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1. 发明申请

US20160281102A1 DNA Having Anther-Specific Promoter Activity, and Utilization Thereof 审中-公开
标题翻译：具有花药特异性启动子活性的DNA及其利用
公开(公告)号：US20160281102A1
公开(公告)日：2016-09-29
申请号：US14965947
申请日：2015-12-11
申请人： National Research & Development Agency National Agriculture & Food Research Organization , National Institute of Agrobiological Sciences
发明人： Junichi Tanaka , Yojiro Taniguchi , Maiko Akasaka , Yutaka Tabei , Kiyomi Abe , Masao Oshima
IPC分类号： C12N15/82
CPC分类号： C12N15/8231
摘要： A DNA having an anther-specific promoter activity, wherein the DNA is selected from the group consisting of the following (a) to (d): (a) a DNA containing a base sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 7; (b) a DNA containing a base sequence having a sequence identity of 85% or higher with a base sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 7; (c) a DNA containing a base sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 7 in which the base sequence undergoes at least one of substitution, deletion, insertion, and addition of one or several bases; and (d) a DNA containing a base sequence which hybridizes with a DNA consisting of a base sequence complementary to a base sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 7 under a stringent condition.
摘要翻译：一种具有花药特异性启动子活性的DNA，其中所述DNA选自以下（a）至（d）：（a）含有选自SEQ ID NO：1 至SEQ ID NO：7; （b）含有序列同一性为85％以上的碱基序列的DNA与选自SEQ ID NO：1至SEQ ID NO：7的碱基序列的DNA; （c）含有选自SEQ ID NO：1至SEQ ID NO：7的碱基序列的DNA，其中所述碱基序列经历至少一个取代，缺失，插入和添加一个或几个碱基; 和（d）含有碱基序列的DNA，所述碱基序列在严格条件下与由选自SEQ ID NO：1至SEQ ID NO：7的碱基序列互补的碱基序列组成的DNA杂交。

2. 发明授权

US09359416B2 DNA encoding canine monomeric CRP and expression vector containing the DNA 有权
标题翻译：编码犬单体CRP的DNA和含有DNA的表达载体
公开(公告)号：US09359416B2
公开(公告)日：2016-06-07
申请号：US14487513
申请日：2014-09-16
申请人： NITTO BOSEKI CO., LTD. , NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
发明人： Iwao Kiyokawa , Yuji Arimatsu , Toshihide Miura , Ryo Kojima , Hideki Sezutsu , Keiro Uchino , Isao Kobayashi , Toshiki Tamura
IPC分类号： C07K14/47 , A01K67/033 , A01K67/04
CPC分类号： C07K14/4737 , A01K67/0333 , A01K67/04 , A01K2217/052 , A01K2227/706 , C07K2319/02
摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.
摘要翻译：通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。

3. 发明申请

US20140319728A1 DRIED HYDROGEL, DRIED VITRIGEL MEMBRANE, AND METHODS FOR PRODUCING THE SAME 审中-公开
标题翻译：干燥水凝胶，干燥维生素膜及其制造方法
公开(公告)号：US20140319728A1
公开(公告)日：2014-10-30
申请号：US14326817
申请日：2014-07-09
申请人： Toshiaki Takezawa , NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES , KANTO KAGAKU KABUSHIKI KAISHA
发明人： Toshiaki TAKEZAWA , Ayumi Oshikata , Hiroyuki Kuroyama , Tomoya Sawaguchi , Hiroyuki Yamaguchi
IPC分类号： B29D7/01
CPC分类号： B29D7/01 , B29C39/02 , B29C41/02 , B29K2089/00 , C12M25/14 , C12N5/0062
摘要： A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.
摘要翻译：干燥玻璃凝胶膜是通过以下步骤制造的：（1）将水凝胶保持在壁表面模具内部的形状与设置在基板上的所需形状相同的形状的步骤，水凝胶内的自由水从基材和壁表面模具之间的间隙中释放出来; （2）从基板的顶部除去壁面模具的工序; （3）干燥水凝胶以去除残留游离水的步骤，由此制造玻璃化干燥水凝胶; （4）将干燥的水凝胶再水化以制造玻璃el膜的步骤; 和（5）重新搅拌玻璃浆膜以除去游离水的步骤，从而制造玻璃化的干燥玻璃凝胶膜。

4. 发明申请

US20140208458A1 Plant Having Complex Disease Resistance 有权
标题翻译：植物具有复杂的抗病性
公开(公告)号：US20140208458A1
公开(公告)日：2014-07-24
申请号：US13748334
申请日：2013-01-23
申请人： National Institute of Agrobiological Sciences
发明人： Hiroshi Takatsuji , Shingo Goto
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8239 , C12N15/8279 , C12N15/8281 , C12N15/8282 , Y02A40/146
摘要： The present invention relates to plants that have been transformed so as to have both disease resistance and acceptable agronomic traits. More specifically, the present invention relates to transgenic plants that have acquired disease resistance through expression in the plants of a polynucleotide encoding the transcription factor WRKY45 in an infection-responsive manner, and methods for generating the transgenic plants.
摘要翻译：本发明涉及已经转化以具有抗病性和可接受的农艺性状的植物。更具体地，本发明涉及通过以编码转录因子WRKY45的多核苷酸在植物中以感染反应的方式表达而获得抗病性的转基因植物以及用于产生转基因植物的方法。

5. 发明申请

US20040166578A1 Culture medium for in vitro culture of in vitro-produced porcine empryo and method for in vitro production of porcine embryo using the culture medium 失效
标题翻译：用于体外培养猪体外培养的培养基和使用培养基体外生产猪胚胎的方法
公开(公告)号：US20040166578A1
公开(公告)日：2004-08-26
申请号：US10790113
申请日：2004-03-02
申请人： National Institute of Agrobiological Sciences
发明人： Kazuhiro Kikuchi , Hiroyuki Kaneko , Junko Noguchi
IPC分类号： A01K067/027
CPC分类号： C12N5/0604 , C12N2500/30 , C12N2502/243
摘要： The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer. A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.
摘要翻译：本发明提供了用于体外培养的体外培养的猪胚胎的体外培养基，其可以改善所产生的囊胚的质量，并且可以提高转移后胎儿和婴儿的发育比例以及方法用于体外培养体外产生的使用培养基的猪胚胎，其可以改善所产生的囊胚的质量，并且可以在转移后将囊胚发育成胎儿和婴儿。用于体外培养体外培养的含有乳酸和丙酮酸的猪胚胎的培养基; 用于体外培养的培养基，其用输卵管上皮细胞调节; 以及体外培养体外产生的猪胚胎的方法，包括使用培养基培养体外产生的猪胚胎，在受精后0至2天，随后在含葡萄糖的培养基中培养胚胎。

6. 发明申请

US20030199684A1 Novel gene involved in brassinosteroid responses 审中-公开
标题翻译：涉及油菜素类固醇反应的新基因
公开(公告)号：US20030199684A1
公开(公告)日：2003-10-23
申请号：US10447135
申请日：2003-05-27
申请人： National Institute of Agrobiological Sciences
发明人： Hirohiko Hirochika , Muneo Yamazaki , Akio Miyao
IPC分类号： C07H021/04
CPC分类号： C12N15/8298 , C07K14/415
摘要： There is provided a polynucleotide encoding a plant gene capable of controlling a signal transduction system for brassinosteroid hormone, the polynucleotide encoding an amino acid sequence from Met at position 1 to Arg at position 1057 of SEQ ID NO: 2 in the SEQUENCE LISTING, including any polynucleotide encoding an amino acid sequence in which one or more amino acids are deleted, substituted or added to the amino acid sequence.
摘要翻译：提供了编码能够控制油菜素类固醇激素的信号转导系统的植物基因的多核苷酸，其编码序列表中SEQ ID NO：2的位置1处的Met从位置1的Met到第1077位的Arg的氨基酸序列，包括任何编码氨基酸序列的多核苷酸，其中一个或多个氨基酸被缺失，取代或添加到氨基酸序列中。

7. 发明授权

US11198264B2 Dried hydrogel, dried vitrigel membrane, and methods for producing the same 有权
公开(公告)号：US11198264B2
公开(公告)日：2021-12-14
申请号：US14326817
申请日：2014-07-09
申请人： NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES , Toshiaki Takezawa , KANTO KAGAKU KABUSHIKI KAISHA
发明人： Toshiaki Takezawa , Ayumi Oshikata , Hiroyuki Kuroyama , Tomoya Sawaguchi , Hiroyuki Yamaguchi
IPC分类号： C12M3/00 , B29D7/01 , B29C39/02 , B29C41/02 , C12N5/00 , C12M1/12
摘要： A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

8. 发明申请

US20170354129A1 BIOTINYLATED AND OXIDIZED LDL RECEPTOR AND ADVANCED GLYCATION END PRODUCT RECEPTOR PRODUCED USING GENETICALLY ENGINEERED SILKWORM 审中-公开
公开(公告)号：US20170354129A1
公开(公告)日：2017-12-14
申请号：US15515998
申请日：2015-10-01
申请人： National Agriculture and Food Research Organizatio , NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
发明人： Sachiko Machida , Miyuki Kuramochi , Mayumi Kameyama , Mari Yamamoto , Toshiro Kobori , Hideki Sezutsu , Ken-ichiro Tatematsu
IPC分类号： A01K67/027 , C07K16/00 , G01N33/92 , C07K14/47 , C12N9/00 , C07K14/705
CPC分类号： A01K67/0275 , A01K67/0339 , A01K2227/706 , A01K2267/01 , C07K14/4726 , C07K14/705 , C07K14/70503 , C07K16/00 , C07K16/005 , C07K16/18 , C07K2317/21 , C07K2317/622 , C07K2319/20 , C12N9/93 , C12Y603/04015 , G01N33/92 , G01N2333/705 , G01N2440/00 , G01N2800/323
摘要： The present invention pertains to a silkworm-type biotinylated CTLD14 or sRAGE and a method for manufacturing the same. One embodiment of the present invention provides a method for manufacturing biotinylated proteins, wherein the method includes A) a step for inserting a nucleic acid molecule for coding biotin ligase and protein in a coexpressable manner into a silkworm or a living organism that imparts sugar chains that are the same as the sugar chains of the silkworm, B) a step for causing the biotin ligase and protein to be expressed by disposing the silkworm or the living organism that imparts sugar chains that are the same as the sugar chains of the silkworm to conditions with which the nucleic acid molecule will carry out expression, and C) a step for administering biotin to the living organism and obtaining the biotinylated protein.

9. 发明申请

US20040060080A1 Gibberellin 2-oxidase gene, functions and uses thereof 失效
标题翻译：赤霉素2-氧化酶基因，其功能和应用
公开(公告)号：US20040060080A1
公开(公告)日：2004-03-25
申请号：US10392325
申请日：2003-03-18
申请人： National Institute of Agrobiological Sciences , Bio-Oriented Technology Research Advancement Institution
发明人： Hiroshi Tanaka , Toshiaki Kayano , Makoto Matsuoka , Masatomo Kobayashi , Tamio Saito , Tomoaki Sakamoto , Miho Sakai
IPC分类号： A01H001/00 , C12N015/82 , C12Q001/68 , C07H021/04 , C12N009/06 , C12N005/04
CPC分类号： C12N9/0071 , C07H21/04 , C12N15/8297
摘要： An objective of the present invention is to provide DNAs encoding novel plant proteins having a gibberellin (GA) 2-oxidation activity. Another objective is to modify plant height by utilizing these DNAs for regulating the gibberellin content. PCR was performed using degenerate primers, and novel OsGA2ox2 and OsGA2ox3 genes were obtained. The present inventors discovered that unlike products of other GA2-oxidases such as OsGA2ox1, the product of OsGA2ox3 catalyzes the two-step oxidation of GA20 to GA29 and then of GA29 to GA29-catabolite. In addition, the present inventors also found that the growth of the transgenic rice plants expressing OsGA2ox3 was suppressed as compared to control plants.
摘要翻译：本发明的目的是提供编码具有赤霉素（GA）2-氧化活性的新型植物蛋白质的DNA。另一个目标是通过利用这些DNA调节赤霉素含量来修饰植物高度。使用简并引物进行PCR，获得新的OsGA2ox2和OsGA2ox3基因。本发明人发现，与OsGA2ox1等其它GA2氧化酶的产物不同，OsGA2ox3的产物催化GA20至GA29的两步氧化，然后将GA29分解为GA29-分解代谢物。此外，本发明人还发现与对照植物相比，表达OsGA2ox3的转基因水稻植物的生长被抑制。

10. 发明申请

US20030059935A1 Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of procine embryo using the culture medium 失效
标题翻译：用于体外培养的体外生产的猪胚胎的培养基和使用培养基体外生产原核胚的方法
公开(公告)号：US20030059935A1
公开(公告)日：2003-03-27
申请号：US10061321
申请日：2002-02-04
申请人： National Institute of Agrobiological Sciences
发明人： Kazuhiro Kikuchi , Hiroyuki Kaneko , Junko Noguchi
IPC分类号： C12N015/00 , C12N005/06
CPC分类号： C12N5/0604 , C12N2500/30 , C12N2502/243
摘要： The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer. A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.
摘要翻译：本发明提供了用于体外培养的体外培养的猪胚胎的体外培养基，其可以改善所产生的囊胚的质量，并且可以提高转移后胎儿和婴儿的发育比例以及方法用于体外培养体外产生的使用培养基的猪胚胎，其可以改善所产生的囊胚的质量，并且可以在转移后将囊胚发育成胎儿和婴儿。用于体外培养体外培养的含有乳酸和丙酮酸的猪胚胎的培养基; 用于体外培养的培养基，其用输卵管上皮细胞调节; 以及体外培养体外产生的猪胚胎的方法，包括使用培养基培养体外产生的猪胚胎，在施肥后0至2天，随后在含葡萄糖的培养基中培养胚胎。

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