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1. 发明申请

US20130130267A1 REACTION VESSEL FOR PCR DEVICE AND METHOD OF PERFORMING PCR 有权
标题翻译：用于PCR装置的反应容器和执行PCR的方法
公开(公告)号：US20130130267A1
公开(公告)日：2013-05-23
申请号：US13698805
申请日：2011-05-19
申请人： Gerd Lüdke , Andreas Boos , Hassan Motejadded , Johannes Bacher
发明人： Gerd Lüdke , Andreas Boos , Hassan Motejadded , Johannes Bacher
IPC分类号： B01L7/00
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/0631 , B01L2200/10 , B01L2300/047 , B01L2300/0636 , B01L2300/0681 , B01L2300/087 , B01L2400/0487 , B01L2400/049
摘要： The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber (33) and after the PCR the reaction vessel (20) is configured to force the liquid sample via the spacer element (42) to the porous membrane (51) for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel (20) as well as a method for performing PCR are described.
摘要翻译：本发明提供一种用于PCR装置的反应容器（20）。反应容器（20）包括限定用于进行PCR的反应室（33）的样品瓶（32）和限定用于光学检测的储存室（63）的储存容器（62）。反应室（33）与液体供应口（34）流体连通，用于向反应室（33）供应含有至少一种目标DNA的液体样品。反应室（33）和储存室（63）通过间隔元件（42）和多孔膜（51）流体连通，用于将液体样品中的至少一个目标DNA杂交到特定的固定化杂交探针上。间隔元件（42）的下端延伸到反应室（33）中，但不到达其底部。间隔元件（42）的上端位于多孔膜（51）附近，多孔膜（51）由干燥状态和潮湿状态下具有不同物理性质的材料制成。在干燥状态下，多孔膜（51）允许空气以及液体通过。在湿状态下，多孔膜（51）仍然允许液体通过其中而不是空气通过，使得在PCR期间液体样品保留在反应室（33）中，并且在PCR之后，反应容器（20）为被配置为将液体样品经由间隔元件（42）强制到多孔膜（51），用于杂交和检测液体样品中的至少一种靶DNA。此外，描述包含这种反应容器（20）的PCR装置以及进行PCR的方法。

2. 发明申请

US20140242678A1 APPARATUS AND METHOD FOR A LYSIS OF A SAMPLE, IN PARTICULAR FOR AN AUTOMATED AND/OR CONTROLLED LYSIS OF A SAMPLE 有权
标题翻译：用于样品分析的装置和方法，特别是用于样品的自动和/或控制分析
公开(公告)号：US20140242678A1
公开(公告)日：2014-08-28
申请号：US14232391
申请日：2011-07-27
申请人： Andreas Boos , Gerd Lüdke , Johannes Bacher
发明人： Andreas Boos , Gerd Lüdke , Johannes Bacher
IPC分类号： C12M1/00 , C12N1/06
CPC分类号： C12M47/06 , C12N1/066 , G01N1/286 , G01N1/38 , G01N1/44 , G01N35/025 , G01N2035/00504 , G01N2035/00524 , G01N2035/0441
摘要： The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (31), at least one vial holder (90) which is configured to receive a vial (100), wherein the vial holder (90) is arranged on the disc (31), at least one driving device (20) which is configured to rotate the disc (31) and the vial holder (90), at least one heating device (60) which is configured to heat the sample to a determined incubation temperature, and—at least one control device (70) which is configured to control the driving device (20) and/or the heating device (60) by means of a timing and/or step control, and/or—at least one transmitting device (80) for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (60), and/or—wherein the driving device (20) is configured to rotate the disc (31) in a first direction (A1) and/or with a first speed, and to rotate the vial holder (90) in a second direction (A3) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.
摘要翻译：本发明提供了一种用于裂解程序的装置和方法，特别是用于样品，特别是生物样品的自动和/或受控裂解方法。该设备包括至少一个旋转圆盘（31），至少一个小瓶支架（90），其构造成接收小瓶（100），其中小瓶支架（90）布置在圆盘（31）上，至少一个驱动装置（20），其构造成使盘（31）和小瓶保持器（90）旋转;至少一个加热装置（60），其被配置为将样品加热至确定的培养温度;以及 - 至少一个控制设备（70），其被配置为通过定时和/或步进控制来控制驱动装置（20）和/或加热装置（60），和/或至少一个用于感应耦合的发送装置（80）用于能量和信号传输，其被配置为将用于加热的能量传输到加热装置（60），和/或 - 其中所述驱动装置（20）被配置为沿第一方向（A1）旋转所述盘（31）和/或以第一速度旋转，并且在第二方向（A3）和/或以第二速度旋转小瓶保持器（90）。该装置和方法适用于（自动）裂解程序，其中裂解可以以安全，有效和有效的方式进行。

3. 发明授权

US09963670B2 Apparatus and method for a lysis of a sample, in particular for an automated and/or controlled lysis of a sample 有权
公开(公告)号：US09963670B2
公开(公告)日：2018-05-08
申请号：US14232391
申请日：2011-07-27
申请人： Andreas Boos , Gerd Lüdke , Johannes Bacher
发明人： Andreas Boos , Gerd Lüdke , Johannes Bacher
IPC分类号： C12M1/00 , G01N1/28 , G01N1/38 , G01N1/44 , C12N1/06 , G01N35/02 , G01N35/04 , G01N35/00
CPC分类号： C12M47/06 , C12N1/066 , G01N1/286 , G01N1/38 , G01N1/44 , G01N35/025 , G01N2035/00504 , G01N2035/00524 , G01N2035/0441
摘要： The present invention provides an apparatus and a method for a lysis procedure, in particular for an automated and/or controlled lysis procedure of a sample, in particular a biological sample. The apparatus comprises at least one rotation disc (31), at least one vial holder (90) which is configured to receive a vial (100), wherein the vial holder (90) is arranged on the disc (31), at least one driving device (20) which is configured to rotate the disc (31) and the vial holder (90), at least one heating device (60) which is configured to heat the sample to a determined incubation temperature, and—at least one control device (70) which is configured to control the driving device (20) and/or the heating device (60) by means of a timing and/or step control, and/or—at least one transmitting device (80) for inductive coupling for energy and signal transmission, which is configured to transmit the energy for heating to the heating device (60), and/or—wherein the driving device (20) is configured to rotate the disc (31) in a first direction (A1) and/or with a first speed, and to rotate the vial holder (90) in a second direction (A3) and/or with a second speed. The apparatus and the method are adapted for an (automated) lysis procedure, wherein the lysis can be carried out in a safe, efficient and effective manner.

4. 发明授权

US09592511B2 Reaction vessel for PCR device and method of performing PCR 有权
标题翻译：用于PCR装置的反应容器和进行PCR的方法
公开(公告)号：US09592511B2
公开(公告)日：2017-03-14
申请号：US13698805
申请日：2011-05-19
申请人： Gerd Lüdke , Andreas Boos , Hassan Motejadded , Johannes Bacher
发明人： Gerd Lüdke , Andreas Boos , Hassan Motejadded , Johannes Bacher
IPC分类号： B01L7/00 , B01L3/00
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/0631 , B01L2200/10 , B01L2300/047 , B01L2300/0636 , B01L2300/0681 , B01L2300/087 , B01L2400/0487 , B01L2400/049
摘要： The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber (33) and after the PCR the reaction vessel (20) is configured to force the liquid sample via the spacer element (42) to the porous membrane (51) for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel (20) as well as a method for performing PCR are described.
摘要翻译：本发明提供一种用于PCR装置的反应容器（20）。反应容器（20）包括限定用于进行PCR的反应室（33）的样品瓶（32）和限定用于光学检测的储存室（63）的储存容器（62）。反应室（33）与液体供应口（34）流体连通，用于向反应室（33）供应含有至少一种目标DNA的液体样品。反应室（33）和储存室（63）通过间隔元件（42）和多孔膜（51）流体连通，用于将液体样品中的至少一个目标DNA杂交到特定的固定化杂交探针上。间隔元件（42）的下端延伸到反应室（33）中，但不到达其底部。间隔元件（42）的上端位于多孔膜（51）附近，多孔膜（51）由干燥状态和潮湿状态下具有不同物理性质的材料制成。在干燥状态下，多孔膜（51）允许空气以及液体通过。在湿状态下，多孔膜（51）仍然允许液体通过其中而不是空气通过，使得在PCR期间液体样品保留在反应室（33）中，并且在PCR之后，反应容器（20）为被配置为将液体样品经由间隔元件（42）强制到多孔膜（51），用于杂交和检测液体样品中的至少一种靶DNA。此外，描述包含这种反应容器（20）的PCR装置以及进行PCR的方法。

5. 外观设计

USD683044S1 Column adapter 有权
标题翻译：列适配器
公开(公告)号：USD683044S1
公开(公告)日：2013-05-21
申请号：US29409184
申请日：2011-12-21
申请人： Matthias Klein , Gerd Lüdke , Andreas Boos
设计人： Matthias Klein , Gerd Lüdke , Andreas Boos

6. 发明授权

US09598721B2 Universally applicable lysis buffer and processing methods for the lysis of bodily samples 有权
公开(公告)号：US09598721B2
公开(公告)日：2017-03-21
申请号：US13698216
申请日：2011-05-09
申请人： Matthias Klein , Gerd Lüdke , Andreas Boos
发明人： Matthias Klein , Gerd Lüdke , Andreas Boos
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1006
摘要： The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety of different sample types, such as different types of bodily samples relevant for the diagnosis of a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable.

7. 发明申请

US20130065223A1 Universally Applicable Lysis Buffer and Processing Methods for the Lysis of Bodily Samples 有权
标题翻译：普遍适用的裂解缓冲液和人体裂解液的加工方法
公开(公告)号：US20130065223A1
公开(公告)日：2013-03-14
申请号：US13698216
申请日：2011-05-09
申请人： Matthias Klein , Gerd Lüdke , Andreas Boos
发明人： Matthias Klein , Gerd Lüdke , Andreas Boos
IPC分类号： C12N1/06 , C12N1/00 , C12Q1/68 , C12N9/50 , C12N9/56
CPC分类号： C12Q1/6806 , C12N15/1006
摘要： The present invention provides a universally applicable lysis buffer comprising a chaotropic 5 agent, a reducing agent, and a proteolytic enzyme suitable for processing a wide variety o different sample types, such as different types of bodily samples relevant for the diagnosis o a respiratory disease. Furthermore, the present invention provides the use of a chaotropic agent, a reducing agent, and a proteolytic enzyme for the lysis of a broad spectrum of bodily samples. Moreover, the present invention provides a method for processing bodily samples which is universally applicable to the lysis of a variety of different types of bodily samples. Furthermore, the present invention provides methods for analyzing a bodily sample or for detecting the presence of a pathogen in a bodily sample, preferably, for diagnosing a respiratory disease, such as pneumonia or tuberculosis. Preferably, these methods are universally applicable to a variety of sample types, are applicable as one-tube-processes, are 15 suitable for performance in a high-throughput setting, and are automatable.
摘要翻译：本发明提供了一种普遍适用的裂解缓冲液，其包含离液序列5剂，还原剂和适用于处理各种不同样品类型的蛋白水解酶，例如与呼吸系统疾病诊断相关的不同类型的身体样品。此外，本发明提供了离液剂，还原剂和蛋白水解酶用于裂解广谱的身体样品的用途。此外，本发明提供了一种通常适用于各种不同类型的身体样品的裂解的身体样品的处理方法。此外，本发明提供了用于分析身体样品或用于检测身体样品中病原体的存在的方法，优选用于诊断呼吸系统疾病如肺炎或结核病。优选地，这些方法通常适用于各种样品类型，适用于单管工艺，适用于高通量设置中的性能，并且可自动化。

8. 发明授权

US09568424B2 Cartridge, system and method for automated medical diagnostics 有权
标题翻译：墨盒，系统和自动化诊断方法
公开(公告)号：US09568424B2
公开(公告)日：2017-02-14
申请号：US11917955
申请日：2006-06-16
申请人： Chris Van Haag , Michiel Johannes Jongerius , Danny Genius Aldegonda Schaefer , Adrianus Wilhelmus Dionisius Maria Van Den Bijgaart , Ronald De Gier , Michiel De Jong , Gerhard Pross , Johannes Bacher , Andreas Boos , Gerd Luedke , Jens-Peter Seher
发明人： Chris Van Haag , Michiel Johannes Jongerius , Danny Genius Aldegonda Schaefer , Adrianus Wilhelmus Dionisius Maria Van Den Bijgaart , Ronald De Gier , Michiel De Jong , Gerhard Pross , Johannes Bacher , Andreas Boos , Gerd Luedke , Jens-Peter Seher
IPC分类号： C12M1/36 , G01N21/64 , B01L3/00 , G01N35/00
CPC分类号： B01L7/52 , B01L3/502715 , B01L3/502738 , B01L3/545 , B01L2200/027 , B01L2200/028 , B01L2200/10 , B01L2300/02 , B01L2300/087 , B01L2300/1805 , G01N21/6428 , G01N35/00069 , G01N2035/00158
摘要： Cartridge and method for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a one or more nucleic acid sequences, and the cartridge includes a generic part and one or more separate application-specific parts, which are connectable to the generic part.
摘要翻译：用于检测包含一个或多个核酸序列的样品中靶核苷酸序列的存在，不存在和/或数量的盒和方法。盒包括一个或多个核酸序列，并且盒包括通用部分和一个或多个单独的应用特定部分，其可连接到通用部分。

9. 发明申请

US20080312105A1 Sensor For Biomolecules and a Method of Analysis Using Said Sensor 审中-公开
标题翻译：用于生物分子的传感器和使用所述传感器的分析方法
公开(公告)号：US20080312105A1
公开(公告)日：2008-12-18
申请号：US12158076
申请日：2006-12-15
申请人： Johannes Bacher , Andreas Boos , Gerd Luedke
发明人： Johannes Bacher , Andreas Boos , Gerd Luedke
IPC分类号： C40B50/18 , C40B60/12
CPC分类号： B01L3/502 , B01L2300/0636 , B01L2300/0681
摘要： Disclosed is a method of preparing a sensor for the analysis of a sample fluid, said sample fluid containing one or more target molecules. The method comprises the step of introducing said sample fluid into a chamber equipped with a porous substrate, one or more probe molecules being applied to said porous substrate and said probes being able to specifically bind to said one or more target molecules. The method further comprises the step of moving said substrate and said chamber relatively to each other in order to force said sample fluid through the pores of said porous substrate and to capture the one or more target molecules with the one or more probe molecules. Also disclosed is a sensor for the analysis of a sample fluid.
摘要翻译：公开了一种制备用于分析样品流体的传感器的方法，所述样品流体包含一种或多种靶分子。该方法包括将所述样品流体引入配备有多孔底物的室中的步骤，将一个或多个探针分子施加到所述多孔底物上，并且所述探针能够特异性结合所述一个或多个靶分子。该方法还包括相对于彼此移动所述衬底和所述室的步骤，以迫使所述样品流体通过所述多孔衬底的孔并用所述一个或多个探针分子俘获所述一个或多个靶分子。还公开了用于分析样品流体的传感器。

10. 发明申请

US20080300144A1 Sensor for Biomolecules and a Method for Preparing and Using the Same 审中-公开
标题翻译：生物分子传感器及其制备和使用方法
公开(公告)号：US20080300144A1
公开(公告)日：2008-12-04
申请号：US12158082
申请日：2006-12-13
申请人： Johannes Bacher , Andreas Boos , Gerd Luedke
发明人： Johannes Bacher , Andreas Boos , Gerd Luedke
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： G01N33/54366
摘要： Disclosed is a method of preparing a sensor for the analysis of a sample fluid, said sample fluid containing one or more target molecules. The method comprises the steps of applying a non-activated porous organic polymer membrane with probes in the form of an array of probe locations, said probes being able to specifically bind to said one or more target molecules. Furthermore, the method comprises the steps of blocking areas remaining free of probes of said porous organic polymer membrane with one or more blocking substances and forcing the sample fluid repeatedly in one or two directions through the pores of said porous organic polymer membrane. Also disclosed is a sensor for the analysis of a sample fluid.
摘要翻译：公开了一种制备用于分析样品流体的传感器的方法，所述样品流体包含一种或多种靶分子。该方法包括以探针位置阵列的形式施加非活化多孔有机聚合物膜的步骤，所述探针能够特异性地结合所述一个或多个靶分子。此外，该方法包括以下步骤：用一种或多种封闭物质阻挡剩余的不含所述多孔有机聚合物膜的探针的区域，并通过所述多孔有机聚合物膜的孔在一个或两个方向上反复强制样品流体。还公开了用于分析样品流体的传感器。

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