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    • 1. 发明申请
    • Microfluidic interface for highly parallel addressing of sensing arrays
    • 用于感测阵列高度并行寻址的微流体接口
    • US20070231880A1
    • 2007-10-04
    • US11241060
    • 2005-09-30
    • David Chang-YenSriram NatarajanJosh EckmanBruce GaleDavid Myszka
    • David Chang-YenSriram NatarajanJosh EckmanBruce GaleDavid Myszka
    • C12M3/00G01D15/02
    • G01N33/553B01J19/0046B01L3/0293B01L3/5025B01L3/502715B01L3/50273B01L2200/021B01L2300/0636B01L2300/0819B01L2300/0822B01L2300/0877B01L2400/0487C12N15/1093G01N35/1074G01N2035/00158G01N2035/1039Y10T436/2575
    • Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells, microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on a separate surface. Each orifice is connected to two wells via microconduits. When the spotter contacts a surface, a seal is formed between the orifices and the surface. The same or different substances may be flowed across each orifice. Any number of orifices may be incorporated into a spotter. The spotter is particularly useful for depositing proteins in high concentrations on a surface, since the spotter may be placed on a surface for an extended period of time.
    • 公开了一种用于形成微量测定器,生物芯片,生物传感器和细胞培养物的检测器装置和方法。 斑点斑块可用于将高度浓缩的蛋白质或其他材料的斑点沉积在微阵列载玻片,晶片或其它表面上。 它也可以用于在同一点上执行各种化学步骤。 点样器通过将所需物质(或其溶液)流过斑点区域直到达到表面饱和为止,增加每个斑点处物质的表面密度。 识别器可以由井板处理设备装载。 点样器使用井,微流体管道和孔口将蛋白质,其他生物分子或化学品沉积在单独表面上的一个点上。 每个孔通过微管连接到两个孔。 当检测器接触表面时,在孔和表面之间形成密封。 相同或不同的物质可能流过每个孔口。 任何数量的孔口都可以并入到一个定点器中。 鉴定器对于在表面上沉积高浓度的蛋白质是特别有用的,因为可以将定位器放置在表面上较长时间。