自磺酰基，低级烷氧基和低级烷氧基聚 - 亚烷氧基的基团取代; R是具有氮保护基团的羧酸残基或氨基酸或肽残基; 并且n为1或2.该染料特别可用作用于检测体液中白细胞的诊断组合物中的色原体。

3. 发明授权

US4296202A Diagnostic composition for the detection of leukocytes and chromogens useful therein 失效
标题翻译：用于检测其中有用的白细胞和色原体的诊断组合物
公开(公告)号：US4296202A
公开(公告)日：1981-10-20
申请号：US63211
申请日：1979-08-02
申请人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Manfred Kuhr
发明人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Manfred Kuhr
IPC分类号： C09B43/20 , C09B43/18 , C09B43/28 , G01N33/50
CPC分类号： C09B43/18 , G01N33/5094
摘要： Novel diagnostic composition for the detection of leukocytes in body fluids, comprising an absorbent carrier impregnated with a buffer substance and a chromgen, wherein the chromogen is an azo dyestuff ester of the formulaA--N.dbd.N--B(OR).sub.nwhereinA is a five- or six-membered heterocyclic radical of up to 2 hetero ring atoms selected from nitrogen, sulfur and oxygen, and wherein the heterocyclic ring may be substituted with at least one member of the group selected from halogen, lower alkyl and lower alkoxy; orA is phenyl substituted by at least one member of the group selected from lower alkyl, lower alkoxy, nitro, sulphanato and acylamino;B is benzene, naphthalene or quinolone, optionally substituted with one or two radicals selected from sulphanato, lower alkoxy and lower alkoxypoly-alkyleneoxy;R is a carboxylic acid residue or an amino acid or peptide residue having a nitrogen protective group; andn is 1 or 2.
摘要翻译：用于检测体液中白细胞的新型诊断组合物，其包含浸渍有缓冲物质和染色剂的吸收剂载体，其中所述色原是式AN = NB（OR）n的偶氮染料酯，其中A是五或多至2个选自氮，硫和氧的杂环原子的六元杂环基，其中杂环可以被选自卤素，低级烷基和低级烷氧基中的至少一个基团取代; 或A是被选自低级烷基，低级烷氧基，硝基，磺酰基和酰氨基中的至少一个基团取代的苯基; B是苯，萘或喹诺酮，任选地被一个或两个选自磺酰基，低级烷氧基和低级烷氧基聚 - 亚烷氧基的基团取代; R是具有氮保护基团的羧酸残基或氨基酸或肽残基; n为1或2。

4. 发明授权

US4331760A Diagnostic agent for the detection of leukocytes and chromogens useful therein 失效
标题翻译：用于检测其中有用的白细胞和色素的诊断剂
公开(公告)号：US4331760A
公开(公告)日：1982-05-25
申请号：US42393
申请日：1979-05-25
申请人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Wolfgang Werner
发明人： Dieter Berger , Franz Braun , Gunter Frey , Werner Guthlein , Wolfgang Werner
IPC分类号： G01N33/49 , C07D327/04 , C09B11/00 , C09B11/06 , G01N33/50 , C12Q1/44 , G01N33/48
CPC分类号： G01N33/5094 , C07D327/04 , Y02P20/55
摘要： Diagnostic agent for the detection of leukocytes in body fluids, comprising an absorbent carrier which is impregnated with a chromogen and a buffer substance, wherein the chromogen is a sulfonphthalein ester of the formula ##STR1## wherein R.sub.1 is a carboxylic acid residue optionally substituted by halogen or lower alkoxy; or is an amino acid or peptide residue having a nitrogen protective group;R.sub.2 is a halogen atom or a lower alkyl radical; andR.sub.3 and R.sub.4 are individually selected from hydrogen and halogen.Certain of the chromogen are provided as novel compounds.
摘要翻译：用于检测体液中白细胞的诊断剂，其包含用色素沉积物和缓冲物质浸渍的吸收剂载体，其中所述色原是式IMAMA的磺酞类，其中R1是任选被卤素取代的羧酸残基或低级烷氧基; 或具有氮保护基的氨基酸或肽残基; R2是卤素原子或低级烷基; 并且R 3和R 4分别选自氢和卤素。某些色原提供为新化合物。

5. 发明授权

US4070495A Microscope slide 失效
标题翻译：显微镜幻灯片
公开(公告)号：US4070495A
公开(公告)日：1978-01-24
申请号：US672572
申请日：1976-03-31
申请人： Dieter Berger , Werner Guthlein , Wolfgang Werner , Peter Rieckmann
发明人： Dieter Berger , Werner Guthlein , Wolfgang Werner , Peter Rieckmann
IPC分类号： G01N33/48 , G01N1/30 , G01N33/16 , G01N21/60 , G01N31/22
CPC分类号： G01N1/30
摘要： Microscope slide for blood investigation having a coating of highly purified methylene blue N and highly purified cresyl violet acetate in a weight ratio of from 1:1.5 to 1:5.
摘要翻译：用于血液研究的显微镜载玻片，其重量比为1:1.5至1:5，具有高度纯化的亚甲基蓝N和高度纯化的甲酚紫乙酸酯的涂层。

6. 发明授权

US5260428A Agent for the detection of substances with hydrolase activity 失效
标题翻译：用于检测具有水解酶活性的物质的试剂
公开(公告)号：US5260428A
公开(公告)日：1993-11-09
申请号：US988975
申请日：1993-01-29
申请人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
发明人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
IPC分类号： C12Q1/34 , G01N33/58 , C07H15/24 , C07C43/00 , C07C63/36 , C07C303/00 , C07H15/00
CPC分类号： C12Q1/34 , G01N33/581 , C12Q2334/30 , G01N2333/924
摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidising agent, and the evaluating the resultant colour intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5, R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue. Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.
摘要翻译：一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5，R 6是氢，卤素，羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

7. 发明授权

US4665023A Imidazole derivatives as redox indicators 失效
标题翻译：咪唑衍生物作为氧化还原指标
公开(公告)号：US4665023A
公开(公告)日：1987-05-12
申请号：US717511
申请日：1985-03-29
申请人： Ulfert Deneke , Werner Guthlein , Manfred Kuhr , Hartmut Merdes , Hans-Rudiger Murawski , Hans Wielinger
发明人： Ulfert Deneke , Werner Guthlein , Manfred Kuhr , Hartmut Merdes , Hans-Rudiger Murawski , Hans Wielinger
IPC分类号： C07D233/60 , A61K49/00 , C07D233/54 , C07D233/61 , C07D233/64 , C07D401/04 , C07D403/04 , C07D405/04 , C07D405/14 , C07D455/04 , C07F9/6506 , C12Q1/28 , G01N33/50 , G01N33/68
CPC分类号： C07D233/64 , C07D403/04 , C07D405/04 , C07D455/04 , C12Q1/28 , C12Q2326/00 , Y10T436/206664
摘要： The present invention provides imidazole derivatives of the general formula: ##STR1## wherein R, R.sub.1 and R.sub.2 are defined hereinbelow. The present invention is also concerned with processes for the preparation of these imidazole derivatives and with the use thereof as redox indicators.
摘要翻译：本发明提供以下通式的咪唑衍生物：其中R，R 1和R 2定义如下。本发明还涉及制备这些咪唑衍生物的方法，并将其用作氧化还原指示剂。

8. 发明授权

US5202233A Process for the detection of substances with hydrolase activity 失效
标题翻译：检测具有水解酶活性的物质的方法
公开(公告)号：US5202233A
公开(公告)日：1993-04-13
申请号：US816795
申请日：1992-01-03
申请人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
发明人： Rupert Herrmann , Hans-Joachim Guder , Werner Guthlein , Manfred Kuhr , Johann Berger , Harvey Buck
IPC分类号： C12Q1/34 , G01N33/58
CPC分类号： C12Q1/34 , G01N33/581 , C12Q2334/30 , G01N2333/924 , Y10S435/805
摘要： A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical,R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, andX is a glycosyl, phosphate or acyl residue.Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.
摘要翻译：一种用于通过将样品与水解酶底物和氧化剂混合来检测样品中水解酶活性的物质并评估所得颜色强度的方法，其中作为水解酶底物，使用至少一种式其中R 1是氢或烷氧基，R 2是氢或卤素，氨基或烷氧基或芳烷氧基，可以相同或不同的R 3，R 4，R 5和R 6是氢，卤素，羧基，氨基甲酰基，磺基，氨基烷基，烷氧基，芳烷氧基，烷基羰基或烷氧基羰基，X是糖基，磷酸酯或酰基残基。任选地，R 2和R 3，R 3和R 4，R 4和R 5以及R 5和R 6可以连接形成环系。 R 1和R 2中的至少一个必须是氢，R 1，R 2和R 3中的至少一个不能是氢。 X是β-半乳糖基的化合物是新的。

9. 发明授权

US3988208A 9-Substituted 3-aminocarbazole compounds and test strip indicator compositions 失效
标题翻译： 9-取代的3-氨基咔唑化合物和测试条指示剂组合物
公开(公告)号：US3988208A
公开(公告)日：1976-10-26
申请号：US559273
申请日：1975-03-17
申请人： Wolfgang Werner , Peter Vogel , Hugo Tiedemann , Werner Guthlein
发明人： Wolfgang Werner , Peter Vogel , Hugo Tiedemann , Werner Guthlein
IPC分类号： C07D209/88 , C12Q1/28 , C12Q1/54 , C12K1/04
CPC分类号： C12Q1/54 , C07D209/88 , C12Q1/28 , C12Q2326/00 , C12Q2326/14
摘要： Novel 3-aminocarbazole compounds of the formula: ##SPC1##WhereinX is hydrogen or halogen,Y is oxygen or represents two hydrogen atoms; andR.sub.1 and R.sub.2, which can be the same or different, are hydrogen, lower alkyl or hydroxyalkyl or amino; andN is 1 or 2, with the proviso that when n is2, X, Y, R.sub.1 and R.sub.2 cannot all be hydrogen atoms, as well as the salts thereof with inorganic and organic acids; are outstandingly effective indicators for the determination of glucose and provide stable compositions for such glucose determination.
摘要翻译：新型3-氨基咔唑化合物，具有下式：

10. 发明授权

US4219336A Diagnostic agent and method for the detection of occult blood in feces 失效
标题翻译：用于检测粪便中隐匿性血液的诊断剂和方法
公开(公告)号：US4219336A
公开(公告)日：1980-08-26
申请号：US892359
申请日：1978-03-31
申请人： Werner Guthlein , Hans Wielinger , Walter Rittersdorf , Wolfgang Werner
发明人： Werner Guthlein , Hans Wielinger , Walter Rittersdorf , Wolfgang Werner
IPC分类号： G01N33/50 , A61K36/18 , C07G99/00 , C12Q1/28 , G01N33/72 , G01N33/16
CPC分类号： C12Q1/28 , G01N33/725 , C12Q2326/00 , Y10S436/904
摘要： A diagnostic agent for the detection of occult blood in feces comprising hydrogen peroxide and, as a chromogen, guaiaconic acid A, optionally with a stabilizer. The invention provides, as a novel substance, guaiaconic acid A having a specific extinction E.sub.1 cm.sup.1% at 600 nm of at least 200, determined by the reaction with peroxidase and hydrogen peroxide, having infra-red bands at1600 cm.sup.-1 (m);1505 cm.sup.-1 (v.s.);1260 cm.sup.-1 (s;b);1115 cm.sup.-1 (m);1025 cm.sup.-1 (m); and1200 cm.sup.-1 (s;b)and having an R.sub.F value of 0.45 (toluene/dioxan/glacial acetic acid; 90:25:10 v/v/v).
摘要翻译：用于检测粪便中的潜血的诊断剂，其包含过氧化氢和任选地与稳定剂作为色原，愈创木酸A。本发明提供作为新物质，通过与过氧化物酶和过氧化氢的反应确定在600nm处具有600nm以上的具有特定消光度E1cm1％的愈创木酸A至少为200，在1600cm -1处具有红外线

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