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1. 发明授权

US08921077B2 Method of producing a diutan gum 有权
标题翻译：生产二丹胶的方法
公开(公告)号：US08921077B2
公开(公告)日：2014-12-30
申请号：US13599202
申请日：2012-08-30
申请人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
发明人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
IPC分类号： C12P19/04 , A61K9/48 , A61K47/38 , C08B37/00 , A61K9/70
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译：与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

2. 发明授权

US08709782B2 Targeted gene deletions for polysaccharide slime formers 有权
标题翻译：多糖粘液形成物的靶向基因缺失
公开(公告)号：US08709782B2
公开(公告)日：2014-04-29
申请号：US12983935
申请日：2011-01-04
申请人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
发明人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
IPC分类号： C12N1/20 , C12P19/04 , C12Q1/68 , C12N15/74 , C07H21/04
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene N Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译：本发明提供由突变基因M或基因N鞘氨醇单胞菌菌株产生的改善的多糖（例如，结冷糖和二萜），其含有至少一种有利于形成泥质多糖的生物的遗传修饰。还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。

3. 发明申请

US20110281334A1 TARGETED GENE DELETIONS FOR POLYSACCHARIDE SLIME FORMERS 有权
标题翻译：针对多糖制剂的目标基因删除
公开(公告)号：US20110281334A1
公开(公告)日：2011-11-17
申请号：US12983935
申请日：2011-01-04
申请人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
发明人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
IPC分类号： C12N1/21
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene M or gene N Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译：本发明提供由突变基因M或基因N鞘氨醇单胞菌菌株产生的改善的多糖（例如，结冷糖和二萜），其含有至少一种有利于形成泥质多糖的生物的遗传修饰。还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。

4. 发明申请

US20080268527A1 Mutant bacterial strains of the genus sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 有权
标题翻译：突变型疏水螺旋体属细菌菌株缺乏生产聚羟基丁酸酯和一种澄清鞘氨醇及其组合物的方法
公开(公告)号：US20080268527A1
公开(公告)日：2008-10-30
申请号：US11292356
申请日：2005-12-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： C08B37/00
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

5. 发明申请

US20130084607A1 Method of Producing a Diutan Gum 有权
标题翻译：生产杜鹃胶的方法
公开(公告)号：US20130084607A1
公开(公告)日：2013-04-04
申请号：US13599202
申请日：2012-08-30
申请人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
发明人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
IPC分类号： C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad host range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译：与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽的宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

6. 发明授权

US07868167B2 High viscosity diutan gums 有权
标题翻译：高粘度二萜胶
公开(公告)号：US07868167B2
公开(公告)日：2011-01-11
申请号：US11264268
申请日：2005-11-01
申请人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
发明人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
IPC分类号： C08B37/00 , C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译：与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

7. 发明授权

US07361754B2 Genetically purified gellan gum 有权
标题翻译：基因纯化的结冷胶
公开(公告)号：US07361754B2
公开(公告)日：2008-04-22
申请号：US11156953
申请日：2005-06-20
申请人： Joseph M. Cleary , Russell J. Coleman , Nancy E. Harding , Yamini N. Patel
发明人： Joseph M. Cleary , Russell J. Coleman , Nancy E. Harding , Yamini N. Patel
IPC分类号： C07G17/00 , C12N1/20 , C12N15/87 , C09K3/00 , A23C9/154 , A23C9/12
CPC分类号： C08L5/00 , A23C9/137 , A23C9/1544 , A23L29/272 , C08B37/006 , C12N9/16 , C12N9/2402 , C12N9/2405 , C12P19/04 , C12Y302/01031
摘要： Mutational inactivation of proteins involved in para-cresol production in certain milk products results in improved taste and odor. The undesirable para-cresol forms over time as a result of enzymes produced by the bacterium that produces gellan gum. Since the gellan is typically used in a relatively unpurified form, the enzymes are added to the milk along with the gellan. Inactivation of the enzymes is a genetic means of eliminating the enzymes without requiring any additional purification or processing.
摘要翻译：涉及某些乳制品中对甲酚生产的蛋白质的突变失活导致改善的味道和气味。作为由产生结冷胶的细菌产生的酶的结果，不期望的对甲酚随时间而形成。由于结冷胶通常以相对未纯化的形式使用，所以将酶与吉兰糖一起加入到牛奶中。酶的失活是消除酶的遗传手段，而不需要任何额外的纯化或加工。

8. 发明授权

US08759071B2 Targeted gene deletions for polysaccharide slime formers 有权
标题翻译：多糖粘液形成物的靶向基因缺失
公开(公告)号：US08759071B2
公开(公告)日：2014-06-24
申请号：US12983922
申请日：2011-01-04
申请人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
发明人： Nancy E. Harding , Yamini N. Patel , Russell J. Coleman
IPC分类号： C12N15/74
CPC分类号： C12N15/74 , C07K14/195 , C12N15/52 , C12P19/04
摘要： The present invention provides improved polysaccharides (e.g., gellan and diutan) produced by mutant gene I Sphingomonas strains containing at least one genetic modification that favors slime-forming polysaccaride production. Methods of making the mutant Sphingomonas strains and the culture broth containing such mutant Sphingomonas strains are also provided.
摘要翻译：本发明提供了由含有至少一种有利于形成泥质多糖生产的遗传修饰的突变基因I鞘氨醇单胞菌菌株产生的改良多糖（例如，结冷糖和二萜）。还提供了制备突变型鞘氨醇单胞菌菌株和含有这种突变型鞘氨醇单胞菌属菌株的培养液的方法。

9. 发明授权

US08652792B1 Mutant bacterial strains of the genus Sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 失效
标题翻译：缺乏生产聚羟基丁酸酯的鞘氨醇单胞菌属的突变细菌菌株和鞘氨醇及其组合物的澄清方法
公开(公告)号：US08652792B1
公开(公告)日：2014-02-18
申请号：US12916828
申请日：2010-11-01
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： G01N33/554
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PUB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属的突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变菌株产生PHB缺乏的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括将鞘氨醇水溶液，特别是PHB缺乏的鞘氨醇溶液加热至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺乏和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

10. 发明授权

US08278438B2 High viscosity diutan gums 有权
标题翻译：高粘度二萜胶
公开(公告)号：US08278438B2
公开(公告)日：2012-10-02
申请号：US12892561
申请日：2010-09-28
申请人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
发明人： Nancy E. Harding , Yamini N. Patel , Russell Coleman , Steven Matzke
IPC分类号： C08B37/00 , C12P19/04
CPC分类号： C12N1/20 , A61K9/4816 , A61K9/7007 , A61K47/38 , C08B11/12 , C08B37/0033 , C08L1/286 , C12N9/10 , C12P19/04 , C12P19/06
摘要： The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of Sphingomonas sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host Sphingomonas strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention. Additionally, the novel engineered Sphingomonas strain including the needed DNA sequence is encompassed within this invention.
摘要翻译：与以前生产的相同类型的重复单元的多糖相比，生产具有增加的粘度特性的二脱水多糖。这种改进的二脱水多糖通过产生鞘氨醇单胞菌属的衍生物而产生。 ATCC 53159含有多拷贝宽宿主范围质粒，其中已经克隆了用于生物合成二an多糖的基因。质粒提供宿主鞘氨醇单胞菌菌株内的能力，以产生这种多糖合成的多个基因拷贝。以这种方式，提供了不仅增加目标二脱水多糖的生产的方法，而且提供了具有改善的物理性质（上述较高粘度）的二脱水多糖的方法。这种二an多糖被证明在油田应用中和水泥材料中作为可能的增粘剂是特别有用的。本发明还包括生产这种改进的二脱氧多糖的本发明方法，以及在这种方法中生产改进的二萜所需的新的克隆基因。此外，包含所需DNA序列的新型工程化鞘氨醇单胞菌菌株包括在本发明中。

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