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1. 发明授权

US6054278A Ribosomal RNA gene polymorphism based microorganism identification 失效
标题翻译：基于核糖体RNA基因多态性的微生物鉴定
公开(公告)号：US6054278A
公开(公告)日：2000-04-25
申请号：US73465
申请日：1998-05-05
申请人： Deborah E. Dodge , Douglas H. Smith
发明人： Deborah E. Dodge , Douglas H. Smith
IPC分类号： C12Q1/68 , C12N25/11
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： The invention relates the discovery that the polymorphisms in 16S rRNA genes may be used to identify unknown bacterial isolates at the level of species, subspecies and strain identification. The invention also relates to the discovery that the sequences of individual are required. The invention involves the generation of composite polynucleotide sequence of the 16S rRNA genes of a microorganism of interest. The invention is to provide methods for identifying microorganisms. The methods comprise the steps of generating a composite 16S rRNA region sequence from a microorganism of interest. The composite 16S rRNA region sequence reveals polymorphisms within the 16S ribosomal RNA gene of the microorganism. The composite 16S rRNA region sequence may then be compared with a list of previously obtained composite 16S rRNA region sequences from reference microorganisms so as to determine the species, subspecies, or strain of the microorganism of interest. Another aspect of the invention is to provide computer based methods of identifying a microorganism.
摘要翻译：本发明涉及16S rRNA基因多态性可用于鉴定物种，亚种和菌株鉴定水平的未知细菌分离物的发现。本发明还涉及要求个体序列的发现。本发明涉及产生感兴趣的微生物的16S rRNA基因的复合多核苷酸序列。本发明提供鉴定微生物的方法。所述方法包括从感兴趣的微生物产生复合16S rRNA区序列的步骤。复合16S rRNA区序列揭示微生物16S核糖体RNA基因内的多态性。然后将复合16S rRNA区序列与来自参照微生物的先前获得的复合16S rRNA区序列的列表进行比较，以确定感兴趣的微生物的物种，亚种或菌株。本发明的另一方面是提供基于计算机的识别微生物的方法。

2. 发明授权

US5998143A Cycle sequencing thermal profiles 失效
公开(公告)号：US5998143A
公开(公告)日：1999-12-07
申请号：US986176
申请日：1997-12-05
申请人： Nicole M. Ellis , Deborah E. Dodge , Douglas H. Smith
发明人： Nicole M. Ellis , Deborah E. Dodge , Douglas H. Smith
IPC分类号： C12M1/00 , B01L7/00 , C12M1/36 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6869
摘要： The present invention relates to improved methods of generating polynucleotide sequencing reaction products from cycle sequencing and relates to various instruments and reagents for use in the subject methods. The use of "step-down" thermal profiles in conjunction with cycle sequencing is described. Step-down thermal profiles are formed by combining several thermal cycle sets such that the annealing temperature of each thermal cycle set is less than the annealing temperature of the preceding thermal cycle set. One embodiment of the invention is a method of generating a plurality of polynucleotide sequencing reaction products in parallel by subjecting a plurality of sequencing solution preparations to a step-down thermal profile, i.e., exposure to repeated thermal cycle sets, each thermal cycle set having an annealing temperature that is lower than the annealing temperature of the annealing phases of the preceding thermal cycle set. Other embodiments of the invention include systems for generating a plurality of polynucleotide sequencing reaction products in parallel. The systems comprise (i) a programmable thermal cycler programmed to perform a step-down thermal profile and (ii) a plurality of polynucleotide sequencing preparations. Additional embodiments of the invention include sets of polynucleotide sequencing reaction preparations that may be used in the methods of the invention.Another aspect of the invention is a device for generating a plurality of polynucleotide sequencing reaction products. The devices of the invention include (i) a plurality of polynucleotide sequencing reaction preparation chambers, each chamber containing a sequencing primer, (ii) a common fluid entry port, and (iii) a fluid dispensing channel, the channel connecting each of the sequencing reaction preparation chambers to the common fluid entry port. At least two of the sequencing primers in the device have Tms different from one another by at least 2.degree. C.

3. 发明授权

US08790666B2 Nerve construct containing living stretch-grown nervous tissue 有权
标题翻译：神经构造包含活的拉伸生长的神经组织
公开(公告)号：US08790666B2
公开(公告)日：2014-07-29
申请号：US11429201
申请日：2006-05-05
申请人： Douglas H. Smith
发明人： Douglas H. Smith
IPC分类号： A61K9/00
CPC分类号： A61N1/04 , A61N1/05 , A61N1/326 , A61N1/36103
摘要： The present invention relates to mechanically elongated neurons and provides useful compositions, devices and methods for treating a nerve lesion using such mechanically elongated neurons.
摘要翻译：本发明涉及机械伸长的神经元，并且提供使用这种机械拉伸的神经元治疗神经损伤的有用的组合物，装置和方法。

4. 发明授权

US06702474B2 Component assembly with formed spindle end portion 有权
公开(公告)号：US06702474B2
公开(公告)日：2004-03-09
申请号：US10195025
申请日：2002-07-11
申请人： Alan Charles Webb , Peter Holland , Peter Standring , Douglas H. Smith , Larry L. Bahney
发明人： Alan Charles Webb , Peter Holland , Peter Standring , Douglas H. Smith , Larry L. Bahney
IPC分类号： F16C1938
CPC分类号： B60B27/001 , B23P11/005 , B60B35/18 , B60B2310/208 , B60B2310/228 , B60B2310/306 , B60B2310/316 , B60B2360/10 , B60B2360/102 , B60B2380/14 , F16C19/386 , F16C35/063 , F16C2326/02 , Y10T29/49533 , Y10T29/49535 , Y10T29/49536 , Y10T29/49682 , Y10T29/49696 , Y10T29/49776 , Y10T29/49918 , Y10T29/53065 , Y10T29/53104
摘要： A hub (2) includes a spindle (14) which projects through a housing (4) and rotates relative to the housing (4) on a bearing (6) that is located between the spindle (14) and the housing (4). The bearing (6) has two sets of raceways (28,40) that are oblique to the axis x, and in addition rolling elements (36) arranged in two rows between the sets of raceways (28,40). The inner raceways (28) that fit around the spindle (14) and have back faces (32), with the back face (32) for one of the races (26) being against a shoulder (18) from which the spindle (14) projects. Initially, the end of the spindle (14) projects straight beyond the back face (32) of the outer race—indeed, so that the races (26) can be installed over the spindle (14). But once the housing (4) and races (26) are in a position around the spindle (14), the projecting end portion of the spindle (14) is deformed radially and axially in a rotary forming operation such that it transforms into a formed end (20) that lies behind back face (32) of the race (26). With the two races (26) between the formed end (20) and the shoulder (18). During the rotary forming operation the hub (26) rotates, and the end of its spindle (14) is forced against a rotating forming tool and the force is monitored. The housing (4) is restrained and the torque transferred from the rotating hub (2) to the housing (4) is monitored.

5. 发明授权

US06443622B1 Spindle and component assembly with formed spindle end portion 有权
标题翻译：主轴和部件组装，带有成型主轴端部
公开(公告)号：US06443622B1
公开(公告)日：2002-09-03
申请号：US09446671
申请日：1999-12-23
申请人： Alan Charles Webb , Peter Holland , Peter Standring , Douglas H. Smith , Larry L. Bahney
发明人： Alan Charles Webb , Peter Holland , Peter Standring , Douglas H. Smith , Larry L. Bahney
IPC分类号： F16C1938
CPC分类号： B23P11/005 , B23P11/00 , B60B27/001 , B60B35/18 , B60B2310/208 , B60B2310/228 , B60B2310/306 , B60B2310/316 , B60B2360/10 , B60B2360/102 , B60B2380/14 , F16C19/386 , F16C35/063 , F16C41/007 , F16C2326/02 , G01P3/443 , G01P3/446
摘要： A hub (2) includes a spindle (14) which projects through a housing (4) and rotates relative to the housing (4) on a bearing (6) that is located between the spindle (14) and the housing (4). The bearing (6) has two sets of raceways (28, 40) that are oblique to the axis x, and in addition rolling elements (36) arranged in two rows between the sets of raceways (28, 40). The inner raceways (28) that fit around the spindle (14) and have back faces (32), with the back face (32) for one of the races (26) being against a shoulder (18) from which the spindle (14) projects. Initially, the end of the spindle (14) projects straight beyond the back face (32) of the outer race—indeed, so that the races (26) can be installed over the spindle (14). But once the housing (4) and races (26) are in a position around the spindle (14), the projecting end portion of the spindle (14) is deformed radially and axially in a rotary forming operation such that it transforms into a formed end (20) that lies behind back face (32) of the race (26). With the two races (26) between the formed end (20) and the shoulder (18). During the rotary forming operation the hub (26) rotates, and the end of its spindle (14) is forced against a rotating forming tool and the force is monitored. The housing (4) is restrained and the torque transferred from the rotating hub (2) to the housing (4) is monitored.
摘要翻译：轮毂（2）包括主轴（14），该主轴突出通过壳体（4）并且相对于壳体（4）在位于主轴（14）和壳体（4）之间的轴承（6）上旋转。轴承（6）具有与轴线x倾斜的两组滚道（28,40），另外还有两组排列在滚道组（28,40）之间的滚动元件（36）。安装在主轴（14）周围并且具有后表面（32）的内滚道（28），其中一个座圈（26）的后表面（32）抵靠在肩部（18）上，主轴）项目。最初，主轴（14）的端部直接突出超过外圈的背面（32），实际上，使得座圈（26）可以安装在主轴（14）上。但是，一旦壳体（4）和座圈（26）处于围绕心轴（14）的位置，则主轴（14）的突出端部在旋转成形操作中径向和轴向地变形，使得其转变成形成端部（20）位于座圈（26）的背面（32）之后。通过形成的端部（20）和肩部（18）之间的两个座圈（26）。在旋转成形操作期间，毂（26）旋转，并且其主轴（14）的端部被迫抵抗旋转成形工具，并且该力被监测。对壳体（4）进行限制，并且监测从旋转轮毂（2）传递到壳体（4）的扭矩。

6. 发明授权

US5951015A Interactive arcade game apparatus 失效
标题翻译：互动街机游戏机
公开(公告)号：US5951015A
公开(公告)日：1999-09-14
申请号：US90023
申请日：1998-06-10
申请人： Douglas H. Smith , James E. Stoneham , William P. Fricke , Anthony L. Creed , Peter N. Skillman , Johnson K. Chow
发明人： Douglas H. Smith , James E. Stoneham , William P. Fricke , Anthony L. Creed , Peter N. Skillman , Johnson K. Chow
IPC分类号： F41N9/14
CPC分类号： F41J5/052 , A63B24/0006 , A63B24/0075 , A63B63/00 , A63B69/002 , A63B71/0605 , F41J5/24 , A63B2071/0694 , A63B2220/40 , A63B2220/805 , A63B2220/806 , A63B2220/807 , A63B2220/833 , A63B2225/50 , A63B24/0084 , A63B69/36
摘要： An interactive game apparatus in which players throw projectiles, such as soft balls, against a display panel on which a target image is projected and contact sensitive elements aligned with target areas in the projected image are activated by a hit from the thrown projectile to initiate a change in the projected image uniquely associated with the target area hit and the player who threw the projectile. An embodiment in the context of a tic-tac-toe game is described. A number of embodiments for identifying specific projectiles with a player responsible for throwing the projectile are described.
摘要翻译：一种交互式游戏装置，其中玩家将诸如软球的抛射物投射到其上投射有目标图像的显示面板上并且与投影图像中的目标区域对准的接触敏感元件被来自抛出的射弹的命中激活以启动改变与目标区域击中唯一相关联的投影图像和投掷射弹的玩家。描述了在tic-tac-toe游戏的上下文中的一个实施例。描述了用负责投掷弹药的玩家识别特定射弹的多个实施例。

7. 发明申请

US20100073678A1 Blast injury dosimeter 有权
标题翻译：爆炸剂量计
公开(公告)号：US20100073678A1
公开(公告)日：2010-03-25
申请号：US12383777
申请日：2009-03-27
申请人： Douglas H. Smith , Shu Yang , D. Kacy Cullen
发明人： Douglas H. Smith , Shu Yang , D. Kacy Cullen
IPC分类号： G01J3/46 , G01N21/55
CPC分类号： G01P15/06
摘要： A method for detecting blast induced pressure changes includes exposing a material, a contained solution, a membrane-bound solution, or a photonic crystal material, having a first optical property, to a pressure wave having a blast level. A change in the first optical property to a second optical property of the exposed material, contained solution, membrane-bound solution, or photonic crystal material is determined, and the extent of change corresponds to the blast level.
摘要翻译：用于检测炸药诱发的压力变化的方法包括将具有第一光学特性的材料，所含溶液，膜结合溶液或光子晶体材料暴露于具有爆炸级别的压力波。确定曝光材料，包含溶液，膜结合溶液或光子晶体材料的第一光学特性与第二光学特性的变化，并且变化程度对应于爆破级别。

8. 发明授权

US07045287B2 Method for contacting fluid components with moieties on a surface 失效
公开(公告)号：US07045287B2
公开(公告)日：2006-05-16
申请号：US09792169
申请日：2001-02-21
申请人： Douglas H. Smith , Gary B. Gordon
发明人： Douglas H. Smith , Gary B. Gordon
IPC分类号： C12Q1/68 , C12M3/00 , G01N33/566 , C07K1/00 , C07H21/02
CPC分类号： G01N33/54306 , B01L3/5021 , B01L3/50273 , B01L2300/046 , B01L2300/0636 , B01L2400/0409 , C12Q1/6813 , G01N33/54353 , Y10T436/143333
摘要： An improved method is provided for conducting chemical or biochemical reactions on a substrate surface in which the reactions take place in an enclosed chamber between components of a fluid and molecular moieties present on an interior surface of the chamber, wherein the improvement involves mixing the fluid during the reactions and maximizing contact between the fluid's components and the entirety of the reactive interior surface by (a) applying centrifugal force to the chamber and simultaneously (b) rotating the chamber about an axis thereof. The improved method is particularly advantageous in the context of a hybridization assay in which molecular components within a sample fluid hybridize to surface-bound molecular probes (e.g., as may be present in a spatially defined and physically addressable array) within a hybridization chamber, and wherein process and device parameters (e.g., sample volume, chamber volume, temperature, number of parts and materials, and the like) are critical. The invention enables use of a very small sample volume without need for a correspondingly small device, minimizes contamination (device components are disposable and the number of parts with which a fluid comes into contact is minimal), and further enables heating of the device prior to contacting the reactive surface with a sample fluid.

9. 发明授权

US5270839A Dual imaging station photoprint scanner 失效
标题翻译：双成像站照相打印扫描仪
公开(公告)号：US5270839A
公开(公告)日：1993-12-14
申请号：US762323
申请日：1991-09-16
申请人： Kenneth A. Parulski , Douglas H. Smith , Richard F. Manley , Carole A. Bilson
发明人： Kenneth A. Parulski , Douglas H. Smith , Richard F. Manley , Carole A. Bilson
IPC分类号： H04N1/04 , H04N1/195 , H04N1/393 , H04N5/253 , H04N1/40
CPC分类号： H04N1/393 , H04N1/195 , H04N1/0464 , H04N2201/0434
摘要： A dual image station photoprint scanner comprises a cabinet having an upper, album imaging station and a lower, magazine-fed, platen imaging station. The upper imaging station includes a translatable unit having a glass plate upon which a photoprint is placed in a face- down position. Adjacent to the top plate, the translatable cabinet has a sloped support surface, to accommodate a photo album. A lower portion of the cabinet contains a platen feed mechanism, which translates a photographic print support platen from a supply magazine to a platen imaging station, and then feeds the platen to a storage magazine. A high resolution CCD image sensor is supported at an image projection plane of a multidirectional image projection mechanism. A digital imagery data recording mechanism is coupled to the image sensor and is operative to record, on a digital data storage medium, a digitized image that has been projected on the image sensor. The multi-directional image projection mechanism comprises an adjustable image magnification device, such as an adjustable focal length, adjustable focus, zoom lens, which is operative to controllably vary the size of the image projected onto the image sensor in accordance with the contents of one or more photoprint parameter regions on the platen. The image projection mechanism also includes a controllably rotatable mirror disposed between the top plate and the platen imaging station for selectively providing an optical projection path from one of the imaging stations to the CCD sensor.

10. 发明授权

US3986011A Printer-plotter system 失效
标题翻译：打印机绘图仪系统
公开(公告)号：US3986011A
公开(公告)日：1976-10-12
申请号：US552094
申请日：1975-02-24
申请人： John S. Poole , H. Blair Muhlestein , Douglas H. Smith , Lennart Bilen
发明人： John S. Poole , H. Blair Muhlestein , Douglas H. Smith , Lennart Bilen
IPC分类号： G01D9/08 , G01D15/10 , G06F3/13 , G06K15/22 , G06F3/12 , G06K15/02
CPC分类号： G06F3/13 , G01D15/10 , G06K15/22
摘要： A gas chromatograph system includes a central processor (CPU) and a printer-plotter. The printer-plotter prints characters and plots data using a single 1 by 7 array of thermal resistors to perform both the printing and plotting operations in real time. The system provides continuous and uniform traces despite changes in the plotting rate.Data from the chromatograph is temporarily stored by the CPU, then forwarded upon programmed command through a print-plot controller to the printer-plotter. Data is plotted as it is received from the CPU. As peaks in the data are detected, plotting is suspended temporarily without loss of data, and characters are printed adjacent to the peaks, thus annotating the peaks with digital data.
摘要翻译：气相色谱系统包括中央处理器（CPU）和打印机绘图仪。打印机绘图仪使用单个1/7个热敏电阻阵列打印字符并绘制数据，以实时执行打印和绘图操作。尽管绘图速率有变化，系统仍提供连续和均匀的轨迹。

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