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1. 发明授权

US06689614B1 Method of identifying and treating invasive carcinomas 失效
标题翻译：识别和治疗侵袭性癌的方法
公开(公告)号：US06689614B1
公开(公告)日：2004-02-10
申请号：US10369032
申请日：2003-02-18
申请人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
发明人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
IPC分类号： G01N3350
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158
摘要： Prostasin protein has been found to be a useful marker for determination of the invasiveness of and as a means to treat human carcinomas. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in normal human prostate epithelial cells and the human prostate cancer cell line LNCaP, but not in the highly invasive human prostate cancer cell lines DU-145 and PC-3. Imunohistochemistry studies of human prostate cancer specimens revealed a down-regulation of prostasin in high-grade tumors. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in a non-invasive human breast cancer cell line, MCF-7, while invasive human breast cancer cell lines MDA-MB-231 and MDA-MB-435s were found not to express either the prostasin protein or the mRNA. A non-invasive human breast cancer cell line, MDA-MB-453, was shown to express prostasin mRNA but not prostasin protein. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68% and 42%, respectively. Transfection of MDA-MB-231 and MDA-MB-435s cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 50% for either cell line.
摘要翻译：已经发现前列腺素蛋白是用于测定人类癌的侵袭性和作为手段治疗人类癌症的有用标志物。使用RT-PCR和蛋白质印迹分析，在正常人前列腺上皮细胞和人前列腺癌细胞系LNCaP中发现前列腺素蛋白和mRNA表达，但不存在于高侵袭性人前列腺癌细胞系DU-145和PC-3中。人类前列腺癌标本的免疫组织化学研究显示高级肿瘤中前列腺素的下调。使用RT-PCR和蛋白质印迹分析，在非侵入性人乳腺癌细胞系MCF-7中发现前列腺素蛋白和mRNA表达，而侵袭性人乳腺癌细胞系MDA-MB-231和MDA-MB-435s分别为发现不表达前列腺素蛋白或mRNA。非侵入性人乳腺癌细胞系MDA-MB-453显示表达前列腺素mRNA而不是前列腺素蛋白。 DU-145和PC-3细胞用全长人前列腺素cDNA的转染能够恢复前列腺素的表达，体外侵袭能力分别降低了68％和42％。用全长人前列腺素cDNA转染MDA-MB-231和MDA-MB-435s细胞恢复前列腺素的表达，并将任何细胞系的体外侵袭力降低50％。

2. 发明授权

US06706483B1 Method of identifying and treating invasive carcinomas 失效
标题翻译：识别和治疗侵袭性癌的方法
公开(公告)号：US06706483B1
公开(公告)日：2004-03-16
申请号：US10368750
申请日：2003-02-18
申请人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
发明人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
IPC分类号： C12Q168
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158
摘要： Prostasin protein has been found to be a useful marker for determination of the invasiveness of and as a means to treat human carcinomas. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in normal human prostate epithelial cells and the human prostate cancer cell line LNCaP, but not in the highly invasive human prostate cancer cell lines DU-145 and PC-3. Imunohistochemistry studies of human prostate cancer specimens revealed a down-regulation of prostasin in high-grade tumors. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in a non-invasive human breast cancer cell line, MCF-7, while invasive human breast cancer cell lines MDA-MB-231 and MDA-MB-435s were found not to express either the prostasin protein or the mRNA. A non-invasive human breast cancer cell line. MDA-MB-453, was shown to express prostasin mRNA but not prostasin protein. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68% and 42%, respectively. Transfection of MDA-MB-231 and MDA-MB-435s cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 50% for either cell line.
摘要翻译：已经发现前列腺素蛋白是用于测定人类癌的侵袭性和作为手段治疗人类癌症的有用标志物。使用RT-PCR和蛋白质印迹分析，在正常人前列腺上皮细胞和人前列腺癌细胞系LNCaP中发现前列腺素蛋白和mRNA表达，但不存在于高侵袭性人前列腺癌细胞系DU-145和PC-3中。人类前列腺癌标本的免疫组织化学研究显示高级肿瘤中前列腺素的下调。使用RT-PCR和蛋白质印迹分析，在非侵入性人乳腺癌细胞系MCF-7中发现前列腺素蛋白和mRNA表达，而侵袭性人乳腺癌细胞系MDA-MB-231和MDA-MB-435s分别为发现不表达前列腺素蛋白或mRNA。非侵入性人乳腺癌细胞系。 MDA-MB-453显示表达前列腺素mRNA而不是前列腺素蛋白。 DU-145和PC-3细胞用全长人前列腺素cDNA的转染能够恢复前列腺素的表达，体外侵袭能力分别降低了68％和42％。用全长人前列腺素cDNA转染MDA-MB-231和MDA-MB-435s细胞恢复前列腺素的表达，并将任何细胞系的体外侵袭力降低50％。

3. 发明授权

US07666981B1 Inhibitors of prostasin 失效
标题翻译：前列腺素抑制剂
公开(公告)号：US07666981B1
公开(公告)日：2010-02-23
申请号：US10434349
申请日：2003-05-08
申请人： Karl X. Chai , Li-Mei Chen
发明人： Karl X. Chai , Li-Mei Chen
IPC分类号： C07K14/00
CPC分类号： C12N9/6424
摘要： A class of proteins useful as inhibitors of prostasin and method for identifying them are provided. These proteins have the structure wherein the amino acids P1-P4 from the scissile bond are respectively leu-ile-ala-arg and the amino acids at positions P5-P15 are serpin sequences.
摘要翻译：提供了一类可用作前列腺素抑制剂的蛋白质及其鉴定方法。这些蛋白质具有这样的结构，其中来自于剪切键的氨基酸P1-P4分别是亮氨酸，而位置P5-P15的氨基酸是丝氨酸序列。

4. 发明授权

US06864093B1 Method of identifying and treating invasive carcinomas 失效
标题翻译：识别和治疗侵袭性癌的方法
公开(公告)号：US06864093B1
公开(公告)日：2005-03-08
申请号：US10235255
申请日：2002-09-05
申请人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
发明人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
IPC分类号： C12Q1/68 , G01N33/574 , G01N33/48 , A61K49/00 , C07H21/02 , C07H21/04
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158
摘要： Prostasin protein has been found to be a useful marker for determination of the invasiveness of and as a means to treat human carcinomas. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in normal human prostate epithelial cells and the human prostate cancer cell line LNCaP, but not in the highly invasive human prostate cancer cell lines DU-145 and PC-3. Imunohistochemistry studies of human prostate cancer specimens revealed a down-regulation of prostasin in high-grade tumors. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in a non-invasive human breast cancer cell line, MCF-7, while invasive human breast cancer cell lines MDA-MB-231 and MDA-MB-435s were found not to express either the prostasin protein or the mRNA. A non-invasive human breast cancer cell line, MDA-MB-453, was shown to express prostasin mRNA but not prostasin protein. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68% and 42%, respectively. Transfection of MDA-MB-231 and MDA-MB-435s cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 50% for either cell line. The prostasin gene promoter region was found to be hypermethylated at specific sites in invasive cancer cells.
摘要翻译：已经发现前列腺素蛋白是用于测定人类癌的侵袭性和作为手段治疗人类癌症的有用标志物。使用RT-PCR和蛋白质印迹分析，在正常人前列腺上皮细胞和人前列腺癌细胞系LNCaP中发现前列腺素蛋白和mRNA表达，但不存在于高侵袭性人前列腺癌细胞系DU-145和PC-3中。人类前列腺癌标本的免疫组织化学研究显示高级肿瘤中前列腺素的下调。使用RT-PCR和蛋白质印迹分析，在非侵入性人乳腺癌细胞系MCF-7中发现前列腺素蛋白和mRNA表达，而侵袭性人乳腺癌细胞系MDA-MB-231和MDA-MB-435s分别为发现不表达前列腺素蛋白或mRNA。非侵入性人乳腺癌细胞系MDA-MB-453显示表达前列腺素mRNA而不是前列腺素蛋白。 DU-145和PC-3细胞用全长人前列腺素cDNA的转染能够恢复前列腺素的表达，体外侵袭能力分别降低了68％和42％。用全长人前列腺素cDNA转染MDA-MB-231和MDA-MB-435s细胞恢复前列腺素的表达，并将任何细胞系的体外侵袭力降低50％。发现前列腺素基因启动子区域在侵袭性癌细胞的特定部位被高甲基化。

5. 发明授权

US06569684B2 Method of identifying and treating invasive carcinomas 失效
标题翻译：识别和治疗侵袭性癌的方法
公开(公告)号：US06569684B2
公开(公告)日：2003-05-27
申请号：US09755811
申请日：2001-01-05
申请人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
发明人： Karl X. Chai , Li-Mei Chen , Lee Chao , Julie Chao
IPC分类号： G01N3348
CPC分类号： G01N33/574 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158
摘要： Prostasin protein has been found to be a useful marker for determination of the invasiveness of and as a means to treat human carcinomas. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in normal human prostate epithelial cells and the human prostate cancer cell line LNCaP, but not in the highly invasive human prostate cancer cell lines DU-145 and PC-3. Imunohistochemistry studies of human prostate cancer specimens revealed a down-regulation of prostasin in high-grade tumors. Using RT-PCR and western blot analyses, prostasin protein and mRNA expression were found in a non-invasive human breast cancer cell line, MCF-7, while invasive human breast cancer cell lines MDA-MB-231 and MDA-MB-435s were found not to express either the prostasin protein or the mRNA. A non-invasive human breast cancer cell line, MDA-MB-453, was shown to express prostasin mRNA but not prostasin protein. Transfection of DU-145 and PC-3 cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 68% and 42%, respectively. Transfection of MDA-MB-231 and MDA-MB-435s cells with a full-length human prostasin cDNA restored prostasin expression and reduced the in vitro invasiveness by 50% for either cell line.
摘要翻译：已经发现前列腺素蛋白是用于测定人类癌的侵袭性和作为手段治疗人类癌症的有用标志物。使用RT-PCR和蛋白质印迹分析，在正常人前列腺上皮细胞和人前列腺癌细胞系LNCaP中发现前列腺素蛋白和mRNA表达，但不存在于高侵袭性人前列腺癌细胞系DU-145和PC-3中。人类前列腺癌标本的免疫组织化学研究显示高级肿瘤中前列腺素的下调。使用RT-PCR和蛋白质印迹分析，在非侵入性人乳腺癌细胞系MCF-7中发现前列腺素蛋白和mRNA表达，而侵袭性人乳腺癌细胞系MDA-MB-231和MDA-MB-435s分别为发现不表达前列腺素蛋白或mRNA。非侵入性人乳腺癌细胞系MDA-MB-453显示表达前列腺素mRNA而不是前列腺素蛋白。 DU-145和PC-3细胞用全长人前列腺素cDNA的转染能够恢复前列腺素的表达，体外侵袭能力分别降低了68％和42％。用全长人前列腺素cDNA转染MDA-MB-231和MDA-MB-435s细胞恢复前列腺素的表达，并将任何细胞系的体外侵袭力降低50％。

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