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1. 发明授权

US4857453A Immunoassay device 失效
标题翻译：免疫测定装置
公开(公告)号：US4857453A
公开(公告)日：1989-08-15
申请号：US35562
申请日：1987-04-07
申请人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
发明人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
IPC分类号： G01N33/543 , G01N37/00
CPC分类号： G01N33/54366 , G01N33/54386 , Y10S435/805 , Y10S435/81 , Y10S436/807 , Y10S436/81
摘要： A device is disclosed for conducting an assay method. The device comprises a housing, means enclosed in the housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from the zone, one or more self-contained liquid reagents enclosed in the housing for conducting an assay method for the determination of an analyte in the sample, and means in the housing for introducing the sample into the device. Preferably, the self-contained reagents are liquid reagents which are contained in a breakable container. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay method are also disclosed.

2. 发明授权

US5137808A Immunoassay device 失效
标题翻译：免疫测定装置
公开(公告)号：US5137808A
公开(公告)日：1992-08-11
申请号：US388176
申请日：1989-07-31
申请人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
发明人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
IPC分类号： G01N33/543 , G01N37/00
CPC分类号： G01N33/54386 , G01N33/54366 , Y10S435/975 , Y10S436/807 , Y10S436/81
摘要： A device is disclosed for conducting an assay method. The device comprises a housing, means enclosed in the housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from the zone, one or more self-contained liquid reagents enclosed in the housing for conducting an assay method for the determination of an analyte in the sample, and means in the housing for introducing the sample into the device. Preferably, the self-contained reagents are liquid reagents which are contained in a breakable container. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay method are also disclosed.
摘要翻译：公开了用于进行测定方法的装置。该装置包括壳体，封闭在壳体中的装置，用于捕获区域中的特定装订对的构件并允许液体通过毛细管作用远离区域输送，一个或多个包含在壳体中的独立液体试剂用于进行用于测定样品中的分析物的测定方法，以及用于将样品引入该装置的壳体中的装置。优选地，独立的试剂是容纳在易碎容器中的液体试剂。本发明的装置用于测定怀疑含有分析物的样品中的分析物的测定方法。还公开了用于进行测定方法的试剂盒。

3. 发明授权

US4879215A Concentrating immunochemical test strip 失效
标题翻译：集中免疫化学试纸
公开(公告)号：US4879215A
公开(公告)日：1989-11-07
申请号：US164308
申请日：1988-03-04
申请人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
发明人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
IPC分类号： A61K39/00 , A61K39/395 , C12Q1/00 , G01N33/538 , G01N33/542 , G01N33/543 , G01N33/558
CPC分类号： G01N33/54313 , G01N33/538 , G01N33/542 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/966 , Y10S435/97 , Y10S435/973 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/818
摘要： A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample and a first member of a specific binding pair with an end portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The first member of a specific binding pair is capable of binding the analyte. The strip contains a second member of a specific binding pair integral therewith for concentrating and non-diffusively binding the first sbp member at a small situs on the strip separated from the end portion of the strip. The detectible signal is produced in relation to the presence of the analyte in the test solution. The test solution passes through the situs as the test solution traverses the bibulous material. After the test solution has been allowed to traverse at least a portion of the strip, the strip is contacted with a developer solution containing members of a signal producing system in a manner that provides contact of the developer solution with the small situs following its contact with the test solution. The strip is then contacted with any remaining members of the signal producing system. The detectible signal produced at the situs is then compared with the signal detectible at a portion of the strip other than the situs to determine the analyte in the sample. In one embodiment of the invention the signal produced at the small situs has a sharp-edged distinctive pattern that provides a sharp contrast to the signal produced at adjacent sites on the strip when analyte is present in the test solution.
摘要翻译：公开了一种用于确定怀疑含有分析物的样品中分析物的存在的方法和装置。该方法包括将含有样品和特定结合对的第一成员的测试溶液与能够通过毛细管作用穿过测试溶液的吸水材料条的端部接触。特异性结合对的第一个成员能够结合分析物。该带包含与其成一体的特定装订对的第二构件，用于在与带的端部分离的带上的小位置处浓缩和非扩散地结合第一sbp构件。相对于测试溶液中分析物的存在产生可检测信号。当测试溶液穿过吸水材料时，测试溶液通过位置。在测试溶液被允许穿过条带的至少一部分之后，条带与含有信号产生系统的显影剂溶液的方式接触，该显影剂溶液以与显影剂溶液接触之后提供显影剂溶液与小部位的接触的方式测试解决方案。然后将条带与信号产生系统的任何剩余部件接触。然后将在该位置处产生的可检测信号与除了位置之外的条带部分处可检测的信号进行比较，以确定样品中的分析物。在本发明的一个实施例中，在小区域产生的信号具有尖锐的特征图案，当分析物存在于测试溶液中时，与带上的相邻位置产生的信号形成鲜明的对比。

4. 发明授权

US4774191A Fluorescent conjugates bound to a support 失效
标题翻译：荧光共轭物与支持物结合
公开(公告)号：US4774191A
公开(公告)日：1988-09-27
申请号：US826177
申请日：1986-02-05
申请人： Pyare Khanna , Edwin F. Ullman
发明人： Pyare Khanna , Edwin F. Ullman
IPC分类号： C07C63/68 , C07D311/82 , C07J41/00 , G01N33/533 , G01N33/544
CPC分类号： C07J41/0033 , C07D311/82 , G01N33/533 , Y10S436/80 , Y10S436/805
摘要： Fluorescent antigen conjugates are provided comprising antigens covalently bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.
摘要翻译：提供荧光抗原缀合物，其包含与至少一种2,7-二脂肪族取代的9-苯基-6-羟基-3H-呫吨-3-酮共价结合的抗原，其中1-位和8-位是未取代的。还提供了在超过500nm的波长下吸收的新型荧光化合物，具有用于连接至抗原的活性官能团。最后，提供了分析血清抗原的方法，避免了血清干扰。

5. 发明授权

US4351760A Novel alkyl substituted fluorescent compounds and polyamino acid conjugates 失效
标题翻译：新型烷基取代荧光化合物和聚氨基酸共轭物
公开(公告)号：US4351760A
公开(公告)日：1982-09-28
申请号：US73158
申请日：1979-09-07
申请人： Pyare Khanna , Edwin F. Ullman
发明人： Pyare Khanna , Edwin F. Ullman
IPC分类号： G01N21/78 , C07C63/68 , C07D311/82 , C07H21/04 , C07J41/00 , C07K14/00 , C07K14/76 , C07K16/00 , C09B11/28 , C09K11/06 , C09K11/07 , G01N33/533 , A61K39/385 , A61K39/44 , C07G7/00
CPC分类号： C07J41/0088 , C07D311/82 , C07J41/0055 , G01N33/533 , Y10S435/961 , Y10S435/968 , Y10S530/806
摘要： Fluorescent antigen conjugates are provided comprising antigens covalently bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.
摘要翻译：提供荧光抗原缀合物，其包含与至少一种2,7-二脂肪族取代的9-苯基-6-羟基-3H-呫吨-3-酮共价结合的抗原，其中1-位和8-位是未取代的。还提供了在超过500nm的波长下吸收的新型荧光化合物，具有用于连接至抗原的活性官能团。最后，提供了分析血清抗原的方法，避免了血清干扰。

6. 发明授权

US4481136A Alkyl substituted fluorescent compounds and conjugates 失效
标题翻译：烷基取代的荧光化合物和共轭物
公开(公告)号：US4481136A
公开(公告)日：1984-11-06
申请号：US399506
申请日：1982-07-19
申请人： Pyare Khanna , Edwin F. Ullman
发明人： Pyare Khanna , Edwin F. Ullman
IPC分类号： C07C63/68 , C07D311/82 , C07J41/00 , G01N33/533 , A61K39/385 , C07G7/00
CPC分类号： C07J41/0088 , C07D311/82 , C07J41/0055 , G01N33/533 , Y10S435/968 , Y10S530/802 , Y10S530/806
摘要： Fluorescent antigen conjugates are provided comprising antigens covalantly bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.
摘要翻译：提供荧光抗原缀合物，其包含与至少一种2,7-二脂肪族取代的9-苯基-6-羟基-3H-呫吨-3-酮共价键合的抗原，其中所述1-位和8-位是未取代的。还提供了在超过500nm的波长下吸收的新型荧光化合物，具有用于连接至抗原的活性官能团。最后，提供了分析血清抗原的方法，避免了血清干扰。

7. 发明授权

US5716778A Concentrating immunochemical test device and method 失效
标题翻译：集中免疫化学检测装置及方法
公开(公告)号：US5716778A
公开(公告)日：1998-02-10
申请号：US351976
申请日：1989-05-15
申请人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
发明人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
IPC分类号： A61K39/00 , A61K39/395 , C12Q1/00 , G01N33/538 , G01N33/542 , G01N33/543 , G01N33/558
CPC分类号： G01N33/54313 , G01N33/538 , G01N33/542 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/966 , Y10S435/97 , Y10S435/973 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/818
摘要： A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample and a first member of a specific binding pair with an end portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The first member of a specific binding pair is capable of binding the analyte. The strip contains a second member of a specific binding pair integral therewith for concentrating and non-diffusively binding the first sbp member at a small situs on the strip separated from the end portion of the strip. The detectible signal is produced in relation to the presence of the analyte in the test solution. The test solution passes through the situs as the test solution traverses the bibulous material. After the test solution has been allowed to traverse at least a portion of the strip, the strip is contacted with a developer solution containing members of a signal producing system in a manner that provides contact of the developer solution with the small situs following its contact with the test solution. The strip is then contacted with any remaining members of the signal producing system. The detectible signal produced at the situs is then compared with the signal detectible at a portion of the strip other than the situs to determine the analyte in the sample. In one embodiment of the invention the signal produced at the small situs has a sharp-edged distinctive pattern that provides a sharp contrast to the signal produced at adjacent sites on the strip when analyte is present in the test solution.
摘要翻译：公开了一种用于确定怀疑含有分析物的样品中分析物的存在的方法和装置。该方法包括将含有样品和特定结合对的第一成员的测试溶液与能够通过毛细管作用穿过测试溶液的吸水材料条的端部接触。特异性结合对的第一个成员能够结合分析物。该带包含与其成一体的特定装订对的第二构件，用于在与带的端部分离的带上的小位置处浓缩和非扩散地结合第一sbp构件。相对于测试溶液中分析物的存在产生可检测信号。当测试溶液穿过吸水材料时，测试溶液通过位置。在测试溶液被允许穿过条带的至少一部分之后，条带与含有信号产生系统的显影剂溶液的方式接触，该显影剂溶液以与显影剂溶液接触之后提供显影剂溶液与小部位的接触的方式测试解决方案。然后将条带与信号产生系统的任何剩余部件接触。然后将在该位置处产生的可检测信号与除了位置之外的条带部分处可检测的信号进行比较，以确定样品中的分析物。在本发明的一个实施例中，在小部位产生的信号具有尖锐的特征图案，当分析物存在于测试溶液中时，与带上的相邻位置产生的信号形成鲜明的对比。

8. 发明授权

US4740468A Concentrating immunochemical test device and method 失效
标题翻译：集中免疫化学检测装置及方法
公开(公告)号：US4740468A
公开(公告)日：1988-04-26
申请号：US701464
申请日：1985-02-14
申请人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
发明人： Litai Weng , David Calderhead , Pyare Khanna , Edwin F. Ullman
IPC分类号： A61K39/00 , A61K39/395 , C12Q1/00 , G01N33/538 , G01N33/542 , G01N33/543 , G01N33/558 , G01N33/53 , G01N33/535
CPC分类号： G01N33/54313 , G01N33/538 , G01N33/542 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/966 , Y10S435/97 , Y10S435/973 , Y10S435/975 , Y10S436/81 , Y10S436/814 , Y10S436/818
摘要： A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample and a first member of a specific binding pair with an end portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The first member of a specific binding pair is capable of binding the analyte. The strip contains a second member of a specific binding pair integral therewith for concentrating and non-diffusively binding the first sbp member at a small situs on the strip separated from the end portion of the strip. The detectible signal is produced in relation to the presence of the analyte in the test solution. The test solution passes through the situs as the test solution traverses the bibulous material. After the test solution has been allowed to traverse at least a portion of the strip, the strip is contacted with a developer solution containing members of a signal producing system in a manner that provides contact of the developer solution with the small situs following its contact with the test solution. The strip is then contacted with any remaining members of the signal producing system. The detectible signal produced at the situs is then compared with the signal detectible at a portion of the strip other than the situs to determine the analyte in the sample.
摘要翻译：公开了一种用于确定怀疑含有分析物的样品中分析物的存在的方法和装置。该方法包括将含有样品和特定结合对的第一成员的测试溶液与能够通过毛细管作用穿过测试溶液的吸水材料条的端部接触。特异性结合对的第一个成员能够结合分析物。该带包含与其成一体的特定装订对的第二构件，用于在与带的端部分离的带上的小位置处浓缩和非扩散地结合第一sbp构件。相对于测试溶液中分析物的存在产生可检测信号。当测试溶液穿过吸水材料时，测试溶液通过位置。在测试溶液被允许穿过条带的至少一部分之后，条带与含有信号产生系统的显影剂溶液的方式接触，该显影剂溶液以与显影剂溶液接触之后提供显影剂溶液与小部位的接触的方式测试解决方案。然后将条带与信号产生系统的任何剩余部件接触。然后将在该位置处产生的可检测信号与除了位置之外的条带部分处可检测的信号进行比较，以确定样品中的分析物。

9. 发明授权

US4588697A Method for performing fluorescent protein binding assay employing novel alkyl substituted fluorescent compounds and conjugates 失效
标题翻译：使用新的烷基取代的荧光化合物和共轭物进行荧光蛋白结合测定的方法
公开(公告)号：US4588697A
公开(公告)日：1986-05-13
申请号：US664121
申请日：1984-10-23
申请人： Pyare Khanna , Edwin F. Ullman
发明人： Pyare Khanna , Edwin F. Ullman
IPC分类号： C07C63/68 , C07D311/82 , C07J41/00 , G01N33/533 , G01N33/543 , G01N33/545 , G01N33/549
CPC分类号： C07D311/82 , C07J41/0055 , C07J41/0088 , G01N33/533 , Y10S436/80 , Y10S530/807
摘要： Fluorescent antigen conjugates are provided comprising antigens covalantly bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.
摘要翻译：提供荧光抗原缀合物，其包含与至少一种2,7-二脂肪族取代的9-苯基-6-羟基-3H-呫吨-3-酮共价键合的抗原，其中所述1-位和8-位是未取代的。还提供了在超过500nm的波长下吸收的新型荧光化合物，具有用于连接至抗原的活性官能团。最后，提供了分析血清抗原的方法，避免了血清干扰。

10. 发明授权

US4318846A Novel ether substituted fluorescein polyamino acid compounds as fluorescers and quenchers 失效
标题翻译：新型醚取代的荧光素聚氨基酸化合物作为荧光剂和猝灭剂
公开(公告)号：US4318846A
公开(公告)日：1982-03-09
申请号：US73163
申请日：1979-09-07
申请人： Pyare Khanna , Edwin F. Ullman
发明人： Pyare Khanna , Edwin F. Ullman
IPC分类号： C07C41/26 , C07C43/23 , C07D311/82 , C07D493/10 , C09B11/08 , C09B11/26 , G01N33/533 , A61K39/385 , A61K39/44 , C07G7/00
CPC分类号： C07D493/10 , C07C41/26 , C07D311/82 , C09B11/08 , C09B11/26 , G01N33/533 , Y10S435/961 , Y10S435/968 , Y10S530/806
摘要： Diether symmetrically substituted fluoresceins are provided having at least one anionic group and a linking functionality. Depending upon the site of substitution, the compounds can be used as fluorescers absorbing at wavelengths in excess of 500 nm or as quenchers, absorbing at wavelengths in excess of 500 nm and exhibiting substantially no fluorescence. The compounds find wide application, particularly as labels in fluorescent immunoassays.
摘要翻译： Diether对称取代的荧光素具有至少一个阴离子基团和连接官能团。取决于取代的位置，化合物可以用作在超过500nm的波长下吸收的荧光物质，或者作为猝灭剂使用，其吸收波长超过500nm并且基本上不显示荧光。该化合物广泛应用，特别是作为荧光免疫测定中的标记物。

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