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1. 发明授权

US6054278A Ribosomal RNA gene polymorphism based microorganism identification 失效
标题翻译：基于核糖体RNA基因多态性的微生物鉴定
公开(公告)号：US6054278A
公开(公告)日：2000-04-25
申请号：US73465
申请日：1998-05-05
申请人： Deborah E. Dodge , Douglas H. Smith
发明人： Deborah E. Dodge , Douglas H. Smith
IPC分类号： C12Q1/68 , C12N25/11
CPC分类号： C12Q1/689 , C12Q2600/156
摘要： The invention relates the discovery that the polymorphisms in 16S rRNA genes may be used to identify unknown bacterial isolates at the level of species, subspecies and strain identification. The invention also relates to the discovery that the sequences of individual are required. The invention involves the generation of composite polynucleotide sequence of the 16S rRNA genes of a microorganism of interest. The invention is to provide methods for identifying microorganisms. The methods comprise the steps of generating a composite 16S rRNA region sequence from a microorganism of interest. The composite 16S rRNA region sequence reveals polymorphisms within the 16S ribosomal RNA gene of the microorganism. The composite 16S rRNA region sequence may then be compared with a list of previously obtained composite 16S rRNA region sequences from reference microorganisms so as to determine the species, subspecies, or strain of the microorganism of interest. Another aspect of the invention is to provide computer based methods of identifying a microorganism.
摘要翻译：本发明涉及16S rRNA基因多态性可用于鉴定物种，亚种和菌株鉴定水平的未知细菌分离物的发现。本发明还涉及要求个体序列的发现。本发明涉及产生感兴趣的微生物的16S rRNA基因的复合多核苷酸序列。本发明提供鉴定微生物的方法。所述方法包括从感兴趣的微生物产生复合16S rRNA区序列的步骤。复合16S rRNA区序列揭示微生物16S核糖体RNA基因内的多态性。然后将复合16S rRNA区序列与来自参照微生物的先前获得的复合16S rRNA区序列的列表进行比较，以确定感兴趣的微生物的物种，亚种或菌株。本发明的另一方面是提供基于计算机的识别微生物的方法。

2. 发明授权

US5998143A Cycle sequencing thermal profiles 失效
公开(公告)号：US5998143A
公开(公告)日：1999-12-07
申请号：US986176
申请日：1997-12-05
申请人： Nicole M. Ellis , Deborah E. Dodge , Douglas H. Smith
发明人： Nicole M. Ellis , Deborah E. Dodge , Douglas H. Smith
IPC分类号： C12M1/00 , B01L7/00 , C12M1/36 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6869
摘要： The present invention relates to improved methods of generating polynucleotide sequencing reaction products from cycle sequencing and relates to various instruments and reagents for use in the subject methods. The use of "step-down" thermal profiles in conjunction with cycle sequencing is described. Step-down thermal profiles are formed by combining several thermal cycle sets such that the annealing temperature of each thermal cycle set is less than the annealing temperature of the preceding thermal cycle set. One embodiment of the invention is a method of generating a plurality of polynucleotide sequencing reaction products in parallel by subjecting a plurality of sequencing solution preparations to a step-down thermal profile, i.e., exposure to repeated thermal cycle sets, each thermal cycle set having an annealing temperature that is lower than the annealing temperature of the annealing phases of the preceding thermal cycle set. Other embodiments of the invention include systems for generating a plurality of polynucleotide sequencing reaction products in parallel. The systems comprise (i) a programmable thermal cycler programmed to perform a step-down thermal profile and (ii) a plurality of polynucleotide sequencing preparations. Additional embodiments of the invention include sets of polynucleotide sequencing reaction preparations that may be used in the methods of the invention.Another aspect of the invention is a device for generating a plurality of polynucleotide sequencing reaction products. The devices of the invention include (i) a plurality of polynucleotide sequencing reaction preparation chambers, each chamber containing a sequencing primer, (ii) a common fluid entry port, and (iii) a fluid dispensing channel, the channel connecting each of the sequencing reaction preparation chambers to the common fluid entry port. At least two of the sequencing primers in the device have Tms different from one another by at least 2.degree. C.

3. 发明授权

US5912117A Method for diagnosis of lyme disease 失效
标题翻译：淋巴系疾病诊断方法
公开(公告)号：US5912117A
公开(公告)日：1999-06-15
申请号：US923871
申请日：1992-10-09
申请人： Deborah E. Dodge , Thomas J. White
发明人： Deborah E. Dodge , Thomas J. White
IPC分类号： C12Q1/04 , C12Q1/68 , C12R1/01 , C23C14/54
CPC分类号： C12Q1/689 , C12Q1/6806 , Y10S435/81
摘要： Methods and reagents are provided for amplifying and detecting target nucleic acid sequences in a sample suspected of containing Borrelia spirochetes using the polymerase chain reaction (PCR) process. The PCR process employs primers and probes which are derived from the 16S ribosomal RNA gene of Borrelia burgdorferi and Borrelia hermsii.
摘要翻译： PCT No.PCT / US91 / 01574 Sec。 371日期：1992年10月9日 102（e）日期1992年10月9日PCT 1991年3月7日PCT公布。 WO91 / 14002 PCT出版物日期1991年9月19日提供了使用聚合酶链反应（PCR）方法来扩增和检测疑似含有螺旋体螺旋体的样品中的靶核酸序列的方法和试剂。 PCR过程采用引物和探针，其来源于布氏疏螺旋体和疏螺旋体的16S核糖体RNA基因。

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