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1. 发明授权

US07696412B2 Genes encoding novel Bacillus thuringiensis proteins with pesticidal activity against Coleopterans 有权
标题翻译：编码具有杀虫活性的新型苏云金芽孢杆菌蛋白的基因
公开(公告)号：US07696412B2
公开(公告)日：2010-04-13
申请号：US10414637
申请日：2003-04-16
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。

2. 发明授权

US08188036B2 Genes encoding novel proteins with pesticidal activity against coleopterans 有权
标题翻译：编码对鞘翅目具有杀虫活性的新型蛋白质的基因
公开(公告)号：US08188036B2
公开(公告)日：2012-05-29
申请号：US12472997
申请日：2009-05-27
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01N37/18
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编号δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其具有针对鞘翅目害虫的杀虫活性。本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。这些组合物可用于防治害虫，特别是植物害虫的方法。

3. 发明申请

US20090291896A1 GENES ENCODING NOVEL PROTEINS WITH PESTICIDAL ACTIVITY AGAINST COLEOPTERANS 有权
标题翻译：基因编码具有杀虫活性的新型蛋白质
公开(公告)号：US20090291896A1
公开(公告)日：2009-11-26
申请号：US12472997
申请日：2009-05-27
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01N37/18 , C07K7/08 , C07K14/00 , A01P17/00
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，表达盒以及包含本发明核酸的转化微生物和植物的分离的核酸。这些组合物可用于防治害虫，特别是植物害虫的方法。

4. 发明授权

US07605304B2 Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans 有权
标题翻译：编码具有抗鞘翅目杀虫活性的新型苏云金芽孢杆菌蛋白的基因
公开(公告)号：US07605304B2
公开(公告)日：2009-10-20
申请号：US10032717
申请日：2001-10-23
申请人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
发明人： Andre R. Abad , Nicholas B. Duck , Xiang Feng , Ronald D. Flannagan , Theodore W. Kahn , Lynne E. Sims
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的针对鞘翅目害虫的具有杀虫活性的苏云金芽孢杆菌（Bacillus thuringiensis）菌株获得的核酸及其变体和片段。本发明还提供已被修饰以编码具有改善的杀虫活性和/或改变的有害生物特异性的内毒素的诱变的核酸。本发明的具体实施方案提供编码杀虫蛋白的分离的核酸，包含此类核酸的表达盒，以及包含本发明核酸的转化植物和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。

5. 发明申请

US20100186113A1 Root-Tip Preferred Promoter from Maize 审中-公开
标题翻译：来自玉米的根尖优选启动子
公开(公告)号：US20100186113A1
公开(公告)日：2010-07-22
申请号：US12622480
申请日：2009-11-20
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims
IPC分类号： C12N15/29 , C12N5/10 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root tip-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自玉米的根尖优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

6. 发明授权

US07268226B2 Maize Cyclo1 gene and promoter 有权
标题翻译：玉米Cyclo1基因和启动子
公开(公告)号：US07268226B2
公开(公告)日：2007-09-11
申请号：US11327727
申请日：2006-01-06
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
IPC分类号： C12N15/63 , C12N15/82 , C12N15/11 , C12N15/00 , C12N5/04 , C12N5/10
CPC分类号： C12N15/8227 , C07K14/415 , C12N15/8279
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cyclo1. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence. The present invention also relates to isolated nucleic acids encoding plant cyclotides. The invention relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cyclo1的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。本发明还涉及编码植物环肽的分离的核酸。本发明涉及在正义或反义方向上构建编码全部或部分植物环肽的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环肽。

7. 发明授权

US07268270B2 Maize Cr1Bio gene promoter and its use to direct root-preferred transgene expression in plants 失效
标题翻译：玉米Cr1Bio基因启动子及其在植物中引导根系优先转基因表达的用途
公开(公告)号：US07268270B2
公开(公告)日：2007-09-11
申请号：US11274768
申请日：2005-11-15
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims , Kim R. Ward
IPC分类号： C12N15/82 , C12N5/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8234 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Cr1Bio. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method includes stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Cr1Bio的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

8. 发明授权

US06518483B1 Root-preferred promoters and their use 有权
标题翻译：根优选的启动子及其用途
公开(公告)号：US06518483B1
公开(公告)日：2003-02-11
申请号：US09444905
申请日：1999-11-22
申请人： Wesley B. Bruce , Lynne E. Sims
发明人： Wesley B. Bruce , Lynne E. Sims
IPC分类号： A01H500
CPC分类号： C12N15/8216 , C07K14/415 , C12N15/8222 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding Knox1, a Kn1-like homeobox gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Knox1，Kn1样同源框基因和从其分离的序列的基因的根优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

9. 发明授权

US6072050A Synthetic promoters 失效
公开(公告)号：US6072050A
公开(公告)日：2000-06-06
申请号：US28819
申请日：1998-02-24
申请人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
发明人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
IPC分类号： C12N9/02 , C12N15/29 , C12N15/82 , A01H5/00 , A01H4/00 , C12N15/00
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.

10. 发明申请

US20110209242A1 Green Tissue-Preferred Promoter from Maize 审中-公开
标题翻译：绿色组织优选启动子从玉米
公开(公告)号：US20110209242A1
公开(公告)日：2011-08-25
申请号：US13028367
申请日：2011-02-16
申请人： Scott Diehn , Albert L. Lu , Lynne E. Sims
发明人： Scott Diehn , Albert L. Lu , Lynne E. Sims
IPC分类号： C12N15/82 , C07H21/00 , C12N15/63 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C07K14/415 , C12N15/8223 , C12N15/8225
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a green tissue-preferred promoter from maize. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stabling incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于来自玉米的绿色组织优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子可操作地连接的核苷酸序列掺入到植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

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