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1. 发明授权

US5744342A Protein having heat-resistant malate dehydrogenase activity 失效
标题翻译：具有耐热苹果酸脱氢酶活性的蛋白质
公开(公告)号：US5744342A
公开(公告)日：1998-04-28
申请号：US838418
申请日：1997-04-07
申请人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
IPC分类号： C12N9/04 , C12N15/53
CPC分类号： C12N9/0006
摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
摘要翻译：具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

2. 发明授权

US5686294A Protein having heat-resistant malate dehydrogenase activity 失效
标题翻译：具有耐热苹果酸脱氢酶活性的蛋白质
公开(公告)号：US5686294A
公开(公告)日：1997-11-11
申请号：US270013
申请日：1994-07-01
申请人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
发明人： Atsushi Sogabe , Seiji Takeshima , Kazumi Yamamoto , Shinichi Teshima , Shigenori Emi , Yoshihisa Kawamura
IPC分类号： C12N9/04 , C12N15/53
CPC分类号： C12N9/0006
摘要： A novel protein having a heat-resistant malate dehydrogenase activity, a DNA fragment having a gene encoding said protein, a recombinant vector having said DNA fragment, a transformant transformed with said vector, a method for producing the protein having heat-resistant malate dehydrogenase activity by the use of said transformant, a reagent for GOT determination, comprising the above-mentioned novel protein having a heat-resistant malate dehydrogenase activity and a method for determining GOT activity, which comprises the use of said reagent. According to the present invention, a protein having a heat-resistant malate dehydrogenase activity and having higher purity and superior heat stability can be obtained. In addition, a reagent for GOT determination which is superior in long-term storage can be prepared by the use of the protein having a heat-resistant malate dehydrogenase activity.
摘要翻译：具有耐热苹果酸脱氢酶活性的新型蛋白质，具有编码所述蛋白质的基因的DNA片段，具有所述DNA片段的重组载体，用该载体转化的转化体，具有耐热性苹果酸脱氢酶活性的蛋白质的制造方法通过使用所述转化体，用于GOT测定的试剂，其包含上述具有耐热苹果酸脱氢酶活性的新型蛋白质和确定GOT活性的方法，其包括使用所述试剂。根据本发明，可以得到具有耐热苹果酸脱氢酶活性，纯度高，热稳定性优异的蛋白质。此外，可以通过使用具有耐热苹果酸脱氢酶活性的蛋白质来制备长期保存优良的GOT测定用试剂。

3. 发明授权

US5874078A Reagent composition of glutamate dehydrogenase from pseudomonas 失效
标题翻译：来自假单胞菌的谷氨酸脱氢酶的试剂组成
公开(公告)号：US5874078A
公开(公告)日：1999-02-23
申请号：US582967
申请日：1996-01-04
申请人： Noriyuki Hongo , Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
发明人： Noriyuki Hongo , Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
IPC分类号： C12N9/06 , C12Q1/32 , A61K58/44
CPC分类号： C12Q1/32 , C12N9/0016 , C12Y104/01002 , G01N2333/906 , Y10S435/874
摘要： A novel glutamate dehydrogenase derived from the genus Pseudomonas which is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, is with an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP. A reagent composition for measuring an amount of ammonia characterized in containing a glutamate dehydrogenase which is not activated by ADP and is NADH-dependent, .alpha.-ketoglutaric acid or a salt thereof and NADH; and a reagent composition wherein urease or creatinine deiminase is further added to the above-mentioned reagent composition.
摘要翻译：源于对L-谷氨酸特异性的假单胞菌属的新型谷氨酸脱氢酶需要NAD +和NADH作为辅酶，不作用于NADP +和NADPH，具有最佳pH 10.5-11.5（氧化脱氨基），pH稳定性 5-10（25℃; 20小时），最适温度约60℃（氧化脱氨），分子量约280,000（凝胶过滤）或约41,000（SDS-PAGE），未活化由ADP。一种用于测量氨的量的试剂组合物，其特征在于含有不被ADP活化并且是NADH依赖性的α-酮戊二酸或其盐和NADH的谷氨酸脱氢酶; 以及进一步向上述试剂组合物中加入脲酶或肌酐去酰亚胺酶的试剂组合物。

4. 发明授权

US5773585A Glutamate dehydrogenase from Pseudomonas 失效
标题翻译：来自假单胞菌的谷氨酸脱氢酶
公开(公告)号：US5773585A
公开(公告)日：1998-06-30
申请号：US667357
申请日：1996-06-21
申请人： Noriyuki Hongo , Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
发明人： Noriyuki Hongo , Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
IPC分类号： C12N9/06 , C12Q1/32 , C12N9/02
CPC分类号： C12Q1/32 , C12N9/0016 , C12Y104/01002 , G01N2333/906 , Y10S435/874
摘要： A novel glutamate dehydrogenase is derived from the genus Pseudomonas and is specific to L-glutamic acid, requires NAD.sup.+ and NADH as coenzymes, does not act on NADP.sup.+ and NADPH, has an optimum pH 10.5-11.5 (oxidative deamination), a pH stability of 5-10 (at 25.degree. C.; 20 hours), an optimum temperature of about 60.degree. C. (oxidative deamination) and a molecular weight of about 280,000 (gel filtration) or about 41,000 (SDS-PAGE) and is not activated by ADP.
摘要翻译：新型谷氨酸脱氢酶源自假单胞菌属，对L-谷氨酸特异性，需要NAD +和NADH作为辅酶，不对NADP +和NADPH起作用，具有最佳pH 10.5-11.5（氧化脱氨），pH稳定性 5-10（25℃; 20小时），最适温度约60℃（氧化脱氨），分子量约280,000（凝胶过滤）或约41,000（SDS-PAGE），未活化由ADP。

5. 发明授权

US5821095A Alkaline phosphatase 失效
标题翻译：碱性磷酸酶
公开(公告)号：US5821095A
公开(公告)日：1998-10-13
申请号：US585857
申请日：1996-01-12
申请人： Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
发明人： Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
IPC分类号： C12N9/16 , C12Q1/42 , G01N33/535 , G01N33/58 , C12N9/00
CPC分类号： C12R1/07 , C12N9/16 , C12Q1/42 , G01N33/535 , G01N33/581 , G01N2333/916
摘要： A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.
摘要翻译：描述了一种新的碱性磷酸酶和碱性磷酸酶的生产方法。还描述了用于检测样品中配体的方法和使用碱性磷酸酶分析样品中核酸序列的方法。

6. 发明授权

US5821061A Method and reagent for detecting a ligand in a sample 失效
标题翻译：用于检测样品中配体的方法和试剂
公开(公告)号：US5821061A
公开(公告)日：1998-10-13
申请号：US709434
申请日：1996-09-06
申请人： Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
发明人： Shizuo Hattori , Kazumi Yamamoto , Shinichi Teshima , Yoshihisa Kawamura
IPC分类号： C12N9/16 , C12Q1/42 , G01N33/535 , G01N33/58 , C12Q1/68 , C07H19/00 , C07K1/00 , C12P19/34
CPC分类号： C12R1/07 , C12N9/16 , C12Q1/42 , G01N33/535 , G01N33/581 , G01N2333/916
摘要： A novel alkaline phosphatase and a method for the production of the alkaline phosphatase are described. A method for detecting a ligand in a sample and a method for analyzing the sequence of nucleic acid in a sample using the alkaline phosphatase are also described.
摘要翻译：描述了一种新的碱性磷酸酶和碱性磷酸酶的生产方法。还描述了用于检测样品中配体的方法和使用碱性磷酸酶分析样品中核酸序列的方法。

7. 发明授权

US6162614A Sugar ester derivatives, reagents for measurement of hydrolase activity and methods for measurement of hydrolase activity 失效
标题翻译：糖酯衍生物，水解酶活性测定试剂和水解酶活性测定方法
公开(公告)号：US6162614A
公开(公告)日：2000-12-19
申请号：US975167
申请日：1992-11-12
申请人： Toshio Kurihara , Toshiyuki Nishio , Hisao Yamamoto , Minoru Kamimura , Shinichi Teshima , Tsuneo Hanyu , Shigenori Emi
发明人： Toshio Kurihara , Toshiyuki Nishio , Hisao Yamamoto , Minoru Kamimura , Shinichi Teshima , Tsuneo Hanyu , Shigenori Emi
IPC分类号： C07H15/203 , C12Q1/34 , C12Q1/44
CPC分类号： C07H15/203 , C12Q1/34 , C12Q1/44 , C12Q2334/00 , C12Q2334/10
摘要： A sugar ester derivative of the general formula (I) ##STR1## wherein G stands for a group of the formula (A) ##STR2## or a group of the formula (B) ##STR3## wherein .lambda. means 0 or 1; at least one of R.sub.1 and R.sub.2 means an ester residue of a saturated or unsaturated fatty acid having 1-30 carbon atoms and the other group means hydrogen atom or acetyl group, and R.sub.3 means a group of the formula (C) ##STR4## wherein X means a halogen atom, m means an integer of 0 to 4, Y means hydroxy group, an alkoxy group, a carboxyl group or sulfonic acid group, n means 0 or 1 and Z means nitro group or nitrovinyl group and its use.The sugar ester derivative of the general formula (I) is useful as a substrate for the measurement of lipase or esterase activities, and the reagents and the methods for measuring lipase or esterase activities which comprise said sugar ester derivative as the substrate are excellent in terms of reproducibility and sensitivity and enable the measurement of lipase or esterase activities in accordance with rate-assay method by an easy procedure.
摘要翻译：通式（I）的糖酯衍生物，其中G表示式（A）的基团或式（B）的基团，其中λ表示0或1; R 1和R 2中的至少一个表示具有1-30个碳原子的饱和或不饱和脂肪酸的酯残基，另一个基团是指氢原子或乙酰基，R 3表示式（C）的基团，其中X表示卤素原子，m表示0〜4的整数，Y表示羟基，烷氧基，羧基或磺酸基，n表示0或1，Z表示硝基或硝基乙烯基及其用途。通式（I）的糖酯衍生物可用作测定脂肪酶或酯酶活性的底物，并且包含所述糖酯衍生物作为底物的试剂和测定脂肪酶或酯酶活性的方法是优异的的重现性和灵敏度，并且能够通过简单的方法根据速率测定方法测量脂肪酶或酯酶活性。

8. 发明授权

US06455684B1 Insitu assay of substance in biological sample using labeled probe 失效
标题翻译：使用标记探针对生物样品中的物质进行原位测定
公开(公告)号：US06455684B1
公开(公告)日：2002-09-24
申请号：US09967361
申请日：2001-09-28
申请人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
发明人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
IPC分类号： C07H2102
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biological sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译：一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1为芳族基团，R1为氢或-COCH2COCnF2n + 1和n是1-6的整数，其与选自核酸，核酸结合蛋白，低分子配体和配体（抗体除外）的受体的探针结合，得到荧光复合物，使与生物样品上的目标物质复合，并测定所得荧光复合物，标记的核酸探针和标记的核苷酸的荧光。根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。

9. 发明授权

US06339172B1 Assay of substance in biological sample using labeled probe 失效
标题翻译：使用标记探针对生物样品中的物质进行原位测定
公开(公告)号：US06339172B1
公开(公告)日：2002-01-15
申请号：US09301406
申请日：1999-04-28
申请人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
发明人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
IPC分类号： C07C30900
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprsing reacting a labeled probe with an objective substance on a biological sample, said probe comprsing a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nudeic acid binding potein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biolgical sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译：一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1是芳族基团，R1是氢或-COCH2COCnF2n + 1，n为1-6的整数，其与选自核酸，裸露酸结合蛋白，低分子配体和配体受体（抗体除外）的探针结合，得到荧光复合物，使与目标物质在生物样品上复合并测定所得荧光复合物的荧光，标记的核酸探针和标记的核苷酸。根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。

10. 发明授权

US4889408A Plastic optical fiber less attenuating light and process for producing the same 失效
标题翻译：塑料光纤减弱光线及其制作方法
公开(公告)号：US4889408A
公开(公告)日：1989-12-26
申请号：US926516
申请日：1986-11-04
申请人： Shinichi Teshima
发明人： Shinichi Teshima
IPC分类号： G02B1/04
CPC分类号： G02B1/046 , G02B1/048 , Y10T428/2929 , Y10T428/2967
摘要： A plastic optical fiber comprising a polymer core constituted mainly of methyl methacrylate and a polymer cladding having a lower refractive index than that of the core, characterized in that the core consists of a methyl methacrylate homopolymer or a copolymer constituted of at least 95% by weight of methyl methacrylate and less than 5% by weight of (i) methyl acrylate, (ii) ethyl acrylate, or (iii) a mixture of both acrylates, that the weight-average molecular weight of the core polymer is from 80,000 to 200,000, and that light attenuations through the fiber are up to 250, 130, 80, and 130 dB/Km at wavelengths of 400, 450, 570, and 650 nm, respectively, and a process for producing the same.
摘要翻译：一种塑料光纤，包括主要由甲基丙烯酸甲酯构成的聚合物芯和具有比芯的折射率低的折射率的聚合物包层，其特征在于，芯由甲基丙烯酸甲酯均聚物或由至少95重量％的甲基丙烯酸甲酯和小于5重量％的（i）丙烯酸甲酯，（ii）丙烯酸乙酯，或（iii）两种丙烯酸酯的混合物，芯聚合物的重均分子量为8万至20万，并且通过光纤的光衰减分别在400,450,570和650nm的波长处分别高达250,130,80和130dB / Km，以及其制造方法。

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