专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5635346A Assay for Non-A Non-B hepatitis 失效
标题翻译：非甲非乙型肝炎检测
公开(公告)号：US5635346A
公开(公告)日：1997-06-03
申请号：US372723
申请日：1995-01-17
申请人： David C. Leahy , John A. Todd , Michael E. Jolley , Dinesh O. Shah , Delia R. Bethell , Terukatsu Arima
发明人： David C. Leahy , John A. Todd , Michael E. Jolley , Dinesh O. Shah , Delia R. Bethell , Terukatsu Arima
IPC分类号： A61K39/00 , C07K14/005 , C07K14/18 , G01N33/53 , G01N33/543 , G01N33/551 , G01N33/553 , G01N33/576 , C12Q1/70
CPC分类号： C07K14/005 , G01N33/5767 , C12N2770/24222
摘要： New assays for diagnosing NANBH utilizing novel peptide fragments derived from polypeptide antigens reactive to antibodies present in the sera of infected patients are disclosed. In producing these peptides, the portion of the polypeptide contributing to high backgrounds is deleted thereby resulting in assays with an exceptionally high signal to background ratio.
摘要翻译：公开了利用衍生自对感染患者血清中存在的抗体具有反应性的多肽抗原的新肽片段来诊断NANBH的新的测定法。在生产这些肽时，有助于高背景的多肽的部分被缺失，从而导致具有非常高的信号与背景比的测定。

2. 发明授权

US07811775B2 Fluorescence polarization-based homogenous assay for aflatoxins 有权
标题翻译：基于荧光偏振的黄曲霉毒素同源测定
公开(公告)号：US07811775B2
公开(公告)日：2010-10-12
申请号：US12127350
申请日：2008-05-27
申请人： Mohammad Sarwar Nasir , Michael E. Jolley
发明人： Mohammad Sarwar Nasir , Michael E. Jolley
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , G01N33/582
摘要： A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
摘要翻译：用于确定农产品中黄曲霉毒素含量的均匀测定法使用荧光偏振技术。使用溶剂从农产品样品中提取黄曲霉毒素。通过将提取物与示踪剂和特异于黄曲霉毒素的单克隆抗体组合来制备混合物。示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。通过将黄曲霉毒素与合适的荧光团缀合来制备示踪剂。测量混合物的荧光偏振。可以使用通过测量已知浓度的一系列黄曲霉毒素溶液的荧光偏振获得的标准曲线来计算混合物的黄曲霉毒素浓度。

3. 发明授权

US06596546B1 Detection of antibodies to bacterial antigens by fluorescence polarization 有权
标题翻译：通过荧光偏振检测细菌抗原的抗体
公开(公告)号：US06596546B1
公开(公告)日：2003-07-22
申请号：US09400798
申请日：1999-09-22
申请人： Michael E. Jolley , Klaus H. Nielsen
发明人： Michael E. Jolley , Klaus H. Nielsen
IPC分类号： G01N33533
CPC分类号： G01N33/56916 , G01N33/56911 , G01N33/5695 , G01N2333/23 , G01N2333/35 , G01N2400/50 , Y02A50/56
摘要： In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
摘要翻译：在均相免疫测定中，使荧光团结合的脂多糖衍生的细菌抗原与抗原特异性抗体反应。通过测量复合物形成后荧光极化的变化，可以获得免疫复合物形成的定量检测。反应快速发生（不到两分钟至平衡），并且仅向稀释的血清样品中加入一种试剂。没有固相分离步骤消除了假阳性结果并增加了产量。

4. 发明授权

US4420568A Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins 失效
标题翻译：荧光偏振免疫测定利用取代的三嗪基氨基荧光素
公开(公告)号：US4420568A
公开(公告)日：1983-12-13
申请号：US325872
申请日：1981-11-30
申请人： Chao-Huei J. Wang , Stephen D. Stroupe , Michael E. Jolley
发明人： Chao-Huei J. Wang , Stephen D. Stroupe , Michael E. Jolley
IPC分类号： C07D473/08 , C07H15/236 , G01N33/533 , G01N33/54 , G01N33/52 , G01N33/58 , G01N33/74
CPC分类号： C07H15/234 , C07D473/08 , G01N33/533 , Y10S436/80 , Y10S436/817
摘要： An immunochemical method and reagent compounds for determining ligands in a sample. The reagent compounds are derivatives of a triazinylaminofluorescein and are represented by the structural formula ##STR1## wherein Y is halo or lower alkyl; andR is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody.The reagent compound and an antibody specific to the ligand are added to the sample in a fluorescence polarization immunoassay.
摘要翻译：用于测定样品中配体的免疫化学方法和试剂化合物。试剂化合物是三嗪基氨基荧光素的衍生物，其结构式为：其中Y为卤素或低级烷基; 并且R是配体 - 类似物，其中所述配体 - 类似物与所述配体具有至少一个共同表位，以便被共同抗体特异性地识别。将试剂化合物和对配体特异的抗体以荧光偏振免疫测定法加入到样品中。

5. 发明授权

US4131798A Array gamma counter 失效
标题翻译：阵列伽玛计数器
公开(公告)号：US4131798A
公开(公告)日：1978-12-26
申请号：US784900
申请日：1977-04-05
申请人： Reddy R. V. Reddy , Michael E. Jolley
发明人： Reddy R. V. Reddy , Michael E. Jolley
IPC分类号： G01T1/24 , G01T7/08 , G01T1/00
CPC分类号： G01T1/247 , G01T1/24 , G01T7/08
摘要： A radioactive particle counter for a plurality of discrete radioactive particle emitting samples, including a plurality of detectors for each sample to simultaneously detect the respective emitted radioactive particles and provide a respective electrical signal, a plurality of storage counters for simultaneously accumulating the respective sample count during a predetermined counting interval, and a read-out control unit for periodically, sequentially reading out the accumulated sample count in each storage counter and coupling same to a control counter for temporary display of the accumulated sample count or for eventually totalling of the respective sample count.
摘要翻译：一种用于多个离散放射性粒子发射样品的放射性粒子计数器，包括用于每个样本的多个检测器，用于同时检测各个发射的放射性粒子并提供相应的电信号;多个存储计数器，用于在预定的计数间隔和读出控制单元，用于周期性地顺序地读出每个存储计数器中累积的采样计数并将其耦合到控制计数器，以暂时显示累积的采样计数或最终合计各个采样计数。

6. 发明申请

US20080227221A1 Fluorescence Polarization-Based Homogeneous Assay For Aflatoxins 有权
标题翻译：基于荧光偏振的黄曲霉毒素均相测定
公开(公告)号：US20080227221A1
公开(公告)日：2008-09-18
申请号：US12127350
申请日：2008-05-27
申请人： Mohammad Sarwar Nasir , Michael E. Jolley
发明人： Mohammad Sarwar Nasir , Michael E. Jolley
IPC分类号： G01N33/536
CPC分类号： G01N33/542 , G01N33/582
摘要： A homogeneous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.
摘要翻译：用于确定农产品中黄曲霉毒素含量的均匀测定法使用荧光偏振技术。使用溶剂从农产品样品中提取黄曲霉毒素。通过将提取物与示踪剂和特异于黄曲霉毒素的单克隆抗体组合来制备混合物。示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。通过将黄曲霉毒素与合适的荧光团缀合来制备示踪剂。测量混合物的荧光偏振。可以使用通过测量已知浓度的一系列黄曲霉毒素溶液的荧光偏振获得的标准曲线来计算混合物的黄曲霉毒素浓度。

7. 发明授权

US5976820A Detection of antibodies to bacterial antigens by flourescence polarization 失效
标题翻译：通过荧光极化检测细菌抗原的抗体
公开(公告)号：US5976820A
公开(公告)日：1999-11-02
申请号：US520313
申请日：1995-08-28
申请人： Michael E. Jolley , Klaus H. Nielsen
发明人： Michael E. Jolley , Klaus H. Nielsen
IPC分类号： G01N33/569 , G01N33/554 , G01N33/53 , C07K1/00
CPC分类号： G01N33/56916 , G01N33/56911 , G01N33/5695 , G01N2333/23 , G01N2333/35 , G01N2400/50
摘要： In a homogeneous immunoassay, fluorophore-conjugated lipopolysaccharide derived bacterial antigens are reacted with antibodies specific for the antigens. Quantitative detection of the formation of an immune complex is obtained by measuring the change in fluorescence polarization after complex formation. The reaction occurs quickly (less than two minutes to equilibrium), and involves the addition of only one reagent to a diluted serum specimen. The absence of a solid phase separation step eliminates false positive results and increases throughput.
摘要翻译：在均相免疫测定中，使荧光团结合的脂多糖衍生的细菌抗原与抗原特异性抗体反应。通过测量复合物形成后荧光极化的变化，可以获得免疫复合物形成的定量检测。反应快速发生（不到两分钟至平衡），并且仅向稀释的血清样品中加入一种试剂。没有固相分离步骤消除了假阳性结果并增加了产量。

8. 发明授权

US5391740A Fluoresence polarization immunoassay 失效
标题翻译：荧光极化免疫测定
公开(公告)号：US5391740A
公开(公告)日：1995-02-21
申请号：US044927
申请日：1993-04-08
申请人： Chao-Huei J. Wang , Stephen D. Stroupe , Michael E. Jolley
发明人： Chao-Huei J. Wang , Stephen D. Stroupe , Michael E. Jolley
IPC分类号： C07D311/90 , C07D405/12 , G01N33/533 , C07D223/24 , C07D239/54
CPC分类号： C07D405/12 , C07D311/90 , G01N33/533
摘要： This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of the specific ligand present in a sample.
摘要翻译：本公开涉及用于测定生物流体如血清，血浆，脊髓液，羊膜液和尿液中配体的方法和试剂。特别地，本发明涉及荧光偏振免疫测定方法以及在这种方法中用作试剂的新型示踪剂化合物。所公开的方法将免疫测定的特异性与荧光偏振技术的速度和方便性相结合，以提供测定样品中存在的特异性配体的量的方法。

9. 发明授权

US4652533A Method of solid phase immunoassay incorporating a luminescent label 失效
标题翻译：包含发光标签的固相免疫测定方法
公开(公告)号：US4652533A
公开(公告)日：1987-03-24
申请号：US489519
申请日：1983-04-28
申请人： Michael E. Jolley
发明人： Michael E. Jolley
IPC分类号： G01N33/543 , G01N33/544 , G01N33/573 , G01N33/58 , G01N33/546 , G01N33/554 , G01N33/569
CPC分类号： G01N33/54313 , G01N33/544 , G01N33/573 , G01N33/585 , G01N33/586 , Y10S435/968 , Y10S436/807 , Y10S436/809
摘要： A method is provided for solid phase immunoassay for quantitation of antigen, hapten or antibody analyte in liquid sample and alternatively for quantitation of analyte occuring on or attached to cells or other particulate material in a liquid sample. The solid phase is suspended for at least the initial reactions and is subsequently concentrated by microfiltration to a volume substantially less than the sample volume. Luminescence of substantially the entire label on the concentrated solid phase is measured.
摘要翻译：提供了一种用于定量液体样品中抗原，半抗原或抗体分析物的固相免疫测定方法，或者用于定量在液体样品中细胞或其他颗粒物质上发生或附着于其上的分析物。将固相悬浮至少初始反应，随后通过微量过滤浓缩至基本上小于样品体积的体积。测量浓缩固相上基本上整个标签的发光。

10. 发明授权

US06350574B1 Fluorescence polarization—based diagnostic assay for equine infectious anemia virus 有权
标题翻译：基于荧光偏振的马传染性贫血病毒诊断试验
公开(公告)号：US06350574B1
公开(公告)日：2002-02-26
申请号：US09400564
申请日：1999-09-21
申请人： Ronald C. Montelaro , Sarah B. Tencza , Michael E. Jolley , Mohammad S. Nasir
发明人： Ronald C. Montelaro , Sarah B. Tencza , Michael E. Jolley , Mohammad S. Nasir
IPC分类号： C12Q170
CPC分类号： G01N33/533 , G01N33/56983 , G01N2333/155 , Y10S435/968
摘要： A fluorescence polarization assay for Equine Infectious Anemia Virus utilizes a short peptide reagent probe derived from a conserved immunodominant region of gp45. The probe is N-terminally labeled, preferably with 6-carboxyfluorescein, and purified by HPLC, which reacts in a homogenous assay with anti-EIAV antibodies contained in the serum of field infected horses and ponies. The assay has a sensitivity of about 90 percent with a specificity approaching 100 percent.
摘要翻译：马传染性贫血病毒的荧光偏振测定利用源自gp45的保守免疫显性区域的短肽试剂探针。探针是N-末端标记的，优选用6-羧基荧光素，并通过HPLC纯化，其在均匀测定中与包含在场感染的马和小马血清中的抗EIAV抗体反应。该测定具有约90％的灵敏度，特异性接近100％。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式