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1. 发明授权

US07445898B2 Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity 有权
标题翻译：用标记的探针和3'至5'核酸外切酶活性进行定量扩增
公开(公告)号：US07445898B2
公开(公告)日：2008-11-04
申请号：US11097463
申请日：2005-03-31
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译：本发明提供了进行定量扩增反应的方法和试剂盒。该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。

2. 发明申请

US20100159447A1 QUANTITATIVE AMPLIFICATION WITH A LABELED PROBE AND 3' TO 5' EXONUCLEASE ACTIVITY 有权
标题翻译：具有标记探针和3'至5'外切酶活性的定量放大
公开(公告)号：US20100159447A1
公开(公告)日：2010-06-24
申请号：US12264200
申请日：2008-11-03
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译：本发明提供了进行定量扩增反应的方法和试剂盒。该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。

3. 发明授权

US08426132B2 Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity 有权
标题翻译：用标记的探针和3'至5'核酸外切酶活性进行定量扩增
公开(公告)号：US08426132B2
公开(公告)日：2013-04-23
申请号：US12264200
申请日：2008-11-03
申请人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
发明人： Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q2561/101 , C12Q2525/161 , C12Q2521/101 , C12Q2537/143 , C12Q2527/143
摘要： This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
摘要翻译：本发明提供了进行定量扩增反应的方法和试剂盒。该方法采用聚合酶和具有切割探针的3'寡核苷酸的3'至5'核酸外切酶活性的酶。

4. 发明授权

US08367376B2 Compositions with polymerase activity 有权
公开(公告)号：US08367376B2
公开(公告)日：2013-02-05
申请号：US12494217
申请日：2009-06-29
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
IPC分类号： C12N9/12 , C12P19/34
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.

5. 发明申请

US20090269829A1 NOVEL COMPOSITIONS WITH POLYMERASE ACTIVITY 有权
标题翻译：新型组合物与聚合酶活性
公开(公告)号：US20090269829A1
公开(公告)日：2009-10-29
申请号：US12494217
申请日：2009-06-29
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
IPC分类号： C12N9/10 , C07H21/04 , C12N15/63
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.
摘要翻译：本发明提供具有聚合酶活性的新型组合物和使用该组合物的方法。

6. 发明授权

US07560260B2 Compositions with polymerase activity 有权
标题翻译：具有聚合酶活性的组合物
公开(公告)号：US07560260B2
公开(公告)日：2009-07-14
申请号：US10627582
申请日：2003-07-25
申请人： Peter B. Vander Horn , Yan Wang
发明人： Peter B. Vander Horn , Yan Wang
IPC分类号： C12N9/12 , C12P19/34
CPC分类号： C12N9/1252 , C07K2319/00 , C12N9/1241 , C12Y207/07007
摘要： The invention provides novel compositions with polymerase activity and methods of using the compositions.
摘要翻译：本发明提供具有聚合酶活性的新型组合物和使用该组合物的方法。

7. 发明授权

US08021611B2 Automated micro-volume assay system 有权
标题翻译：自动微量测定系统
公开(公告)号：US08021611B2
公开(公告)日：2011-09-20
申请号：US11401699
申请日：2006-04-10
申请人： David J. Roach , Tom W. Yang , Roger A. O'Neill , Robert T. Loder, Jr. , Peter B. Vander Horn
发明人： David J. Roach , Tom W. Yang , Roger A. O'Neill , Robert T. Loder, Jr. , Peter B. Vander Horn
IPC分类号： B01L9/06 , B01L9/00
CPC分类号： F16L41/00 , G01N35/04 , G01N35/1065 , G01N2035/1034 , G01N2035/1039 , G01N2035/1051 , G01N2035/1062 , Y10T137/0318 , Y10T137/85938 , Y10T436/11 , Y10T436/119163 , Y10T436/2575
摘要： An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation and immobilization station where reactions inside the capillaries take place and a detector station where photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run. Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format.
摘要翻译：描述了用于放置用于毛细管内部的材料测定的材料的站的自动测定系统和用于操纵毛细管的自动化夹具。该系统包括分离和固定站，其中毛细管内部发生反应，并且检测到来自毛细血管反应的光摄取的检测器站。来自毛细血管的光摄取可以显示为线图或类似于熟悉的Western凝胶印迹的假凝胶图像的列。自动化控制系统具有用户界面，通过该界面，操作员可以选择运行协议并定义要在协议运行中使用的样本和试剂的位置。在设置之后，控制系统将使自动化系统执行协议，然后以选定的显示格式显示结果。

8. 发明授权

US08632975B2 Nucleotide transient binding for sequencing methods 有权
标题翻译：测序方法的核苷酸瞬变结合
公开(公告)号：US08632975B2
公开(公告)日：2014-01-21
申请号：US12790760
申请日：2010-05-28
申请人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
发明人： Peter B. Vander Horn , Cheng-Yao Chen , Guobin Luo , Michael Previte , Jamshid Temirov , Theo Nikiforov , Zhaohui Zhou , Hongye Sun , Yufang Wang , Stefanie Yukiko Nishimura , Hongyi Wang , Marian Peris , Barnett B. Rosenblum , Michael Phelan
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/68 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6869 , C12Q1/6872 , C12Y207/07007 , G01N2500/00 , C12Q2565/632 , C12Q2563/137 , C12Q2563/107 , C12Q2565/30 , C12Q2537/157
摘要： Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
摘要翻译：本文提供了用于聚合酶依赖性核苷酸瞬变结合方法的组合物和系统。该方法可用于推导模板核酸分子和单核苷酸多态性（SNP）分析的序列。该方法依赖于与非互补核苷酸相比，互补核苷酸的聚合酶瞬时结合时间更长的事实。标记的核苷酸以模板依赖的方式瞬时结合聚合酶，但不包括在内。所述方法在允许互补或非互补核苷酸与聚合酶短暂结合并且抑制核苷酸掺入的任何反应条件下进行。

9. 发明申请

US20120213667A1 AUTOMATED MICRO-VOLUME ASSAY SYSTEM 审中-公开
标题翻译：自动微量测定系统
公开(公告)号：US20120213667A1
公开(公告)日：2012-08-23
申请号：US13236316
申请日：2011-09-19
申请人： David J. Roach , Tom W. Yang , Roger A. O'Neill , Robert T. Loder, JR. , Peter B. Vander Horn
发明人： David J. Roach , Tom W. Yang , Roger A. O'Neill , Robert T. Loder, JR. , Peter B. Vander Horn
IPC分类号： G01N35/10
CPC分类号： F16L41/00 , G01N35/04 , G01N35/1065 , G01N2035/1034 , G01N2035/1039 , G01N2035/1051 , G01N2035/1062 , Y10T137/0318 , Y10T137/85938 , Y10T436/11 , Y10T436/119163 , Y10T436/2575
摘要： An automated assay system is described with stations for placement of materials to be used in an assay of materials inside capillaries and an automated gripper for manipulating capillaries. The system includes a separation and immobilization station where reactions inside the capillaries take place and a detector station where photoemissions from the capillary reactions are detected. The photoemissions from the capillaries may be displayed as line graphs or in columns of a pseudo-gel image resembling the familiar Western gel blot. An automated control system has a user interface by which an operator can select a run protocol and define the locations of samples and reagents to be used in the protocol run. Following the setup the control system will cause the automated system to execute the protocol, then display the results in a selected display format.
摘要翻译：描述了用于放置用于毛细管内部的材料测定的材料的站的自动测定系统和用于操纵毛细管的自动化夹具。该系统包括分离和固定站，其中毛细管内部发生反应，并且检测到来自毛细血管反应的光摄取的检测器站。来自毛细血管的光摄取可以显示为线图或类似于熟悉的Western凝胶印迹的假凝胶图像的列。自动化控制系统具有用户界面，通过该界面，操作员可以选择运行协议并定义要在协议运行中使用的样本和试剂的位置。在设置之后，控制系统将使自动化系统执行协议，然后以选定的显示格式显示结果。

10. 发明授权

US06682641B1 Electrophoresis assembly and method of casting electrophoresis gels 失效
标题翻译：电泳组装及电泳凝胶电泳方法
公开(公告)号：US06682641B1
公开(公告)日：2004-01-27
申请号：US09559906
申请日：2000-04-26
申请人： Michael J. Finney , Daniel E. Sullivan , Bruce R. Turner , Alexis Vira , Peter B. Vander Horn , Charles P. Andre , Sean Rubin , Corey Nislow , John R. Linton , William D. Bowers
发明人： Michael J. Finney , Daniel E. Sullivan , Bruce R. Turner , Alexis Vira , Peter B. Vander Horn , Charles P. Andre , Sean Rubin , Corey Nislow , John R. Linton , William D. Bowers
IPC分类号： B01D5702
CPC分类号： G01N27/44704
摘要： The invention provides an electrophoresis cassette to cast electrophoresis gels and to separate and analyze molecular components by electrophoresis. The electrophoresis cassette comprises a top plate assembly, a spacer and a bottom plate. The top plate assembly is seated to the bottom plate with the spacer there between to define a thickness of the electrophoresis cassette and to seal an outer perimeter of the assembly. The top plate assembly includes a cathode reservoir connected to a first terminal end of a central plate, and an anode reservoir connected to a second terminal end of the central plate. When the electrophoresis cassette is assembled, the cathode and anode reservoirs are in alignment with the first and second terminal ends of the central plate to facilitate formation of leak-proof seals between the reservoirs and the assembly components. An embodiment of the invention provides a plurality of sample wells incorporated with the cathode reservoir and constructed of rigid, electrically non-conducting material to provide a hard-well sample loading site for uniform and consistent sample injection. Another embodiment provides a mechanical biasing system incorporated with a cathode reservoir body to bias the assembly components together. The invention also provides a method of casting electrophoresis gels with the electrophoresis cassette provided herein.
摘要翻译：本发明提供了一种电泳盒，用于铸造电泳凝胶，并通过电泳分离和分析分子成分。电泳盒包括顶板组件，间隔件和底板。顶板组件位于底板上，其间具有间隔件，以限定电泳盒的厚度并密封组件的外周。顶板组件包括连接到中心板的第一末端的阴极储存器和连接到中心板的第二终端的阳极储存器。当组装电泳盒时，阴极和阳极储存器与中心板的第一和第二终端对准，以便在储存器和组件部件之间形成防漏密封。本发明的一个实施例提供了多个与阴极储存器结合并由刚性的非导电材料构成的样品阱，以提供用于均匀和一致的样品注入的硬孔样品加载位点。另一个实施例提供了结合有阴极储存器主体以将组件部件偏置在一起的机械偏置系统。本发明还提供了一种使用本文提供的电泳盒来浇铸电泳凝胶的方法。

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