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1. 发明授权

US12123874B2 Reagent compounds, compositions, kits, and methods for amplified assays 有权
公开(公告)号：US12123874B2
公开(公告)日：2024-10-22
申请号：US16318793
申请日：2017-07-18
申请人： CELL IDX, INC. , THE UNIVERSITY OF CHICAGO
发明人： David A. Schwartz , Stephen J. Kron
IPC分类号： G01N33/547 , C12N9/04 , C12N9/08 , C12N9/16 , C12Q1/6804 , C12Q1/682 , G01N33/533 , G01N33/535 , G01N33/543
CPC分类号： G01N33/547 , C12N9/0006 , C12N9/0065 , C12N9/16 , C12Q1/6804 , C12Q1/682 , G01N33/533 , G01N33/535 , G01N33/54353 , C12Y101/03004 , C12Y111/01007 , C12Y301/03001 , C12Q1/682 , C12Q2531/101 , C12Q2531/125 , C12Q2543/101 , C12Q1/6804 , C12Q2531/101 , C12Q2531/125 , C12Q2543/101
摘要： The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids. Also provided are detectable antibodies specific for the bridging antigens, kits comprising the reagent compounds and antibody and oligonucleotide reagents, methods of signal amplification using the compounds and reagents of the disclosure, methods of preparation of the compounds and reagents, and compositions comprising the compounds and reagents.

2. 发明授权

US11867696B2 Antigen-coupled immunoreagents 有权
公开(公告)号：US11867696B2
公开(公告)日：2024-01-09
申请号：US15017626
申请日：2016-02-06
申请人： CELL IDX, INC.
发明人： David A. Schwartz
IPC分类号： G01N33/00 , G01N33/535 , G01N33/574 , G01N33/533 , C07K16/44 , C07K16/00 , G01N33/543
CPC分类号： G01N33/5743 , C07K16/00 , C07K16/44 , G01N33/533 , G01N33/535 , G01N33/54306 , G01N33/574 , G01N2474/20 , G01N2800/60
摘要： The present disclosure provides high-performance immunoreagents for use in a variety of immunologic assays and other related techniques. The immunoreagents comprise a primary antibody and a bridging antigen, wherein the bridging antigen is recognized by a detectable secondary antibody with high affinity. Also provided are compositions comprising panels of immunoreagents specific for multiple different target antigens and compositions comprising pairs of primary immunoreagents and their complementary detectable secondary antibodies. The paired primary immunoreagents and secondary antibodies are useful in a variety of immunologic assays, particularly in highly multiplexed assays, where the structure of the bridging antigen is varied in tandem with variation in the detectable secondary antibody, such that a multiplicity of immunoreagents are provided that are capable of simultaneously detecting a multiplicity of target antigens in a single assay. Also provided are kits comprising the immunoreagents, methods of immunologic assay using the immunoreagents of the disclosure, and methods of preparation of the immunoreagents.

3. 发明申请

US20220170073A1 METHODS AND/OR USE OF OLIGONUCLEOTIDE CONJUGATES FOR ASSAYS AND FLOW CYTOMETRY DETECTIONS 有权
公开(公告)号：US20220170073A1
公开(公告)日：2022-06-02
申请号：US17369771
申请日：2021-07-07
申请人： The University of Chicago , EMD Millipore Corporation
发明人： David A. Schwartz , Jimmy Williams , Xinfang Zhao , Chunfang Zhao , William B. Busa , Stephen J. Kron , Amy Catherine Flor
IPC分类号： C12Q1/6804 , C07H21/00 , C12Q1/6834
摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

4. 发明申请

US20130344508A1 Methods and/or Use of Oligonucleotide Conjugates for Suppressing Background Due to Cross-Hybridization 有权
标题翻译：寡核苷酸缀合物用于抑制交叉杂交背景的方法和/或用途
公开(公告)号：US20130344508A1
公开(公告)日：2013-12-26
申请号：US13898015
申请日：2013-05-20
申请人： The University of Chicago , SoluLink, Inc.
发明人： David A. Schwartz , Jimmy Williams , Xinfang Zhao , Stephen J. Kron , Amy Catherine Flor
IPC分类号： G01N33/53
CPC分类号： G01N33/5306 , C12Q1/6804 , C12Q1/6848 , G01N33/547
摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and detections and related systems and/or kits for suppressing background due to cross-hybridization. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.
摘要翻译：本公开涉及用于测定和检测的寡核苷酸缀合物和由于交叉杂交抑制背景的相关系统和/或试剂盒的方法和/或用途。某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。本文公开了各种其它实施例，应用等。

5. 发明授权

US09222936B2 Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization 有权
标题翻译：用于抑制由于交叉杂交引起的背景的寡核苷酸共轭物的方法和/或用途
公开(公告)号：US09222936B2
公开(公告)日：2015-12-29
申请号：US13898015
申请日：2013-05-20
申请人： SoluLink, Inc. , The University of Chicago
发明人： David A. Schwartz , Jimmy Williams , Xinfang Zhao , Stephen J. Kron , Amy Catherine Flor
IPC分类号： G01N33/53 , C12Q1/68 , G01N33/547
CPC分类号： G01N33/5306 , C12Q1/6804 , C12Q1/6848 , G01N33/547
摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and detections and related systems and/or kits for suppressing background due to cross-hybridization. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.
摘要翻译：本公开涉及用于测定和检测的寡核苷酸缀合物和由于交叉杂交抑制背景的相关系统和/或试剂盒的方法和/或用途。某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。本文公开了各种其它实施例，应用等。

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