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    • 1. 发明授权
    • Method and apparatus for ultrasonic lysis of biological cells
    • 生物细胞超声裂解的方法和装置
    • US06686195B1
    • 2004-02-03
    • US09937992
    • 2001-12-13
    • Bruno ColinPhilippe CleuziatPatrick BroyerClaude MabilatSandra Incardona
    • Bruno ColinPhilippe CleuziatPatrick BroyerClaude MabilatSandra Incardona
    • C12M133
    • B01J19/10B06B3/00C12M45/02
    • An apparatus including at least one sonotrode (2) designed to generate ultrasound of variable power in at least one biological sample (5) containing cells to be lysed, the sample (5) being contained in at least one receptacle (4 or 10) such that the sonotrode (2) is in direct contact with the receptacle(s) (4). Also disclosed is a method for using ultrasound to lyse a biological sample (5) contained in a receptacle (4), which includes placing the receptacle (4) in direct contact with the sonotrode (2), and activating the sonotrode (2) for long enough to lyse the cells in the sample (5) but preserve the DNA and/or RNA molecules released for subsequent operations, e.g. amplification. The invention is particularly applicable in the discipline of molecular biology.
    • 一种装置,包括至少一个超声波发生器(2),其被设计成在至少一个含有待裂解细胞的生物样品(5)中产生可变功率的超声波,所述样品(5)包含在至少一个容器(4或10)中, 超声波发生器(2)与容器(4)直接接触。 还公开了一种使用超声波来溶解包含在容器(4)中的生物样品(5)的方法,其包括将容器(4)放置在与超声焊极(2)直接接触的位置,并且激活超声焊极(2)以用于 足以裂解样品(5)中的细胞,但保留释放用于后续操作的DNA和/或RNA分子,例如 放大。 本发明特别适用于分子生物学学科。
    • 3. 发明授权
    • DNA fragments, probes and amplification primers of the 65 kD antigen of
mycobacteria
    • 分子细菌65 kD抗原的DNA片段,探针和扩增引物
    • US5589585A
    • 1996-12-31
    • US105168
    • 1993-08-12
    • Claude MabilatJean-Claude Pechere
    • Claude MabilatJean-Claude Pechere
    • A61K39/00C07K14/35C12N15/31C12P21/08C12Q1/68C07H21/04
    • C12Q1/689C07K14/35A61K39/00
    • Nucleotide fragment of DNA, whose nucleotide sequence is included in the gene of the species of the genus Mycobacterium, coding for the 65-kD mycobacterial antigen, containing regions which are homologous in practically all species of the genus Mycobacterium, and at least one species-specific variable region, characterized in that said fragment is chosen from fragments whose nucleotide sequences possess at least 70% homology, and preferably at least 85% homology, with a predetermined sequence or its complementary sequence, said predetermined sequence beginning at nucleotide 438 and ending at nucleotide 751 of said gene coding for said antigen of all species of mycobacteria except for the species M. tuberculosis, M. bovis BCG, M. avium, M. paratuberculosis, M. fortuitum, M. malmoense, M. leprae, M. kansaii and M. marinurn.
    • DNA的核苷酸片段,其核苷酸序列包括在分枝杆菌属的种类的基因中,编码65-kD分枝杆菌抗原,其含有几乎所有分枝杆菌属物种中同源的区域,以及至少一种物种 - 特征在于所述片段选自其核苷酸序列与预定序列或其互补序列具有至少70%同源性,优选至少85%同源性的片段,所述预定序列从核苷酸438开始并以 编码所有分枝杆菌种类的所述抗原的所述基因的核苷酸751除了结核分枝杆菌,牛分枝杆菌BCG,鸟分枝杆菌,副结核分枝杆菌,马铃薯,马尔莫森,麻疯树,马萨诸塞州 和M. marinurn。