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1. 发明申请

US20060153870A1 Method for the recovery of non-segmented, nagative-stranded RNA viruses from cDNA 审中-公开
标题翻译：从cDNA中回收非分段的，潜伏性RNA病毒的方法
公开(公告)号：US20060153870A1
公开(公告)日：2006-07-13
申请号：US11290639
申请日：2005-11-29
申请人： Christopher Parks , Stephen Udem , Modinderjit Sidhu
发明人： Christopher Parks , Stephen Udem , Modinderjit Sidhu
IPC分类号： A61K39/12 , C12N7/00 , C12N15/86
CPC分类号： C07K14/005 , A61K39/00 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N15/86 , C12N2760/18022 , C12N2760/18422 , C12N2760/18451 , C12N2760/18452 , C12N2760/18551 , C12N2760/18552 , C12N2760/18622 , C12N2760/18651 , C12N2760/18652 , C12N2760/18662 , C12N2760/20243 , C12N2760/20251 , C12N2760/20252 , C12N2760/20262
摘要： Methods for producing infectious, non-segmented, negative-stranded RNA viruses of the Order Mononegavirales are provided that involve coexpression of a viral cDNA along with essential viral proteins, N, P, and L in a host cell transiently transfected with an expression vector encoding an RNA polymerase. In alternate methods, after the host cell is transfected with a viral cDNA expression vector and one or more vectors encoding the RNA polymerase, N protein, P protein, and L protein, the host cell is exposed to an effective heat shock under conditions sufficient to increase recovery of the recombinant virus. In other alternate embodiments, the host cells are transferred after viral rescue begins into co-culture with a plaque expansion cell, typically a monolayer of expansion cells, and the assembled infectious, non-segmented, negative-stranded RNA virus is recovered from the co-culture. Also provided within the invention are compositions for producing infectious, non-segmented, negative-stranded RNA virus of the Order Mononegavirales, recombinant viruses produced using the foregoing methods and compositions, and immunogenic compositions and methods employing the recombinant viruses. In additional embodiments, the methods and compositions of the invention are employed to produce growth- or replication-defective non-segmented negative-stranded RNA viruses and subviral particles.
摘要翻译：提供了用于产生单宁属病毒感染性，非分段的负链RNA病毒的方法，其涉及病毒cDNA与基本病毒蛋白N，P和L共表达在用表达载体编码瞬时转染的宿主细胞中 RNA聚合酶。在替代方法中，在用病毒cDNA表达载体和编码RNA聚合酶，N蛋白，P蛋白和L蛋白的一种或多种载体转染宿主细胞后，将宿主细胞暴露于有效的热休克，增加重组病毒的恢复。在其它替代实施方案中，宿主细胞在病毒救治开始与噬菌斑扩增细胞（通常为单层扩增细胞）共培养之后被转移，并且从共同体中回收组装的感染性，非分段的负链RNA病毒 -文化。在本发明中还提供了用于产生单宁属（Mononegavirales）的感染性，非分段的负链RNA病毒的组合物，使用前述方法和组合物产生的重组病毒，以及使用重组病毒的免疫原性组合物和方法。在另外的实施方案中，本发明的方法和组合物用于产生生长或复制缺陷型非分段负链RNA病毒和亚病毒颗粒。

2. 发明申请

US20070218078A1 Synergistic Attenuation of Vesicular Stomatitis Virus, Vectors Thereof and Immunogenic Compositions Thereof 有权
标题翻译：水泡性口炎病毒及其载体及其免疫原性组成的协同衰减
公开(公告)号：US20070218078A1
公开(公告)日：2007-09-20
申请号：US11547236
申请日：2005-04-05
申请人： David Clarke , Roger Hendry , Stephen Udem , Christopher Parks
发明人： David Clarke , Roger Hendry , Stephen Udem , Christopher Parks
IPC分类号： A61K39/12 , C12N7/01
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K2039/5254 , A61K2039/5256 , A61K2039/545 , C12N7/00 , C12N15/86 , C12N2740/16234 , C12N2760/20222 , C12N2760/20243 , C12N2760/20264 , Y02A50/466
摘要： The present invention broadly relates to the synergistic attenuation of vesicular stomatitis virus (VSV). More particularly, the invention relates to the identification of combined mutation classes which synergistically attenuate the pathogenicity of VSV vectors in mammals and immunogenic compositions thereof.
摘要翻译：本发明广泛涉及囊泡性口炎病毒（VSV）的协同衰减。更具体地说，本发明涉及协同减弱哺乳动物中VSV载体的致病性的组合突变类型的鉴定及其免疫原性组合物。

3. 发明申请

US20050089985A1 Rescue of canine distemper virus from cdna 审中-公开
标题翻译：从cdna拯救犬瘟热病毒
公开(公告)号：US20050089985A1
公开(公告)日：2005-04-28
申请号：US10312052
申请日：2001-06-22
申请人： Christopher Parks , Mohinderjit Sidhu , Pramila Walpita , Gerald Kovacs , Stephen Udem
发明人： Christopher Parks , Mohinderjit Sidhu , Pramila Walpita , Gerald Kovacs , Stephen Udem
IPC分类号： C12N15/09 , A61K35/76 , A61K39/00 , A61K39/175 , A61P31/12 , A61P31/14 , C07K14/12 , C07K14/13 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/02 , C12N15/40 , C12N15/45 , C12N15/86
CPC分类号： C12N7/00 , A61K2039/5256 , C07K14/005 , C12N2760/18422 , C12N2760/18451
摘要： This invention relates to a method for recombinantly producing via rescue canine distemper virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the canine distemper virus as an attenuated and/or infectious viruses. The recombinant viruses can be prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and rearrangements, in the genome can be obtained.
摘要翻译：本发明涉及通过救援犬瘟热病毒，非分段，负义的单链RNA病毒和由其形成的免疫原性组合重组生产的方法。另外的实施方案涉及生产犬瘟热病毒作为减毒和/或感染性病毒的方法。可以从cDNA克隆制备重组病毒，因此，可以获得在基因组中具有确定的变化（包括核苷酸/多核苷酸缺失，插入，取代和重排）的病毒。

4. 发明申请

US20050119471A1 Sequence analysis of the tetravalent rotavirus vaccine 失效
标题翻译：四价轮状病毒疫苗的序列分析
公开(公告)号：US20050119471A1
公开(公告)日：2005-06-02
申请号：US10505818
申请日：2003-02-19
申请人： Deborah Buonagurio , Alice Georgiu , Robert Lerch , Bruce Mason , Shridhara Murthy , Ruth Rappaport , Mohinder Sidhu , Stephen Udem , Timothy Zamb
发明人： Deborah Buonagurio , Alice Georgiu , Robert Lerch , Bruce Mason , Shridhara Murthy , Ruth Rappaport , Mohinder Sidhu , Stephen Udem , Timothy Zamb
IPC分类号： A61K39/00 , C07K14/14 , C12N7/04 , C12Q1/70 , C07H21/04 , C12N15/86
CPC分类号： C12N7/00 , A61K39/00 , A61K2039/525 , A61K2039/5254 , C07K14/005 , C12N2720/12322 , C12N2720/12362 , C12Q1/701
摘要： Isolated nucleic acid molecules comprising a gene segment from a rhesus rotavirus (RRV) or from one of three rhesus: human reassortant viruses are disclosed, including isolated nucleic acid molecules having a sequence selected from the group consisting of: SEQ ID NO: 1-14, inclusive, and isolated nucleic acid molecules encoding a protein having a sequence selected from the group consisting of SEQ ID NO: 15-28, inclusive, as well as variants of the isolated nucleic acid molecules.
摘要翻译：公开了分离的核酸分子，其包含来自恒河猴轮状病毒（RRV）的基因片段或三种恒河猴中的一种：人重配病毒，包括具有选自以下的序列的分离的核酸分子：SEQ ID NO：1-14 和包含编码具有选自SEQ ID NO：15-28的序列的蛋白质的分离的核酸分子，以及分离的核酸分子的变体。

5. 发明申请

US20070253972A1 Rescue of mumps virus from cDNA 审中-公开
标题翻译：从拯救腮腺炎病毒的cDNA
公开(公告)号：US20070253972A1
公开(公告)日：2007-11-01
申请号：US11823364
申请日：2007-06-27
申请人： David Clarke , J. Johnson , Mohinderjit Sidhu , Stephen Udem
发明人： David Clarke , J. Johnson , Mohinderjit Sidhu , Stephen Udem
IPC分类号： A61K39/00 , C07H21/04 , C12N15/00 , C12N7/00
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/165 , A61K2039/5254 , A61K2039/70 , C12N7/00 , C12N15/86 , C12N2760/18722 , C12N2760/18734 , C12N2760/18743 , C12N2760/18751 , C12N2760/18761 , C12N2840/20 , C12N2840/203
摘要： This invention relates to a method for recombinantly producing, via rescue of mumps virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the mumps virus as an attenuated and/or infectious virus. The recombinant viruses are prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotide/polynucleotide deletions, insertions, substitutions and re-arrangements, in the place of the genome are obtained.
摘要翻译：本发明涉及通过拯救腮腺炎病毒，非分段的，负义的单链RNA病毒和由其形成的免疫原性组合来重组生产的方法。另外的实施方案涉及产生腮腺炎病毒作为减毒和/或感染性病毒的方法。重组病毒由cDNA克隆制备，因此，获得了具有定义的变化（包括核苷酸/多核苷酸缺失，插入，取代和重新排列）的病毒代替基因组的病毒。

6. 发明申请

US20070134200A1 Immunogenic composition and methods 审中-公开
标题翻译：免疫原性组成和方法
公开(公告)号：US20070134200A1
公开(公告)日：2007-06-14
申请号：US10550313
申请日：2004-03-23
申请人： John Eldridge , Zimra Israel , Michael Egan , Stephen Udem
发明人： John Eldridge , Zimra Israel , Michael Egan , Stephen Udem
IPC分类号： A61K48/00 , A61K38/20 , A61K38/19
CPC分类号： C12N15/86 , A61K38/19 , A61K38/193 , A61K38/2013 , A61K38/2086 , A61K39/12 , A61K39/21 , A61K2039/5256 , A61K2039/53 , A61K2039/54 , A61K2039/543 , A61K2039/545 , A61K2039/55538 , C12N2740/15034 , C12N2740/16034 , C12N2740/16134 , C12N2740/16234 , C12N2760/20243 , C12N2840/20 , A61K2300/00
摘要： A method of inducing an antigen-specific immune response in a mammalian subject includes the steps of administering to the subject an effective amount of a first composition comprising a DNA plasmid comprising a DNA sequence encoding an antigen under the control of regulatory sequences directing expression thereof in a mammalian or vertebrate cell. The method also includes administering to the subject an effective amount of a second composition comprising a recombinant vesicular stomatitis virus (rVSV) comprising a nucleic acid sequence encoding the antigen under the control of regulatory sequences directing expression thereof in the mammalian or vertebrate cell. The rVSV is in one embodiment replication competent. Kits for use in immunizations and therapeutic treatments of disease include the components and instructions for practice of this method.
摘要翻译：在哺乳动物受试者中诱导抗原特异性免疫应答的方法包括以下步骤：向受试者施用有效量的第一组合物，所述第一组合物包含DNA质粒，所述DNA质粒包含编码抗原的DNA序列，所述DNA序列在引导其表达的调控序列的控制下哺乳动物或脊椎动物细胞。该方法还包括向受试者施用有效量的包含重组水泡性口炎病毒（rVSV）的第二组合物，其包含编码抗原的核酸序列，所述核酸序列在引导其在哺乳动物或脊椎动物细胞中的表达的调节序列的控制下。在一个实施方案中，rVSV具有复制能力。用于免疫接种和疾病治疗的试剂盒包括用于实施该方法的组分和说明书。

7. 发明申请

US20070128222A1 Fusogenic, self-propagating blebs as immunogenic compositions 失效
标题翻译：融合蛋白，自发性泡泡作为免疫原性组合物
公开(公告)号：US20070128222A1
公开(公告)日：2007-06-07
申请号：US10559429
申请日：2004-06-02
申请人： Gerald Kovacs , Xiaoyan Mo , Nikolaos Vasilakis , Sangeeta Bhargava , Timothy Zamb , Stephen Udem
发明人： Gerald Kovacs , Xiaoyan Mo , Nikolaos Vasilakis , Sangeeta Bhargava , Timothy Zamb , Stephen Udem
IPC分类号： A61K39/145 , A61K48/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/155 , A61K2039/5256 , A61K2039/543 , C12N15/86 , C12N2760/18522 , C12N2760/18622 , C12N2760/18634 , C12N2770/36122 , C12N2770/36143
摘要： Self-propagating, fusogenic blebs are produced from cells infected with a population of Venezuelan Equine Encephalitis virus replicon particles (VRP). The self-propagating, fusogenic nature of the blebs is derived from expression of heterologous genes encoding viral fusion proteins that are incorporated into the replication defective replicon particles. The resulting blebs can be harvested from supernatants of cells displaying severe cytopathic effects. The blebs are used to make immunogenic compositions and devise methods of immunizing mammals against paramyxoviruses such as parainfluenza virus type 3.
摘要翻译：自体繁殖的融合泡沫是由感染了委内瑞拉马脑炎病毒复制子粒子（VRP）的细胞产生的。泡沫的自发性，融合性质源于编码病毒融合蛋白的异源基因的表达，其被并入到复制缺陷复制子颗粒中。可以从显示严重细胞病变效应的细胞的上清液中收获所得到的水泡。用来制备免疫原性组合物并设计免疫哺乳动物抗副粘病毒如3型副流感病毒的方法。

8. 发明申请

US20070258997A1 Rescue of mumps virus from CDNA 审中-公开
标题翻译：从CDNA拯救腮腺炎病毒
公开(公告)号：US20070258997A1
公开(公告)日：2007-11-08
申请号：US11823309
申请日：2007-06-27
申请人： David Clarke , J. Johnson , Mohinderjit Sidhu , Stephen Udem
发明人： David Clarke , J. Johnson , Mohinderjit Sidhu , Stephen Udem
IPC分类号： A61K39/165 , A61K39/295
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/165 , A61K2039/5254 , A61K2039/70 , C12N7/00 , C12N15/86 , C12N2760/18722 , C12N2760/18734 , C12N2760/18743 , C12N2760/18751 , C12N2760/18761 , C12N2840/20 , C12N2840/203
摘要： This invention relates to a method for recombinantly producing, via rescue of mumps virus, a nonsegmented, negative-sense, single-stranded RNA virus, and immunogenic compositions formed therefrom. Additional embodiments relate to methods of producing the mumps virus as an attenuated and/or infectious virus. The recombinant viruses are prepared from cDNA clones, and, accordingly, viruses having defined changes, including nucleotidelpoly/nucleotide deletions, insertions, substitutions and re-arrangements, in the place of the genome are obtained.
摘要翻译：本发明涉及通过拯救腮腺炎病毒，非分段的，负义的单链RNA病毒和由其形成的免疫原性组合来重组生产的方法。另外的实施方案涉及产生腮腺炎病毒作为减毒和/或感染性病毒的方法。从cDNA克隆制备重组病毒，因此获得了具有定义的改变（包括核苷酸/核苷酸缺失，插入，取代和重新排列）的病毒代替基因组的病毒。

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