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1. 发明授权

US08039814B2 Luminescence sensor operating in reflection mode 有权
标题翻译：发光传感器以反射模式工作
公开(公告)号：US08039814B2
公开(公告)日：2011-10-18
申请号：US12158451
申请日：2006-12-19
申请人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6428
摘要： A luminescence sensor, such as a luminescence biosensor or a luminescence chemical sensor, includes a substrate having at least one aperture filled with a medium. The aperture has a first lateral dimension larger than the diffraction limit of excitation radiation in the medium, and a second lateral dimension smaller than the diffraction limit of the excitation radiation in the medium. A method is also provided for the detection of luminescence radiation, e.g. fluorescence radiation, generated by at least one optically variable molecule, e.g. fluorophore, in the at least one aperture. Beneficially, the excitation radiation is polarized to suppress the excitation radiation in the apertures. The luminescence sensor and the method are able to detect relatively low concentrations of optically variable molecules, e.g. fluorophores.
摘要翻译：诸如发光生物传感器或发光化学传感器的发光传感器包括具有填充有介质的至少一个孔的基板。该孔具有比介质中的激发辐射的衍射极限大的第一横向尺寸，以及小于介质中的激发辐射的衍射极限的第二横向尺寸。还提供了一种用于检测发光辐射的方法，例如，由至少一个光学可变分子产生的荧光辐射，例如。荧光团，在至少一个孔中。有利地，激发辐射被极化以抑制孔中的激发辐射。发光传感器和方法能够检测相对低浓度的光学可变分子，例如，荧光团

2. 发明申请

US20100019155A1 LUMINESCENCE SENSORS USING SUB-WAVELENGTH APERTURES OR SLITS 审中-公开
标题翻译：使用子波长波形或者倾斜的发光体传感器
公开(公告)号：US20100019155A1
公开(公告)日：2010-01-28
申请号：US11917947
申请日：2006-06-16
申请人： Derk Jan Wilfred Klunder , Maarten Marinus Johannes Wilhelm Van Herpen , Hendrik Roelof Stapert
发明人： Derk Jan Wilfred Klunder , Maarten Marinus Johannes Wilhelm Van Herpen , Hendrik Roelof Stapert
IPC分类号： G01N21/64 , G01N21/63
CPC分类号： G01N21/6428 , B82Y15/00 , G01N21/648 , G01N33/54373
摘要： The present invention provide a qualitative or quantitative luminescence sensor, for example a bio sensor or chemical sensor, using sub-wavelength aperture or slit structures, i.e. using apertures or slit structures having a smallest dimension smaller than the wavelength of the excitation radiation in the medium that fills the aperture or slit structure. The invention furthermore provides a method for the detection of luminescence radiation generated by one or more luminophores present in aperture or slit structure in such a luminescence sensor.
摘要翻译：本发明提供定性或定量发光传感器，例如使用亚波长孔径或狭缝结构的生物传感器或化学传感器，即使用具有小于介质中的激发辐射的波长的最小尺寸的孔或狭缝结构其填充孔或狭缝结构。本发明还提供了一种用于检测在这种发光传感器中存在于孔或狭缝结构中的一个或多个发光体产生的发光辐射的方法。

3. 发明授权

US10101321B2 Detecting target molecules in a sample 有权
公开(公告)号：US10101321B2
公开(公告)日：2018-10-16
申请号：US12446461
申请日：2007-10-19
申请人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： C40B30/10 , G01N33/53 , G01N21/64
摘要： The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate.

4. 发明申请

US20100197515A1 DETECTING TARGET MOLECULES IN A SAMPLE 审中-公开
标题翻译：在样品中检测目标分子
公开(公告)号：US20100197515A1
公开(公告)日：2010-08-05
申请号：US12446461
申请日：2007-10-19
申请人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhelm Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： C40B30/04 , C40B40/04
CPC分类号： G01N33/5306 , C40B30/10 , G01N21/6428
摘要： The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate.
摘要翻译：本发明涉及检测样品中靶分子的存在，其中样品与底物接触，随后在洗涤步骤中洗涤底物。特别地，本发明涉及检测样品中目标分子的存在的方法，该方法包括：（a）使样品（37）与其上固定有特异性结合靶分子的探针分子的底物接触; （b）通过洗涤液在洗涤步骤中洗涤基质（38）以去除或稀释未结合的靶分子; （c）检测所得结合复合物（39）在底物上的存在，以确定靶分子是否存在于样品中。洗涤液体基本上与基材的折射率匹配。

5. 发明申请

US20120040853A1 REAL TIME MULTIPLEX PCR DETECTION ON SOLID SURFACES USING DOUBLE STRANDED NUCLEIC ACID SPECIFIC DYES 审中-公开
标题翻译：使用双重棒状核酸特异性染料对固体表面进行实时多重PCR检测
公开(公告)号：US20120040853A1
公开(公告)日：2012-02-16
申请号：US13131333
申请日：2009-11-17
申请人： Anke Pierik , Derk Jan Wilfred Klunder , Marius Losif Boamfa , Richard Joseph Marinus Schroedes , Hendrik Roelof Stapert , Danielle Elisa Willemine Clout
发明人： Anke Pierik , Derk Jan Wilfred Klunder , Marius Losif Boamfa , Richard Joseph Marinus Schroedes , Hendrik Roelof Stapert , Danielle Elisa Willemine Clout
IPC分类号： C40B30/04
CPC分类号： C12Q1/6851 , C12Q2565/519 , C12Q2565/101 , C12Q2561/113
摘要： The present invention provides method allowing for real time detection of a multitude of target nucleic acids of interest in one reaction (multiplexing) using dyes that are specific for double stranded nucleic acids.
摘要翻译：本发明提供允许使用对双链核酸特异性的染料的一次反应（多重）中实时检测大量目标核酸的方法。

6. 发明申请

US20080272313A1 Luminescence Sensor Operating in Reflection Mode 有权
标题翻译：发光传感器在反射模式下工作
公开(公告)号：US20080272313A1
公开(公告)日：2008-11-06
申请号：US12158451
申请日：2006-12-19
申请人： Maarten Marinus Johannes Wilhemus Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
发明人： Maarten Marinus Johannes Wilhemus Van Herpen , Derk Jan Wilfred Klunder , Hendrik Roelof Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , G01N21/6428
摘要： The present invention relates to a luminescence sensor (10), such as a luminescence biosensor or a luminescence chemical sensor, comprising at least one aperture (1) having a first lateral dimension larger than the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with and a second lateral dimension below the diffraction limit of excitation radiation (3) in a medium the at least one aperture (1) is filled with. The invention furthermore relates to a method for the detection of luminescence radiation (5), e.g. fluorescence radiation, generated by at least one optically variable molecule (2), e.g. fluorophore, in the at least one aperture (1). The excitation radiation (3) is preferably polarised to suppress the excitation radiation (3) in the apertures (1). The luminescence sensor (10) according to the present invention is able to detect relatively low concentrations of optically variable molecules (2), e.g. fluorophores.
摘要翻译：本发明涉及一种发光传感器（10），例如发光生物传感器或发光化学传感器，其包括至少一个孔（1），其具有比介质中的激发辐射（3）的衍射极限大的第一横向尺寸所述至少一个孔（1）在所述至少一个孔（1）填充的介质中填充有低于激发辐射（3）的衍射极限的第二横向尺寸。本发明还涉及用于检测发光辐射（5）的方法，例如，由至少一个光学可变分子（2）产生的荧光辐射，例如。在所述至少一个孔（1）中的荧光团。激发辐射（3）优选地被极化以抑制孔（1）中的激发辐射（3）。根据本发明的发光传感器（10）能够检测相对低浓度的光学可变分子（2），例如，荧光团

7. 发明授权

US08815162B2 Microelectronic sensor device for DNA detection 有权
标题翻译：用于DNA检测的微电子传感器装置
公开(公告)号：US08815162B2
公开(公告)日：2014-08-26
申请号：US12303448
申请日：2007-05-24
申请人： Erik Robbert Vossenaar , Marc Wilhelmus Gijsbert Ponjee , Hendrik Roelof Stapert , Mark Thomas Johnson
发明人： Erik Robbert Vossenaar , Marc Wilhelmus Gijsbert Ponjee , Hendrik Roelof Stapert , Mark Thomas Johnson
IPC分类号： G05D23/00
CPC分类号： G01N21/6454 , B01L3/5027 , B01L3/50851 , B01L7/525 , B01L7/54 , B01L2200/147 , B01L2300/0636 , B01L2300/0819 , B01L2300/1822 , B01L2300/1827 , C12Q1/6825 , G01N21/0332 , G01N21/6428 , G01N2021/6439 , C12Q2565/1015 , C12Q2527/101 , C12Q2527/107
摘要： The invention relates to a microelectronic sensor device and a method for the investigation of biological target substances (20), for example oligonucleotides like DNA fragments. In one embodiment, the device comprises a reaction surface (RS) to which target specific reactants (10) are attached and which lies between a sample chamber (SC) and an array of selectively controllable heating elements (HE). The temperature profile in the sample chamber (SC) can be controlled as desired to provide for example conditions for a PCR and/or for a controlled melting of hybridizations. The reactant (10) and/or the target substance (20) comprises a label (12) with an observable property, like fluorescence, that changes if the target substance (20) is bound to the reactant (10), said property being detected by an array of sensor elements, for example photosensors (SE). The fluorescence of the label (12) may preferably be transferred by FRET to a different fluorescent label (22) or quenched if the target substance (20) is bound.
摘要翻译：本发明涉及一种微电子传感器装置和一种用于研究生物靶物质（20）的方法，例如DNA片段等寡核苷酸。在一个实施方案中，所述装置包括反应表面（RS），目标特异性反应物（10）附着到所述反应表面（RS），并且位于样品室（SC）和选择性可控制的加热元件（HE）阵列之间。样品室（SC）中的温度曲线可以根据需要进行控制，以提供例如PCR和/或受控熔化杂交的条件。反应物（10）和/或目标物质（20）包含具有可观察性质（如荧光）的标记物（12），如果目标物质（20）与反应物（10）结合，则其变化，所述性质被检测通过传感器元件阵列，例如光电传感器（SE）。标记物（12）的荧光优选通过FRET转移到不同的荧光标记物（22），或者如果靶物质（20）被结合则被淬灭。

8. 发明申请

US20110319275A1 METHOD FOR IMMOBILIZING NUCLEIC ACIDS ON A SUPPORT 有权
标题翻译：用于在支持物上固定核酸的方法
公开(公告)号：US20110319275A1
公开(公告)日：2011-12-29
申请号：US13121206
申请日：2009-09-22
申请人： Anke Pierik , Hendrik Roelof Stapert
发明人： Anke Pierik , Hendrik Roelof Stapert
IPC分类号： C40B20/08 , C40B50/18 , C40B40/06
CPC分类号： C12Q1/6837 , C12Q2525/173
摘要： The present invention relates to a method for immobilizing nucleic acids on a support, comprising the provision of a nucleic acid with a stretch of nucleotides of only one basetype and the immobilization of said nucleic acid on a support by crosslinking by light, wherein said crosslinking by light is performed at a wavelength of about 300-500 nm, preferably at a wavelength of 365 nm. The present invention further relates to a method for the analysis of nucleic acids immobilized according to the invention, which comprises the hybridization of the immobilized nucleic acid with complementary and mismatch segments. Furthermore, the present invention relates to immobilized nucleic acids obtainable by the method of the invention, the use of accordingly immobilized nucleic acids for the production of nucleic acid arrays and a diagnostic kit, comprising an array of nucleic acids which are immobilized according to the present invention.
摘要翻译：本发明涉及用于将核酸固定在载体上的方法，其包括提供具有仅一种碱基类型的核苷酸序列的核酸和通过光交联将所述核酸固定在载体上，其中所述交联通过光在约300-500nm的波长处进行，优选在365nm的波长下进行。本发明还涉及用于分析根据本发明固定的核酸的方法，其包括将固定的核酸与互补和错配片段杂交。此外，本发明涉及通过本发明的方法可获得的固定化核酸，使用相应地固定的核酸来生产核酸阵列和诊断试剂盒，其包含根据本发明固定的核酸阵列发明。

9. 发明授权

US07935213B2 Non-leaching adhesive system and its use in a liquid immersion objective 有权
标题翻译：非浸出粘合剂系统及其在液体浸入物中的应用
公开(公告)号：US07935213B2
公开(公告)日：2011-05-03
申请号：US12629155
申请日：2009-12-02
申请人： Emile Johannes Karel Verstegen , Johan George Kloosterboer , Hendrik Roelof Stapert , Jacobus Hermanus Maria Neijzen , Helmar van Santen
发明人： Emile Johannes Karel Verstegen , Johan George Kloosterboer , Hendrik Roelof Stapert , Jacobus Hermanus Maria Neijzen , Helmar van Santen
IPC分类号： B29C65/00 , B32B37/12 , B32B7/12
CPC分类号： C09J135/02
摘要： A non-leaching adhesive system and its use in a liquid immersion objective for immersion-writing of masters for optical discs are disclosed. The adhesive system comprises at least one monomer, selected from among the group of acrylate and methacrylate monomers, allylic monomers, norbornene monomers, hybrid monomers thereof, containing chemically different polymerizable groups, and multifunctional thiol monomers, provided that said thiol is used in combination with at least one of said non-thiol monomers; and a polymerization initiator. At least one of said monomers, not being a thiol, is provided with at least two functional polymerizable groups to obtain a crosslinked polymer network. The polymerization initiator is preferably an initiator that can be activated both thermally and with UV radiation. The adhesive system may further contain a reactive diluent. Further the use of the present adhesive system in mounting a liquid immersion objective is disclosed.
摘要翻译：公开了一种非浸出粘合剂系统及其在用于浸入式写入光盘的液浸物镜中的用途。粘合剂体系包含至少一种选自丙烯酸酯和甲基丙烯酸酯单体的单体，烯丙基单体，降冰片烯单体，含有化学不同的可聚合基团的杂化单体和多官能硫醇单体，条件是所述硫醇与所述非硫醇单体中的至少一种; 和聚合引发剂。至少一种所述单体（不是硫醇）具有至少两个官能的可聚合基团以获得交联的聚合物网络。聚合引发剂优选是能够以热辐射和UV辐射活化的引发剂。粘合剂体系还可以含有反应性稀释剂。此外，公开了本发明的粘合剂系统在安装液浸物镜中的用途。

10. 发明授权

US07642297B2 Non-leaching adhesive system and its use in a liquid immersion objective 有权
标题翻译：非浸出粘合剂系统及其在液体浸入物中的应用
公开(公告)号：US07642297B2
公开(公告)日：2010-01-05
申请号：US10568837
申请日：2004-08-05
申请人： Emile Johannes Karel Verstegen , Johan George Kloosterboer , Hendrik Roelof Stapert , Jacobus Hermanus Maria Neijzen , Helmar Van Santen
发明人： Emile Johannes Karel Verstegen , Johan George Kloosterboer , Hendrik Roelof Stapert , Jacobus Hermanus Maria Neijzen , Helmar Van Santen
IPC分类号： C08F2/46 , C09J4/02
CPC分类号： C09J135/02
摘要： A non-leaching adhesive system and its use in a liquid immersion objective for immersion-writing of masters for optical discs are disclosed. The adhesive system comprises at least one monomer, selected from among the group of acrylate and methacrylate monomers, allylic monomers, norbornene monomers, hybrid monomers thereof, containing chemically different polymeriazble groups, and multifunctional thiol monomers, provided that said thiol is used in combination with at least one of said non-thiol monomers; and a polymerization initiator. At least one of said monomers, not being a thiol, is provided with at least two functional polymerizable groups to obtain a crosslinked polymer network. The polymerization initiator is preferably an initiator that can be activated both thermally and with UV radiation. The adhesive system may further contain a reactive diluent. Further the use of the present adhesive system in mounting a liquid immersion objective is disclosed.
摘要翻译：公开了一种非浸出粘合剂系统及其在用于浸入式写入光盘的液浸物镜中的用途。粘合剂体系包含至少一种选自丙烯酸酯和甲基丙烯酸酯单体的单体，烯丙基单体，降冰片烯单体，含有化学上不同的聚合物基团的杂化单体和多官能硫醇单体，条件是所述硫醇与所述非硫醇单体中的至少一种; 和聚合引发剂。至少一种所述单体（不是硫醇）具有至少两个官能的可聚合基团以获得交联的聚合物网络。聚合引发剂优选是能够以热辐射和UV辐射活化的引发剂。粘合剂体系还可以含有反应性稀释剂。此外，公开了本发明的粘合剂系统在安装液浸物镜中的用途。

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