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1. 发明授权

US5403451A Method and apparatus for pulsed electrochemical detection using polymer electroactive electrodes 失效
标题翻译：使用聚合物电活性电极进行脉冲电化学检测的方法和装置
公开(公告)号：US5403451A
公开(公告)日：1995-04-04
申请号：US206136
申请日：1994-03-04
申请人： John M. Riviello , Gordon Wallace , Omowunmi A. Sadik
发明人： John M. Riviello , Gordon Wallace , Omowunmi A. Sadik
IPC分类号： G01N27/447 , G01N27/327 , G01N27/38 , G01N27/416 , G01N27/42 , G01N27/48 , G01N30/64 , G01N30/78 , G01N27/26
CPC分类号： G01N27/3277
摘要： In a first embodiment, a target analyte in solution is detected by exposing the solution to an electrode that includes a conducting electroactive polymer to which a periodic alternating voltage is coupled. Upon exposure to the analyte, an electrode characteristic is varied, which variation is detected by measuring electrode current as a function of time and as a function of the periodic alternating voltage. The alternating voltage waveform has an oxidizing time period and a reduction time period, which periods and waveform duty cycle may be controlled to enhance electrode sensitivity, selectivity, and to substantially eliminate electrode fouling and data hysteresis. In a second embodiment, a receptor is bound to the electrode, to which is coupled an alternating voltage waveform that permits a mating target substance to reversibly bind to the receptor such that measurement of electrode current provides a measure of such reversible binding. The second embodiment is especially useful for detecting antibodies, antigens, haptens, DNA, RNA, and enzymes. Either embodiment may be used for detection in flow-through electrochemical cells, flow injection analysis, liquid, and ion chromatography, as well as in capillary electrophoresis.
摘要翻译：在第一实施方案中，溶液中的目标分析物通过将溶液暴露于包括连接有周期性交流电压的导电电活性聚合物的电极来检测。在暴露于分析物时，改变电极特性，通过测量电极电流作为时间的函数并且作为周期性交变电压的函数来检测该变化。交流电压波形具有氧化时间段和减少时间周期，可以控制这些周期和波形占空比以增强电极灵敏度，选择性，并且基本上消除电极结垢和数据滞后。在第二实施方案中，受体结合到电极，其耦合交流电压波形，其允许配对目标物质可逆地结合受体，使得电极电流的测量提供了这种可逆结合的量度。第二个实施方案对于检测抗体，抗原，半抗原，DNA，RNA和酶特别有用。两种实施方案可用于流通电化学电池中的检测，流动注射分析，液体和离子色谱法以及毛细管电泳。

2. 发明申请

US20130089876A1 CAPILLARY BIOSENSOR SYSTEM AND ITS METHOD OF USE 审中-公开
标题翻译：毛细生物传感器系统及其使用方法
公开(公告)号：US20130089876A1
公开(公告)日：2013-04-11
申请号：US13640896
申请日：2011-04-19
申请人： Omowunmi A. Sadik , Qiong Wang , Paul A. Blythe, SR.
发明人： Omowunmi A. Sadik , Qiong Wang , Paul A. Blythe, SR.
IPC分类号： G01N33/566 , G01N33/53
CPC分类号： G01N33/566 , G01N21/6428 , G01N21/7703 , G01N33/5302 , G01N2021/0346 , G01N2021/7786 , G01N2201/0221
摘要： A portable biosensor system includes at least one capillary tube extending longitudinally along a major axis between a proximal inlet end and a distal end. The at least one capillary tube has an interior surface coated with a capture material and forms a waveguide. At least one collimated light emitting diode is disposed proximate and perpendicular to the major axis of the at least one capillary tube and is positioned relative to the at least one capillary tube so that energy enters the at least one capillary tube from its exterior along the entire length of the at least one capillary tube to project a line of energy along substantially the entire longitudinal extent of the at least one capillary tube. A photosensor is disposed proximate the distal end of the at least one capillary tube for receiving emissive radiation therefrom. The photosensor generates an output voltage (or, optical output) representative of the emissive radiation, and a means for measuring the output voltage is provided. Also disclosed is a method of detecting target molecules in a sample using the biosensor of the present invention.
摘要翻译：便携式生物传感器系统包括沿近端入口端和远端之间的长轴纵向延伸的至少一个毛细管。所述至少一个毛细管具有涂覆有捕获材料并形成波导的内表面。至少一个准直发光二极管被设置为接近和垂直于至少一个毛细管的长轴并且相对于至少一个毛细管定位，使得能量沿着整个外部从其外部进入至少一个毛细管所述至少一个毛细管的长度沿所述至少一个毛细管的基本上整个纵向范围投射能量线。光传感器设置在至少一个毛细管的远端附近，用于从其接收发射辐射。光传感器产生表示发射辐射的输出电压（或光输出），并且提供用于测量输出电压的装置。还公开了使用本发明的生物传感器检测样品中的靶分子的方法。

3. 发明授权

US07708944B1 Ultra-sensitive, portable capillary sensor 有权
标题翻译：超灵敏便携式毛细管传感器
公开(公告)号：US07708944B1
公开(公告)日：2010-05-04
申请号：US11451842
申请日：2006-06-13
申请人： Omowunmi A. Sadik , Jason Karasinski
发明人： Omowunmi A. Sadik , Jason Karasinski
IPC分类号： G01N21/00
CPC分类号： G01N33/5302 , B01L3/5027 , G01N21/645 , G01N2021/0346
摘要： A portable, lightweight, rugged, easy-to-operate biosensor useful for rapidly detecting cells, viruses, antibodies, and other proteins. A capillary tube has a capture antibody immobilized on its interior surface. The specific capture antibody is selected based upon a desired target analyte to be detected. A sample potentially containing the target antigen is introduced into the capillary tube. Thereafter, a second antibody labeled with a fluorescent dye is introduced. Upon excitation by electromagnetic energy, typically supplied by a laser, the fluorescence of the sample is captured and analyzed. The apparatus is extremely compact and rugged making it ideal for field use. In addition, accurate results may be obtained by relatively unskilled operators directly from a self-contained readout. Optionally, an external device (e.g., a computer) may be connected to the apparatus via an optional interface. The analysis time provided by the biosensor system of the invention is shorter than has heretofore been possible.
摘要翻译：便携式，轻便，坚固，易于操作的生物传感器，可用于快速检测细胞，病毒，抗体和其他蛋白质。毛细管具有固定在其内表面上的捕获抗体。基于要检测的所需目标分析物选择特异性捕获抗体。将潜在含有目标抗原的样品引入毛细管中。此后，引入用荧光染料标记的第二抗体。在通常由激光供应的电磁能量激发时，捕获并分析样品的荧光。该设备非常紧凑和坚固，使其成为现场使用的理想选择。此外，相对不熟练的操作者可以直接从自包含的读数获得准确的结果。可选地，可以经由可选接口将外部设备（例如，计算机）连接到设备。由本发明的生物传感器系统提供的分析时间比以前更短。

4. 发明授权

US06602989B1 Synthesis, characterization, and application of pyridylazo bioconjugates as diagnostic and therapeutic agents 有权
标题翻译：吡啶偶氮生物缀合物作为诊断和治疗剂的合成，表征和应用
公开(公告)号：US06602989B1
公开(公告)日：2003-08-05
申请号：US09573993
申请日：2000-05-17
申请人： Omowunmi A. Sadik , Hongwu Xu
发明人： Omowunmi A. Sadik , Hongwu Xu
IPC分类号： C07K1710
CPC分类号： G01N33/5308 , A61K51/088 , Y10S436/804
摘要： The detection of gallium in biological samples is required due to its role in the diagnosis of tumor and for possible treatment of malignancies. However, the use of purely instrumental techniques is unsuitable for detection of low levels of gallium in biological matrices. New protein conjugates have been synthesized based on 4-(2-pyridylazo) ligands. The conjugates detect gallium in biological matrices using a non antibody-based sandwich assay format. The recovery level is between 97-101.3 with a relative standard deviation of less than 5%. The assay results in a detection limit of 5×10−8 M and a remarkable selectivity for gallium(III) relative to other metals investigated. The new method provides adequate accuracy for gallium applicable for animal physiology and clinical toxicology.
摘要翻译：由于其在肿瘤诊断和可能的恶性肿瘤治疗中的作用，需要检测生物样品中的镓。然而，纯工具技术的使用不适用于检测生物基质中低含量的镓。已经基于4-（2-吡啶基偶氮）配体合成了新的蛋白质缀合物。共轭物使用非抗体为基础的夹心测定形式检测生物基质中的镓。恢复水平在97-101.3之间，相对标准偏差小于5％。该测定结果的检测限为5×10 -8 M，并且相对于所研究的其它金属，镓（III）具有显着的选择性。该新方法为适用于动物生理学和临床毒理学的镓提供了足够的准确性。

5. 发明申请

US20090103084A1 OXYGEN SENSOR FOR USE IN HARSH, ESPECIALLY HIGH, pH ENVIRONMENTS 审中-公开
标题翻译： OXYGEN传感器用于海水，特别高的pH环境
公开(公告)号：US20090103084A1
公开(公告)日：2009-04-23
申请号：US12259819
申请日：2008-10-28
申请人： Omowunmi A. SADIK , Hongwu XU
发明人： Omowunmi A. SADIK , Hongwu XU
IPC分类号： G01J3/30
CPC分类号： G01N21/8507 , G01N21/643 , G01N21/7703 , G01N2021/6432 , G01N2021/6434 , G01N2021/6439 , G01N2021/6484 , G01N2021/7786
摘要： An oxygen sensor usable and accurate in solutions of up to pH 14 is featured. The sensor is fabricated by immobilizing a layer of polystyrene mixed with a fluorophore (e.g., Ruthenium tris(4,7-diphenyl-1,10-phenanthroline) dichloride) in a solvent such as dichloromethane. The mixture may be coated onto a structure such as a stainless steel sensor probe. In typical use, the inventive sensor probe is inserted into a flow cell through which the solution to be monitored flows. A blue LED light source having a wavelength of approximately 470 nm, and a spectrophotometer detector complete the sensor system. In operation, the fluorophore layer of the probe is illuminated by a blue LED light and the fluorescence is reflected back to the spectrophotometer. The monitored solution, typically an electroless gold plating bath, is pumped from the process equipment through the flow cell, and then back to the solution's origin.
摘要翻译：具有高达pH 14的溶液中可用和精确的氧传感器。通过将固定有与荧光团（例如三（4,7-二苯基-1,10-菲咯啉）钌的钌）的聚苯乙烯层固定在溶剂如二氯甲烷中来制造传感器。混合物可以涂覆到诸如不锈钢传感器探针的结构上。在典型的使用中，将本发明的传感器探针插入待监测的溶液流过的流动池中。具有约470nm波长的蓝色LED光源和分光光度计检测器完成传感器系统。在操作中，探针的荧光团层被蓝色LED光照射，荧光反射回分光光度计。监控的解决方案，通常是无电镀金浴，从工艺设备通过流动池泵送，然后回到溶液的起源。

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