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1. 发明授权

US08524448B2 Method and substances for isolating miRNAs 有权
公开(公告)号：US08524448B2
公开(公告)日：2013-09-03
申请号：US13598003
申请日：2012-08-29
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6876 , C12N15/1068 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207 , C12Q2525/191 , C12Q2521/501
摘要： A capture probe suitable for use with a method for isolating miRNAs. A method for isolating an miRNA of interest from a sample comprising the miRNA of interest comprising providing the capture probe. A method for identifying an miRNA of interest.

2. 发明申请

US20120329054A1 METHOD AND SUBSTANCES FOR ISOLATING MIRNAS 有权
标题翻译：用于分离乳晕的方法和物质
公开(公告)号：US20120329054A1
公开(公告)日：2012-12-27
申请号：US13598003
申请日：2012-08-29
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12P19/34 , C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6876 , C12N15/1068 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207 , C12Q2525/191 , C12Q2521/501
摘要： A capture probe suitable for use with a method for isolating miRNAs. A method for isolating an miRNA of interest from a sample comprising the miRNA of interest comprising providing the capture probe. A method for identifying an miRNA of interest.
摘要翻译：适用于分离miRNA的方法的捕获探针。从包含感兴趣的miRNA的样品中分离感兴趣的miRNA的方法，包括提供捕获探针。用于鉴定感兴趣的miRNA的方法。

3. 发明申请

US20090023151A1 Method For The Labeling And Detection Of Small Polynucleotides 审中-公开
标题翻译：小型多核苷酸的标记和检测方法
公开(公告)号：US20090023151A1
公开(公告)日：2009-01-22
申请号：US12202133
申请日：2008-08-29
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6813 , C12Q2525/207 , C12Q2525/161
摘要： Methods and kits for use in isolating, labeling or detecting a small polynucleotide of interest from a sample. The method entails hybridizing the small polynucleotide to a capture probe, lengthening the small polynucleotide by primer extension and/or ligation, and degrading the capture probe to provide a single stranded extension product. The kits include the capture probe and additional reagents for the extension, ligation and/or degradation reactions.
摘要翻译：用于从样品中分离，标记或检测感兴趣的小型多核苷酸的方法和试剂盒。该方法需要将小多核苷酸与捕获探针杂交，通过引物延伸和/或连接来延长小多核苷酸，并降解捕获探针以提供单链延伸产物。试剂盒包括捕获探针和用于延伸，连接和/或降解反应的其他试剂。

4. 发明申请

US20080268428A1 Chromosome 5 Genetic Variants Related to Dyslexia 审中-公开
标题翻译：染色体5遗传变异与阅读障碍相关
公开(公告)号：US20080268428A1
公开(公告)日：2008-10-30
申请号：US10578700
申请日：2004-11-12
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/53 , A61B5/00
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： An isolated polynucleotide or genetic material from human Chromosome 5 that indicates the presence of dyslexia or a predisposition to develop dyslexia in the individual from whom which the sample was obtained. A method of diagnosing dyslexia or a predisposition to develop dyslexia.
摘要翻译：来自人类染色体5的分离的多核苷酸或遗传物质，其指示在获得样品的个体中存在诵读困难或倾向于发展诵读困难的倾向。诊断诵读困难或倾向发展为诵读困难的方法。

5. 发明申请

US20080160511A1 Method and Substances For Isolating miRNAs 有权
标题翻译：分离miRNA的方法和物质
公开(公告)号：US20080160511A1
公开(公告)日：2008-07-03
申请号：US10593383
申请日：2006-08-18
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C07H1/00 , C07H21/00 , C12N13/00
CPC分类号： C12Q1/6876 , C12N15/1068 , C12Q1/6816 , C12Q2600/158 , C12Q2600/178 , C12Q2525/207 , C12Q2525/191 , C12Q2521/501
摘要： A capture probe suitable for use with a method for isolating miRNAs. A method for isolating an miRNA of interest from a sample comprising the miRNA of interest comprising providing the capture probe. A method for identifying an miRNA of interest.
摘要翻译：适用于分离miRNA的方法的捕获探针。从包含感兴趣的miRNA的样品中分离感兴趣的miRNA的方法，包括提供捕获探针。用于鉴定感兴趣的miRNA的方法。

6. 发明申请

US20130116154A1 METHOD AND SUBSTANCES FOR ISOLATION AND DETECTION OF SMALL POLYNUCLEOTIDES 审中-公开
标题翻译：用于分离和检测小聚合物的方法和物质
公开(公告)号：US20130116154A1
公开(公告)日：2013-05-09
申请号：US13678368
申请日：2012-11-15
申请人： Elliott P. DAWSON , Kristie E. WOMBLE
发明人： Elliott P. DAWSON , Kristie E. WOMBLE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/682 , C12Q2525/207 , C12Q2525/161 , C12Q2525/143
摘要： A capture probe suitable for use with methods for isolating, labeling or detecting small polynucleotides. A method for isolating a small polynucleotide of interest from a sample comprising hybridizing the small polynucleotide to the capture probe and lengthening the small polynucleotide by primer extension or ligation. A method for detecting a small polynucleotide of interest following isolation by amplification of the primer extension products and/or hybridization and subsequent cleavage of dual labeled detector probes.
摘要翻译：适用于分离，标记或检测小多核苷酸的方法的捕获探针。一种用于从样品中分离感兴趣的小型多核苷酸的方法，包括将小多核苷酸与捕获探针杂交并通过引物延伸或连接来延长小多核苷酸。通过扩增引物延伸产物和/或双重标记的检测器探针的杂交和随后的切割来分离后检测感兴趣的小多核苷酸的方法。

7. 发明授权

US07858320B2 Method, substances and kits for labeling population of RNA and other substances containing vicinal diols 有权
标题翻译：用于标记含有连位二醇的RNA和其他物质群体的方法，物质和试剂盒
公开(公告)号：US07858320B2
公开(公告)日：2010-12-28
申请号：US12738978
申请日：2008-10-31
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/00 , C07H19/04 , B01L3/00 , C12Q1/70
CPC分类号： G01N33/532 , Y10T436/143333 , Y10T436/147777 , Y10T436/203332 , Y10T436/25
摘要： A method for labeling biological molecules and synthetic molecules that contain a geminal diol, such as RNA, carbohydrates or glycoproteins, using a periodate sequestering agent is provided. Compositions and kits for use in the labeling method are also provided.
摘要翻译：提供了使用高碘酸盐螯合剂来标记含有偕二醇（例如RNA，碳水化合物或糖蛋白）的生物分子和合成分子的方法。还提供了用于标记方法的组合物和试剂盒。

8. 发明申请

US20090203017A1 Use of Nucleic Acid Probes to Detect Nucleotide Sequences of Interest in a Sample 审中-公开
标题翻译：使用核酸探针检测样品中感兴趣的核苷酸序列
公开(公告)号：US20090203017A1
公开(公告)日：2009-08-13
申请号：US12359263
申请日：2009-01-23
申请人： ELLIOTT P. DAWSON , STEVEN J. SIMMONS , LORI J. RAY-COX , JENNIFER M. BAKER , KRISTIE E. WOMBLE , JUDITH MADDEN , SUBRAMANI SELLAPPAN , ANDREW HEARN , SAL SEMINARA
发明人： ELLIOTT P. DAWSON , STEVEN J. SIMMONS , LORI J. RAY-COX , JENNIFER M. BAKER , KRISTIE E. WOMBLE , JUDITH MADDEN , SUBRAMANI SELLAPPAN , ANDREW HEARN , SAL SEMINARA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2563/107 , C12Q2521/301
摘要： The invention relates to methods for the determination and detection of nucleic acids sequences in a sample. The nucleic acid may be RNA or DNA or both. The invention also relates to methods for the determination of the presence and species of various microorganisms in a sample. We have also identified a set of oligonucleotide nucleic acid sequences within the rRNAs of Gram-negative organisms that facilitates both the broad identification of Gram-negative organisms as a class when used as a pool, or in combination, for example in a hybridization assay. This set of oligonucleotides may detect sequences that are indicative of the presence of organisms of the broad class of Gram-negative organisms while exhibiting little or no false identification of Gram-positive organisms, and fungi, or other microorganisms. The assay includes concurrent incubation with at least one nucleotide sequence of interest, at least one nucleic acid probe, a fluorosurfactant, and a nuclease. The assay may further be employed to detect the presence of bacteria, fungi, or other microorganisms by use of additional specific probes, or to detect and/or identify target nucleic acid sequences in a sample. Further, the invention also relates to methods of reducing non-specific binding and facilitating complex formation in a binding assay. The binding assay may be, but is not limited to, a nucleic acid hybridization assay or an immunoassay. The invention also relates to methods of detection that employ at least one target of interest, which may be a nucleotide sequence, at least one probe, which may be a nucleic acid probe and a nuclease.
摘要翻译：本发明涉及用于测定和检测样品中核酸序列的方法。核酸可以是RNA或DNA或两者。本发明还涉及用于确定样品中各种微生物的存在和种类的方法。我们还在革兰氏阴性生物体的rRNAs中鉴定了一组寡核苷酸核酸序列，当用作池或组合使用时，例如在杂交测定中，可以广泛鉴定革兰氏阴性生物体。这组寡核苷酸可以检测指示广泛类型的革兰氏阴性生物体的生物体的存在的序列，同时表现出对革兰氏阳性生物体，真菌或其它微生物的很少或没有虚假的鉴定。该测定包括与至少一个目的核苷酸序列，至少一个核酸探针，含氟表面活性剂和核酸酶的同时孵育。该测定法还可用于通过使用另外的特异性探针来检测细菌，真菌或其它微生物的存在，或检测和/或鉴定样品中的靶核酸序列。此外，本发明还涉及减少非特异性结合并促进结合测定中复合物形成的方法。结合测定可以是但不限于核酸杂交测定或免疫测定。本发明还涉及使用至少一种感兴趣靶标（其可以是核苷酸序列）的至少一种探针，其可以是核酸探针和核酸酶的检测方法。

9. 发明申请

US20080280290A1 Method and Device for the Collection and Isolation of Nucleic Acid 审中-公开
标题翻译：用于收集和分离核酸的方法和装置
公开(公告)号：US20080280290A1
公开(公告)日：2008-11-13
申请号：US10599248
申请日：2006-08-18
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： B01L3/5085 , B01L3/50857 , B01L2300/069 , B01L2300/0819
摘要： A device for collecting and preserving nucleic acids in a sample, the device comprising: a) a support; b) one or more than one sample zone in the support for loading the sample onto the device; and c) a composition comprising i) one or more than one absorbent, and ii) one or more than one stabilizer; where the one or more than one sample zone on the support comprises a recess or space within the support extending from the top surface toward, but not through, the bottom surface, or comprises a space within the support and the composition is retained within the sample zone. A method for collecting and preserving nucleic acids in a sample, the method comprising a) providing a device for collecting and preserving nucleic acids in a sample according to the present invention; b) providing a sample potentially comprising one or more than one nucleic acid; and c) applying part or all of the sample to one or more than one of the sample zones on the device. A method of detecting and quantifying nucleic acids in a sample, the method comprising a) collecting and preserving nucleic acids in the sample according to a method of the present invention; b) removing the absorbent with sample from the sample zones of the device; and c) detecting, or detecting and quantifying the nucleic acids.
摘要翻译：一种用于收集和保存样品中的核酸的装置，所述装置包括：a）支撑体; b）用于将样品装载到装置上的载体中的一个或多个样品区; 和c）包含i）一种或多于一种吸收剂的组合物，和ii）一种或多种稳定剂; 其中支撑体上的一个或多于一个样品区域包括在支撑件内的凹部或空间，其从顶表面向底表面延伸但不穿过底表面，或包括支撑体内的空间，并且组合物保留在样品内区。一种用于收集和保存样品中核酸的方法，所述方法包括：a）提供根据本发明的样品中收集和保存核酸的装置; b）提供潜在地包含一种或多于一种核酸的样品; 以及c）将部分或全部样品施加到装置上的一个或多于一个样品区域中。一种检测和定量样品中核酸的方法，所述方法包括：a）根据本发明的方法收集和保存样品中的核酸; b）从设备的样品区域中取出样品的吸收剂; 和c）检测或检测和定量核酸。

10. 发明授权

US08329394B2 Methods and substances for isolation and detection of small polynucleotides 有权
标题翻译：用于分离和检测小多核苷酸的方法和物质
公开(公告)号：US08329394B2
公开(公告)日：2012-12-11
申请号：US11958180
申请日：2007-12-17
申请人： Ellott P. Dawson , Kristie E. Womble
发明人： Ellott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/682 , C12Q2525/207 , C12Q2525/161 , C12Q2525/143
摘要： A capture probe suitable for use with methods for isolating, labeling or detecting small polynucleotides. A method for isolating a small polynucleotide of interest from a sample comprising hybridizing the small polynucleotide to the capture probe and lengthening the small polynucleotide by primer extension or ligation. A method for detecting a small polynucleotide of interest following isolation by amplification of the primer extension products and/or hybridization and subsequent cleavage of dual labeled detector probes.
摘要翻译：适用于分离，标记或检测小多核苷酸的方法的捕获探针。一种用于从样品中分离感兴趣的小型多核苷酸的方法，包括将小多核苷酸与捕获探针杂交并通过引物延伸或连接来延长小多核苷酸。通过扩增引物延伸产物和/或双重标记的检测器探针的杂交和随后的切割来分离后检测感兴趣的小多核苷酸的方法。

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