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1. 发明授权

US08652792B1 Mutant bacterial strains of the genus Sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 失效
标题翻译：缺乏生产聚羟基丁酸酯的鞘氨醇单胞菌属的突变细菌菌株和鞘氨醇及其组合物的澄清方法
公开(公告)号：US08652792B1
公开(公告)日：2014-02-18
申请号：US12916828
申请日：2010-11-01
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： G01N33/554
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PUB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属的突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变菌株产生PHB缺乏的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括将鞘氨醇水溶液，特别是PHB缺乏的鞘氨醇溶液加热至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺乏和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

2. 发明授权

US07887866B2 Clarification of sphingans and compositions thereof 有权
标题翻译：澄清鞘氨醇及其组合物
公开(公告)号：US07887866B2
公开(公告)日：2011-02-15
申请号：US09798642
申请日：2001-03-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： A23L1/054
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

3. 发明申请

US20110009611A1 CLARIFICATION OF SPHINGANS AND COMPOSITIONS THEREOF 有权
标题翻译：菠菜及其组合物的澄清
公开(公告)号：US20110009611A1
公开(公告)日：2011-01-13
申请号：US09798642
申请日：2001-03-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： C08B37/00
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

4. 发明授权

US07829697B2 Mutant bacterial strains of the genus Sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 有权
标题翻译：缺乏生产聚羟基丁酸酯的鞘氨醇单胞菌属的突变细菌菌株和鞘氨醇及其组合物的澄清方法
公开(公告)号：US07829697B2
公开(公告)日：2010-11-09
申请号：US11292356
申请日：2005-12-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： C08B37/00 , C07G17/00
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

5. 发明授权

US08017355B2 Mannitol induced promoter systems in bacterial host cells 有权
标题翻译：甘露醇诱导细菌宿主细胞中的启动子系统
公开(公告)号：US08017355B2
公开(公告)日：2011-09-13
申请号：US12330723
申请日：2008-12-09
申请人： J. Carrie Schneider , Bettina Rosner
发明人： J. Carrie Schneider , Bettina Rosner
IPC分类号： C12P21/00 , C12N1/21 , C07H21/04
CPC分类号： C07K14/21 , C12N15/78 , C12P21/02
摘要： The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.
摘要翻译：本发明提供了利用甘露醇，阿糖醇，葡萄糖醇或甘油诱导型启动子在细菌宿主中产生重组肽的方法，其中产生该肽的宿主细菌细胞已经不能降解或代谢甘露醇，阿糖醇或葡萄糖醇，或其衍生物或类似物。本发明提供已被遗传改变以抑制甘露醇，葡萄糖醇或阿拉伯糖醇或其衍生物或类似物的代谢或降解的细菌细胞。本发明利用甘露醇，阿糖醇，葡萄糖醇或甘油从诱导型启动子诱导靶多肽的表达，允许在用于生产重组肽的发酵过程中使用便宜且稳定的碳源诱导剂。

6. 发明申请

US20090162899A1 MANNITOL INDUCED PROMOTER SYSTEMS IN BACTERIAL HOST CELLS 有权
标题翻译： MANNITOL诱导的细菌系统在细菌宿主细胞
公开(公告)号：US20090162899A1
公开(公告)日：2009-06-25
申请号：US12330723
申请日：2008-12-09
申请人： J. Carrie Schneider , Bettina Rosner
发明人： J. Carrie Schneider , Bettina Rosner
IPC分类号： C12P21/00
CPC分类号： C07K14/21 , C12N15/78 , C12P21/02
摘要： The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.
摘要翻译：本发明提供了利用甘露醇，阿糖醇，葡萄糖醇或甘油诱导型启动子在细菌宿主中产生重组肽的方法，其中产生该肽的宿主细菌细胞已经不能降解或代谢甘露醇，阿糖醇或葡萄糖醇，或其衍生物或类似物。本发明提供已被遗传改变以抑制甘露醇，葡萄糖醇或阿拉伯糖醇或其衍生物或类似物的代谢或降解的细菌细胞。本发明利用甘露醇，阿糖醇，葡萄糖醇或甘油从诱导型启动子诱导靶多肽的表达，允许在用于生产重组肽的发酵过程中使用便宜且稳定的碳源诱导剂。

7. 发明授权

US07476532B2 Mannitol induced promoter systems in bacterial host cells 有权
标题翻译：甘露醇诱导细菌宿主细胞中的启动子系统
公开(公告)号：US07476532B2
公开(公告)日：2009-01-13
申请号：US11447553
申请日：2006-06-06
申请人： J. Carrie Schneider , Bettina Rosner
发明人： J. Carrie Schneider , Bettina Rosner
IPC分类号： C12N1/21 , C12N15/09 , C07H21/04
CPC分类号： C07K14/21 , C12N15/78 , C12P21/02
摘要： The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.
摘要翻译：本发明提供了利用甘露醇，阿糖醇，葡萄糖醇或甘油诱导型启动子在细菌宿主中产生重组肽的方法，其中产生该肽的宿主细菌细胞已经不能降解或代谢甘露醇，阿糖醇或葡萄糖醇，或其衍生物或类似物。本发明提供已被遗传改变以抑制甘露醇，葡萄糖醇或阿拉伯糖醇或其衍生物或类似物的代谢或降解的细菌细胞。本发明利用甘露醇，阿糖醇，葡萄糖醇或甘油从诱导型启动子诱导靶多肽的表达，允许在用于生产重组肽的发酵过程中使用便宜且稳定的碳源诱导剂。

8. 发明申请

US20080268527A1 Mutant bacterial strains of the genus sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 有权
标题翻译：突变型疏水螺旋体属细菌菌株缺乏生产聚羟基丁酸酯和一种澄清鞘氨醇及其组合物的方法
公开(公告)号：US20080268527A1
公开(公告)日：2008-10-30
申请号：US11292356
申请日：2005-12-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： C08B37/00
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

9. 发明授权

US08865241B1 Mutant bacterial strains of the genus Sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 有权
标题翻译：缺乏生产聚羟基丁酸酯的鞘氨醇单胞菌属的突变细菌菌株和鞘氨醇及其组合物的澄清方法
公开(公告)号：US08865241B1
公开(公告)日：2014-10-21
申请号：US12893322
申请日：2010-09-29
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： A23L1/05 , C07H17/00 , C08B37/00 , C07H1/06 , C07H1/08
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient Sphingans. The invention is also directed to a process for preparing a clarified Sphingan solution comprising heating aqueous Sphingan solution, in particular PHB-deficient Sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified Sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属的突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变菌株产生PHB缺乏的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括将鞘氨醇水溶液，特别是PHB缺乏的鞘氨醇溶液加热至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺乏和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

10. 发明授权

US08198064B2 Mutant bacterial strains of the genus sphingomonas deficient in production of polyhydroxybutyrate and a process of clarification of sphingans and compositions thereof 有权
标题翻译：突变型疏水螺旋体属细菌菌株缺乏生产聚羟基丁酸酯和澄清的鞘氨醇及其组合物的方法
公开(公告)号：US08198064B2
公开(公告)日：2012-06-12
申请号：US11292366
申请日：2005-12-02
申请人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
发明人： Stan Bower , Ellen Burke , Nancy E. Harding , Yamini N. Patel , J. Carrie Schneider , Dagmar Meissner , Neil A. Morrison , Ralph Bezanson
IPC分类号： C12N1/12 , C12N15/00 , C12N9/10 , C07H21/02 , C07H21/04
CPC分类号： C08B37/006 , A23L21/10 , A23L21/15 , A23L29/269 , C12N1/20 , C12P7/62 , C12P19/04
摘要： The invention relates to mutant strains of the genus Sphingomonas which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30° C. to about 70° C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.
摘要翻译：本发明涉及鞘氨醇单胞菌属突变菌株，其在编码参与多羟基丁酸酯（“PHB”）合成的蛋白质的至少一个基因中具有突变，其允许突变株产生PHB缺陷的鞘氨醇。本发明还涉及一种制备澄清的鞘氨醇溶液的方法，其包括加热水性鞘氨醇溶液，特别是PHB缺陷的鞘氨醇溶液至约30℃至约70℃的澄清温度，并用澄清剂和酶。此外，本发明涉及包含PHB缺陷和/或澄清的鞘氨醇的食品或工业产品。本发明的一个具体实施方案涉及澄清的PHB缺陷型高酰基结冷胶及其制备方法。

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