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1. 发明授权

US06586193B2 Analyte assay using particulate labels 失效
公开(公告)号：US06586193B2
公开(公告)日：2003-07-01
申请号：US08953713
申请日：1997-10-17
申请人： Juan Yguerabide , Evangelina E. Yguerabide , David E. Kohne , Jeffrey T. Jackson
发明人： Juan Yguerabide , Evangelina E. Yguerabide , David E. Kohne , Jeffrey T. Jackson
IPC分类号： C12Q168
CPC分类号： G01N33/54313 , C12Q1/6816 , C12Q1/6834 , G01N15/14 , G01N15/1425 , G01N15/1459 , G01N33/585 , G01N2015/0038 , G01N2015/1493 , Y10S436/805 , Y10S436/817 , C12Q2563/103
摘要： Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

2. 发明授权

US5612183A Method for determining the effect of antimicrobial agents on growth using ribosomal nucleic acid subunit subsequence specific probes 失效
标题翻译：使用核糖体核酸亚基亚序列特异性探针测定抗微生物剂对生长的影响的方法
公开(公告)号：US5612183A
公开(公告)日：1997-03-18
申请号：US460531
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/18 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category or organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类别的任何成员或诸如细菌分类家族，属或物种的任何成员的生物体和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

3. 发明授权

US5955261A Method for detecting the presence of group-specific viral mRNA in a sample 失效
标题翻译：用于检测样品中组特异性病毒mRNA的存在的方法
公开(公告)号：US5955261A
公开(公告)日：1999-09-21
申请号：US459276
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/70 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/701
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid and molecules complementary to RNA or other nucleic sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与所述生物体的RNA或其他核酸序列互补的核酸和分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

4. 发明授权

US5928864A Method for determining the presence of organisms in a sample by detecting transfer nucleic acid 失效
标题翻译：通过检测转移核酸来确定样品中有机体存在的方法
公开(公告)号：US5928864A
公开(公告)日：1999-07-27
申请号：US456664
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

5. 发明授权

US5738988A Method for detecting antimicrobial agents or unknown organisms in a sample using ribosomal probe hybridization 失效
标题翻译：使用核糖体探针杂交检测样品中的抗微生物剂或未知有机体的方法
公开(公告)号：US5738988A
公开(公告)日：1998-04-14
申请号：US459843
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family. Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth microorganism and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，任何大型，中等或小类生物（如细菌分类家族成员）的成员的生物体的方法。属，或物种，以前未知的生物。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可以用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定生长微生物和其它细胞的状态。

6. 发明授权

US5641632A Method for preparing rRNA for hybridization with a probe 失效
标题翻译：用于与探针杂交的rRNA的制备方法
公开(公告)号：US5641632A
公开(公告)日：1997-06-24
申请号：US460532
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6888 , C12Q1/18 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

7. 发明授权

US5567587A Method for detecting, the presence and amount of prokaryotic organisms using specific rRNA subsequences as probes 失效
标题翻译：用特异性rRNA亚序列作为探针检测原核生物的存在和数量的方法
公开(公告)号：US5567587A
公开(公告)日：1996-10-22
申请号：US460735
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/18 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species,and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and, other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可以用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，以及确定微生物和其它细胞的生长状态。

8. 发明授权

US5132207A Accelerated nucleic acid reassociation method 失效
标题翻译：加速核酸重组方法
公开(公告)号：US5132207A
公开(公告)日：1992-07-21
申请号：US644879
申请日：1991-01-23
申请人： David E. Kohne , Daniel L. Kacian
发明人： David E. Kohne , Daniel L. Kacian
IPC分类号： C07H21/00 , C12Q1/68
CPC分类号： C07H21/00 , C12Q1/6806 , C12Q1/6832 , C12Q1/689
摘要： A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of reaction is greatly increased over the rate of reaction at standard reference conditions. The greatly accelerated reaction rate is accomplished through the use of known concentrations of nucleic acid precipitating agents which are added to the reaction solution. Nucleic acid denaturing agents may also be added. The solution so formed is incubated and then assayed for the presence of double stranded nucleic acid molecules.
摘要翻译：公开了在单相反应溶液中从单独的单链核酸分子形成双链核酸分子的方法，其中在标准参考条件下反应速率大大地高于反应速率。通过使用已知浓度的加入到反应溶液中的核酸沉淀剂来实现极大加速的反应速率。还可加入核酸变性剂。孵育形成的溶液，然后测定双链核酸分子的存在。

9. 发明授权

US5932416A Method for detecting the presence of RNA belonging to an organ or tissue cell-type 失效
标题翻译：用于检测属于器官或组织细胞类型的RNA的存在的方法
公开(公告)号：US5932416A
公开(公告)日：1999-08-03
申请号：US459650
申请日：1995-06-02
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶洗涤剂混合物接触，以使样品中的生物体或病毒的核酸更易于进行杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

10. 发明授权

US5723597A Ribosomal nucleic acid probes for detecting organisms or groups of organisms 失效
标题翻译：核糖体核酸探针，用于检测有机体或生物群体
公开(公告)号：US5723597A
公开(公告)日：1998-03-03
申请号：US199486
申请日：1994-02-22
申请人： David E. Kohne
发明人： David E. Kohne
IPC分类号： C12Q1/18 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/18 , C12Q1/6888 , C12Q1/689
摘要： A method for detecting and quantitating organisms containing R-RNA, t-RNA, other RNA, any member of a large, intermediate or small category of organisms such as any member of a bacterial taxonomic Family, Genus, or Species, and previously unknown organisms. The method comprises contacting the nucleic acid of the organisms whose presence, identification and quantitation are to be determined, with a marked probe comprising nucleic acid molecules complementary to RNA or other nucleic acid sequences, of the said organism, under nucleic acid hybridization conditions, and then determining the degree of hybridization that has occurred. The method may include contacting a sample with an enzyme-detergent mixture to make the nucleic acids of the organism or virus in a sample more readily available for hybridization. The method can also be used to determine the sensitivity of particular groups of organisms to antimicrobial agents, to determine the presence of substances with antimicrobial activity, and to determine the state of growth of microorganisms and other cells.
摘要翻译：一种用于检测和定量含有R-RNA，t-RNA，其他RNA，大型，中等或小类生物体的任何成员（如细菌分类家族，属或物种的任何成员）和以前未知生物体的生物体的方法。该方法包括在核酸杂交条件下将存在，鉴定和定量待测的生物的核酸与包含与RNA或其他核酸序列互补的核酸分子的标记探针接触，以及然后确定发生的杂交程度。该方法可以包括使样品与酶 - 洗涤剂混合物接触以使样品中的生物或病毒的核酸更容易用于杂交。该方法还可用于确定特定群体的微生物对抗微生物剂的敏感性，以确定具有抗微生物活性的物质的存在，并确定微生物和其它细胞的生长状态。

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