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1. 发明授权

US07354750B2 Methods for separating molecules 失效
标题翻译：分离分子的方法
公开(公告)号：US07354750B2
公开(公告)日：2008-04-08
申请号：US10689368
申请日：2003-10-20
申请人： Daniel J. Simpson , Tonny Johnson , John Shultz , Roderick G. Flemming , Rebecca Godat , Sanchayita Kar , Robin Hurst
发明人： Daniel J. Simpson , Tonny Johnson , John Shultz , Roderick G. Flemming , Rebecca Godat , Sanchayita Kar , Robin Hurst
IPC分类号： C12N11/00 , B01J20/22 , B01J20/10 , B01J20/283 , B01J29/04
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.
摘要翻译：本发明提供使用金属改性固体支持物分离金属或分子例如多肽，核酸或内毒素的组合物和方法。使用本发明的改性固体载体，从原料中分离金属或分子。本发明还提供了可以结合本发明方法使用的试剂盒。

2. 发明授权

US07112552B2 Compositions for separating molecules 失效
标题翻译：用于分离分子的组合物
公开(公告)号：US07112552B2
公开(公告)日：2006-09-26
申请号：US10689176
申请日：2003-10-20
申请人： Daniel J. Simpson , Tonny Johnson , Roderick G. Flemming
发明人： Daniel J. Simpson , Tonny Johnson , Roderick G. Flemming
IPC分类号： B01J20/22 , B01J20/10 , B01J20/283 , B01J29/04 , C12N11/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.
摘要翻译：本发明提供了使用改性固体支持物分离金属或生物分子例如多肽，核酸或内毒素的组合物。

3. 发明授权

US07304015B2 Compositions for separating molecules 失效
标题翻译：用于分离分子的组合物
公开(公告)号：US07304015B2
公开(公告)日：2007-12-04
申请号：US11201714
申请日：2005-08-11
申请人： Daniel J. Simpson , Tonny Johnson , Roderick G. Flemming
发明人： Daniel J. Simpson , Tonny Johnson , Roderick G. Flemming
IPC分类号： B01J20/22 , B01J20/10 , B01J20/283 , B01J29/04 , C12N11/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.
摘要翻译：本发明提供了使用改性固体支持物分离金属或生物分子例如多肽，核酸或内毒素的组合物。

4. 发明授权

US06787307B1 Lysate clearance and nucleic acid isolation using silanized silica matrices 有权
标题翻译：裂解液清除和使用硅烷化二氧化硅基质的核酸分离
公开(公告)号：US06787307B1
公开(公告)日：2004-09-07
申请号：US09711782
申请日：2000-11-13
申请人： Rex M. Bitner , Daniel J. Simpson , Roderick G. Flemming , Susan C. Koller
发明人： Rex M. Bitner , Daniel J. Simpson , Roderick G. Flemming , Susan C. Koller
IPC分类号： C12Q168
CPC分类号： C12N15/1006 , C12N1/02 , C12N15/1013 , C12Q1/6806 , Y10T436/143333
摘要： A method is provided for using a silanized silica matrix to isolate a target nucleic acids, such as plasmid DNA, fragments of DNA, chromosomal DNA, or RNA from contaminants, including proteins, lipids, cellular debris, or non-target nucleic acids. The silanized silica matrix comprises a silica based solid phase and a plurality of silane ligands covalently attached to the surface of the solid phase. Non-target material adsorbs to the silanized silica matrix in the presence of a sufficient concentration of chaotropic salt, while target nucleic acids adsorb to the matrix under other solution conditions. The method of using the silanized silica matrix of the present invention can be used to clear solutions of disrupted biological material, and to isolate nucleic acids therefrom or from other solutions containing nucleic acids and at least one contaminant.
摘要翻译：提供了一种使用硅烷化二氧化硅基质从污染物（包括蛋白质，脂质，细胞碎片或非目标核酸）分离靶核酸（如质粒DNA），DNA片段，染色体DNA或RNA的方法。硅烷化二氧化硅基质包含二氧化硅基固相和共价连接到固相表面的多个硅烷配体。非目标物质在足够浓度的离液盐存在下吸附到硅烷化二氧化硅基质上，而靶核酸在其它溶液条件下吸附到基质上。使用本发明的硅烷化二氧化硅基质的方法可用于清除破坏的生物材料的溶液，并从其中分离核酸或从其它含有核酸和至少一种污染物的溶液中分离出核酸。

5. 发明授权

US07304016B2 Compositions for separating molecules 失效
公开(公告)号：US07304016B2
公开(公告)日：2007-12-04
申请号：US11419230
申请日：2006-05-19
申请人： Daniel J. Simpson , Tonny M. Johnson , Roderick G. Flemming
发明人： Daniel J. Simpson , Tonny M. Johnson , Roderick G. Flemming
IPC分类号： B01J20/22 , B01J20/10 , B01J20/283 , B01J29/04 , C12N11/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.

6. 发明申请

US20080193996A1 METHODS FOR SEPARATING MOLECULES 审中-公开
标题翻译：分离分子的方法
公开(公告)号：US20080193996A1
公开(公告)日：2008-08-14
申请号：US12019985
申请日：2008-01-25
申请人： Daniel J. Simpson , Tonny Johnson , John Schultz , Rebecca Godat , Sanchayita Kar
发明人： Daniel J. Simpson , Tonny Johnson , John Schultz , Rebecca Godat , Sanchayita Kar
IPC分类号： C12N11/00 , C07K14/00 , C07K14/805
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.
摘要翻译：本发明提供使用金属改性固体支持物分离金属或分子例如多肽，核酸或内毒素的组合物和方法。使用本发明的改性固体载体，从原料中分离金属或分子。本发明还提供了可以结合本发明方法使用的试剂盒。

7. 发明申请

US20130071818A1 METHODS FOR SELLING WINE AND OTHER FOODS AND DRINKS 审中-公开
标题翻译：用于出售葡萄酒和其他食物和饮料的方法
公开(公告)号：US20130071818A1
公开(公告)日：2013-03-21
申请号：US13423258
申请日：2012-03-18
申请人： Robert E. Buxton, JR. , Daniel J. Simpson
发明人： Robert E. Buxton, JR. , Daniel J. Simpson
IPC分类号： G09B19/00
CPC分类号： G09B19/0092 , A23C19/00 , A23L2/00 , C12C11/11 , C12G1/00
摘要： This invention relates to the labeling of wine for descriptive, educational, and informational purposes. Specifically, in preferred embodiments, labels are provided that display graphically the multifactorial components of taste intensity, taste location on the palate, and tasting descriptors, optionally with supplementary information (type of grape, region where wine is produced, smell of the wine, etc). The methods described herein are further extended to a wide variety of foods and drinks
摘要翻译：本发明涉及用于描述性，教育性和信息性目的的葡萄酒标签。具体地，在优选实施例中，提供标签，其以图形方式显示味道强度，口感上的味觉位置和品尝描述符的多因素分量，以及品尝描述符，可选地包含补充信息（葡萄种类，葡萄酒生产区域，葡萄酒气味等））。本文描述的方法进一步扩展到各种各样的食物和饮料

8. 发明申请

US20090220651A1 METHODS FOR CHARACTERIZING FOODS AND DRINKS 审中-公开
标题翻译：表征食物和饮料的方法
公开(公告)号：US20090220651A1
公开(公告)日：2009-09-03
申请号：US12390401
申请日：2009-02-20
申请人： Robert E. Buxton , Daniel J. Simpson
发明人： Robert E. Buxton , Daniel J. Simpson
IPC分类号： A23P1/00 , G09F3/00
CPC分类号： G09B19/0092 , A23C19/00 , A23L2/00 , C12C11/11 , C12G1/00
摘要： This invention relates to the labeling of wine for descriptive, educational, and informational purposes. Specifically, in preferred embodiments, labels are provided that display graphically the multifactorial components of taste intensity, taste location on the palate, and tasting descriptors, optionally with supplementary information (type of grape, region where wine is produced, smell of the wine, etc). The methods described herein are further extended to a wide variety of foods and drinks.
摘要翻译：本发明涉及用于描述性，教育性和信息性目的的葡萄酒标签。具体地，在优选实施例中，提供标签，其以图形方式显示味道强度，口感上的味觉位置和品尝描述符的多因素分量，以及品尝描述符，可选地包含补充信息（葡萄种类，葡萄酒生产区域，葡萄酒气味等））。本文描述的方法进一步扩展到各种各样的食物和饮料。

9. 发明授权

US06270970B1 Mixed-bed solid phase and its use in the isolation of nucleic acids 失效
标题翻译：混合床固相及其在核酸分离中的应用
公开(公告)号：US06270970B1
公开(公告)日：2001-08-07
申请号：US09312139
申请日：1999-05-14
申请人： Craig E. Smith , Diana L. Holmes , Daniel J. Simpson , Jehoshua Katzenhendler , Rex M. Bitner , Josephine C. Grosch
发明人： Craig E. Smith , Diana L. Holmes , Daniel J. Simpson , Jehoshua Katzenhendler , Rex M. Bitner , Josephine C. Grosch
IPC分类号： C12Q168
CPC分类号： C12N15/1006 , B01J41/20 , B01J47/04 , C12N15/101 , C12N15/1013 , Y10S435/803 , Y10T436/107497
摘要： Mixed-bed solid phases are provided, with methods for using such solid phases to isolate target nucleic acids, such as plasmid DNA, chromosomal DNA, RNA, or nucleic acids generated by enzymatic amplification from contaminants, including proteins, lipids, cellular debris, or other nucleic acids. The mixed-bed solid phases of this invention are mixtures of at least two different solid phases, each of which has a capacity to bind to the target nucleic acid under different solution conditions, and the capacity to release the nucleic acid under similar elution conditions. By exchanging solution conditions according to the methods of this invention, one can remove contaminants from the target nucleic acid bound to the mixed-bed solid phase, then elute the target nucleic acid in an elution buffer.
摘要翻译：提供了混合床固相，使用这种固相来分离靶核酸，如质粒DNA，染色体DNA，RNA或通过酶学扩增产生的核酸，包括蛋白质，脂质，细胞碎片或其他核酸。本发明的混合床固相是至少两种不同固相的混合物，每种固相具有在不同溶液条件下与靶核酸结合的能力，以及在类似洗脱条件下释放核酸的能力。通过根据本发明的方法交换溶液条件，可以从与混合床固相结合的靶核酸中除去污染物，然后在洗脱缓冲液中洗脱目标核酸。

10. 发明授权

US08535542B2 Filter press with integrated radio frequency heating 失效
标题翻译：压滤机采用集成射频加热
公开(公告)号：US08535542B2
公开(公告)日：2013-09-17
申请号：US12590150
申请日：2009-11-02
申请人： Daniel J. Simpson , Ferdinand Kogler
发明人： Daniel J. Simpson , Ferdinand Kogler
IPC分类号： B01D25/21 , B01D25/12
CPC分类号： B01D25/284 , B01D25/164 , B01D25/215 , B01D25/285 , B01D25/322
摘要： A method of separating a mixture of liquid and insoluble solids in a filter press may comprise: pumping the mixture into a chamber between two filter plates in the filter press to form a filter cake, wherein the chamber is lined by filter cloths, and wherein, during the pumping, filtrate is forced through the filter cloths and out of the chamber; heating the filter cake in the chamber, wherein, during the heating, filtrate is forced through the filter cloths and out of the chamber, and wherein the heating is by radio frequency irradiation of the filter cake in the chamber; and releasing dried filter cake from the chamber. A filter press system for separating a mixture of liquid and insoluble solids may comprise: a frame; a plurality of filter plates configured to form a stack of parallel plates, each of the plurality of filter plates being movably attached to the frame, the plurality of filter plates further being configured to form a multiplicity of chambers, each of the multiplicity of chambers being lined by filter cloths, wherein the plurality of filter plates, the multiplicity of chambers and the filter cloths are configured to allow filtrate to escape from the chambers while retaining solids from the mixture to form a filter cake; and a radio frequency heater, for directly heating the filter cake in the multiplicity of chambers. The radio frequency heating may include microwave heating, or dielectric heating by lower frequency radio waves in the range of 1 to 100 MHz.
摘要翻译：在压滤机中分离液体和不溶性固体的混合物的方法可以包括：将混合物泵入压滤机中的两个过滤板之间的室中以形成滤饼，其中室由滤布排列，其中，在泵送期间，滤液被迫通过滤布并离开室; 加热室中的滤饼，其中，在加热期间，滤液被迫通过滤布并离开室，并且其中加热是通过在该室中的滤饼的射频照射; 并从室中释放干燥的滤饼。用于分离液体和不溶性固体的混合物的压滤机系统可以包括：框架; 多个过滤板被构造成形成一组平行板，所述多个过滤板中的每一个可移动地附接到所述框架，所述多个过滤板还构造成形成多个室，所述多个室中的每一个均为由滤布排列，其中多个过滤板，多个室和滤布构造成允许滤液从室中逸出，同时保持来自混合物的固体以形成滤饼; 和用于直接加热多个室中的滤饼的射频加热器。射频加热可以包括微波加热，或者通过1到100MHz范围内的低频无线电波的介电加热。

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