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1. 发明授权

US5358931A Interaction of thermal hysteresis proteins with cells and cell membranes and associated applications 失效
标题翻译：热滞后蛋白与细胞和细胞膜的相互作用及相关应用
公开(公告)号：US5358931A
公开(公告)日：1994-10-25
申请号：US4919
申请日：1993-01-15
申请人： Boris Rubinsky , Arthur L. Devries , Amir Arav
发明人： Boris Rubinsky , Arthur L. Devries , Amir Arav
IPC分类号： A01N1/02 , A61K47/48 , C07K14/46 , A61K37/00 , A61K37/10
CPC分类号： A01N1/02 , A01N1/0221 , A61K47/48 , C07K14/461
摘要： A newly discovered property of thermal hysteresis proteins is the interaction of these proteins with cell membranes and thus with cells themselves, protecting cells and their membranes from damage which they would otherwise suffer upon exposure to non-physiological conditions such as temperature abnormalities, including both hyperthermic, hypothermic and sub-freezing temperatures. Improved rates of cell viability are observed over a wide range of conditions which do not involve ice formation, including temperatures above the freezing range as well as temperatures below the freezing range but in vitrification conditions. Heretofore the only known property of these proteins was their ability to interact with ice crystals. In conditions in which ice crystals are formed, it is further discovered that use of the proteins with human cells at the concentrations in which they naturally occur in the source organisms results in aggravating the injury to the cells rather than reducing it, but that the injury is lessened, and the survival rate improved, by using low concentrations. The proteins thus offer benefits in the preservation and improved viability of cell suspensions, tissues and whole organs. The proteins are further discovered to have the ability to block ion channels in mammalian cell membranes, thereby providing a further utility in the treatment of disease conditions.
摘要翻译：新发现的热滞后蛋白质是这些蛋白质与细胞膜的相互作用，因此与细胞本身相互作用，保护细胞和它们的膜免受其它暴露于非生理条件（如温度异常），低温和低温温度。在不涉及冰形成的宽范围条件下观察到细胞存活率的改善，包括高于冷冻范围的温度以及低于冰点范围的温度，但在玻璃化条件下。迄今为止，这些蛋白质唯一已知的性质是它们与冰晶相互作用的能力。在形成冰晶的条件下，进一步发现，使用蛋白质与人类细胞以其天然存在于源生物体中的浓度导致加重对细胞的损伤而不是降低细胞损伤，但是损伤通过使用低浓度降低生存率。因此，蛋白质在细胞悬浮液，组织和整个器官的保存和改善的活力方面提供益处。进一步发现蛋白质具有阻止哺乳动物细胞膜中的离子通道的能力，从而提供治疗疾病状况的另外的效用。

2. 发明授权

US07331186B2 Changing the temperature of a liquid sample and a receptacle useful therefor 有权
标题翻译：改变液体样品和有用的容器的温度
公开(公告)号：US07331186B2
公开(公告)日：2008-02-19
申请号：US10519222
申请日：2003-02-10
申请人： Amir Arav
发明人： Amir Arav
IPC分类号： F25D25/00 , F25D17/02
CPC分类号： A01N1/02 , A01N1/0257 , B01L7/00 , B01L7/02 , B01L7/50 , B01L2300/0832 , B01L2300/0854 , B01L2300/185 , F25D17/02
摘要： Disclosed is a method of changing the temperature of a liquid sample, comprising: providing a receptacle having inner and outer walls defining an annular portion therebetween for receiving therein a liquid sample, inserting said liquid sample, at a first temperature, into said annular portion, and exposing said receptacle to a second temperature different from said first temperature. Further disclosed are a receptacle useful in the above method and a chamber useful for performing said method.
摘要翻译：公开了一种改变液体样品的温度的方法，包括：提供具有内壁和外壁的插座，其在其间限定环形部分，以在其中接收液体样品，将所述液体样品在第一温度下插入到所述环形部分中，以及将所述容器暴露于与所述第一温度不同的第二温度。进一步公开的是用于上述方法的容器和用于执行所述方法的室。

3. 发明申请

US20120276581A1 METHOD FOR PRESERVING CANCELLOUS BONE SAMPLES AND PRESERVED CANCELLOUS BONE TISSUE 审中-公开
标题翻译：保留取消骨髓样品和保留骨髓组织的方法
公开(公告)号：US20120276581A1
公开(公告)日：2012-11-01
申请号：US13520721
申请日：2011-01-06
申请人： Amir Arav , Victor Rzepakovsky , Isachar Norman , Olga Shneerson , Yehudit Natan
发明人： Amir Arav , Victor Rzepakovsky , Isachar Norman , Olga Shneerson , Yehudit Natan
IPC分类号： C12N5/077 , C12Q1/04
CPC分类号： A01N1/0221 , A01N1/0284 , A01N1/0289
摘要： The claimed subject matter is based on the finding that it is possible to cryopreserve cancellous bone tissue with viable cells (after reconstitution by either thawing or rehydration), the viability after reconstitution being comparable to that of a fresh sample (without freezing) and of beneficial value for use in, for example, transplantation. Thus, provided is a method for cryopreserving a cancellous bone sample. The cryopreserved cancellous bone sample can be in dry form, for example, lyophilized. Also provided is a cryopreserved, in dry form, bone sample and the use of a cryopreserved bone sample; a method for identifying such cryopreserved cancellous bone tissue and uses thereof.
摘要翻译：所要求保护的主题是基于以下发现：有可能用活细胞冷冻保存松质骨组织（在通过解冻或再水合重组后），重建后的存活力与新鲜样品（不冷冻）相当并且是有益的用于例如移植的价值。因此，提供了一种用于冷冻保存松质骨样品的方法。冷冻保存的松质骨样品可以是干燥形式，例如冻干的。还提供了一种冷冻保存的干燥形式的骨样品和使用低温保存的骨样品; 用于鉴定这种冷冻保藏的松质骨组织的方法及其用途。

4. 发明授权

US07659111B2 Method for freezing viable cells 失效
标题翻译：冻存活细胞的方法
公开(公告)号：US07659111B2
公开(公告)日：2010-02-09
申请号：US10488414
申请日：2002-09-05
申请人： Uri Meir , Yoram Amsalem , Yehudit Natan , Amir Arav
发明人： Uri Meir , Yoram Amsalem , Yehudit Natan , Amir Arav
IPC分类号： C12M1/02 , F25D13/06
CPC分类号： A01N1/02 , A01N1/0257 , A01N1/0278 , B01L3/5082 , B01L7/5255 , B01L2300/1894 , F25D3/02 , F26B5/06 , Y10S435/809
摘要： A method and a device for improved freezing of biological containing living cells is disclosed. Directional cooling coupled with agitation, preferably rotation, of the vessel containing the biological sample allows for the freezing of large volume samples at much greater rates than before. Whether a sample is large or not, the greater thermal homogeneity during the freezing process as well as the resulting crystal morphology yield a frozen sample which is exceptionally suited for lyophilization.
摘要翻译：公开了一种改善生物体内活细胞冻结的方法和装置。与包含生物样品的容器的搅动，优选旋转的定向冷却允许以比以前更高的速率冷冻大体积样品。无论样品是否大，冷冻过程中的热均匀性以及所得的晶体形态都会产生非常适合冷冻干燥的冷冻样品。

5. 发明申请

US20080185386A1 Changing the temperature of a liquid sample and a receptacle useful therefor 审中-公开
标题翻译：改变液体样品和有用的容器的温度
公开(公告)号：US20080185386A1
公开(公告)日：2008-08-07
申请号：US12007946
申请日：2008-01-17
申请人： Amir Arav
发明人： Amir Arav
IPC分类号： F28D15/00
CPC分类号： A01N1/02 , A01N1/0257 , B01L7/00 , B01L7/02 , B01L7/50 , B01L2300/0832 , B01L2300/0854 , B01L2300/185 , F25D17/02
摘要： Disclosed is a method of changing the temperature of a liquid sample, comprising: providing a receptacle having inner and outer walls defining an annular portion therebetween for receiving therein a liquid sample, inserting said liquid sample, at a first temperature, into said annular portion, and exposing said receptacle to a second temperature different from said first temperature, Further disclosed are a receptacle useful in the above method and a chamber useful for performing said method.
摘要翻译：公开了一种改变液体样品的温度的方法，包括：提供具有内壁和外壁的插座，其在其间限定环形部分，以在其中接收液体样品，将所述液体样品在第一温度下插入到所述环形部分中，并将所述容器暴露于与所述第一温度不同的第二温度。还公开了可用于上述方法的容器和用于执行所述方法的室。

6. 发明申请

US20070099172A1 Methods and device for freezing and thawing biological samples 审中-公开
标题翻译：冷冻和解冻生物样品的方法和装置
公开(公告)号：US20070099172A1
公开(公告)日：2007-05-03
申请号：US11633431
申请日：2006-12-05
申请人： Amir Arav , Victor Rzepakovsky , Uri Meir
发明人： Amir Arav , Victor Rzepakovsky , Uri Meir
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0257 , A01N1/0268 , A01N1/0284
摘要： A method for changing the temperature of a sample from an initial temperature via an intermediate temperature to a final temperature, one of the initial and final temperatures being above the freezing point of the sample and the other being below the freezing point is provided. The method is for changing the temperature of a sample having minimal dimension in each of two mutually perpendicular cross-sections exceeding 0.5 centimeters, and at least one of the cross-sections having an outer zone and an inner zone.
摘要翻译：提供了将样品从初始温度经由中间温度改变到最终温度的方法，其中初始温度和最终温度之一高于样品的凝固点，另一个温度低于凝固点。该方法是用于在两个相互垂直的横截面中的每一个超过0.5厘米的范围内改变具有最小尺寸的样品的温度，并且至少一个横截面具有外部区域和内部区域。

7. 发明申请

US20050175975A1 Methods of preserving functionality of an organ, preserving fertility of a patient undergoing a treatment expected to cause sterility and assuring a supply of viable gametes for future use 失效
标题翻译：维持器官功能的方法，保持经历预期会导致不育的治疗的患者的生育能力，并确保为将来使用提供可行的配子
公开(公告)号：US20050175975A1
公开(公告)日：2005-08-11
申请号：US11102728
申请日：2005-04-11
申请人： Amir Arav , Yehudit Natan , Saar Yavin , Uri Meir , Udi Damari
发明人： Amir Arav , Yehudit Natan , Saar Yavin , Uri Meir , Udi Damari
IPC分类号： A01N1/02
CPC分类号： A01N1/0284 , A01N1/02
摘要： A method of preserving functionality of an organ. The method includes removing a whole organ and associated vasculature, cryo-preserving the whole organ, allowing a period of time to elapse, thawing the whole organ including vasculature and introducing the whole organ into a recipient so that the organ is supplied with blood by vasculature belonging to the recipient. Further disclosed is a method of preserving fertility of a patient undergoing a treatment expected to cause sterility. The method include removing a whole gonadal organ from the patient, cryo-preserving the whole gonadal organ,conducting the treatment and waiting for an effect thereof to subside, thawing the whole gonadal organ and introducing the whole gonadal organ where it is supplied with blood by the vasculature system of the patient. Further disclosed is a method of assuring a supply of viable gametes for future use, the method includes removing a whole gonadal organ from a patient, cryo-preserving the whole gonadal organ, waiting until viable gametes are desired by the patient, thawing the organ and introducing the gonadal organ into the patient so that it is supplied with blood by a vasculature system belonging to the patient.
摘要翻译：一种维持器官功能的方法。该方法包括去除整个器官和相关的脉管系统，冷冻保存整个器官，允许一段时间过去，解冻整个器官，包括脉管系统，并将整个器官引入受体，以便通过脉管系统为器官提供血液属于收件人。进一步公开的是保持经历预期导致不育的治疗的患者的生育能力的方法。该方法包括从患者身上清除整个性腺器官，冷冻保存整个性腺器官，进行治疗并等待其效果，使整个性腺器官融化，解冻，并将其中所有供血的性腺器官引入患者的脉管系统。进一步公开了一种确保供应可行配子以供将来使用的方法，该方法包括从患者中去除整个性腺器官，冷冻保存整个性腺器官，等待患者期望可食性配子，解冻器官和将性腺器官引入患者，使其由属于患者的脉管系统供给血液。

8. 发明授权

US5873254A Device and methods for multigradient directional cooling and warming of biological samples 失效
标题翻译：生物样品多向定向冷却和升温的装置和方法
公开(公告)号：US5873254A
公开(公告)日：1999-02-23
申请号：US709350
申请日：1996-09-06
申请人： Amir Arav
发明人： Amir Arav
IPC分类号： A01N1/02 , B01L7/00 , F25D3/11 , F25D13/06
CPC分类号： B01L7/52 , A01N1/02 , A01N1/0257 , A01N1/0284 , F25D13/06 , F25D3/11
摘要： A device for controlled freezing and warming of a biological sample, and freezing and thawing protocols for which the device is well suited. The device establishes a laterally varying thermal gradient and provides a mechanism for moving the sample along the thermal gradient at a controlled rate of speed. The sample is moved along the thermal gradient at a rate of speed that provides a variable cooling rate or a variable warming rate in accordance with the appropriate protocol. The device also allows continuous seeding of the sample through the freezing process at the exact freezing point of the solution. Real time monitoring and video imaging of the freezing process enable fine tuning of the thermodynamic parameters for improved control.
摘要翻译：用于生物样品的受控冷冻和升温的装置以及该装置非常适合的冷冻和解冻协议。该装置建立横向变化的热梯度，并提供用于以受控的速度沿着热梯度移动样品的机构。样品以速度速度沿着热梯度移动，其速率根据适当的方案提供可变的冷却速率或可变的升温速率。该装置还允许在溶液的确切冰点处通过冷冻过程连续接种样品。冷冻过程的实时监控和视频成像可以精确调整热力学参数，从而改善控制。

9. 发明授权

US08580487B2 Methods of preserving functionality of an organ, preserving fertility of a patient undergoing a treatment expected to cause sterility and assuring a supply of viable gametes for future use 失效
公开(公告)号：US08580487B2
公开(公告)日：2013-11-12
申请号：US11102728
申请日：2005-04-11
申请人： Amir Arav , Yehudit Natan , Saar Yavin , Uri Meir , Udi Damari
发明人： Amir Arav , Yehudit Natan , Saar Yavin , Uri Meir , Udi Damari
IPC分类号： A01N1/02
CPC分类号： A01N1/0284 , A01N1/02
摘要： A method of preserving functionality of an organ. The method includes removing a whole organ and associated vasculature, cryo-preserving the whole organ, allowing a period of time to elapse, thawing the whole organ including vasculature and introducing the whole organ into a recipient so that the organ is supplied with blood by vasculature belonging to the recipient. Further disclosed is a method of preserving fertility of a patient undergoing a treatment expected to cause sterility. The method include removing a whole gonadal organ from the patient, cryo-preserving the whole gonadal organ, conducting the treatment and waiting for an effect thereof to subside, thawing the whole gonadal organ and introducing the whole gonadal organ where it is supplied with blood by the vasculature system of the patient. Further disclosed is a method of assuring a supply of viable gametes for future use, the method includes removing a whole gonadal organ from a patient, cryo-preserving the whole gonadal organ, waiting until viable gametes are desired by the patient, thawing the organ and introducing the gonadal organ into the patient so that it is supplied with blood by a vasculature system belonging to the patient.

10. 发明申请

US20050223716A1 Changing the temperature of a liquid sample and a receptacle useful therefor 有权
标题翻译：改变液体样品和有用的容器的温度
公开(公告)号：US20050223716A1
公开(公告)日：2005-10-13
申请号：US10519222
申请日：2003-02-10
申请人： Amir Arav
发明人： Amir Arav
IPC分类号： A01N1/02 , B01L7/00 , B01L7/02 , F25D17/02 , F25D25/00
CPC分类号： A01N1/02 , A01N1/0257 , B01L7/00 , B01L7/02 , B01L7/50 , B01L2300/0832 , B01L2300/0854 , B01L2300/185 , F25D17/02
摘要： Disclosed is a method of changing the temperature of a liquid sample, comprising: providing a receptacle having inner and outer walls defining an annular portion therebetween for receiving therein a liquid sample, inserting said liquid sample, at a first temperature, into said annular portion, and exposing said receptacle to a second temperature different from said first temperature. Further disclosed are a receptacle useful in the above method and a chamber useful for performing said method.
摘要翻译：公开了一种改变液体样品的温度的方法，包括：提供具有内壁和外壁的插座，其在其间限定环形部分，以在其中接收液体样品，将所述液体样品在第一温度下插入到所述环形部分中，以及将所述容器暴露于与所述第一温度不同的第二温度。进一步公开的是用于上述方法的容器和用于执行所述方法的室。

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