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    • 1. 发明授权
    • Folding trailer
    • 折叠拖车
    • US08360462B2
    • 2013-01-29
    • US12572253
    • 2009-10-01
    • William Rodgers Mayfield
    • William Rodgers Mayfield
    • B60D1/54
    • B62D63/061
    • A foldable trailer is disclosed having the folding means provided by a winch that operates in conjunction with a lifting member enabling the trailer to be winched into an upright position for storage. The load bearing surface can be made of one section or two sections that fold together to allow for more compact storage. Through the lifting member the winch provides upward motion of the front of the load bearing surface while simultaneously causing the hingeably attached tongue to fold downward; continued winching will result in a vertically folded and storable position. The invention may be applied to a tilting trailer.
    • 公开了一种可折叠拖车,其具有由绞盘提供的折叠装置,该绞盘与提升构件一起操作,使得拖车能够被绞盘到用于存储的直立位置。 承载面可以由折叠在一起的一个或两个部分制成,以便更紧凑的储存。 通过提升构件,绞盘提供承载表面前部的向上运动,同时使可铰接的舌头向下折叠; 连续绞车将导致垂直折叠和可储存的位置。 本发明可以应用于倾斜拖车。
    • 5. 发明申请
    • Targeted fusion proteins and methods for the characterization of cellular membrane domains
    • 靶向融合蛋白和表征细胞膜结构域的方法
    • US20060281133A1
    • 2006-12-14
    • US11414158
    • 2006-04-27
    • William Rodgers
    • William Rodgers
    • G01N33/53C07H21/04C12P21/04C07K14/435
    • C12N9/1205C07K14/705C07K2319/00C07K2319/02C07K2319/033C07K2319/60G01N33/5041G01N33/92
    • Cell membranes containing glycolipid-enriched membrane (GEM) and non-glycolipid-enriched membrane (non-GEM) domains are targeted using fusion proteins that are anchored in the cell membrane. Fusion proteins to target GEM (or non-GEM) domains are comprised of a selected fluorescent polypeptide, a membrane-targeting sequence of p56Lck (or pp60c-Src for non-GEM domains) and a linker inserted between the polypeptide and the membrane targeting sequence. Localization of fusion proteins in GEM and non-GEM domains is assessed using techniques including confocal microscopy, fluorescence-based techniques, and membrane fractionation. Using these techniques, compounds are screened for their effect on GEM and non-GEM domains of live cells. These fusion proteins therefore represent useful tools for studying subcellular trafficking and the function of discrete compartments in the plasma membrane.
    • 含有富含脂肪糖的膜(GEM)和非糖脂富集膜(非GEM)结构域的细胞膜使用锚定在细胞膜中的融合蛋白进行靶向。 用于靶向GEM(或非GEM)结构域的融合蛋白由选择的荧光多肽组成,非选择性荧光多肽,非靶细胞的靶向p56的序列(或pp60S-Src / -GEM结构域)和插入多肽和膜靶向序列之间的接头。 使用包括共焦显微镜,基于荧光的技术和膜分级技术的技术来评估GEM和非GEM结构域中融合蛋白的定位。 使用这些技术,筛选化合物对活细胞的GEM和非GEM结构域的影响。 因此,这些融合蛋白是用于研究亚细胞运输和质膜中离散隔室功能的有用工具。