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1. 发明授权

US06861219B2 Preferential display 失效
标题翻译：优惠显示
公开(公告)号：US06861219B2
公开(公告)日：2005-03-01
申请号：US09961089
申请日：2001-09-24
申请人： Shahzi Iqbal , Robert Chin
发明人： Shahzi Iqbal , Robert Chin
IPC分类号： C12N15/10 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/1096 , C12Q1/6809 , C12Q2539/107 , C12Q2521/319
摘要： There is a tremendous need for high throughput gene expression technology which can efficiently and cheaply identify and accurately isolate different genes expressed between diseased and normal tissues for use in discovering new drugs. The present invention utilizes a combination of biomolecular chemistry methods to eliminate/degrade redundant sequences and fluorescence dye assay to identify these unique sequences from two cell or tissue populations. cDNA from normal or diseased cells or tissues are hybridized with the RNA of the complement normal or diseased cells or tissues. The hybridized cDNA/RNA is incubated with exonucleases, resulting in degradation of all but the single stranded RNA and DNA. RNA are then eliminated using RNase and the remaining DNA which are unique to the sample are amplified. This technique may be used to isolate differentially expressed genes or gene fragments and will provide a means to isolate and identify medium to low gene expressions that may otherwise be buried under gene “Noise”.
摘要翻译：对于高通量基因表达技术，需要高效，廉价地识别并准确分离用于发现新药的患病和正常组织之间表达的不同基因。本发明利用生物分子化学方法的组合来消除/降解冗余序列和荧光染料测定以鉴定来自两个细胞或组织群体的这些独特序列。来自正常或患病细胞或组织的cDNA与补体正常或患病细胞或组织的RNA杂交。杂交的cDNA / RNA与外切核酸酶一起孵育，导致除了单链RNA和DNA之外的所有蛋白质都被降解。然后使用RNase消除RNA，并扩增样品独特的剩余DNA。该技术可用于分离差异表达的基因或基因片段，并且将提供分离和鉴定可能被掩埋在基因“噪声”下的中低基因表达的方法。

2. 发明申请

US20100209298A1 Sample Processing System 审中-公开
标题翻译：样品处理系统
公开(公告)号：US20100209298A1
公开(公告)日：2010-08-19
申请号：US12542646
申请日：2009-08-17
申请人： Krishan L. Kalra , Wallace Chang , Ravishankar Melkote , Shahzi Iqbal
发明人： Krishan L. Kalra , Wallace Chang , Ravishankar Melkote , Shahzi Iqbal
IPC分类号： G01N35/10 , G01N1/28
CPC分类号： G01N1/28 , B01L3/508 , B01L3/5085 , B01L7/52 , B01L2200/0689 , B01L2200/147 , B01L2300/022 , B01L2300/024 , B01L2300/041 , B01L2300/0822 , G01N1/312 , G01N35/0099 , G01N35/109 , G01N2001/317 , G01N2035/00752 , Y10S901/31
摘要： In accordance with an embodiment of a system for handling and processing chemical and/or biological samples, a MicroChamber comprises a substrate, a reservoir formed on the substrate for receiving a chemical and/or biological sample, and an encoder such as a barcode or other suitable device. The encoder encodes information describing at least one characteristic of the substrate and/or reservoir.
摘要翻译：根据用于处理和处理化学和/或生物样品的系统的实施例，MicroChamber包括基板，形成在基板上的用于接收化学和/或生物样品的储存器，以及编码器，例如条形码或其它合适的装置。编码器编码描述衬底和/或贮存器的至少一个特性的信息。

3. 发明申请

US20060153736A1 Sample processing system 审中-公开
公开(公告)号：US20060153736A1
公开(公告)日：2006-07-13
申请号：US11373758
申请日：2006-03-09
申请人： Krishan Kalra , Wallace Chang , Ravishankar Melkote , Shahzi Iqbal
发明人： Krishan Kalra , Wallace Chang , Ravishankar Melkote , Shahzi Iqbal
IPC分类号： G01N31/22
CPC分类号： B01L3/50853 , B01L3/508 , B01L3/5085 , B01L3/545 , B01L7/52 , B01L2200/0689 , B01L2200/147 , B01L2300/021 , B01L2300/022 , B01L2300/024 , B01L2300/041 , B01L2300/0822 , B01L2300/089 , B01L2300/161 , G01N1/30 , G01N1/312 , G01N33/5302 , G01N35/0099 , G01N35/109 , G01N2001/317 , G01N2035/00138 , G01N2035/00752
摘要： In accordance with an embodiment of a system for handling and processing chemical and/or biological samples, a MicroChamber comprises a substrate, a reservoir formed on the substrate for receiving a chemical and/or biological sample, and an encoder such as a barcode or other suitable device. The encoder encodes information describing at least one characteristic of the substrate and/or reservoir.

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