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1. 发明申请

US20050170446A1 Sandwich assay and kit 审中-公开
标题翻译：三明治测定和试剂盒
公开(公告)号：US20050170446A1
公开(公告)日：2005-08-04
申请号：US10511711
申请日：2003-04-29
申请人： Niklas Ahlborg , Per-Ake Nygren
发明人： Niklas Ahlborg , Per-Ake Nygren
IPC分类号： G01N33/566 , G01N33/53 , G01N33/531 , G01N33/537 , G01N33/543 , G01N33/569
CPC分类号： G01N33/543 , G01N33/54306 , G01N33/56938 , G01N33/56944
摘要： A sandwich assay method for detecting the presence of a target molecule in a sample comprising a complex biological fluid is provided. The assay comprises providing a first affinity ligand with affinity for the target molecule, which affinity ligand is capable of being immobilized to a solid support; applying the sample in such a way that binding of a target molecule, if present in the sample, to the first affinity ligand is enabled; applying a second affinity ligand with affinity for the target molecule, the application enabling binding of the second affinity ligand to the target molecule; removing second affinity ligand not bound to target molecule; and detecting the presence of the second affinity ligand, such presence being an indicator of the presence of a target molecule in the sample. The first affinity ligand is immobilized to the solid support at any stage before said detection, and at least one of the first and second affinity ligands is an affinity ligand other than an antibody. The invention also provides a kit for use in the method. The kit comprises: a first affinity ligand with affinity for the target molecule and capable of being immobilized to a slid support; a second affinity ligand with affinity for the target molecule, the presence of which ligand is detectable; and a solid support to which the first affinity ligand is capable of being immobilized. In he kit, at least one of the first and second affinity ligands is an affinity ligand other than an antibody.
摘要翻译：提供了一种用于检测包含复杂生物流体的样品中靶分子存在的夹心测定方法。该测定包括提供对靶分子具有亲和力的第一亲和配体，该亲和配体能够固定在固体支持物上; 以如下方式施用样品：将目标分子（如果存在于样品中）与第一亲和配体的结合起作用; 对靶分子施加具有亲和力的第二亲和配体，该应用使第二亲和配体与靶分子结合; 去除不与靶分子结合的第二亲和配体; 并检测第二亲和配体的存在，这种存在是样品中靶分子存在的指标。在所述检测之前的任何阶段将第一亲和配体固定在固体支持物上，并且第一和第二亲和配体中的至少一个是抗体以外的亲和配体。本发明还提供了一种用于该方法的试剂盒。试剂盒包括：对靶分子具有亲和力并能够固定在滑动支持物上的第一亲和配体; 对靶分子具有亲和性的第二亲和配体，可检测哪种配体的存在; 和第一亲和配体能够固定的固体载体。在试剂盒中，第一和第二亲和配体中的至少一个是抗体以外的亲和配体。

2. 发明授权

US6130091A Method for obtaining a peptide derived from the respiratory syncytial virus polypeptide and bacterium expressing it 失效
标题翻译：获得源自呼吸道合胞病毒多肽的肽和表达它的细菌的方法
公开(公告)号：US6130091A
公开(公告)日：2000-10-10
申请号：US836504
申请日：1997-07-01
申请人： Hans Binz , Thien Nguyen Ngoc , Stefan Stahl , Mathias Uhlen , Per Ake Nygren
发明人： Hans Binz , Thien Nguyen Ngoc , Stefan Stahl , Mathias Uhlen , Per Ake Nygren
IPC分类号： C12N15/09 , A61K38/00 , A61K39/00 , A61K39/155 , A61P31/12 , C07K14/135 , C07K14/31 , C12N1/21 , C12N15/45 , C12P21/02 , C12R1/44 , C12N7/00 , C12N1/00 , C12N15/00
CPC分类号： C07K14/005 , C07K14/31 , A61K38/00 , A61K39/00 , C07K2319/00 , C12N2760/18522 , Y10S435/882
摘要： A method for obtaining a Staphylococcus carnosus bacterium expressing at its membrane surface a recombinant polypeptide derived from RSV protein G, said polypeptide having a sequence which is a modification of SEQ ID No. 1 and SEQ ID No. 2 (sequence encompassed between residues 130 and 230 of RSV A and RSV B protein G respectively) wherein at least one of position 44 and 57 is serine is disclosed.
摘要翻译： PCT No.PCT / FR95 / 01465 Sec。 371日期1997年7月1日 102（e）日期1997年7月1日PCT提交1995年11月7日PCT公布。公开号WO96 / 14418 PCT 日期1996年5月17日一种用于获得在其膜表面表达来自RSV蛋白G的重组多肽的葡萄球菌属细菌的方法，所述多肽具有作为SEQ ID No.1和SEQ ID No.2的修饰（序列包括在分别为RSV A和RSV B蛋白G的残基130和230之间），其中位置44和57中的至少一个是丝氨酸。

3. 发明授权

US06955877B1 In vitro selection and optional identification of polypeptides using solid support carriers 失效
标题翻译：使用固体支持载体的体外选择和多肽的任选鉴定
公开(公告)号：US06955877B1
公开(公告)日：2005-10-18
申请号：US10031910
申请日：2000-07-20
申请人： Per-Ake Nygren , Mathias Uhlen , Olof Nord
发明人： Per-Ake Nygren , Mathias Uhlen , Olof Nord
IPC分类号： C12N15/09 , C07K1/04 , C07K19/00 , C12N15/10 , C12Q1/68 , C07K1/00
CPC分类号： C12Q1/6811 , C12N15/1075
摘要： A method for the selection of one or more desired polypeptides includes cell free expression of nucleic acid molecules immobilized on a solid support system to produce polypeptides. The solid support carrying system is for biospecific interaction with at least the desired polypeptide or a molecule attached thereto. The method also includes separation of the solid support carrying both the desired polypeptide and the nucleic acid encoding it. Finally, the method optionally includes recovery of the nucleic acid and/or the desired polypeptide, and molecular libraries for use in these methods.
摘要翻译：用于选择一种或多种所需多肽的方法包括固定在固体支持体系上以产生多肽的核酸分子的无细胞表达。固体支持载体系统用于与至少所需多肽或与其连接的分子的生物特异性相互作用。该方法还包括携带所需多肽和编码其的核酸的固体支持物的分离。最后，该方法任选包括回收核酸和/或所需多肽，以及用于这些方法的分子文库。

4. 发明申请

US20090098590A1 ENDOTOXIN ANALYSIS 失效
标题翻译：内毒素分析
公开(公告)号：US20090098590A1
公开(公告)日：2009-04-16
申请号：US12092451
申请日：2006-11-02
申请人： Gen Larsson , Per-Ake Nygren
发明人： Gen Larsson , Per-Ake Nygren
IPC分类号： C12Q1/37 , G01N33/00
CPC分类号： C12Q1/04 , G01N2800/26
摘要： The present invention relates to a method for detecting the presence or non-presence of an endotoxin, characterized in that an OmpT protein is brought into contact with a sample suspected of containing an endotoxin and the protease activity of the OmpT protein is assayed. It also relates to a method for detecting early onset of septicaemia using the inventive method and a kit for performing the method.
摘要翻译：本发明涉及一种用于检测内毒素的存在或不存在的方法，其特征在于使OmpT蛋白与怀疑含有内毒素的样品接触，并测定OmpT蛋白的蛋白酶活性。它还涉及使用本发明的方法和用于执行该方法的试剂盒检测败血病早期发作的方法。

5. 发明授权

US06558924B1 Recombinant expression of insulin C-peptide 失效
标题翻译：胰岛素C肽的重组表达
公开(公告)号：US06558924B1
公开(公告)日：2003-05-06
申请号：US09485286
申请日：2000-02-07
申请人： Stefan Stahl , Mathias Uhlen , Per-Ake Nygren , Per Jonasson
发明人： Stefan Stahl , Mathias Uhlen , Per-Ake Nygren , Per Jonasson
IPC分类号： C12N1500
CPC分类号： C12N15/62 , C07K14/62 , C07K2319/00 , C07K2319/50 , C07K2319/705 , C07K2319/75
摘要： The present invention provides a method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of the insulin C-peptide, and cleaving the expressed polypeptide to release single copies of the insulin C-peptide. Also provided are nucleic acid molecules, expression vectors and host cells, for use in such a method and the multimeric insulin C-peptide polypeptide expressed and cleaved in such a method.
摘要翻译：本发明提供了一种生产胰岛素C肽的方法，其包括在宿主细胞中表达包含胰岛素C-肽的多个拷贝的多聚体多肽，并切割表达的多肽以释放胰岛素C肽的单拷贝。还提供了用于这种方法的核酸分子，表达载体和宿主细胞，并且以这种方法表达和切割多聚胰岛素C肽多肽。

6. 发明申请

US20060035265A1 In vitro selection and optional indentification of polypeptides using solid support carriers 审中-公开
标题翻译：使用固体支持载体的多肽的体外选择和任选的鉴定
公开(公告)号：US20060035265A1
公开(公告)日：2006-02-16
申请号：US11242588
申请日：2005-10-03
申请人： Per-Ake Nygren , Mathias Uhlen , Olof Nord
发明人： Per-Ake Nygren , Mathias Uhlen , Olof Nord
IPC分类号： C40B40/08 , C40B40/10
CPC分类号： C12Q1/6811 , C12N15/1075
摘要： The present invention relates to a nucleic acid molecule or a polypeptide that is selected from a method that includes cell-free expression of nucleic acid molecules immobilized on a sold support system. The present invention also relates to a molecular library that has a solid support system having a plurality of nucleic acid molecules immobilized thereon.
摘要翻译：本发明涉及一种核酸分子或多肽，其选自包含固定在已售出的支持体系上的核酸分子的无细胞表达的方法。本发明还涉及具有固定有多个核酸分子的固体支持体系的分子文库。

7. 发明授权

US6149911A Method for enhancing the immunogenicity of an immunogenic compound or hapten, and use thereof for preparing vaccines 失效
标题翻译：增强免疫原性化合物或半抗原的免疫原性的方法及其制备疫苗的用途
公开(公告)号：US6149911A
公开(公告)日：2000-11-21
申请号：US836501
申请日：1997-07-01
申请人： Hans Binz , Thien Nguyen Ngoc , Christine Andreoni , Per Ake Nygren , Stefan Stahl , Mathias Uhlen
发明人： Hans Binz , Thien Nguyen Ngoc , Christine Andreoni , Per Ake Nygren , Stefan Stahl , Mathias Uhlen
IPC分类号： C12N15/09 , A61K39/00 , A61K39/002 , A61K39/02 , A61K39/12 , A61K39/155 , A61K39/29 , A61K39/385 , A61K39/39 , A61K48/00 , C07K14/135 , C07K14/315 , C12N15/31 , C07K1/10 , C07K19/00 , C12N15/62
CPC分类号： C07K14/005 , A61K39/12 , A61K39/155 , A61K39/385 , A61K2039/55505 , A61K2039/55566 , A61K2039/6037 , A61K2039/6068 , C12N2760/18522 , C12N2760/18534
摘要： The present invention relates to a process for improving the immunogenicity of an immunogen, an antigen or a hapten, when it is administered to a host, independently of the mode of administration, characterized in that the said antigen or hapten is coupled covalently to a support molecule in order to form a complex, and in that this support molecule is a polypeptide fragment which is able to bind specifically to mammalian serum albumin. The invention also relates to the use, as a medicament, of the product which can be obtained in this way.
摘要翻译： PCT No.PCT / FR95 / 01466 Sec。 371日期1997年7月1日 102（e）日期1997年7月1日PCT提交1995年11月7日PCT公布。出版物WO96 / 14416 PCT 日期1996年5月17日本发明涉及一种提供免疫原，抗原或半抗原的免疫原性的方法，当其施用于宿主时，与给药方式无关，其特征在于所述抗原或半抗原偶联共价于支持分子以形成复合物，并且因为该支持分子是能够特异性结合哺乳动物血清白蛋白的多肽片段。本发明还涉及以这种方式获得的产品作为药物的用途。

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