会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 2. 发明申请
    • Nucleic acid molecules encoding hyperactive nucleoside di-phosphate kinase 2 and uses thereof
    • US20070136897A1
    • 2007-06-14
    • US11247236
    • 2005-10-12
    • Jeong-II KimYun-Jeong HanSeong-Hee KimPill-Soon Song
    • Jeong-II KimYun-Jeong HanSeong-Hee KimPill-Soon Song
    • A01H1/00C07H21/04C12N5/04C12N15/82
    • C12N9/1229C12N15/8261C12N15/8271Y02A40/146
    • The present invention includes modified Arabidopsis Nucleoside Di-Phosphate Kinase 2 (NDPK2) nucleic acid molecules whose enzymatic activity have been increased (i.e. hyperactive). NDPKs are ubiquitous housekeeping enzymes that catalyze the transfer of γ-phosphoryl group from a nucleoside triphosphate (NTP) to a nucleoside diphosphate (NDP), and also multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. In plants, NDPKs are reported to play a key role in the signaling of both stress and light. Among three NDPKs (NDPK1, NDPK2, NDPK3) in a model plant, Arabidopsis thaliana, NDPK2 was reported as a positive signal transducer of phytochrome-mediated plant light signaling and to regulate cellular redox state, which enhances multiple stress tolerance in transgenic plants. Thus, the plants with the hyperactive NDPK2 are expected to possess higher efficiency of light utilization and enhanced tolerance to various environmental stresses such as cold, salt, and oxidative stresses. Since abiotic stress is one of the most important factors to limit the productivity of many crops, the hyperactive NDPK2 can be used for the development of high-yielding multiple stress tolerant plants with higher efficiency of light utilization. In this invention, several hyperactive NDPK2 were generated by C-terminal deletion and site-directed mutagenesis. Therefore, the present invention can be utilized to develop multiple stress tolerant and efficiently light-utilizing plants, which can eventually increase crop yields. The invention also includes plants having at least one cell expressing the modified NDPK2, vectors comprising at least one portion of the modified NDPK2 nucleic acids, and methods using such vectors for producing plants with enhanced light sensitivity and stress tolerance.
    • 3. 发明授权
    • Nucleic acid molecules encoding hyperactive mutant phytochromes and uses thereof
    • 编码活性突变体植物色素的核酸分子及其用途
    • US06916973B2
    • 2005-07-12
    • US10134500
    • 2002-04-30
    • Jeong Il KimPill Soon SongKi Young YangMoon Soo SohChung Mo Park
    • Jeong Il KimPill Soon SongKi Young YangMoon Soo SohChung Mo Park
    • C12N15/29C12N15/82C12N15/87A01H1/00
    • C12N15/827C12N15/8261Y02A40/146
    • The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which DNA sequences coding for “active site” amino acid residues have been mutated to generate hyperactive phytochromes. In particular; a serine/threonine residue at the hinge between the N- and C-terminal domains as well as at the N-terminal serine/threonine cluster of phytochromes (e.g., serine-598 and serine-7 in oat phytochrome A) for (a) Pr/Pfr-dependent phosphorylation and (b) dephosphorylation by a phytochrome phosphatase (PP2A) was substituted with alanine. (c) In addition, amino acid residues within the phytochrome chromophore pocket are mutated to generate the bathchromic shift of the Pr-absorption band of both wild type and above-mentioned mutant phytochromes. The plants with the bathchromically shifted absorption spectrum are expected to respond to the canopy and shade conditions for growth and greening responses to far-red light with greater efficiency than are the wild type plants with normal absorption band maxima. These mutative modifications confer hyperactivity to the far-red light responsive phytochromes A. Thus, the biological activity of the modified oat PHYA was shown to be hyperactive compared to wild type PHYA, characterized by its ability to reduce internode elongation of adult plants. Overexpression of the phytochrome phosphatase exhibits a suppressed growth with shorter internodes and belated flowering, qualitatively consistent with the phenotype of a ser598ala mutant oat phytochrome. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with reduced stature.
    • 本发明包括修饰的植物色素A(PHYA)核酸分子,其中编码“活性位点”氨基酸残基的DNA序列已被突变以产生多活性植物色素。 尤其是; 在N-和C-末端结构域之间以及在植物色素的N-末端丝氨酸/苏氨酸簇(例如,燕麦植物色素A中的丝氨酸-598和丝氨酸-7)上的铰链处的丝氨酸/苏氨酸残基用于(a) Pr / Pfr依赖性磷酸化和(b)植物色素磷酸酶(PP2A)的去磷酸化被丙氨酸取代。 (c)此外,植物色素发色团口袋内的氨基酸残基被突变以产生野生型和上述突变体植物色素的Pr-吸收带的色变。 与具有正常吸收带最大值的野生型植物相比,具有浴变色吸收光谱的植物预期响应于冠层和阴凉条件对远红光的生长和绿化反应的效率更高。 这些突变性修饰赋予远红色光响应植物色素A的多动作用。因此,与野生型PHYA相比,修饰的燕麦PHYA的生物学活性显示为多活性,其特征在于其减少成年植物的节间伸长的能力。 植物色素磷酸酶的过表达显示抑制生长,具有较短的节间和迟缓的开花,与ser598ala突变体燕麦植物色素的表型定性一致。 本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物,包含至少一部分修饰的PHYA核酸的载体,以及使用这种载体产生具有降低身材的植物的方法。
    • 4. 发明授权
    • Container and/or closure therefor
    • 集装箱和/或关闭
    • US5346083A
    • 1994-09-13
    • US173280
    • 1993-12-27
    • Pill-Soon SongWilliam R. Parker, Jr.
    • Pill-Soon SongWilliam R. Parker, Jr.
    • B65D1/02B65D39/08B65D41/04B65D45/06B65D53/00
    • B65D41/0414B65D1/023B65D39/08B65D45/06
    • To permit a container and a cap to remate with each other during resealing without fouling by solid debris such as small particles without exceptional preparations to withstand the deteriorating effects of corrosive materials and solvents and to consistently and quickly form a gas-tight seal, the container has a flat angled surface and the cap has cooperating edge that engages the flat surface in a closed line when the cap is fully inserted with a solid closed surface within the line, the closed line having a width perpendicular to its length within a range of 0.5 millimeters to 1.5 millimeters. The cap is closed by screw threads or the like that force the cooperating surfaces together with a pressure of at least 100 psi at an angle of between 75 degrees and 105 degrees.
    • 为了允许容器和盖子在重新密封期间彼此重新凝结,而不会由诸如小颗粒的固体碎屑污染而没有特殊的制剂以抵抗腐蚀性材料和溶剂的劣化作用并且始终如一地形成气密密封,容器 具有平坦的倾斜表面,并且当盖完全插入线内的实心封闭表面时,盖具有与封闭线中的平坦表面接合的配合边缘,该闭合线具有垂直于其长度的宽度在0.5的范围内 毫米至1.5毫米。 帽被螺纹或类似物封闭,螺纹等将压配合表面以至少100psi的压力以75度和105度之间的角度相加。
    • 8. 发明授权
    • Method of fabricating semiconductor devices having buried contact plugs
    • 制造具有埋入式接触塞的半导体器件的方法
    • US07749834B2
    • 2010-07-06
    • US11364635
    • 2006-02-27
    • Je-Min ParkYoo-Sang HwangSeok-Soon Song
    • Je-Min ParkYoo-Sang HwangSeok-Soon Song
    • H01L21/8242
    • H01L27/10855H01L27/10817
    • A method includes forming a lower dielectric layer on a semiconductor substrate, forming a bit line landing pad and a storage landing pad that penetrate the lower dielectric layer, covering the lower dielectric layer, the bit line landing pad, and the storage landing pad with an intermediate dielectric layer, forming an upper dielectric layer on the intermediate dielectric layer, partially removing the upper dielectric layer and the intermediate dielectric layer to form a contact opening that exposes the storage landing pad and a portion of the lower dielectric layer, forming a contact spacer on an inner wall of the contact opening, and filling the contact opening with a contact plug, a top surface of the contact plug larger than a surface of the contact plug that is in contact with the storage landing pad, the top surface of the contact plug eccentric in relation to the storage landing pad.
    • 一种方法包括在半导体衬底上形成下电介质层,形成位线着陆焊盘和穿透下电介质层的存储着陆焊盘,覆盖下电介质层,位线着陆焊盘和存储着陆焊盘 中间介电层,在中间介电层上形成上电介质层,部分地去除上电介质层和中间电介质层,以形成暴露存储着陆焊盘和下电介质层的一部分的接触开口,形成接触间隔物 在接触开口的内壁上,并用接触塞填充接触开口,接触插塞的顶表面大于接触插塞的与储存着陆垫接触的表面,触头顶表面 相对于存储着陆垫插头偏心。