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1. 发明授权

US06555673B1 Synthetic promoters 有权
标题翻译：合成促进剂
公开(公告)号：US06555673B1
公开(公告)日：2003-04-29
申请号：US09556163
申请日：2000-04-21
申请人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
发明人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
IPC分类号： C07H2104
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a “TATA to start” sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.
摘要翻译：公开了用于增强植物细胞中基因表达的合成元件。这些包括具有含有64％或更高GC含量的“TATA起始”序列的启动子和包含若干OCS结合基序和新侧翼序列的合成上游元件。还公开了上游活化区（UAR），当它们可操作地连接到所述启动子和/或合成上游元件时，可以进一步增加组成型转录活性。特别地，提供了玉米Ubi-1基因的UAR的核苷酸序列，并且其用于表达盒和含有这些启动子元件的载体。进一步提供用这些载体转化的细胞和植物。这些包括在具有35S CaMV启动子的至少一个上游活化区的重组启动子的转录控制下在高水平表达外源性草酸氧化酶基因的转基因向日葵。

2. 发明授权

US6072050A Synthetic promoters 失效
公开(公告)号：US6072050A
公开(公告)日：2000-06-06
申请号：US28819
申请日：1998-02-24
申请人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
发明人： Benjamin A. Bowen , Wesley B. Bruce , Guihua Lu , Lynne E. Sims , Laura A. Tagliani
IPC分类号： C12N9/02 , C12N15/29 , C12N15/82 , A01H5/00 , A01H4/00 , C12N15/00
CPC分类号： C12N9/0008 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8226 , C12N15/8279
摘要： Synthetic elements for enhancing expression of genes in plant cells are disclosed. These include a promoter with a "TATA to start" sequence containing 64% or greater GC content and an synthetic upstream element incorporating several OCS binding motifs and novel flanking sequences. Upstream activating regions (UARs) are also disclosed that can further increase the constitutive transcriptional activity when they are operably linked to said promoter and/or the synthetic upstream element. In particular, the nucleotide sequence of the UAR of the maize Ubi-1 gene is provided and its use in expression cassettes and vectors containing these promoter elements. Cells and plants transformed with these vectors are further provided. These include a transgenic sunflower expressing an exogenous oxalate oxidase gene at a high level under the transcriptional control of a recombinant promoter having at least one upstream activating region of the 35S CaMV promoter.

3. 发明申请

US20110030107A1 COMPOSITIONS AND METHODS FOR GENETIC MODIFICATION OF PLANTS 有权
标题翻译：植物遗传修饰的组合物和方法
公开(公告)号：US20110030107A1
公开(公告)日：2011-02-03
申请号：US12909944
申请日：2010-10-22
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： A01H5/00 , C12N5/04 , A01H5/10
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译：提供了将核苷酸序列靶向整合到植物中的方法。使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。

4. 发明授权

US06528700B1 Targeted manipulation of genes in plants 失效
标题翻译：植物中基因的靶向操作
公开(公告)号：US06528700B1
公开(公告)日：2003-03-04
申请号：US09193612
申请日：1998-11-17
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N1582
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译：提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。该方法包括将嵌合寡核苷酸引入具有中间DNA残基的RNA残基中。 RNA嵌段与植物核苷酸序列同源。嵌合寡核苷酸能折叠形成双链寡核苷酸。嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。

5. 发明授权

US08536420B2 Compositions and methods for genetic modification of plants 有权
标题翻译：植物遗传修饰的组成和方法
公开(公告)号：US08536420B2
公开(公告)日：2013-09-17
申请号：US12184564
申请日：2008-08-01
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译：提供了将核苷酸序列靶向整合到植物中的方法。使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。

6. 发明授权

US08143504B2 Compositions and methods for genetic modification of plants 有权
标题翻译：植物遗传修饰的组成和方法
公开(公告)号：US08143504B2
公开(公告)日：2012-03-27
申请号：US12909944
申请日：2010-10-22
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译：提供了将核苷酸序列靶向整合到植物中的方法。使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。

7. 发明授权

US07102055B1 Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant 有权
标题翻译：将目的核苷酸序列靶向插入植物基因组的组合物和方法
公开(公告)号：US07102055B1
公开(公告)日：2006-09-05
申请号：US09455051
申请日：1999-12-06
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： C12N15/90 , C12N15/82 , C12N15/31 , A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译：提供了将核苷酸序列靶向整合到植物中的方法。使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。

8. 发明申请

US20090093059A1 COMPOSITIONS AND METHODS TO STACK MULTIPLE NUCLEOTIDE SEQUENCES OF INTEREST IN THE GENOME OF A PLANT 有权
标题翻译：在植物基因组中堆叠多个核苷酸序列的组合物和方法
公开(公告)号：US20090093059A1
公开(公告)日：2009-04-09
申请号：US12266016
申请日：2008-11-06
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： C12N15/82 , C12N15/11
CPC分类号： C12N15/8216 , C07K2319/00 , C12N9/00 , C12N15/102 , C12N15/1137 , C12N15/8203 , C12N15/8205 , C12N15/8213 , C12N15/8274 , C12N15/90 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
摘要翻译：提供了用于在植物或植物细胞的基因组中的精确位置堆叠多个核苷酸序列的方法和组合物。具体而言，将包含不同重组位点的感兴趣的核苷酸序列的转移盒导入包含靶位点的植物中。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。转移盒和靶位点被设计为允许核苷酸序列在植物基因组中精确位置的堆叠或排序。

9. 发明申请

US20080320617A1 COMPOSITIONS AND METHODS FOR GENETIC MODIFICATION OF PLANTS 有权
标题翻译：植物遗传修饰的组合物和方法
公开(公告)号：US20080320617A1
公开(公告)日：2008-12-25
申请号：US12184564
申请日：2008-08-01
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , David J. Peterson , Laura A. Tagliani
IPC分类号： A01H5/00
CPC分类号： C12N15/8213 , C07K2319/00 , C12N9/00 , C12N15/8203 , C12N15/8205 , C12N15/90
摘要： Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
摘要翻译：提供了将核苷酸序列靶向整合到植物中的方法。使用包含侧翼为不相同重组位点的感兴趣的核苷酸序列的转移盒转化包含靶位点的植物。靶位点至少包含一组与转移盒上相同的非相同重组位点。通过重组酶交换侧翼为重组位点的核苷酸序列。

10. 发明授权

US06911575B1 Targeted manipulation of genes in plants 失效
标题翻译：植物中基因的靶向操作
公开(公告)号：US06911575B1
公开(公告)日：2005-06-28
申请号：US09579784
申请日：2000-05-26
申请人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
发明人： Christopher L. Baszczynski , Benjamin A. Bowen , John H. Duesing , David J. Peterson , Laura A. Tagliani , Tong Zhu
IPC分类号： C12N15/10 , C12N15/113 , C12N15/82 , C12N15/87
CPC分类号： C12N15/1137 , C12N15/102 , C12N15/8213 , C12N15/8274 , C12N2310/321 , C12N2310/53 , C12N2310/3521
摘要： Methods and compositions for altering a nucleotide sequence of interest in a plant is provided. The method involves introducing a chimeric oligonucleotide into a plant cell, wherein the oligonucleotide comprises at least two intervening blocks of RNA residues with intervening DNA residues. The RNA blocks are homologous to a plant nucleotide sequence. The chimeric oligonucleotide is capable of folding to form a duplex oligonucleotide. The chimeric oligonucleotide is maintained within the nucleus of the plant whereby the alteration is introduced into the target sequences of the plant genome.
摘要翻译：提供了用于改变植物中感兴趣的核苷酸序列的方法和组合物。该方法包括将嵌合寡核苷酸引入植物细胞中，其中所述寡核苷酸包含至少两个具有中间DNA残基的RNA残基中间块。 RNA嵌段与植物核苷酸序列同源。嵌合寡核苷酸能折叠形成双链寡核苷酸。嵌合寡核苷酸保持在植物的细胞核内，从而将改变引入植物基因组的靶序列。

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