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1. 发明授权

US06586193B2 Analyte assay using particulate labels 失效
公开(公告)号：US06586193B2
公开(公告)日：2003-07-01
申请号：US08953713
申请日：1997-10-17
申请人： Juan Yguerabide , Evangelina E. Yguerabide , David E. Kohne , Jeffrey T. Jackson
发明人： Juan Yguerabide , Evangelina E. Yguerabide , David E. Kohne , Jeffrey T. Jackson
IPC分类号： C12Q168
CPC分类号： G01N33/54313 , C12Q1/6816 , C12Q1/6834 , G01N15/14 , G01N15/1425 , G01N15/1459 , G01N33/585 , G01N2015/0038 , G01N2015/1493 , Y10S436/805 , Y10S436/817 , C12Q2563/103
摘要： Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

2. 发明授权

US5612183A Method for determining the effect of antimicrobial agents on growth using ribosomal nucleic acid subunit subsequence specific probes 失效
标题翻译：使用核糖体核酸亚基亚序列特异性探针测定抗微生物剂对生长的影响的方法
公开(公告)号：US5612183A
公开(公告)日：1997-03-18
申请号：US460531
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/18 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category or organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类别的任何成员或诸如细菌分类家族，属或物种的任何成员的生物体和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

3. 发明授权

US5932416A Method for detecting the presence of RNA belonging to an organ or tissue cell-type 失效
标题翻译：用于检测属于器官或组织细胞类型的RNA的存在的方法
公开(公告)号：US5932416A
公开(公告)日：1999-08-03
申请号：US459650
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶洗涤剂混合物接触，以使样品中的生物体或病毒的核酸更易于进行杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

4. 发明授权

US5723597A Ribosomal nucleic acid probes for detecting organisms or groups of organisms 失效
标题翻译：核糖体核酸探针，用于检测有机体或生物群体
公开(公告)号：US5723597A
公开(公告)日：1998-03-03
申请号：US199486
申请日：1994-02-22
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

5. 发明授权

US5714324A Methods for producing hybridization probes specific for rRNA subunit subsequences 失效
标题翻译：用于产生rRNA亚基亚型特异性杂交探针的方法
公开(公告)号：US5714324A
公开(公告)日：1998-02-03
申请号：US459837
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/00
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree or hybridization that has occurred. The method may include contacting, a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence or substances with antimicrobial activity, and to determine the state of growth or microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的程度或杂交。该方法可以包括使样品与酶 - 洗涤剂混合物接触，以使样品中的生物体或病毒的核酸更易于进行杂交。该方法还可以用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的存在或物质，以及确定生长状态或微生物和其它细胞。

6. 发明授权

US5288611A Method for detecting, identifying, and quantitating organisms and viruses 失效
标题翻译：检测，鉴定和定量生物体和病毒的方法
公开(公告)号：US5288611A
公开(公告)日：1994-02-22
申请号：US857081
申请日：1992-03-19
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C12Q1/06 , C12Q1/22
CPC分类号： C12Q1/689 , C12Q1/18 , C12Q1/6888
摘要： A method and probes for specifically and sensitively detecting, identifying, and quantitating any organism category or group of organisms containing RNA in a sample is disclosed. The nucleic acids of the organisms present in the sample are brought together with a marked probe comprising nucleic acid molecules which are complementary only to nucleic acid containing sequences complementary to RNA sequences known to be conserved in an organism, category or group of organisms. The probe in sample nucleic acid mixture is incubated under nucleic acid hybridization conditions and then assayed to determine the degree of hybridization that has occurred. Hybridization indicates the presence and identity of the organism, category or group of organisms in the sample. The quantity of RNA present in the sample can be determined and compared to that normally present in the known organisms to determine the number of organisms present. Batteries of sequentially more specific probes can also be utilized.
摘要翻译：公开了一种用于特异性和敏感地检测，鉴定和定量样品中含有RNA的生物类或生物体组的方法和探针。将存在于样品中的生物体的核酸与包含核酸分子的标记探针一起使用，所述核酸分子与仅在生物，类别或生物体群体中保守的RNA序列互补的含有核酸的序列互补。将样品核酸混合物中的探针在核酸杂交条件下培养，然后测定以确定发生的杂交程度。杂交表明样品中有机体，类别或生物体的存在和身份。可以确定样品中存在的RNA的量并将其与已知生物体中通常存在的RNA进行比较，以确定存在的生物体的数量。还可以使用顺序地更具体的探针的电池。

7. 发明授权

US5955261A Method for detecting the presence of group-specific viral mRNA in a sample 失效
标题翻译：用于检测样品中组特异性病毒mRNA的存在的方法
公开(公告)号：US5955261A
公开(公告)日：1999-09-21
申请号：US459276
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/70 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/701
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid and molecules complementary to RNA or other nucleic sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与所述生物体的RNA或其他核酸序列互补的核酸和分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

8. 发明授权

US5928864A Method for determining the presence of organisms in a sample by detecting transfer nucleic acid 失效
标题翻译：通过检测转移核酸来确定样品中有机体存在的方法
公开(公告)号：US5928864A
公开(公告)日：1999-07-27
申请号：US456664
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

9. 发明授权

US5738988A Method for detecting antimicrobial agents or unknown organisms in a sample using ribosomal probe hybridization 失效
标题翻译：使用核糖体探针杂交检测样品中的抗微生物剂或未知有机体的方法
公开(公告)号：US5738988A
公开(公告)日：1998-04-14
申请号：US459843
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family. Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth microorganism and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，任何大型，中等或小类生物（如细菌分类家族成员）的成员的生物体的方法。属，或物种，以前未知的生物。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可以用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定生长微生物和其它细胞的状态。

10. 发明授权

US5641632A Method for preparing rRNA for hybridization with a probe 失效
标题翻译：用于与探针杂交的rRNA的制备方法
公开(公告)号：US5641632A
公开(公告)日：1997-06-24
申请号：US460532
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6888 , C12Q1/18 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

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