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1. 发明授权

US06667426B1 Grapevine fanleaf virus resistance in grapevine expressing grapevine fanleaf virus coat protein 失效
标题翻译：葡萄叶扇叶病毒外壳蛋白中葡萄叶扇叶病毒抗性
公开(公告)号：US06667426B1
公开(公告)日：2003-12-23
申请号：US09162017
申请日：1998-09-28
申请人： Dennis Gonsalves , Baodi Xue , Tania Krastanova , Kai-Shu Ling , Marc Fuchs
发明人： Dennis Gonsalves , Baodi Xue , Tania Krastanova , Kai-Shu Ling , Marc Fuchs
IPC分类号： C12N504
CPC分类号： C07K14/005 , A01H1/04 , A01H5/08 , A01H6/88 , C12N15/8205 , C12N15/8283 , C12N2770/00022 , C12N2770/32022
摘要： In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) transforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a ‘Geneva’ isolate of a grapevine nepovirus virus.
摘要翻译：通常，本发明的特征在于一种选择具有增加的扇叶病抗性的转基因葡萄树或葡萄树成分的方法，所述方法包括以下步骤：（a）用葡萄树突状病毒外壳蛋白核酸分子或片段转化葡萄植物细胞其能够在植物细胞中表达; （b）从植物细胞再生转基因葡萄或葡萄成分; 和（c）选择在低水平表达核酸分子或其片段的转基因葡萄或葡萄成分，其中低水平表达增加转基因葡萄或葡萄成分对扇叶病的抗性。本发明还涉及编码葡萄树病毒病毒的“日内瓦”分离物的外壳蛋白或其片段的分离的核酸分子。

2. 发明授权

US07211710B2 Grapevine fanleaf virus resistance in grapevine 有权
标题翻译：葡萄柚叶病毒抗性
公开(公告)号：US07211710B2
公开(公告)日：2007-05-01
申请号：US10377435
申请日：2003-02-28
申请人： Dennis Gonsalves , Baodi Xue , Tania Krastanova , Kai-Shu Ling , Marc Fuchs
发明人： Dennis Gonsalves , Baodi Xue , Tania Krastanova , Kai-Shu Ling , Marc Fuchs
IPC分类号： C12N15/82 , C12N15/90
CPC分类号： C07K14/005 , A01H1/04 , A01H5/08 , A01H6/88 , C12N15/8205 , C12N15/8283 , C12N2770/00022 , C12N2770/32022
摘要： In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease.
摘要翻译：通常，本发明的特征在于选择具有增加的对葡萄叶扇叶病抗性的转基因葡萄树或葡萄树成分的方法。该方法包括：（a）在植物细胞中表达的葡萄籽叶病毒外壳蛋白核酸分子或其片段转化葡萄植物细胞; （b）从植物细胞再生转基因葡萄或葡萄成分; 和（c）选择表达葡萄叶扇叶病毒外壳蛋白或外壳蛋白片段的转基因葡萄或葡萄成分，其中外壳蛋白或其外壳蛋白片段以等于或小于约0.2的光密度的水平表达在与对照植物相比通过酶联免疫测定法检测的405nm处，所选择的转基因葡萄树或葡萄树成分具有对葡萄叶扇叶病的抗性。

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