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1. 发明授权

US06916973B2 Nucleic acid molecules encoding hyperactive mutant phytochromes and uses thereof 失效
标题翻译：编码活性突变体植物色素的核酸分子及其用途
公开(公告)号：US06916973B2
公开(公告)日：2005-07-12
申请号：US10134500
申请日：2002-04-30
申请人： Jeong Il Kim , Pill Soon Song , Ki Young Yang , Moon Soo Soh , Chung Mo Park
发明人： Jeong Il Kim , Pill Soon Song , Ki Young Yang , Moon Soo Soh , Chung Mo Park
IPC分类号： C12N15/29 , C12N15/82 , C12N15/87 , A01H1/00
CPC分类号： C12N15/827 , C12N15/8261 , Y02A40/146
摘要： The present invention includes modified phytochrome A (PHYA) nucleic acid molecules in which DNA sequences coding for “active site” amino acid residues have been mutated to generate hyperactive phytochromes. In particular; a serine/threonine residue at the hinge between the N- and C-terminal domains as well as at the N-terminal serine/threonine cluster of phytochromes (e.g., serine-598 and serine-7 in oat phytochrome A) for (a) Pr/Pfr-dependent phosphorylation and (b) dephosphorylation by a phytochrome phosphatase (PP2A) was substituted with alanine. (c) In addition, amino acid residues within the phytochrome chromophore pocket are mutated to generate the bathchromic shift of the Pr-absorption band of both wild type and above-mentioned mutant phytochromes. The plants with the bathchromically shifted absorption spectrum are expected to respond to the canopy and shade conditions for growth and greening responses to far-red light with greater efficiency than are the wild type plants with normal absorption band maxima. These mutative modifications confer hyperactivity to the far-red light responsive phytochromes A. Thus, the biological activity of the modified oat PHYA was shown to be hyperactive compared to wild type PHYA, characterized by its ability to reduce internode elongation of adult plants. Overexpression of the phytochrome phosphatase exhibits a suppressed growth with shorter internodes and belated flowering, qualitatively consistent with the phenotype of a ser598ala mutant oat phytochrome. The invention also includes plants having at least one cell expressing the modified PHYA, vectors comprising at least one portion of the modified PHYA nucleic acids, and methods using such vectors for producing plants with reduced stature.
摘要翻译：本发明包括修饰的植物色素A（PHYA）核酸分子，其中编码“活性位点”氨基酸残基的DNA序列已被突变以产生多活性植物色素。尤其是; 在N-和C-末端结构域之间以及在植物色素的N-末端丝氨酸/苏氨酸簇（例如，燕麦植物色素A中的丝氨酸-598和丝氨酸-7）上的铰链处的丝氨酸/苏氨酸残基用于（a） Pr / Pfr依赖性磷酸化和（b）植物色素磷酸酶（PP2A）的去磷酸化被丙氨酸取代。（c）此外，植物色素发色团口袋内的氨基酸残基被突变以产生野生型和上述突变体植物色素的Pr-吸收带的色变。与具有正常吸收带最大值的野生型植物相比，具有浴变色吸收光谱的植物预期响应于冠层和阴凉条件对远红光的生长和绿化反应的效率更高。这些突变性修饰赋予远红色光响应植物色素A的多动作用。因此，与野生型PHYA相比，修饰的燕麦PHYA的生物学活性显示为多活性，其特征在于其减少成年植物的节间伸长的能力。植物色素磷酸酶的过表达显示抑制生长，具有较短的节间和迟缓的开花，与ser598ala突变体燕麦植物色素的表型定性一致。本发明还包括具有至少一个表达经修饰的PHYA的细胞的植物，包含至少一部分修饰的PHYA核酸的载体，以及使用这种载体产生具有降低身材的植物的方法。

2. 发明申请

US20120226739A1 SERVER, SYSTEM, AND METHOD FOR OFFERING DISTRIBUTED SERVICE 审中-公开
标题翻译：服务器，系统和分发服务提供方法
公开(公告)号：US20120226739A1
公开(公告)日：2012-09-06
申请号：US13226828
申请日：2011-09-07
申请人： Ki-yong KIM , Chun-un KANG , Ki-young YANG
发明人： Ki-yong KIM , Chun-un KANG , Ki-young YANG
IPC分类号： G06F15/16
CPC分类号： H04W4/50 , H04L67/2809
摘要： a server, system and method for offering a distributed service are provided. The service distribution server includes: a first communication unit which communicates with a client; a second communication unit which communicates with a plurality of external devices which respectively provide a plurality of services; and a first controller which selects one among the plurality of services based on information about the client and a requested service if receiving a service request from the client, and controls the first communication unit and the second communication unit to cause an external device providing the selected service among the plurality of external devices implement the requested service.
摘要翻译：提供了一种用于提供分布式服务的服务器，系统和方法。服务分发服务器包括：与客户端通信的第一通信单元; 第二通信单元，其与分别提供多个服务的多个外部设备进行通信; 以及第一控制器，其基于关于所述客户端的信息和所请求的服务，如果从所述客户端接收到服务请求，则选择所述多个服务中的一个，并且控制所述第一通信单元和所述第二通信单元以使外部设备提供所选择的多个外部设备之间的服务实现所请求的服务。

3. 发明授权

US07164013B2 Hyperactive light signal related molecule of HFR1-ΔN105 and transgenic plant thereof 失效
标题翻译： HFR1-DeltaN105的多活性光信号相关分子及其转基因植物
公开(公告)号：US07164013B2
公开(公告)日：2007-01-16
申请号：US10641100
申请日：2003-08-15
申请人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
发明人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
IPC分类号： C12N15/29 , C12N15/52
CPC分类号： C12N15/8237
摘要： This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.
摘要翻译：本发明涉及功能性超活动光信号相关分子HFR1-DeltaN105，其中编码N末端105个氨基酸残基的核酸被缺失。 HFR1作为bHLH转录因子在植物色素A信号级联的一个子集中起作用，据报道COP1负调节。 HFR1-DeltaN105过表达植物的实验显示，N末端氨基酸的缺失使得HFR1在光形态发生如萌发和脱乙酰化中更具活性。此外，转基因植物在抑制下胚轴延伸方面表现出过敏光响应，依赖于光信号传导的另一阳性元件bZIP蛋白HY5。在HFR1-DeltaN105过表达植物中，终末的远红光反应和叶柄伸长被抑制。这些结果表明，HFR1的N末端区域负调节HFR1功能，HFR1-DeltaN105是多活性的。

4. 发明申请

US20050039225A1 Hyperactive light signal related molecule of HFR1-deltaN105 and transgenic plant thereof 失效
标题翻译： HFR1-δN105的多活性光信号相关分子及其转基因植物
公开(公告)号：US20050039225A1
公开(公告)日：2005-02-17
申请号：US10641100
申请日：2003-08-15
申请人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
发明人： Moon-Soo Soh , Pill-Soon Song , Ki-Young Yang
IPC分类号： C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/02
CPC分类号： C12N15/8237
摘要： This invention is about the functionally hyperactive light signal related molecule, HFR1-ΔN105, of which the nucleic acids that encode N-terminal 105 amino acid residues were deleted. HFR1 as a bHLH transcription factor functions in a subset of phytochrome A signaling cascade and it was reported to be regulated negatively by COP1. Experiments with a HFR1-ΔN105 overexpressing plant revealed that the deletion of N-terminal amino acids makes the HFR1 more active in photomorphogenic development such as germination and de-etiolation. In addition, the transgenic plants showed hypersensitive photo-responses in the inhibition of hypocotyl elongation, dependently on another positive element of light signaling, a bZIP protein, HY5. The end-of-day far-red light response and petiole elongation were suppressed in the HFR1-ΔN105 overexpressing plants. These results suggest that N-terminal region of HFR1 negatively regulate HFR1 function and that HFR1-ΔN105 is hyperactive.
摘要翻译：本发明涉及功能性超活动光信号相关分子HFR1-DeltaN105，其中编码N末端105个氨基酸残基的核酸被缺失。 HFR1作为bHLH转录因子在植物色素A信号级联的一个子集中起作用，据报道COP1负调节。 HFR1-DeltaN105过表达植物的实验显示，N末端氨基酸的缺失使得HFR1在光形态发生如萌发和脱乙酰化中更具活性。此外，转基因植物在抑制下胚轴延伸方面表现出过敏光响应，依赖于光信号传导的另一阳性元件bZIP蛋白HY5。在HFR1-DeltaN105过表达植物中，终末的远红光反应和叶柄伸长被抑制。这些结果表明，HFR1的N末端区域负调节HFR1功能，HFR1-DeltaN105是多活性的。

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