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1. 发明授权

US09006515B2 Pollen preferred promoters and methods of use 有权
标题翻译：花粉优选启动子和使用方法
公开(公告)号：US09006515B2
公开(公告)日：2015-04-14
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J Lawit
发明人： Andrew Mark Cigan , Shai J Lawit
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

2. 发明申请

US20130180006A1 Pollen Preferred Promoters and Methods of Use 有权
标题翻译：花粉优选的启动子和使用方法
公开(公告)号：US20130180006A1
公开(公告)日：2013-07-11
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , C12N5/10
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

3. 发明申请

US20130180005A1 Method to Screen Plants for Genetic Elements Inducing Parthenogenesis in Plants 审中-公开
标题翻译：筛选植物遗传元素诱导植物中孤雌生殖的方法
公开(公告)号：US20130180005A1
公开(公告)日：2013-07-11
申请号：US13445276
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： A01H1/06 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8287 , C12N15/8233
摘要： Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.
摘要翻译：提供了缺乏性衍生胚胎的植物群体的组合物和方法。组合物包括编码多核苷酸的抑制盒和产生单性生成的启动子。进一步提供的是孤雌生殖遗传元件，用于防止自我繁殖植物中的有性繁殖。方法包括：利用作为无菌近交维持系统保持的母体胚胎缺陷隐性突变，允许产生对于隐性突变体等位基因是纯合的群体，但是经过补充。方法包括利用通过卵细胞特异性启动子表达的毒素基因，产生显性的无胚胎表型，通过雌性配子不可透过。用驱动解毒剂的卵细胞启动子，花粉消融PTU和用于鉴定转基因种子的种子颜色标记物转化所产生的半合子植物。一代植物50％的女性可育，有种子，当下一代种植时，将产生具有50％活力的转基因种子和50％不能存活的无胚芽种子的植物。

4. 发明授权

US08293975B2 Method for identifying gene function 有权
标题翻译：识别基因功能的方法
公开(公告)号：US08293975B2
公开(公告)日：2012-10-23
申请号：US12061834
申请日：2008-04-03
申请人： Andrew Mark Cigan , Erica Unger
发明人： Andrew Mark Cigan , Erica Unger
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Methods are provided in which a promoter hairpin construct is introduced into a cell to suppress expression of a gene of interest; a change in phenotype of the cell, or of an organism regenerated from the cell, is detected; and the change in phenotype identifies the function of a product of the gene of interest. Also provided are methods for restoring the original phenotype.
摘要翻译：提供了将促进剂发夹构建体导入细胞以抑制目的基因表达的方法; 检测细胞表型或从细胞再生的生物体的变化; 并且表型的变化确定了感兴趣的基因的产物的功能。还提供了恢复原始表型的方法。

5. 发明授权

US08785722B2 Dominant gene suppression transgenes and methods of using same 有权
标题翻译：显性基因抑制转基因及其使用方法
公开(公告)号：US08785722B2
公开(公告)日：2014-07-22
申请号：US12907433
申请日：2010-10-19
申请人： Andrew Mark Cigan , Erica Unger
发明人： Andrew Mark Cigan , Erica Unger
IPC分类号： A01H1/00 , A01H1/02 , C12N15/82 , C12N15/87
CPC分类号： C12N15/8241 , C12N15/8216 , C12N15/8289 , C12N15/829 , G01N33/5097
摘要： Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, methods for testing allelic variants, and methods for repressing transmission of transgenes.
摘要翻译：提供了一对植物，其中补体结构导致在后代中抑制亲本表型。提供了产生和维持这种植物的方法以及所述植物的使用方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。还提供了用于花粉优选表达连接的多核苷酸的调节元件。还提供了用于鉴定基因功能的方法，用于测试等位基因变体的方法以及抑制转基因传播的方法。

6. 发明申请

US20100122367A1 Dominant Gene Suppression Transgenes and Methods of Using Same 有权
标题翻译：优势基因抑制转基因及其使用方法
公开(公告)号：US20100122367A1
公开(公告)日：2010-05-13
申请号：US12688960
申请日：2010-01-18
申请人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
发明人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
IPC分类号： A01H5/00 , C12N15/82 , C12N5/10 , A01H1/02
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.
摘要翻译：提供了一对植物，其中补体结构导致在后代中抑制亲本表型。提供了产生和维持这种植物的方法和使用所述植物的方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。还提供了用于维持纯合隐性病症和抑制转基因传播的方法。

7. 发明申请

US20140338070A1 METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT 有权
标题翻译：方法和组合在工厂中进行集中整合
公开(公告)号：US20140338070A1
公开(公告)日：2014-11-13
申请号：US14236597
申请日：2012-07-18
申请人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael W. Lassner , Zhongsen Li , Zhan-Bin Liu , Leszek A. Lyznik , David J. Peterson , Christopher Jay Scelonge
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael W. Lassner , Zhongsen Li , Zhan-Bin Liu , Leszek A. Lyznik , David J. Peterson , Christopher Jay Scelonge
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
摘要翻译：提供组合物和方法用于将目的多核苷酸序列靶向整合到植物或植物细胞的基因组中。方法和组合物使用核酸内切酶和内切核酸酶的识别位点与位点特异性重组位点/重组酶组合，以提供用于在植物，植物细胞或种子的基因组内建立靶位点的有效系统。一旦建立了这样的靶位点，就可以采用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。

8. 发明申请

US20120011614A1 Dominant Gene Suppression Transgenes and Methods of Using Same 审中-公开
标题翻译：优势基因抑制转基因及其使用方法
公开(公告)号：US20120011614A1
公开(公告)日：2012-01-12
申请号：US13237575
申请日：2011-09-20
申请人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
发明人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
IPC分类号： A01H1/02 , C12N5/04 , C12N15/63 , C12Q1/02 , C12N15/113 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.
摘要翻译：提供了一对植物，其中补体结构导致在后代中抑制亲本表型。提供了产生和维持这种植物的方法和使用所述植物的方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。还提供了用于维持纯合隐性病症和抑制转基因传播的方法。

9. 发明授权

US08067667B2 Dominant gene suppression transgenes and methods of using same 有权
标题翻译：显性基因抑制转基因及其使用方法
公开(公告)号：US08067667B2
公开(公告)日：2011-11-29
申请号：US12688960
申请日：2010-01-18
申请人： Andrew Mark Cigan , Erica Unger
发明人： Andrew Mark Cigan , Erica Unger
IPC分类号： A01H1/00 , A01H1/02 , C12N15/82 , C12N15/87
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of such plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.
摘要翻译：提供了一对植物，其中补体结构导致在后代中抑制亲本表型。提供了产生和维持这种植物和使用这种植物的方法的方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。还提供了用于维持纯合隐性病症和抑制转基因传播的方法。

10. 发明授权

US07696405B2 Dominant gene suppression transgenes and methods of using same 有权
标题翻译：显性基因抑制转基因及其使用方法
公开(公告)号：US07696405B2
公开(公告)日：2010-04-13
申请号：US11014071
申请日：2004-12-16
申请人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
发明人： Andrew Mark Cigan , Timothy W. Fox , Howard Hershey , Erica Unger , Yongzhong Wu
IPC分类号： A01H1/00 , A01H1/02 , C12N15/82 , C12N15/87
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of plants generated or maintained by the methods, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, and methods for repressing transmission of transgenes.
摘要翻译：提供了一对植物，其中补体结构导致在后代中抑制亲本表型。提供了产生和维持这种植物的方法，以及通过该方法产生或维持的植物的使用方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。还提供了用于花粉优选表达连接的多核苷酸的调节元件。还提供用于鉴定基因功能的方法以及抑制转基因传播的方法。

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