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1. 发明申请

US20100028941A1 Method for Multiparallel Construction of Host/Vector-Systems for Expression of Proteins 审中-公开
标题翻译：用于表达蛋白质的宿主/载体系统的多平行构建方法
公开(公告)号：US20100028941A1
公开(公告)日：2010-02-04
申请号：US12297879
申请日：2007-04-23
申请人： Gen Larsson
发明人： Gen Larsson
IPC分类号： C12P21/00 , C12N15/70 , C12N1/21
CPC分类号： C12N1/20 , C12N15/70 , C12P21/02
摘要： The present invention relates to a multiparallel method for construction of host/vector-systems, comprising transforming a plurality of host strains, wherein substantially all host strains have deleterious mutations in at least one uptake system for a critical substrate needed for the growth of said host strain, with a plurality of vectors encoding at least one protein, and expressing said recombinant protein in said host strains. It further relates to host/vector systems obtainable by the method, to kits comprising the host strains and optionally the vectors, and to use of produced host/vector systems for protein expression. The method is exemplified using an E. coli strain with deleterious mutation in both ptsG and ptsM.
摘要翻译：本发明涉及用于构建宿主/载体系统的多平行方法，包括转化多种宿主菌株，其中基本上所有宿主菌株在用于所述宿主生长所需的关键底物的至少一种摄取系统中具有有害突变菌株，编码至少一种蛋白质的多种载体，并在所述宿主菌株中表达所述重组蛋白质。它还涉及通过该方法可获得的宿主/载体系统，包含宿主菌株和任选的载体的试剂盒，以及使用产生的宿主/载体系统进行蛋白质表达。使用在ptsG和ptsM中具有有害突变的大肠杆菌菌株来举例说明该方法。

2. 发明授权

US07998697B2 Endotoxin analysis 失效
标题翻译：内毒素分析
公开(公告)号：US07998697B2
公开(公告)日：2011-08-16
申请号：US12092451
申请日：2006-11-02
申请人： Gen Larsson , Per-Åke Nygren
发明人： Gen Larsson , Per-Åke Nygren
IPC分类号： C12Q1/42
CPC分类号： C12Q1/04 , G01N2800/26
摘要： The present invention relates to a method for detecting the presence or non-presence of an endotoxin, characterized in that an OmpT protein is brought into contact with a sample suspected of containing an endotoxin and the protease activity of the OmpT protein is assayed. It also relates to a method for detecting early onset of septicaemia using the inventive method and a kit for performing the method.
摘要翻译：本发明涉及一种用于检测内毒素的存在或不存在的方法，其特征在于使OmpT蛋白与怀疑含有内毒素的样品接触，并测定OmpT蛋白的蛋白酶活性。它还涉及使用本发明的方法和用于执行该方法的试剂盒检测败血病早期发作的方法。

3. 发明申请

US20090098590A1 ENDOTOXIN ANALYSIS 失效
标题翻译：内毒素分析
公开(公告)号：US20090098590A1
公开(公告)日：2009-04-16
申请号：US12092451
申请日：2006-11-02
申请人： Gen Larsson , Per-Ake Nygren
发明人： Gen Larsson , Per-Ake Nygren
IPC分类号： C12Q1/37 , G01N33/00
CPC分类号： C12Q1/04 , G01N2800/26
摘要： The present invention relates to a method for detecting the presence or non-presence of an endotoxin, characterized in that an OmpT protein is brought into contact with a sample suspected of containing an endotoxin and the protease activity of the OmpT protein is assayed. It also relates to a method for detecting early onset of septicaemia using the inventive method and a kit for performing the method.
摘要翻译：本发明涉及一种用于检测内毒素的存在或不存在的方法，其特征在于使OmpT蛋白与怀疑含有内毒素的样品接触，并测定OmpT蛋白的蛋白酶活性。它还涉及使用本发明的方法和用于执行该方法的试剂盒检测败血病早期发作的方法。

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