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    • 2. 发明申请
    • METHOD AND DEVICE FOR LOW FREQUENCY VIBRATION EXCITATION USING ULTRASONIC WAVE
    • 使用超声波的低频振动激励的方法和装置
    • US20110314916A1
    • 2011-12-29
    • US12825961
    • 2010-06-29
    • Jin-Ho ParkDoo-Byung YoonYoung-Chul ChoiSung Hwan Shin
    • Jin-Ho ParkDoo-Byung YoonYoung-Chul ChoiSung Hwan Shin
    • G01N29/12
    • G01N29/348G01N29/42G01N2291/106
    • Provided is a method and device for low frequency vibration excitation, which may generate a low frequency using a plurality of ultrasonic generators for high frequency. The method generates ultrasonic waves using a plurality of ultrasonic generators attached to a target structure to induce a beat phenomenon, and extract a frequency lower than a frequency of each of the plurality of the ultrasonic generators to measure a property of the target structure, and thereby may be freely applied to a target structure, regardless of a shape of the target structure such as a plate, a curved pipe, and the like, using a relatively small-sized ultrasonic sensor for high frequency, and may excite a specific frequency of an acceleration range, so that the ultrasonic excitation method may be applicable in a relatively poor Signal-to-Noise Ratio (SNR) range.
    • 提供了一种用于低频振动激励的方法和装置,其可以使用多个用于高频的超声波发生器来产生低频。 该方法使用附着到目标结构的多个超声波发生器产生超声波,以引起拍子现象,并且提取比多个超声波发生器的频率低的频率来测量目标结构的特性,从而 可以使用相对较小尺寸的用于高频率的超声波传感器,而不管诸如板,弯曲管等的目标结构的形状如何,可以自由地施加到目标结构,并且可以激发特定的频率 加速度范围,使得超声波激发方法可适用于相对较差的信噪比(SNR)范围。
    • 5. 发明申请
    • METHOD OF PREPARING siRNAs FOR SELECTIVE INHIBITION OF TARGET mRNA ISOTYPES
    • 制备用于选择性抑制靶标mRNA同功酶的siRNA的方法
    • US20090325291A1
    • 2009-12-31
    • US12304707
    • 2007-06-12
    • Seong Min ParkYoung Joo KimYoung Chul ChoiHan Oh ParkSo Rim Choung
    • Seong Min ParkYoung Joo KimYoung Chul ChoiHan Oh ParkSo Rim Choung
    • C12N5/02G01N33/48C07H21/02
    • C12N15/111C12N2320/10
    • A method of preparing siRNAs for selective inhibition of target mRNA isotypes comprises: dividing target mRNA isotypes intended to inhibit the expression thereof and non-target mRNA isotypes from the mRNA isotypes of a gene; allotting a common location information region (A) of exons on genome DNA corresponding to the target mRNA isotypes; allotting a location information region (B) present specifically in exons of genome DNA corresponding to target mRNAs by excluding the location information region of exons on genome DNA corresponding to non-target mRNA from the location information region (A); determining base sequences in the target mRNAs corresponding to the location information region (B); and obtaining siRNA sequences for inhibiting the determined base sequences specifically. The method of the present invention can be used to prepare siRNAs for selective inhibition of specific target mRNA isotypes in a gene having several isotypes by alternative splicing, and enables siRNA design for all the genes in genome, making good tool for functional genomics study.
    • 制备用于选择性抑制靶mRNA同种型的siRNA的方法包括:从基因的mRNA同种型分离旨在抑制其表达的靶mRNA同种型和非靶mRNA同种型; 在对应于靶mRNA同种型的基因组DNA上分配外显子的共同位置信息区(A); 通过从所述位置信息区域(A)中排除与非靶mRNA相对应的基因组DNA上的外显子的位置信息区,分配特定存在于与靶mRNA相对应的基因组DNA外显子中的位置信息区(B)。 确定对应于位置信息区域(B)的目标mRNA中的碱基序列; 并获得特异性抑制所确定的碱基序列的siRNA序列。 本发明的方法可用于制备用于通过选择性剪接在具有多个同种型的基因中特异性靶mRNA同种型的选择性抑制的siRNA,并且使得能够对基因组中所有基因进行siRNA设计,为功能基因组学研究提供了良好的工具。