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1. 发明授权

US6072050A Synthetic promoters 失效
公开(公告)号：US6072050A
公开(公告)日：2000-06-06
申请号：US28819
申请日：1998-02-24
申请人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
发明人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
IPC分类号： C12N9/02 , C12N15/29 , C12N15/82 , A01H5/00 , A01H4/00 , C12N15/00
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.

2. 发明授权

US06555673B1 Synthetic promoters 有权
标题翻译：合成促进剂
公开(公告)号：US06555673B1
公开(公告)日：2003-04-29
申请号：US09556163
申请日：2000-04-21
申请人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
发明人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
IPC分类号： C07H2104
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a “TATA to start” sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
摘要翻译：公开了用于增强植物细胞中基因表达的合成元件。这些包括具有含有64％或更高GC含量的“TATA起始”序列的启动子和包含若干OCS结合基序和新侧翼序列的合成上游元件。还公开了上游活化区（UAR），当它们可操作地连接到所述启动子和/或合成上游元件时，可以进一步增加组成型转录活性。特别地，提供了玉米Ubi-1基因的UAR的核苷酸序列，并且其用于表达盒和含有这些启动子元件的载体。进一步提供用这些载体转化的细胞和植物。这些包括在具有35S CaMV启动子的至少一个上游活化区的重组启动子的转录控制下在高水平表达外源性草酸氧化酶基因的转基因向日葵。

3. 发明授权

US06399859B1 Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof 失效
标题翻译：植物尿苷二磷酸 - 葡萄糖脱氢酶基因，蛋白质及其用途
公开(公告)号：US06399859B1
公开(公告)日：2002-06-04
申请号：US08987367
申请日：1997-12-10
申请人： Scott E. Nichols , Kanwarpal S. Dhugga , George W. Singletary , Court A. Saunders , Derrick Witcher , Wesley B. Bruce , Lynne E. Sims , Guihua Lu , Gan-Yuan Zhong
发明人： Scott E. Nichols , Kanwarpal S. Dhugga , George W. Singletary , Court A. Saunders , Derrick Witcher , Wesley B. Bruce , Lynne E. Sims , Guihua Lu , Gan-Yuan Zhong
IPC分类号： C12N1582
CPC分类号： C12N9/0006 , A01K2217/05 , A23K10/30 , C12N15/8242 , C12N15/8245 , C12N15/8261 , C12N15/8286 , Y02A40/146 , Y02A40/162
摘要： Isolated nucleic acid molecules are provided that encode maize UDPGdH variant UDPGdH, and mutant UDPGdH proteins. These nucleic acid molecules can be used to produce transgenic plants having altered quality or quantity of starch. Also provided are vectors capable of expressing such nucleic acid molecules, host cells containing, such vectors, and polypeptides encoded by such nucleic acids.
摘要翻译：提供了编码玉米UDPGdH变体UDPGdH和突变型UDPGdH蛋白质的分离的核酸分子。这些核酸分子可用于产生具有改变的淀粉质量或数量的转基因植物。还提供了能够表达这种核酸分子的载体，含有此类载体的宿主细胞和由该核酸编码的多肽。

4. 发明授权

US06518483B1 Root-preferred promoters and their use 有权
标题翻译：根优选的启动子及其用途
公开(公告)号：US06518483B1
公开(公告)日：2003-02-11
申请号：US09444905
申请日：1999-11-22
申请人： Wesley B. Bruce , Lynne E. Sims
发明人： Wesley B. Bruce , Lynne E. Sims
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8222 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Knox1，Kn1样同源框基因和从其分离的序列的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

5. 发明申请

US20100186113A1 Root-Tip Preferred Promoter from Maize 审中-公开
标题翻译：来自玉米的根尖优选启动子
公开(公告)号：US20100186113A1
公开(公告)日：2010-07-22
申请号：US12622480
申请日：2009-11-20
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims
IPC分类号： C12N15/29 , C12N5/10 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root tip-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自玉米的根尖优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

6. 发明授权

US07268226B2 Maize Cyclo1 gene and promoter 有权
标题翻译：玉米Cyclo1基因和启动子
公开(公告)号：US07268226B2
公开(公告)日：2007-09-11
申请号：US11327727
申请日：2006-01-06
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
IPC分类号： C12N15/63 , C12N15/82 , C12N15/11 , C12N15/00 , C12N5/04 , C12N5/10
CPC分类号： C12N15/8227 , C07K14/415 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。本发明还涉及编码植物环肽的分离的核酸。本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。

7. 发明授权

US07696412B2 Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans 有权
标题翻译：编码具有杀虫活性的新型苏云金芽孢杆菌蛋白的基因
公开(公告)号：US07696412B2
公开(公告)日：2010-04-13
申请号：US10414637
申请日：2003-04-16
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。

8. 发明授权

US07268270B2 Maize Cr1Bio gene promoter and its use to direct root-preferred transgene expression in plants 失效
标题翻译：玉米Cr1Bio基因启动子及其在植物中引导根系优先转基因表达的用途
公开(公告)号：US07268270B2
公开(公告)日：2007-09-11
申请号：US11274768
申请日：2005-11-15
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
IPC分类号： C12N15/82 , C12N5/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8234 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cr1Bio. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method includes stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cr1Bio的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

9. 发明申请

US20110209242A1 Green Tissue-Preferred Promoter from Maize 审中-公开
标题翻译：绿色组织优选启动子从玉米
公开(公告)号：US20110209242A1
公开(公告)日：2011-08-25
申请号：US13028367
申请日：2011-02-16
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims
IPC分类号： C12N15/82 , C07H21/00 , C12N15/63 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C07K14/415 , C12N15/8223 , C12N15/8225
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a green tissue-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自玉米的绿色组织优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

10. 发明授权

US07193135B2 Root-preferred, wound- and insect-inducible 2-oxoglutarate-dependent oxygenase promoter from maize and its use 有权
标题翻译：来自玉米的根本优选，伤口和昆虫诱导型2-氧戊二酸依赖性加氧酶启动子及其用途
公开(公告)号：US07193135B2
公开(公告)日：2007-03-20
申请号：US11299452
申请日：2005-12-12
申请人： Scott Diehn , Albert L. Lu , Billy F. McCutchen , Lynne E. Sims , Kim R. Ward
发明人： Scott Diehn , Albert L. Lu , Billy F. McCutchen , Lynne E. Sims , Kim R. Ward
IPC分类号： C12P7/62 , C12P11/00 , C07H21/04 , A01H1/00
CPC分类号： C12N9/0069 , C07K14/415 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米2-氧戊二酸依赖性加氧酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

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