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1. 发明申请

US20160215254A1 SYSTEM AND METHOD FOR LASER LYSIS 审中-公开
标题翻译：激光雷达系统和方法
公开(公告)号：US20160215254A1
公开(公告)日：2016-07-28
申请号：US14909195
申请日：2014-09-23
申请人： Deirdre MELDRUM , Shih-Hui (Joseph) CHAO , Thai TRAN , Laimonas BAUSKAS , Jeff HOUKAL , Andrew HATCH , Weimin GAO , David Wayne RICHARDSON
发明人： Deirdre Meldrum , Shih-Hui (Joseph) Chao , Thai Tran , Laimonas Kelbauskas , Jeff Houkal , Andrew Hatch , Weimin Gao , David Richardson
IPC分类号： C12M1/00 , G01N1/28 , C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/0293 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M47/06 , C12Q1/6841 , C12Q1/6848 , C12Q1/686 , G01N1/286 , G01N2001/2886 , C12Q2547/101
摘要： The present invention provides a system and method for lysing individual cells in situ, including the steps of capturing a tissue sample comprising a cellular content, subjecting the tissue sample to a stream of continuous fluid flow, lysing a selected area of the tissue sample with a laser, thereby releasing at least a portion of the cellular content from the tissue sample, recovering at least one target molecule from the cellular content in the stream, and processing the at least one target molecule.
摘要翻译：本发明提供了一种用于原位裂解个体细胞的系统和方法，包括以下步骤：捕获包含细胞内容物的组织样品，使组织样品经受连续流体流动的流动，将组织样品的选定区域用激光，从而从组织样品中释放至少一部分细胞含量，从流中的细胞含量回收至少一种靶分子，以及处理该至少一种靶分子。

2. 发明申请

US20150133757A1 Endoscope for Analyte Consumption Rate Determination with Single Cell Resolution for In Vivo Applications 有权
标题翻译：用于体内应用的单细胞分辨率的分析物消耗率测定的内窥镜
公开(公告)号：US20150133757A1
公开(公告)日：2015-05-14
申请号：US14538557
申请日：2014-11-11
申请人： Deirdre Meldrum , Laimonas Kelbauskas , Jeff Houkal , Roger Johnson , Yanqing Tian , Cody Youngbull
发明人： Deirdre Meldrum , Laimonas Kelbauskas , Jeff Houkal , Roger Johnson , Yanqing Tian , Cody Youngbull
IPC分类号： A61B5/1459 , A61B1/07
CPC分类号： A61B5/1459 , A61B5/1455 , A61B5/6847
摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.
摘要翻译：包括使用光纤技术的改进的内窥镜和多个外在光学传感器的新型方法和装置，用于实现同时测定生物分析物在体内的单细胞分辨率的变化率。这些装置采用动态振荡致动来确定相对于电池的一个或多个方向的感兴趣的分析物的梯度。感兴趣的分析物的变化率或通量可以很容易地通过应用与流量和浓度梯度相关的第一个菲克定律来确定。器件的双重工作模式显着提高了测量灵敏度和精度。

3. 发明授权

US11045807B2 Integrated platform for characterization of single cells or small cell clusters 有权
公开(公告)号：US11045807B2
公开(公告)日：2021-06-29
申请号：US16479729
申请日：2018-02-26
申请人： Deirdre Meldrum , Laimonas Kelbauskas , Yanqing Tian , Honor Glenn , Clifford Anderson , Kristen Lee , Ganquan Song , Liqiang Zhang , Jeff Houkal , Fengyu Su , Benjamin Ueberroth , Jacob Messner , Hong Wang , Kimberly Bussey
发明人： Deirdre Meldrum , Laimonas Kelbauskas , Yanqing Tian , Honor Glenn , Clifford Anderson , Kristen Lee , Ganquan Song , Liqiang Zhang , Jeff Houkal , Fengyu Su , Benjamin Ueberroth , Jacob Messner , Hong Wang , Kimberly Bussey
IPC分类号： B01L3/00 , G01N15/14 , G02B21/34 , G01N15/10
摘要： An integrated technological platform enabling real-time quantitative multiparameter metabolic profiling, utilizing either or both of extra and intracellular optical sensors, individually or simultaneously. A scalable embedded micropocket array structure, generally fabricated on fused silica substrates, facilitates the integration of multiple, spatially separated extracellular sensors for multiparameter analysis in a container formed with the use of an activation mechanism forming part of a device configured to hold the container during the measurements. The creation of hermetically sealed microchambers is carried out with a pneumatically and/or mechanically and/or electromechanically driven device that is “floating” within the holding device and that is optionally equipped with a vacuum/suction mechanism to hold a component of the container at its surface.

4. 发明授权

US09597026B2 Endoscope for analyte consumption rate determination with single cell resolution for in vivo applications 有权
公开(公告)号：US09597026B2
公开(公告)日：2017-03-21
申请号：US14538557
申请日：2014-11-11
申请人： Deirdre Meldrum , Laimonas Kelbauskas , Jeff Houkal , Roger Johnson , Yanqing Tian , Cody Youngbull
发明人： Deirdre Meldrum , Laimonas Kelbauskas , Jeff Houkal , Roger Johnson , Yanqing Tian , Cody Youngbull
IPC分类号： A61B5/1455 , A61B5/1459 , A61B5/00
CPC分类号： A61B5/1459 , A61B5/1455 , A61B5/6847
摘要： Novel methods and devices including modified endoscopes that employ fiber optic technology and multiple extrinsic optical sensors for enablement of simultaneous determination of change rates of biological analytes in vivo with single-cell resolution. The devices employ dynamic oscillatory actuation to determine the gradient of analytes of interest along one or multiple directions with respect to the cell. The change rate or flux of the analyte of interest can be easily determined by applying the first Fick's law that relates the flux with the concentration gradient. The dual operation mode of the device markedly increases measurement sensitivity and accuracy.

5. 发明申请

US20150087007A1 MICROFLUIDIC ELECTROCAGE DEVICE AND CELL MEDIUM FOR TRAPPING AND ROTATING CELLS FOR LIVE-CELL COMPUTED TOMOGRAPHY (CT) 审中-公开
标题翻译：用于活细胞计算机图像（CT）的捕获和旋转细胞的微流控电极装置和细胞培养基
公开(公告)号：US20150087007A1
公开(公告)日：2015-03-26
申请号：US14497005
申请日：2014-09-25
申请人： Deirdre Meldrum , Roger Johnson , Iniyan Soundappa Elango , Andrew Shabilla , Hong Wang , Jakrey Myers , Laimonas Kelbauskas , Dean Smith , Pimwadee Limsirichai
发明人： Deirdre Meldrum , Roger Johnson , Iniyan Soundappa Elango , Andrew Shabilla , Hong Wang , Jakrey Myers , Laimonas Kelbauskas , Dean Smith , Pimwadee Limsirichai
IPC分类号： G01N33/50 , B01L3/00 , C23F1/30
CPC分类号： B01L3/502707 , B01L3/502761 , B01L2300/0645 , C12M23/16 , C12M41/36 , C23F1/02 , C23F1/30 , C23F1/44
摘要： A microfluidic device useable for performing live cell computed tomography imaging is fabricated with a cover portion including a first wafer with at least one metal patterned thereon, a base portion including a second wafer with at least one metal patterned thereon and negative photoresist defining recesses therein, and a diffusive bonding layer including a negative photoresist arranged to join the cover portion and the base portion. A composition useful in live cell computer topography includes a long-chain polysaccharide at a concentration of from about 0.01% to about 10.0% in cell culture medium for supporting cell life while enabling cell rotation rate to be slowed to a speed commensurate with low light level imaging.
摘要翻译：用于执行活细胞计算机断层摄影成像的微流体装置制造成具有覆盖部分，所述覆盖部分包括具有图案化的至少一个金属的第一晶片，包括至少一个图案化的金属的第二晶片的基部和其中限定凹部的负光致抗蚀剂，以及包括负极光致抗蚀剂的漫射粘结层，所述负性光致抗蚀剂被布置成接合盖部分和基部。用于活细胞计算机地形的组合物包括在细胞培养基中浓度为约0.01％至约10.0％的长链多糖，用于支持细胞寿命，同时使细胞旋转速率降低至与低光级相当的速度成像。

6. 发明申请

US20050070944A1 Method and apparatus for isolating cell nuclei from biopsy obtained tissue 审中-公开
标题翻译：用于从活组织检查获得的组织中分离细胞核的方法和装置
公开(公告)号：US20050070944A1
公开(公告)日：2005-03-31
申请号：US10847950
申请日：2004-05-14
申请人： Mark Holl , Andrew Miller , Matthew Stanton , Carissa Sanchez , Thomas Paulson , Brian Reid , Deirdre Meldrum
发明人： Mark Holl , Andrew Miller , Matthew Stanton , Carissa Sanchez , Thomas Paulson , Brian Reid , Deirdre Meldrum
IPC分类号： A61B17/14 , C12M3/08
CPC分类号： C12M47/06
摘要： A rotor (14) is located within a rotor chamber (12) formed in a housing (24). A drive shaft (16) extends from a motor and couples to the drive shaft interconnect (46) at the center of the rotor (14). One side of the rotor (14) is provided with circumferentially spaced apart radial rows of mincing edges (20). These edges (20) are situated on concentric circles or on a spiral. They are elongated circumferentially and may be situated substantially on an arc and substantially on an arc or on a chord. During use, the rotor (14) is rotated after biopsy obtained tissue (10) has been placed in the rotor chamber (12) on the side of the rotor (14) that includes the mincing edges (20). A fluid is delivered into and out from the rotor chamber (12) as the rotor (14) is being rotated to mince the biopsy, lyse the cells, and release the cell nuclei from the obtained tissue. Cell nuclei are expelled from the chamber (12) and are moved through a filter (58). The cell nuclei are collected and then delivered to a flow cytometry apparatus to be analyzed.
摘要翻译：转子（14）位于形成在壳体（24）内的转子室（12）内。驱动轴（16）从马达延伸并在转子（14）的中心处联接到驱动轴互连（46）。转子（14）的一侧设置有周向间隔开的排列的切割边缘（20）。这些边缘（20）位于同心圆或螺旋上。它们沿周向延伸并且可以基本上位于弧形上并且基本上位于弧形或弦上。在使用过程中，在活组织检查后，转子（14）在组装（10）被放置在转子室（12）的转子（14）的包括切碎边缘（20）的侧面上后旋转。当转子（14）旋转时，将流体输送到转子室（12）中并使其流出，从而破坏活组织检查，裂解细胞，并从获得的组织释放细胞核。细胞核从腔室（12）排出并移动通过过滤器（58）。收集细胞核，然后送至流式细胞术仪器进行分析。

7. 发明授权

US11332782B2 Method for detecting nucleotide polymorphisms 有权
公开(公告)号：US11332782B2
公开(公告)日：2022-05-17
申请号：US16323397
申请日：2017-08-07
申请人： Rhett Martineau , Deirdre Meldrum
发明人： Rhett Martineau , Deirdre Meldrum
IPC分类号： C12Q1/6858 , C12Q1/6883 , C12N9/12 , C12N15/09
摘要： Methods for detecting single nucleotide polymorphisms in nucleotide sequences using LAMP reactions are provided herein. Generally, two sets of LAMP primers, a wild-type primer that matches expected DNA sequences and an SNP primer that matches the expected SNP DNA are provided. One method includes providing the wild-type primer and the SNP primer in separate wells of a multi-well microfluidic array device, adding the sample nucleotide sequence into the wells seeded with the primers, and initiating LAMP reactions within the wells. The method includes observing the reaction differential between the primers and determining the status of the DNA with regard to that particular SNP. A second method includes providing the primers with tags in a mixture, adding the sample nucleotide sequence to the mixture, and initiating LAMP reactions. The method includes providing a different visual indication when the wild-type primer reacts with the sample nucleotide sequence versus when the SNP primer reacts with the sample nucleotide sequence, and determining the status of the DNA with regard to that particular SNP.

8. 发明授权

US09944955B1 Photosynthetic production of 3-hydroxybutyrate from carbon dioxide 有权
公开(公告)号：US09944955B1
公开(公告)日：2018-04-17
申请号：US14479893
申请日：2014-09-08
申请人： Bo Wang , Weiwen Zhang , Deirdre Meldrum , David Nielsen
发明人： Bo Wang , Weiwen Zhang , Deirdre Meldrum , David Nielsen
IPC分类号： C12P7/52 , C12N9/04 , C12N9/10 , C12N9/16 , C07C59/01
CPC分类号： C12P7/52 , C07C59/01 , C12N9/0006 , C12N9/1029 , C12N9/16 , C12Y101/01036 , C12Y101/01157 , C12Y203/01009 , C12Y301/0202
摘要： Construction and expression of synthetic pathways to produce (5) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.

9. 发明授权

US09181375B2 Fluorescent potassium ion sensors 有权
标题翻译：荧光钾离子传感器
公开(公告)号：US09181375B2
公开(公告)日：2015-11-10
申请号：US13985260
申请日：2012-02-13
申请人： Yanqing Tian , Deirdre Meldrum , Xianfeng Zhou , Fengyu Su , Roger H. Johnson , Cody Youngbull
发明人： Yanqing Tian , Deirdre Meldrum , Xianfeng Zhou , Fengyu Su , Roger H. Johnson , Cody Youngbull
IPC分类号： C08F226/06 , C07D498/08 , C07D519/00 , G01N33/84 , C07F15/00 , C08F230/04
CPC分类号： C08F226/06 , C07D498/08 , C07D519/00 , C07F15/0093 , C08F230/04 , C08G2261/226 , C08G2261/3223 , G01N33/84
摘要： This disclosure relates generally to potassium ion sensors and monomers derived from such sensors. The disclosure also provides for polymers (e.g., random copolymers, nanoparticles, polymer thin films, and sensors) having polymerized monomeric potassium ion sensors as described herein. These compounds and polymers are useful for measuring intracellular and extracellular potassium ion concentrations.
摘要翻译：本公开一般涉及衍生自这种传感器的钾离子传感器和单体。本公开还提供了具有如本文所述的聚合的单体钾离子传感器的聚合物（例如，无规共聚物，纳米颗粒，聚合物薄膜和传感器）。这些化合物和聚合物可用于测量细胞内和细胞外钾离子浓度。

10. 发明申请

US20150126404A1 CELLARIUM: THIN-FILM SENSOR WITH MICROARRAY SEAL 有权
标题翻译：细胞膜：微型密封薄膜传感器
公开(公告)号：US20150126404A1
公开(公告)日：2015-05-07
申请号：US14533349
申请日：2014-11-05
申请人： Yanqing Tian , Liqiang Zhang , Fengyu Su , Deirdre Meldrum , Sean Buizer , Clifford L. Anderson , Laimonas Kelbauskas , Kristen Lee
发明人： Yanqing Tian , Liqiang Zhang , Fengyu Su , Deirdre Meldrum , Sean Buizer , Clifford L. Anderson , Laimonas Kelbauskas , Kristen Lee
IPC分类号： G01N33/50 , G01N21/64 , G01N33/84
CPC分类号： G01N33/84 , G01N21/6428 , G01N33/66
摘要： A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.
摘要翻译：三重传感器，用于同时测量葡萄糖，氧气和pH值。传感器组件处于薄膜状态，例如感测膜或膜，葡萄糖探针与光谱蓝色部分中的辐射相关，与红色光谱中的辐射相关的氧探针，以及pH探针 - 具有绿色部分的光谱。光学探针被化学接枝或固定在合适的聚合物基质中，减轻了探针从基体中的浸出，改善了薄膜感测的稳定性，并使得能够重复使用相同的传感膜。

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