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1. 发明授权

US5346818A Method for the conjugative transfer of mobilizable vectors for E. coli to gram-positive bacteria and vectors suitable for use in such a method 失效
标题翻译：将用于大肠杆菌的可动员载体共轭转移到革兰氏阳性细菌和适用于这种方法的载体的方法
公开(公告)号：US5346818A
公开(公告)日：1994-09-13
申请号：US447139
申请日：1989-12-08
申请人： Andreas Schafer , Jorn Kalinowski , Alfred Puhler
发明人： Andreas Schafer , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N15/09 , C12N1/21 , C12N15/74 , C12N15/77 , C12N15/00 , C12N15/63
CPC分类号： C12N15/74 , C12N15/77
摘要： The present invention relates to a method of effecting the conjugative transfer of a mobilizable vector from cells of an E. coli mobilizer strain to gram-positive bacterial cells. The invention also relates to vectors suitable for use in such a method.
摘要翻译：本发明涉及一种将可移动载体从大肠杆菌移动剂菌株的细胞共轭转移到革兰氏阳性细菌细胞的方法。本发明还涉及适用于这种方法的载体。

2. 发明授权

US5516669A Transposon consisting of a fragment of the R plasmid pCxM82B, test vectors containing this transposon, and methods of mutagenesis 失效
标题翻译：由R质粒pCxM82B的片段组成的转座子，含有该转座子的测试载体和诱变方法
公开(公告)号：US5516669A
公开(公告)日：1996-05-14
申请号：US181164
申请日：1994-01-13
申请人： Friedrich Kassing , Andreas Schafer , Jorn Kalinowski , Alfred Puhler
发明人： Friedrich Kassing , Andreas Schafer , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N1/21 , C12N15/09 , C12N15/70 , C12N15/74 , C12N15/77 , C12R1/19 , C12N15/52 , C12N15/63
CPC分类号： C12N15/77 , C12N15/74
摘要： The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this transposon, and to methods for the mutagenesis of gram-positive bacteria by means of the transfer of these vectors and the generation of auxotrophic mutants.
摘要翻译：本发明涉及一种转座子，其由来自棒状杆菌DSM 5021的R质粒pCxM82B的片段组成，含有该转座子的可移动的非自身转移载体，以及通过以下方法诱变革兰氏阳性细菌的方法：这些载体的转移和营养缺陷突变体的产生。

3. 发明申请

US20050196848A1 Process for the fermentative production of L-amino acids by attenuation of the poxB gene 审中-公开
标题翻译：通过痘痘基因的减毒发酵生产L-氨基酸的方法
公开(公告)号：US20050196848A1
公开(公告)日：2005-09-08
申请号：US11111831
申请日：2005-04-22
申请人： Nicole Dusch , Brigitte Bathe , Jorn Kalinowski , Alfred Puhler , Bettina Mockel , Anke Weissenborn , Walter Pfefferle
发明人： Nicole Dusch , Brigitte Bathe , Jorn Kalinowski , Alfred Puhler , Bettina Mockel , Anke Weissenborn , Walter Pfefferle
IPC分类号： C12N15/09 , C12N1/21 , C12N9/02 , C12N15/31 , C12N15/53 , C12P13/08 , C12R1/15 , C12Q1/68 , C07H21/04 , C12N1/20 , C12N9/10 , C12N15/74
CPC分类号： C12N9/0008
摘要： An isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids by attenuation of the poxB gene.
摘要翻译：一种分离的多核苷酸，其含有多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同。 2，b）编码多肽的多核苷酸，其含有与SEQ ID No：1的氨基酸序列至少70％相同的氨基酸序列。 2）c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）多核苷酸序列的至少15个连续碱基的多核苷酸，以及发酵生产L - 氨基酸通过减少poxB基因。

4. 发明申请

US20050100927A1 Nucleotide sequence encoding the dapC gene and process for the production of L-lysine 有权
标题翻译：编码dapC基因的核苷酸序列和生产L-赖氨酸的方法
公开(公告)号：US20050100927A1
公开(公告)日：2005-05-12
申请号：US10745684
申请日：2003-12-29
申请人： Bettina Mockel , Anke Weissenborn , Walter Pfefferle , Michael Hartmann , Jorn Kalinowski , Alfred Puhler
发明人： Bettina Mockel , Anke Weissenborn , Walter Pfefferle , Michael Hartmann , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N15/31 , C12N15/54 , C12P13/08 , C12Q1/68 , C12R1/13
CPC分类号： C12P13/08 , C12N9/1096
摘要： The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.
摘要翻译：本发明涉及来自棒状细菌的分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码含有根据SEQ ID No.3所述的氨基酸序列的多肽的多核苷酸至少70％相同。 2，b）编码多肽的多核苷酸，其含有与SEQ ID NO：1的氨基酸序列至少70％相同的氨基酸序列。 2）c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及d）发酵生产的方法的L-氨基酸，特别是L-赖氨酸。

5. 发明授权

US06787334B1 Process for the preparation of pantothenic acid by amplification of nucleotide sequences which code for the ketopantoate reductase 有权
标题翻译：通过扩增编码酮聚糖还原酶的核苷酸序列来制备泛酸的方法
公开(公告)号：US06787334B1
公开(公告)日：2004-09-07
申请号：US09680310
申请日：2000-10-06
申请人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
发明人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
IPC分类号： C12P2106
CPC分类号： C12N9/0008 , C12P7/42
摘要： The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
摘要翻译：本发明涉及通过扩增编码酮戊酸还原酶，特别是panE基因的核苷酸序列来单独地或彼此组合并任选地另外添加ilvC来制备和改善D-泛酸产生微生物的方法基因，含有这些核苷酸序列的微生物，以及D-泛酸的制备方法，其包括这些微生物的发酵，在培养基中或微生物细胞中浓缩泛酸，以及分离D-泛酸。

6. 发明授权

US06184006B2 Method for the fermentative production of D-pantothenic acid using strains of the family enterobacteriaceae 失效
标题翻译：使用肠杆菌科菌株发酵生产D-泛酸的方法
公开(公告)号：US06184006B2
公开(公告)日：2001-02-06
申请号：US09318739
申请日：1999-05-26
申请人： Mechthild Rieping , Georg Thierbach , Walter Pfefferle , Nicole Dusch , Jorn Kalinowski , Alfred Puhler
发明人： Mechthild Rieping , Georg Thierbach , Walter Pfefferle , Nicole Dusch , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12P1300
CPC分类号： C12N15/52 , C12N9/88 , C12P13/02
摘要： A method for the production of D-pantothenic acid by the fermentation of microorganisms of the family Enterobacteriacae producing D-pantothenic acid which is characterized in that strains are used which a) Contain the plasmid pFV31 and/or pFV202 and that b) A panD gene and optionally other nucleotide sequences coding for aspartate-1-decarboxylase are enhanced, especially overexpressed in these microorganisms, c) The pantothenic acid is enriched in the medium or in the cells of the microorganisms and d) The pantothenic acid formed is isolated.
摘要翻译：通过发酵产生D-泛酸家族肠杆菌的方法生产D-泛酸的方法，其特征在于使用以下菌株：a）含有质粒pFV31和/或pFV202，以及b）PanD基因和任选地编码天冬氨酸-1-脱羧酶的其他核苷酸序列增强，特别是在这些微生物中过表达，c）泛酸在培养基中或微生物细胞中富集，d）分离形成的泛酸。

7. 发明申请

US20100190653A1 Nucleotide sequences encoding alanine racemase from coryneform 有权
标题翻译：从棒状杆菌编码丙氨酸消旋酶的核苷酸序列
公开(公告)号：US20100190653A1
公开(公告)日：2010-07-29
申请号：US10304743
申请日：2002-11-27
申请人： Andreas Tauch , Michael Binder , Walter Pfefferle , Georg Thierbach , Jorn Kalinowski , Alfred Puhler
发明人： Andreas Tauch , Michael Binder , Walter Pfefferle , Georg Thierbach , Jorn Kalinowski , Alfred Puhler
IPC分类号： C40B30/04 , C07H21/04 , C12N1/20 , C12N1/21 , C12N15/74 , C12P13/04 , C12P13/06 , C12P13/08
CPC分类号： C12N9/90 , C12P13/06 , C12P13/08 , C12Y501/01001
摘要： The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译：本发明涉及具有编码alr基因的多核苷酸序列的分离的多核苷酸，以及具有其中alr基因以减毒形式存在的棒状细胞宿主细菌的宿主 - 载体系统和至少携带alr基因的载体至SEQ ID No 1，以及使用包含根据本发明的序列的多核苷酸作为杂交探针。

8. 发明申请

US20060205046A1 NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE 失效
标题翻译：编号为ZWA1基因的核苷酸序列
公开(公告)号：US20060205046A1
公开(公告)日：2006-09-14
申请号：US11419529
申请日：2006-05-22
申请人： Bettina Mockel , Walter Pfefferle , Achim Marx , Jorn Kalinowski , Brigitte Bathe , Alfred Puhler
发明人： Bettina Mockel , Walter Pfefferle , Achim Marx , Jorn Kalinowski , Brigitte Bathe , Alfred Puhler
IPC分类号： C12P13/08 , C12N15/74 , C12N1/21
CPC分类号： C07K14/34 , C12N9/00 , C12P13/08
摘要： Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
摘要翻译：本文公开了一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％相同程度的氨基酸序列c）与a）和 b）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。

9. 发明授权

US06777229B1 Plasmids from Corynebacterium glutamicum and use thereof 有权
标题翻译：谷氨酸棒杆菌的质粒及其用途
公开(公告)号：US06777229B1
公开(公告)日：2004-08-17
申请号：US09704725
申请日：2000-11-03
申请人： Andreas Tauch , Jorn Kalinowski , Alfred Puhler , Georg Thierbach
发明人： Andreas Tauch , Jorn Kalinowski , Alfred Puhler , Georg Thierbach
IPC分类号： C12N1563
CPC分类号： C12N15/77 , C12P13/02 , C12P13/08
摘要： This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by 1.1 a length of ˜27.8 kbp and the restriction map shown in FIG. 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and 1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6, and plasmid pCRY4 is characterised by 1.4 a length of ˜48 kbp and the restriction map shown in FIG. 2 and 1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4 to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.
摘要翻译：本发明涉及从DSM号5616中沉积的谷氨酸棒杆菌菌株分离的相互相容的质粒pTET3和pCRY4，其中质粒pTET3的特征在于1.1，长度为约27.8kbp，图1所示的限制性图谱。 1,1.2复制区，其包含SEQ ID No： 1和1.3由SEQ ID No.3所示的赋予四环素抗性的tetA基因和赋予链霉素和壮观霉素抗性的aadA基因组成的抗生素抗性区域。 6，并且质粒pCRY4的特征在于1.4〜48kbp的长度以及图4所示的限制性图谱。 2和1.5复制区，其包含SEQ ID No：这些质粒能够在棒状细菌中自主复制的这些质粒的复合质粒载体和使用这些细菌生产L-氨基酸，维生素和核苷酸的方法。

10. 发明授权

US06171845B2 Mutant E. coli kiz strains for production of pantothenic acid 有权
标题翻译：用于生产泛酸的突变大肠杆菌kiz菌株
公开(公告)号：US06171845B2
公开(公告)日：2001-01-09
申请号：US09416756
申请日：1999-10-12
申请人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
发明人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
IPC分类号： C12N121
CPC分类号： C12N9/0008 , C12P7/42
摘要： The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
摘要翻译：本发明涉及通过扩增编码酮戊酸还原酶，特别是panE基因的核苷酸序列来单独地或彼此组合并任选地另外添加ilvC来制备和改进D-泛酸产生微生物的方法基因，含有这些核苷酸序列的微生物，以及D-泛酸的制备方法，其包括这些微生物的发酵，在培养基中或微生物细胞中浓缩泛酸，以及分离D-泛酸。

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