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1. 发明申请

US20120266324A1 Self-Reproducing Hybrid Plants 审中-公开
标题翻译：自我复制杂交植物
公开(公告)号：US20120266324A1
公开(公告)日：2012-10-18
申请号：US13445419
申请日：2012-04-12
申请人： SHAI J. LAWIT
发明人： SHAI J. LAWIT
IPC分类号： A01H1/02 , C12N15/63 , C07H21/04 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8287 , A01H1/02 , A01H1/08 , C07K14/415 , C12N15/8218
摘要： Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.
摘要翻译：提供了生产自我再现混合植物的组合物和方法。组合物包括编码多核苷酸和启动子的抑制盒，其导致MiMe二倍体配子表型组合物和抑制盒和表达盒，其用于在受精受精卵中的亲本二倍体配子的基因组消除。所述方法涉及将包含负责产生MiMe二倍体配子表型的第一抑制盒的第一植物和表达活性CENH3突变体的第一表达盒与包含降低野生型CENH3水平的第二抑制盒的第二植物杂交，第二表达盒包含在胚珠中特异性表达CENH3的多核苷酸。所产生的后代植物的自体受精导致雌性二倍体基因组在受精卵中的消除和胚乳的正常发育。另外提供的是通过本发明的方法生产的植物和种子。

2. 发明授权

US09006515B2 Pollen preferred promoters and methods of use 有权
标题翻译：花粉优选启动子和使用方法
公开(公告)号：US09006515B2
公开(公告)日：2015-04-14
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J Lawit
发明人： Andrew Mark Cigan , Shai J Lawit
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

3. 发明申请

US20130180009A1 Compositions and Methods for Expression of a Sequence in a Reproductive Tissue of a Plant 审中-公开
标题翻译：在植物生殖组织中表达序列的组合物和方法
公开(公告)号：US20130180009A1
公开(公告)日：2013-07-11
申请号：US13445440
申请日：2012-04-12
申请人： MARC C. ALBERTSEN , MARK A. CHAMBERLIN , TIMOTHY W. FOX , SHAI J. LAWIT , BRIAN R. LOVELAND
发明人： MARC C. ALBERTSEN , MARK A. CHAMBERLIN , TIMOTHY W. FOX , SHAI J. LAWIT , BRIAN R. LOVELAND
IPC分类号： C12N15/82
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8234 , C12N15/8263 , C12N15/829
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include promoter sequences with direct expression in an egg cell or embryonic cell-preferred manner. Such compositions find use in, for example, a method for expressing a heterologous nucleotide sequence in a plant; detection of specific cell types in the ovule and targeted ablation of specific cell types.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括在卵细胞中直接表达的启动子序列或胚胎细胞优选的方式。这样的组合物可用于例如在植物中表达异源核苷酸序列的方法; 检测胚珠中的特定细胞类型和特定细胞类型的靶向消融。

4. 发明申请

US20130180010A1 Methods and Compositions for Modulating Expression or Activity of a RKD Polypeptide in a Plant 审中-公开
公开(公告)号：US20130180010A1
公开(公告)日：2013-07-11
申请号：US13445447
申请日：2012-04-12
申请人： Mark A. Chamberlin , Shai J. Lawit
发明人： Mark A. Chamberlin , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , A01H5/00 , C12N15/63 , C12N5/10
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8261 , C12N15/8287
摘要： Methods and compositions are provided to increase the activity/level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity/level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.

5. 发明授权

US07557266B2 Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize D9 gene and methods of use 失效
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子及其使用方法
公开(公告)号：US07557266B2
公开(公告)日：2009-07-07
申请号：US11736615
申请日：2007-04-18
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： A01H5/00 , C12N5/14 , C12N15/63 , C07H21/04
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。

6. 发明申请

US20130180008A1 Ovule Specific Promoter and Methods of Use 审中-公开
标题翻译： Ovule特异性启动子和使用方法
公开(公告)号：US20130180008A1
公开(公告)日：2013-07-11
申请号：US13445426
申请日：2012-04-12
申请人： MARK A. CHAMBERLIN , SHAI J. LAWIT
发明人： MARK A. CHAMBERLIN , SHAI J. LAWIT
IPC分类号： C12N15/82
CPC分类号： C12N15/8233 , C12N15/8281 , C12N15/8286 , C12N15/8289
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for an Arabidopsis thaliana (AT SVL3) promoter. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括拟南芥（AT SVL3）启动子的核苷酸序列。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

7. 发明申请

US20130180006A1 Pollen Preferred Promoters and Methods of Use 有权
标题翻译：花粉优选的启动子和使用方法
公开(公告)号：US20130180006A1
公开(公告)日：2013-07-11
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , C12N5/10
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

8. 发明申请

US20120005786A1 Isolated Polynucleotide Molecules Corresponding to Mutant and Wild-Type Alleles of the Maize D9 Gene and Methods of Use 审中-公开
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子和使用方法
公开(公告)号：US20120005786A1
公开(公告)日：2012-01-05
申请号：US13206081
申请日：2011-08-09
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： A01H1/00 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants by decreasing or increasing plant height, altering stem and/or root growth, and involving the use of these isolated polynucleotide molecules, isolated polypeptides and transformed plants, seeds and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了通过降低或增加植物高度，改变茎和/或根生长以及涉及使用这些分离的多核苷酸分子，分离的多肽和转化的植物，种子和细胞来修饰植物生长的方法。

9. 发明申请

US20090172841A1 Isolated Polynucleotide Molecules Corresponding to Mutant and Wild-Type Alleles of the Maize D9 Gene and Methods of Use 审中-公开
标题翻译：对应于玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子和使用方法
公开(公告)号：US20090172841A1
公开(公告)日：2009-07-02
申请号：US12392169
申请日：2009-02-25
申请人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
发明人： Shai J. Lawit , Suman Kundu , Aragula G. Rao , Dwight T. Tomes
IPC分类号： C12N15/82 , C07K14/00
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/827 , C12N15/8297 , Y02A40/146
摘要： The invention provides isolated polynucleotide molecules encoding mutant and wild-type alleles of the maize D9 gene. The invention further provides methods for modifying the growth of plants involving the use of these isolated polynucleotide molecules, isolated polypeptides, and transformed plants, seeds, and cells.
摘要翻译：本发明提供编码玉米D9基因的突变体和野生型等位基因的分离的多核苷酸分子。本发明还提供了修饰涉及使用这些分离的多核苷酸分子，分离的多肽以及转化的植物，种子和细胞的植物生长的方法。

10. 发明授权

US09175301B2 Use of dimerization domain component stacks to modulate plant architecture 有权
标题翻译：使用二聚化结构域组件来调节植物结构
公开(公告)号：US09175301B2
公开(公告)日：2015-11-03
申请号：US12837553
申请日：2010-07-16
申请人： Shai J Lawit , Dwight T Tomes
发明人： Shai J Lawit , Dwight T Tomes
IPC分类号： C12N15/82 , C07H21/04 , C12N5/10 , C07K14/415
CPC分类号： C12N15/8261 , C07C31/08 , C07K14/415 , C12N15/8242 , C12N15/8255 , C12N15/8269 , C12N15/827 , C12N15/8293 , C12N15/8297 , C12P7/06 , Y02A40/146
摘要： This invention provides means for altering the harvest index of crop plants by modulating the expression of transgenic genes using dimerization domain and component stacks, thereby modulating plant architecture. The transgene/dimerization domain stacks are provided in a single transformation vector unit and are used to modulate plant growth, yield, and harvest index in plants.
摘要翻译：本发明提供了通过使用二聚化域和组分堆栈调节转基因基因的表达来改变作物植物收获指数的方法，从而调节植物结构。提供转基因/二聚化结构域的单个转化载体单位，并用于调节植物的植物生长，产量和收获指数。

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