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1. 发明授权

US06759231B2 Phage with nuclear localization signal 失效
标题翻译：噬菌体与核定位信号
公开(公告)号：US06759231B2
公开(公告)日：2004-07-06
申请号：US09844813
申请日：2001-04-27
申请人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
发明人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
IPC分类号： C12N120
CPC分类号： C12N15/1037 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2795/10343
摘要： A &lgr; phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a &lgr; phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant &lgr; phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting &lgr; phage is capable of packaging &lgr; phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.
摘要翻译：已经通过构建能够在构成λ噬菌体的头部的gpD蛋白质和核定位信号序列之间表达融合蛋白质的载体，用该载体转化大肠杆菌并扩增突变体来获得具有核定位信号的λ噬菌体 λ噬菌体在该转化体中不能在大肠杆菌中表达gpD蛋白。已经证实所得的λ噬菌体能够包装80％和100％基因组大小的λ噬菌体DNA。在进一步证实核定位信号暴露在该噬菌体头部的外部时，该噬菌体已被微量注入细胞以分析其核定位活性。因此，已经澄清这种噬菌体具有核定位活动。

2. 发明授权

US08496941B2 Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same 有权
标题翻译：用于产生多能干细胞的载体和使用其制备多能干细胞的方法
公开(公告)号：US08496941B2
公开(公告)日：2013-07-30
申请号：US12792580
申请日：2010-06-02
申请人： Mahito Nakanishi , Ken Nishimura , Manami Ohtaka , Masayuki Sano
发明人： Mahito Nakanishi , Ken Nishimura , Manami Ohtaka , Masayuki Sano
IPC分类号： A61K39/12 , A61K39/155 , C12N5/00 , C12N15/00 , C12N15/86
CPC分类号： C12N5/0696 , C12N15/1131 , C12N15/86 , C12N15/8775 , C12N15/8776 , C12N2310/14 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18843
摘要： Stem cell reprogramming genes cloned into a single sustained expression-type Sendai viral vector are shown to reprogram differentiated somatic cells into induced pluripotent stem (iPS) cells without integration of vector sequences into the host cell's genome. The genes are transduced into normal differentiated somatic cells via infection with recombinant Sendai virus. After expression of the reprogramming genes and subsequent induction of pluripotency, the vector genome RNA including the reprogramming genes is removed from the cell to establish an iPS cell that is genetically identical to the parent somatic differentiated cell thus reducing the risk of tumorigenic transformation caused by random integration of vector sequences into the host genome. The method promises to provide safe, autologous iPS cells that can be used for human cell replacement and regeneration therapeutic applications.
摘要翻译：克隆到单一持续表达型仙台病毒载体中的干细胞重编程基因显示将分化的体细胞重编程为诱导多能干细胞（iPS）细胞，而不将载体序列整合到宿主细胞的基因组中。通过感染重组仙台病毒将基因转导入正常分化的体细胞。在重编程基因表达和随后的多能性诱导之后，从细胞中除去包括重编程基因的载体基因组RNA以建立与亲本体细胞分化细胞遗传相同的iPS细胞，从而降低由随机引起的致瘤转化的风险将载体序列整合到宿主基因组中。该方法有望提供可用于人类细胞替代和再生治疗应用的安全的自体iPS细胞。

3. 发明授权

US06235521B1 Phage bonded to a nuclear location signal 失效
标题翻译：噬菌体与核定位信号
公开(公告)号：US06235521B1
公开(公告)日：2001-05-22
申请号：US09242131
申请日：1999-09-10
申请人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
发明人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
IPC分类号： C12N700
CPC分类号： C12N15/1037 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2795/10343
摘要： A &lgr; phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a &lgr; phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant &lgr; phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting &lgr; phage is capable of packaging &lgr; phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.
摘要翻译：通过构建能够在构成羔羊噬菌体的头部的gpD蛋白质和核定位信号序列之间表达融合蛋白的载体，通过该载体转化大肠杆菌并扩增突变体，获得具有核定位信号的羔羊噬菌体在该转化体中不能在大肠杆菌中表达gpD蛋白的羔羊噬菌体。已经证实，所得的羔羊噬菌体能够包装80％和100％基因组大小的羔羊噬菌体DNA。在进一步证实核定位信号暴露在该噬菌体头部的外部时，该噬菌体已被微量注入细胞以分析其核定位活性。因此，已经澄清这种噬菌体具有核定位活动。

4. 发明申请

US20100196993A1 PERSISTENTLY INFECTIVE SENDAI VIRUS VECTOR 有权
标题翻译：不寻常的感染性SENDAI病毒载体
公开(公告)号：US20100196993A1
公开(公告)日：2010-08-05
申请号：US12595497
申请日：2008-04-11
申请人： Ken Nishimura , Hiroaki Segawa , Mahito Nakanishi
发明人： Ken Nishimura , Hiroaki Segawa , Mahito Nakanishi
IPC分类号： C12N7/04 , C07H21/04 , C12N15/63
CPC分类号： C12N15/86 , A61K35/12 , A61K35/13 , A61K38/162 , C12N7/00 , C12N2760/18821 , C12N2760/18843
摘要： A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.
摘要翻译：通过使用如此修饰的基因来编码持续感染性病毒载体，以编码氨基酸序列，所述氨基酸序列包括取代氨基酸序列中位置1618处的氨基酸残基的氨基酸序列，用于持续非感染性仙台病毒的L蛋白。通过缺失或缺失M基因，F基因和HN基因中的至少一个也产生不可传播的持续感染性病毒载体。这些病毒载体没有细胞毒性，可以长时间达到持续的基因表达，是安全的，因此是有用的。

5. 发明授权

US06300120B1 Phage with nuclear localization signal 失效
公开(公告)号：US06300120B1
公开(公告)日：2001-10-09
申请号：US09615283
申请日：2000-07-13
申请人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
发明人： Mahito Nakanishi , Emi Nagoshi , Teruo Akuta , Katsuo Takeda , Mamoru Hasegawa
IPC分类号： C07N2104
CPC分类号： C12N15/1037 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2795/10343
摘要： A &lgr; phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a &lgr; phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant &lgr; phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting &lgr; phage is capable of packaging &lgr; phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.

6. 发明申请

US20120214240A1 VECTORS FOR GENERATING PLURIPOTENT STEM CELLS AND METHODS OF PRODUCING PLURIPOTENT STEM CELLS USING THE SAME 有权
标题翻译：用于产生多发性干细胞的方法和使用该方法生产细胞干细胞的方法
公开(公告)号：US20120214240A1
公开(公告)日：2012-08-23
申请号：US13292953
申请日：2011-11-09
申请人： Mahito NAKANISHI , Ken NISHIMURA , Masayuki SANO , Manami OHTAKA
发明人： Mahito NAKANISHI , Ken NISHIMURA , Masayuki SANO , Manami OHTAKA
IPC分类号： C12N15/86 , C12N5/0789 , C07H21/02 , C12N7/01
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/1131 , C12N15/8775 , C12N15/8776 , C12N2310/14 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18843
摘要： A reprogramming gene-loaded Sendai viral vector comprising Sendai virus genes and reprogramming genes, wherein the Sendai virus genes include an NP gene, P/C gene, M gene, F gene, HN gene and L gene, wherein each of the M gene, the F gene and the FIN gene is from a Sendai virus strain Cl.151-derived gene and wherein at least one of the M gene, the F gene and the HN gene is functionally deleted and the L gene encodes the amino-acid sequence of the L protein in which the amino-acid residue at position 1618 is valine and a method of producing the same.
摘要翻译：包含仙台病毒基因和重编程基因的重编程基因载体仙台病毒载体，其中仙台病毒基因包括NP基因，P / C基因，M基因，F基因，HN基因和L基因，其中M基因， F基因和FIN基因来自仙台病毒株Cl.151衍生的基因，其中M基因，F基因和HN基因中的至少一个在功能上被缺失，并且L基因编码氨基酸序列 1618位氨基酸残基为缬氨酸的L蛋白及其制备方法。

7. 发明申请

US20100311171A1 VECTORS FOR GENERATING PLURIPOTENT STEM CELLS AND METHODS OF PRODUCING PLURIPOTENT STEM CELLS USING THE SAME 有权
标题翻译：用于产生多发性干细胞的方法和使用该方法生产细胞干细胞的方法
公开(公告)号：US20100311171A1
公开(公告)日：2010-12-09
申请号：US12792580
申请日：2010-06-02
申请人： Mahito NAKANISHI , Ken NISHIMURA , Manami OHTAKA , Masayuki SANO
发明人： Mahito NAKANISHI , Ken NISHIMURA , Manami OHTAKA , Masayuki SANO
IPC分类号： C12N15/86 , C12N15/63 , C12N5/10 , C07H21/02 , C12N7/01
CPC分类号： C12N5/0696 , C12N15/1131 , C12N15/86 , C12N15/8775 , C12N15/8776 , C12N2310/14 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18843
摘要： Stem cell reprogramming genes cloned into a single sustained expression-type Sendai viral vector are shown to reprogram differentiated somatic cells into induced pluripotent stem (iPS) cells without integration of vector sequences into the host cell's genome. The genes are transduced into normal differentiated somatic cells via infection with recombinant Sendai virus. After expression of the reprogramming genes and subsequent induction of pluripotency, the vector genome RNA including the reprogramming genes is removed from the cell to establish an iPS cell that is genetically identical to the parent somatic differentiated cell thus reducing the risk of tumorigenic transformation caused by random integration of vector sequences into the host genome. The method promises to provide safe, autologous iPS cells that can be used for human cell replacement and regeneration therapeutic applications.
摘要翻译：克隆到单一持续表达型仙台病毒载体中的干细胞重编程基因显示将分化的体细胞重编程为诱导多能干细胞（iPS）细胞，而不将载体序列整合到宿主细胞的基因组中。通过感染重组仙台病毒将基因转导入正常分化的体细胞。在重编程基因表达和随后的多能性诱导之后，从细胞中除去包括重编程基因的载体基因组RNA以建立与亲本体细胞分化细胞遗传相同的iPS细胞，从而降低由随机引起的致瘤转化的风险将载体序列整合到宿主基因组中。该方法有望提供可用于人类细胞替代和再生治疗应用的安全的自体iPS细胞。

8. 发明授权

US06740524B1 Nucleic acid transfer phage 失效
标题翻译：核酸转运噬菌体
公开(公告)号：US06740524B1
公开(公告)日：2004-05-25
申请号：US09720003
申请日：2001-09-04
申请人： Teruo Akuta , Haruhiko Yokoi , Hajime Okuyama , Katsuo Takeda , Mamoru Hasegawa , Mahito Nakanishi
发明人： Teruo Akuta , Haruhiko Yokoi , Hajime Okuyama , Katsuo Takeda , Mamoru Hasegawa , Mahito Nakanishi
IPC分类号： C12N1586
CPC分类号： C07K14/005 , A61K48/00 , C07K2319/01 , C07K2319/09 , C12N15/86 , C12N2740/16322 , C12N2800/30 , Y10S435/975
摘要： The present invention provides a novel phage expressing in its head a bi-functional protein that has nuclear translocation and cell adhesion activities. The phage is used to package a foreign substance such as a gene. As a bi-functional protein, TAT protein of HIV can be used. The phage is useful in gene therapy.
摘要翻译：本发明提供了一种在其头部表达具有核易位和细胞粘附活性的双功能蛋白质的新型噬菌体。噬菌体用于包装诸如基因的异物。作为双功能蛋白质，可以使用HIV的TAT蛋白。噬菌体可用于基因治疗。

9. 发明授权

US09365866B2 Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same 有权
标题翻译：用于产生多能干细胞的载体和使用其制备多能干细胞的方法
公开(公告)号：US09365866B2
公开(公告)日：2016-06-14
申请号：US13292953
申请日：2011-11-09
申请人： Mahito Nakanishi , Ken Nishimura , Masayuki Sano , Manami Ohtaka
发明人： Mahito Nakanishi , Ken Nishimura , Masayuki Sano , Manami Ohtaka
IPC分类号： C12N15/63 , C12N7/01 , C12N15/864 , C12N5/0789 , C12N15/86 , C12N5/074 , C12N15/113 , C12N15/877
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/1131 , C12N15/8775 , C12N15/8776 , C12N2310/14 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2760/18843
摘要： A reprogramming gene-loaded Sendai viral vector comprising Sendai virus genes and reprogramming genes, wherein the Sendai virus genes include an NP gene, P/C gene, M gene, F gene, HN gene and L gene, wherein each of the M gene, the F gene and the FIN gene is from a Sendai virus strain Cl.151-derived gene and wherein at least one of the M gene, the F gene and the HN gene is functionally deleted and the L gene encodes the amino-acid sequence of the L protein in which the amino-acid residue at position 1618 is valine and a method of producing the same.
摘要翻译：包含仙台病毒基因和重编程基因的重编程基因载体仙台病毒载体，其中仙台病毒基因包括NP基因，P / C基因，M基因，F基因，HN基因和L基因，其中M基因， F基因和FIN基因来自仙台病毒株Cl.151衍生的基因，其中M基因，F基因和HN基因中的至少一个在功能上被缺失，并且L基因编码氨基酸序列 1618位氨基酸残基为缬氨酸的L蛋白及其制备方法。

10. 发明授权

US09145564B2 Persistently infective Sendai virus vector 有权
标题翻译：持续感染仙台病毒载体
公开(公告)号：US09145564B2
公开(公告)日：2015-09-29
申请号：US12595497
申请日：2008-04-11
申请人： Ken Nishimura , Hiroaki Segawa , Mahito Nakanishi
发明人： Ken Nishimura , Hiroaki Segawa , Mahito Nakanishi
IPC分类号： C12N15/00 , C07H21/02 , C12N7/01 , A61K39/12 , A61K39/155 , A01N63/00 , C12N15/86 , A61K38/16 , C12N7/00 , A61K35/12 , A61K35/13
CPC分类号： C12N15/86 , A61K35/12 , A61K35/13 , A61K38/162 , C12N7/00 , C12N2760/18821 , C12N2760/18843
摘要： A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.
摘要翻译：通过使用如此修饰的基因来编码持续感染性病毒载体，以编码氨基酸序列，所述氨基酸序列包括取代氨基酸序列中位置1618处的氨基酸残基的氨基酸序列，用于持续非感染性仙台病毒的L蛋白。通过缺失或缺失M基因，F基因和HN基因中的至少一个也产生不可传播的持续感染性病毒载体。这些病毒载体没有细胞毒性，可以长时间达到持续的基因表达，是安全的，因此是有用的。

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