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    • 6. 发明申请
    • Characterising polypeptides
    • 表征多肽
    • US20070009960A1
    • 2007-01-11
    • US10565563
    • 2004-07-22
    • Jurgen SchaferChristian HamonJosef SchwarzChristopher Pearce
    • Jurgen SchaferChristian HamonJosef SchwarzChristopher Pearce
    • G01N33/53C12Q1/37G01N33/00
    • G01N33/6851C07D403/14C07D413/14C07K1/13G01N33/533G01N33/6848
    • Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte; wherein the light absorbing label comprises a fluorophore moiety, and wherein prior to detecting by mass spectrometry, the analyte is selected for detection on the basis of its fluorophore moiety.
    • 提供了一种通过基质辅助激光解吸电离(MALDI)质谱鉴定分析物的方法,该方法包括:(a)用预吸收光的光吸收标记物以预定的频率标记分析物,形成标记的 分析物; (b)将标记的分析物包埋在由吸收光的至少一种化合物形成的基质中,以形成嵌入的标记分析物; (c)通过将嵌入的标记分析物暴露于具有预定频率的光来解吸,形成解吸附的分析物; 和(d)通过质谱检测解吸附的分析物来表征分析物; 其中所述光吸收标签包含荧光团部分,并且其中在通过质谱法检测之前,根据其荧光团部分选择所述分析物进行检测。