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    • 2. 发明授权
    • Grapevine fanleaf virus resistance in grapevine
    • 葡萄柚叶病毒抗性
    • US07211710B2
    • 2007-05-01
    • US10377435
    • 2003-02-28
    • Dennis GonsalvesBaodi XueTania KrastanovaKai-Shu LingMarc Fuchs
    • Dennis GonsalvesBaodi XueTania KrastanovaKai-Shu LingMarc Fuchs
    • C12N15/82C12N15/90
    • C07K14/005A01H1/04A01H5/08A01H6/88C12N15/8205C12N15/8283C12N2770/00022C12N2770/32022
    • In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease.
    • 通常,本发明的特征在于选择具有增加的对葡萄叶扇叶病抗性的转基因葡萄树或葡萄树成分的方法。 该方法包括:(a)在植物细胞中表达的葡萄籽叶病毒外壳蛋白核酸分子或其片段转化葡萄植物细胞; (b)从植物细胞再生转基因葡萄或葡萄成分; 和(c)选择表达葡萄叶扇叶病毒外壳蛋白或外壳蛋白片段的转基因葡萄或葡萄成分,其中外壳蛋白或其外壳蛋白片段以等于或小于约0.2的光密度的水平表达 在与对照植物相比通过酶联免疫测定法检测的405nm处,所选择的转基因葡萄树或葡萄树成分具有对葡萄叶扇叶病的抗性。