专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5798262A Method for chromosomes classification 失效
公开(公告)号：US5798262A
公开(公告)日：1998-08-25
申请号：US844516
申请日：1997-04-18
申请人： Yuval Garini , Nir Katzir , David Wine , Dario Cabib
发明人： Yuval Garini , Nir Katzir , David Wine , Dario Cabib
IPC分类号： G01N33/48 , C12M1/00 , C12M3/00 , C12N15/09 , C12N15/11 , C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/453 , G01N1/28 , G01N21/64 , G01N21/75 , G01N33/483 , G01N33/50 , G01N33/52 , G01N33/569 , G01N33/58 , G01N37/00 , G02B21/00 , G02B21/16 , G06K9/00 , G06K9/76 , C12M1/34 , G06F15/00
CPC分类号： G01J3/12 , C12Q1/6816 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01N21/6428 , G01N21/6456 , G01N33/5005 , G01N33/56966 , G01N33/582 , G06K9/00127 , G06K9/0014 , G06K9/76 , G01J2003/2866 , G01J3/02 , G01N2021/6423 , G01N2021/6441
摘要： A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) using the pixels belonging to the other L-K chromosomes or portions of chromosomes for calculating the other L-K internal reference vectors, thereby finding all of the L internal reference vectors. A method for classification of L chromosomes or portions of chromosomes of a cell similarly painted using the above method for finding L internal reference vectors, and using the L reference vectors for classification of each of the pixels into one of L classification classes. And, images presenting color chromosomes.

2. 发明授权

US6055325A Color display of chromosomes or portions of chromosomes 失效
标题翻译：染色体或染色体部分的彩色显示
公开(公告)号：US6055325A
公开(公告)日：2000-04-25
申请号：US942327
申请日：1997-10-01
申请人： Yuval Garini , Dario Cabib , Robert A. Buckwald , Dirk G. Soenksen , Nir Katzir , David Wine , Moshe Lavi
发明人： Yuval Garini , Dario Cabib , Robert A. Buckwald , Dirk G. Soenksen , Nir Katzir , David Wine , Moshe Lavi
IPC分类号： G01B9/02 , A61B3/12 , A61B5/00 , C12Q1/68 , G01B11/06 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/453 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/50 , G01N33/569 , G01N33/58 , G06K9/00 , G06K9/76
CPC分类号： G01N21/6458 , A61B3/12 , C12Q1/6816 , C12Q1/6841 , C12Q1/6883 , G01B11/0675 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01J3/4535 , G01N21/6428 , G01N33/5005 , G01N33/56966 , G01N33/582 , G06K9/00127 , G06K9/76 , A61B3/1241 , C12Q2600/156 , G01J2003/2866 , G01J3/02 , G01N2021/6417 , G01N2021/6441
摘要： A color display comprising an image of all chromosomes or portions of chromosomes of a cell, each of the chromosomes or portions of chromosomes being painted with a different fluorophore or a combination of fluorophores, the image presenting the chromosomes or portions of chromosomes in different distinctive colors, wherein each of the chromosomes or portions of chromosomes is associated with one of the different distinctive colors.
摘要翻译：一种彩色显示器，其包括所有染色体或细胞染色体部分的图像，每个染色体或染色体部分用不同的荧光团或荧光团的组合进行染色，所述染色体或染色体的部分以不同的特征呈现其中每个染色体或染色体的部分与不同的独特颜色之一相关联。

3. 发明授权

US6043039A Method of and composite for in situ fluorescent hybridization 失效
标题翻译：原位荧光杂交法及复合方法
公开(公告)号：US6043039A
公开(公告)日：2000-03-28
申请号：US122661
申请日：1998-07-27
申请人： Irit Bar-Am , Cathy Janish , Yuval Garini , Dario Cabib , Robert A. Buckwald
发明人： Irit Bar-Am , Cathy Janish , Yuval Garini , Dario Cabib , Robert A. Buckwald
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/566
CPC分类号： C12Q1/6841
摘要： A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.
摘要翻译：一种荧光原位杂交方法，包括以下步骤：（a）获得物种的染色体扩散; （b）制备含有多个染色体涂料的杂化复合物，所述多个染色体涂料中的每一个用不同的荧光团或荧光团组合进行标记，使得所述杂交复合物中高度重复序列的平均比活性基本上等于杂交复合物中独特序列的平均比活性; （c）使杂交复合物变性并对杂交复合物进行条件以允许杂交复合物中的至少一部分高度重复序列进行再冷凝，同时杂交复合物中至少一部分独特序列保持单链; （d）在杂交条件下使杂交复合物与染色体扩散接触; （e）洗去杂交组合物的过量; 和（d）分析和呈现现在杂交的染色体扩增的图像。

4. 发明授权

US5784162A Spectral bio-imaging methods for biological research, medical diagnostics and therapy 失效
标题翻译：用于生物研究，医学诊断和治疗的光谱生物成像方法
公开(公告)号：US5784162A
公开(公告)日：1998-07-21
申请号：US571047
申请日：1995-12-12
申请人： Dario Cabib , Robert A. Buckwald , Zvi Malik , Yuval Garini , Nir Katzir , Dirk G. Soeknsen
发明人： Dario Cabib , Robert A. Buckwald , Zvi Malik , Yuval Garini , Nir Katzir , Dirk G. Soeknsen
IPC分类号： G01B11/00 , A61B3/00 , A61B3/12 , A61B5/00 , A61B10/00 , C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/443 , G01J3/45 , G01J3/453 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/483 , G01N33/50 , G01N33/569 , G01N33/58 , G02B21/36 , G06K9/00 , G06K9/76 , G01B9/02
CPC分类号： G01N21/6458 , C12Q1/6841 , C12Q1/6883 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01N21/6428 , G01N21/6486 , G01N33/5005 , G01N33/56966 , G01N33/582 , G06K9/00127 , G06K9/76 , G01J2003/2866 , G01J3/02 , G01N2021/6417 , G01N2021/6423
摘要： According to the present invention there are provided spectral imaging methods for biological research, medical diagnostics and therapy comprising the steps of (a) preparing a sample to be spectrally imaged; (b) viewing the sample through an optical device, the optical device being optically connected to an imaging spectrometer, the optical device and the imaging spectrometer obtaining a spectrum of each pixel of the sample by: (i) collecting incident light simultaneously from all pixels of the sample using collimating optics; (ii) passing the incident collimated light through an interferometer system having a number of elements, to form an exiting light beam; (iii) passing the exiting light beam through a focusing optical system which focuses the exiting light beam on a detector having a two-dimensional array of detector elements, so that at each instant each of the detector elements is the image of one pixel of the sample, so that the real image of the sample is stationary on the plane of the detector array, and so that each of the detector elements produces a signal which is a particular linear combination of light intensity emitted by the pixel at different wavelengths, wherein the linear combination is a function of the instantaneous optical path difference; (iv) rotating one or more of the elements of the interferometer system, so that the optical path difference between the two coherent beams generated by the interferometer system is scanned simultaneously for all the pixels of the sample; and (v) recording signals of each of the detector elements as function of time using a recording device to form a first spectral cube of data; and (c) interpreting the first spectral cube of data using a mathematical algorithm.
摘要翻译：根据本发明，提供了用于生物学研究，医学诊断和治疗的光谱成像方法，其包括以下步骤：（a）制备待成像的样品; （b）通过光学装置观察样品，光学装置光学连接到成像光谱仪，光学装置和成像光谱仪通过以下步骤获得样品的每个像素的光谱：（i）从所有像素同时收集入射光的样品使用准直光学; （ii）使入射的准直光通过具有多个元件的干涉仪系统，以形成出射的光束; （iii）使出射的光束通过聚焦光学系统，该聚焦光学系统将出射的光束聚焦在具有检测器元件的二维阵列的检测器上，使得在每个瞬间，每个检测器元件是样本，使得样本的真实图像在检测器阵列的平面上是静止的，并且使得每个检测器元件产生信号，其是由不同波长的像素发射的光强度的特定线性组合，其中线性组合是瞬时光程差的函数; （iv）旋转干涉仪系统的一个或多个元件，使得由干涉仪系统产生的两个相干光束之间的光程差同时扫描样品的所有像素; 以及（v）使用记录装置记录每个检测器元件的信号作为时间的函数，以形成数据的第一光谱立方体; 和（c）使用数学算法解释数据的第一个频谱立方体。

5. 发明申请

US20060250686A1 Optical microscope and method for obtaining an optical image 审中-公开
标题翻译：光学显微镜和获得光学图像的方法
公开(公告)号：US20060250686A1
公开(公告)日：2006-11-09
申请号：US11393523
申请日：2006-03-29
申请人： Yuval Garini , Ian Young
发明人： Yuval Garini , Ian Young
IPC分类号： G02B21/00
CPC分类号： G01Q60/22 , G02B21/0032 , G02B21/06
摘要： The invention relates to an optical microscope, comprising, at least a light source, a carrier for an object to be examined, a detector for registering the illuminated object, and a light path that during operation runs substantially from the light source to the object and form the object to the detector, wherein a metallic film having a periodic hole array is placed in the light path between the light source and the object, and wherein the carrier of the object is provided with a drive to allow the same to be adjusted in the plane of the carrier, wherein the holes of the metallic thin film have a diameter that is smaller than approximately 250 nm, in that the drive is designed for adjusting the carrier for the object in an orientation perpendicular to the plane of the carrier, and in that a processing device is provided that is connected with the detector for constructing a three-dimensional image of the object.
摘要翻译：本发明涉及一种光学显微镜，包括至少一个光源，用于待检测物体的载体，用于登记照射物体的检测器和在操作期间基本上从光源到物体运行的光路，以及形成物体到检测器，其中具有周期性孔阵列的金属膜被放置在光源和物体之间的光路中，并且其中物体的载体设置有驱动器，以允许其被调节载体的平面，其中金属薄膜的孔具有小于约250nm的直径，因为驱动器设计成用于在垂直于载体的平面的方向上调整用于物体的载体，以及因为提供了与用于构建物体的三维图像的检测器连接的处理装置。

6. 发明授权

US5871932A Method of and composite for fluorescent in situ hybridization 失效
标题翻译：原位荧光杂交法及复合方法
公开(公告)号：US5871932A
公开(公告)日：1999-02-16
申请号：US25131
申请日：1998-02-17
申请人： Irit Bar-Am , Yuval Garini , Dario Cabib
发明人： Irit Bar-Am , Yuval Garini , Dario Cabib
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6841
摘要： A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorohores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (d) analyzing and presenting images of the now hybridized chromosome spread.
摘要翻译：一种荧光原位杂交方法，包括以下步骤：（a）获得物种的染色体扩散; （b）制备含有多个染色体涂料的杂化复合物，所述多个染色体涂料中的每一个都用不同的荧光团或组合的荧光体标记，使得杂交复合物中高度重复序列的平均比活性基本上等于杂交复合物中独特序列的平均比活性; （c）使杂交复合物变性并对杂交复合物进行条件以允许杂交复合物中的至少一部分高度重复序列进行再冷凝，同时杂交复合物中至少一部分独特序列保持单链; （d）在杂交条件下使杂交复合物与染色体扩散接触; （e）洗去杂交组合物的过量; 和（d）分析和呈现现在杂交的染色体扩增的图像。

7. 发明授权

US5834203A Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore 失效
标题翻译：因此，使用多个宽带滤波器和硬件根据其频谱将像素分类为组的方法
公开(公告)号：US5834203A
公开(公告)日：1998-11-10
申请号：US917213
申请日：1997-08-25
申请人： Nir Katzir , David Wine , Yuval Garini , Dario Cabib
发明人： Nir Katzir , David Wine , Yuval Garini , Dario Cabib
IPC分类号： G01N21/64 , G01N15/14 , G01N21/78 , G01N33/533 , G01N33/566 , G01N33/58 , G02B21/06 , C12Q1/68 , F21V9/08 , G02B21/16
CPC分类号： G01N21/6428 , G01N21/6458 , G02B21/06 , G01N15/1475 , G01N2021/6441
摘要： A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.
摘要翻译：根据其与单个荧光团或选自多个荧光团的荧光团的组合的关联或每个荧光团具有表征激发和发射光谱以及指定激发和发射峰值的方式，将像素分类为像素组的方法，所述方法包括：（a）提供多对宽带激励滤波器和宽带发射滤波器的步骤; （b）每个像素的激发荧光团，其中通过一个宽带激发滤光器之一滤光，并且记录经过其成对发射滤光片之后检索的发射光强度; （c）对于所有多对滤波器重复步骤（b），使得每个像素可以由多个维度的向量表示，维数等于多对滤波器的数量过滤器（c）使用用于评估每个像素中的每个多个荧光团的存在的算法，从而根据其与单个荧光团或荧光团的组合的关联将每个像素分类为一组像素。

8. 发明授权

US08849579B2 Methods of detecting and monitoring cancer using 3D analysis of centromeres 有权
标题翻译：使用着丝粒的3D分析检测和监测癌症的方法
公开(公告)号：US08849579B2
公开(公告)日：2014-09-30
申请号：US12443781
申请日：2007-09-28
申请人： Sabine Mai , Yuval Garini , Rahul Sarkar , Bartholomeus J. Vermolen
发明人： Sabine Mai , Yuval Garini , Rahul Sarkar , Bartholomeus J. Vermolen
IPC分类号： G06F19/00 , G01N33/574 , C12Q1/68 , G06K9/00 , G01N33/50 , G01N15/14
CPC分类号： G06F19/345 , C12Q1/6886 , G01N15/1475 , G01N33/5035 , G01N33/5091 , G01N33/574 , G01N33/57426 , G06K9/0014 , G16H50/20
摘要： The present application relates to a method of detecting and monitoring cancer or precancer in a cell using three-dimensional analysis to assess centromere organization. In addition, the application relates to a method and system for characterizing the 3D organization of centromeres.
摘要翻译：本申请涉及使用三维分析来检测和监测细胞中的癌症或癌前期的方法以评估着丝粒组织。此外，该申请涉及用于表征着丝粒的3D组织的方法和系统。

9. 发明授权

US6165734A In-situ method of analyzing cells 有权
标题翻译：分析细胞的原位方法
公开(公告)号：US6165734A
公开(公告)日：2000-12-26
申请号：US196690
申请日：1998-11-20
申请人： Yuval Garini , George McNamara , Dirk Soenksen , Dario Cabib , Robert A Buckwald
发明人： Yuval Garini , George McNamara , Dirk Soenksen , Dario Cabib , Robert A Buckwald
IPC分类号： C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/453 , G01N1/30 , G01N21/64 , G01N21/78 , G01N33/50 , G01N33/53 , G01N33/533 , G01N33/535 , G01N33/569 , G01N33/58 , G06K9/00 , G06K9/76 , G01N33/567 , C12Q1/42 , C12Q1/54
CPC分类号： C12Q1/6841 , C12Q1/6883 , G01J3/02 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01J2003/2866 , G01N1/30 , G01N21/6428 , G01N21/6456 , G01N33/5005 , G01N33/53 , G01N33/56966 , G01N33/582 , G01N2021/6423 , G01N2021/6441 , G06K9/00127 , G06K9/76 , Y10S435/968
摘要： A method of in situ analysis of a biological sample comprising the steps of (a) staining the biological sample with N stains of which a first stain is selected from the group consisting of a first immunohistochemical stain, a first histological stain and a first DNA ploidy stain, and a second stain is selected from the group consisting of a second immunohistochemical stain, a second histological stain and a second DNA ploidy stain, with provisions that N is an integer greater than three and further that (i) if the first stain is the first immunohistochemical stain then the second stain is either the second histological stain or the second DNA ploidy stain; (ii) if the first stain is the first histological stain then the second stain is either the second immunohistochemical stain or the second DNA ploidy stain; whereas (iii) if the first stain is the first DNA ploidy stain then the second stain is either the second immunohistochemical stain or the second histological stain; and (b) using a spectral data collection device for collecting spectral data from the biological sample, the spectral data collection device and the N stains are selected such that a spectral component associated with each of the N stains is collectable.
摘要翻译：一种生物样品的原位分析方法，包括以下步骤：（a）染色具有N染色的生物样品，其中第一染色选自第一免疫组织化学染色，第一组织染色和第一DNA倍体染色，第二染色选自第二免疫组织化学染色，第二组织学染色和第二DNA倍性染色，其规定N为大于3的整数，并且（i）如果第一染色是第一次免疫组织化学染色，第二次染色是第二次组织染色或第二次DNA倍体染色; （ii）如果第一染色是第一组织染色，则第二染色是第二免疫组织化学染色或第二DNA倍体染色; 而（iii）如果第一个染色是第一个DNA倍性染色，则第二次染色是第二次免疫组化染色或第二次组织染色; 和（b）使用用于收集来自生物样品的光谱数据的光谱数据采集装置，选择光谱数据采集装置和N染色，使得与每个N染色体相关联的光谱分量是可收集的。

10. 发明授权

US6066459A Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding 失效
标题翻译：同时检测原位杂交和多色染色体绘制和条带的多种荧光团的方法
公开(公告)号：US6066459A
公开(公告)日：2000-05-23
申请号：US100104
申请日：1998-06-19
申请人： Yuval Garini , Dario Cabib , Robert A. Buckwald , Thomas Ried , Dirk G. Soenksen
发明人： Yuval Garini , Dario Cabib , Robert A. Buckwald , Thomas Ried , Dirk G. Soenksen
IPC分类号： C12N15/09 , C12Q1/68 , G01J3/02 , G01J3/12 , G01J3/26 , G01J3/28 , G01J3/44 , G01J3/453 , G01N21/64 , G01N33/50 , G01N33/569 , G01N33/58 , G02B21/16 , G06K9/00 , G06K9/76 , C07H21/04
CPC分类号： G01N21/6452 , C12Q1/6841 , C12Q1/6883 , G01J3/12 , G01J3/1256 , G01J3/26 , G01J3/2823 , G01J3/4406 , G01J3/453 , G01N21/6428 , G01N21/6458 , G01N33/5005 , G01N33/56966 , G01N33/582 , G06K9/00127 , G06K9/76 , C12Q2600/156 , G01J2003/2866 , G01J3/02 , G01N2021/6417 , G01N2021/6441
摘要： A spectral imaging method for simultaneous detection of multiple fluorophores aimed at detecting and analyzing fluorescent in situ hybridizations employing numerous chromosome paints and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores for color karyotyping, and at multicolor chromosome banding, wherein each chromosome acquires a specifying banding pattern, which pattern is established using groups of chromosome fragments labeled with various fluorophore or combinations of fluorophores.
摘要翻译：用于同时检测多个荧光团的光谱成像方法，其目的是检测和分析使用多种染色体涂料和/或基因座特异性探针的荧光原位杂交，每个染色体涂料和/或基因座特异性探针用不同的荧光团或用于颜色核型分析的荧光团的组合以及多色染色体条带标记，其中每个染色体获得指定的条带图案，使用由各种荧光团标记的染色体片段或荧光团的组合来建立该图案。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式