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    • 4. 发明授权
    • Method for assaying histone methylation enzyme activity
    • 测定组蛋白甲基化酶活性的方法
    • US09012166B2
    • 2015-04-21
    • US13639334
    • 2011-04-15
    • Norikazu NishinoYasushi TakemotoAkihiro ItoMinoru Yoshida
    • Norikazu NishinoYasushi TakemotoAkihiro ItoMinoru Yoshida
    • G01N21/78C12Q1/37C12Q1/48G01N33/68C07D311/18C07D311/82C07D473/32
    • C12Q1/37C07D311/18C07D311/82C07D473/32C12Q1/48G01N33/6875G01N2500/00Y02P20/55
    • Provided are a method for measuring histone methyltransferase activity, a method for screening for compounds that inhibit histone methyltransferase activity, a reagent kit for measuring histone methyltransferase activity, and a kit for screening for compounds that inhibit histone methyltransferase activity. A substrate compound represented by general formula (I): R1-X-K-R2 (I), or a salt thereof, wherein R1 represents a hydrogen atom or a protecting group for an amino terminus; X represents a peptide consisting of 0 or 1 or more amino acid residues; K represents a lysine residue; and R2 represents a dye label linked via an amide bond to the carbonyl terminus of a lysine residue, wherein the cleavage of the amide bond by peptidase changes the fluorescence property or chromogenic property of the dye label, and the methylation of the ε amino group of the lysine residue by the histone methyltransferase decreases susceptibility to peptidase, is used.
    • 提供了测定组蛋白甲基转移酶活性的方法,用于筛选抑制组蛋白甲基转移酶活性的化合物的方法,用于测定组蛋白甲基转移酶活性的试剂盒和用于筛选抑制组蛋白甲基转移酶活性的化合物的试剂盒。 由通式(I)表示的底物化合物:R1-X-K-R2(I)或其盐,其中R1表示氢原子或氨基末端的保护基; X表示由0或1个或更多个氨基酸残基组成的肽; K表示赖氨酸残基; R2表示通过酰胺键与赖氨酸残基的羰基末端连接的染料标记,其中通过肽酶切割酰胺键改变染料标记的荧光性质或显色性质,以及染料标记的甲基化。 使用组蛋白甲基转移酶的赖氨酸残基的氨基降低对肽酶的易感性。
    • 10. 发明申请
    • PREPREG AND METHOD OF MANUFACTURING THE PREPREG
    • PREPREG和制造PREPREG的方法
    • US20080248204A1
    • 2008-10-09
    • US12138729
    • 2008-06-13
    • Shirou AsadaHisao KobaAkihiro ItoKazutami MitaniKimihiro IkezakiKoki Wakabayashi
    • Shirou AsadaHisao KobaAkihiro ItoKazutami MitaniKimihiro IkezakiKoki Wakabayashi
    • B05D5/02
    • C08K9/08B29B15/10B29C37/0075C08J5/24
    • The present invention relates to a prepreg which is free from the phenomenon that air is trapped between layers to generate voids, has good handling characteristics and can produce molded articles having any complicated shape and also relates to a method of producing the prepreg. A reinforced-fiber sheet is impregnated with a matrix resin so as to form a continuous resin layer at least in the inside thereof, and then, a protective film having an irregular surface is applied to at least one surface of the reinforced-fiber sheet impregnated with the matrix resin such that the irregular surface faces the reinforced-fiber sheet. In this state, the prepreg is allowed to leave at 30 to 60° C. under the atmosphere for 12 hours or more. The resulting prepreg (1) is provided with a continuous resin layer (3a) which exists therein, wherein at least one surface of one side of the prepreg is constituted of a resin-impregnated part (3b) where the impregnated resin substantially exists and a fiber part (2a) where no resin substantially exists.
    • 本发明涉及一种预浸料坯,其没有空气被捕获在层之间以产生空隙的现象,具有良好的处理特性,并且可以生产具有任何复杂形状的模制品,并且还涉及制备预浸料的方法。 增强纤维片材用基质树脂浸渍,以便至少在其内部形成连续的树脂层,然后将具有不规则表面的保护膜施加到浸渍的增强纤维片材的至少一个表面上 基体树脂使得不规则表面面向增强纤维片材。 在这种状态下,允许预浸料在30〜60℃下在大气中离开12小时以上。 所得到的预浸料坯(1)上设置有存在于其中的连续树脂层(3a),其中预浸料坯一侧的至少一个表面由浸渍树脂基本存在的树脂浸渍部分(3b)构成 以及基本上不存在树脂的纤维部分(2a)。