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    • 8. 发明授权
    • Process for preparing liposome composition
    • 制备脂质体组合物的方法
    • US5662931A
    • 1997-09-02
    • US505180
    • 1995-08-11
    • Koji MunechikaTomoyo SekiNorihide KishiHiroshi MatsudaYasuo Ueda
    • Koji MunechikaTomoyo SekiNorihide KishiHiroshi MatsudaYasuo Ueda
    • A61K9/127
    • A61K9/1277
    • The object of the present invention is to provide a process for preparing a drug-containing liposome composition in which (1) a drug to be included into liposomes, particularly a physiologically active protein having a molecular weight of from 500 to 100,000, can be prevented from decomposition and (2) a high rate of drug inclusion can be attained; (3) the resulting liposome composition can be subcutaneously or intramuscularly administered and (4) makes contribution to sustained release of the drug. The process comprises (1) dissolving a lipid in a first organic solvent, (2) adding a drug-containing aqueous solution to the lipid solution, followed by emulsifying to obtain an emulsion, (3) mixing the emulsion at a low temperature with a second organic solvent in which the lipid is sparingly soluble, (4) collecting the precipitated fraction, and (5) suspending the precipitated fraction in an aqueous solvent.
    • PCT No.PCT / JP94 / 00075 Sec。 371日期:1995年8月11日 102(e)日期1995年8月11日PCT 1994年1月20日PCT PCT。 WO94 / 18948 PCT公开 日本1994年9月1日本发明的目的是提供一种制备含药物的脂质体组合物的方法,其中(1)包含在脂质体中的药物,特别是分子量为500〜 100,000,可以防止分解,(2)可以获得高速率的药物包合; (3)所得的脂质体组合物可以皮下或肌肉内施用,(4)有助于药物的持续释放。 该方法包括(1)将脂质溶解在第一有机溶剂中,(2)向脂质溶液中加入含药水溶液,然后乳化以获得乳液,(3)将低温下的乳液与 第二有机溶剂,其中脂质微溶,(4)收集沉淀的级分,和(5)将沉淀的级分悬浮在水性溶剂中。