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1. 发明申请

US20130116154A1 METHOD AND SUBSTANCES FOR ISOLATION AND DETECTION OF SMALL POLYNUCLEOTIDES 审中-公开
标题翻译：用于分离和检测小聚合物的方法和物质
公开(公告)号：US20130116154A1
公开(公告)日：2013-05-09
申请号：US13678368
申请日：2012-11-15
申请人： Elliott P. DAWSON , Kristie E. WOMBLE
发明人： Elliott P. DAWSON , Kristie E. WOMBLE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/682 , C12Q2525/207 , C12Q2525/161 , C12Q2525/143
摘要： A capture probe suitable for use with methods for isolating, labeling or detecting small polynucleotides. A method for isolating a small polynucleotide of interest from a sample comprising hybridizing the small polynucleotide to the capture probe and lengthening the small polynucleotide by primer extension or ligation. A method for detecting a small polynucleotide of interest following isolation by amplification of the primer extension products and/or hybridization and subsequent cleavage of dual labeled detector probes.
摘要翻译：适用于分离，标记或检测小多核苷酸的方法的捕获探针。一种用于从样品中分离感兴趣的小型多核苷酸的方法，包括将小多核苷酸与捕获探针杂交并通过引物延伸或连接来延长小多核苷酸。通过扩增引物延伸产物和/或双重标记的检测器探针的杂交和随后的切割来分离后检测感兴趣的小多核苷酸的方法。

2. 发明申请

US20120031845A1 Devices and Methods for the Purification, Isolation, Desalting or Buffer/Solvent Exchange of Substances 有权
标题翻译：纯化，分离，脱盐或缓冲/溶剂交换物质的装置和方法
公开(公告)号：US20120031845A1
公开(公告)日：2012-02-09
申请号：US13274185
申请日：2011-10-14
申请人： Elliott P. Dawson
发明人： Elliott P. Dawson
IPC分类号： B01D15/08
CPC分类号： G01N30/6091 , B01D15/22 , B01J20/267 , B01J20/283 , B01J20/285 , B01J20/291 , B01J2220/58 , B01L3/5021 , B01L2300/042 , B01L2300/0681 , B01L2300/0832 , G01N30/6052 , G01N2030/381 , Y10T436/255
摘要： A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
摘要翻译：旋转柱装置，其包含在离心期间保持其形状的刚性多孔过滤器，使用该装置的色谱方法将期望的物质（例如生物分子）与混合物中的其它物质分离，以及包含装置中的一个或多个更多用于该方法的试剂。

3. 发明授权

US07858320B2 Method, substances and kits for labeling population of RNA and other substances containing vicinal diols 有权
标题翻译：用于标记含有连位二醇的RNA和其他物质群体的方法，物质和试剂盒
公开(公告)号：US07858320B2
公开(公告)日：2010-12-28
申请号：US12738978
申请日：2008-10-31
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/00 , C07H19/04 , B01L3/00 , C12Q1/70
CPC分类号： G01N33/532 , Y10T436/143333 , Y10T436/147777 , Y10T436/203332 , Y10T436/25
摘要： A method for labeling biological molecules and synthetic molecules that contain a geminal diol, such as RNA, carbohydrates or glycoproteins, using a periodate sequestering agent is provided. Compositions and kits for use in the labeling method are also provided.
摘要翻译：提供了使用高碘酸盐螯合剂来标记含有偕二醇（例如RNA，碳水化合物或糖蛋白）的生物分子和合成分子的方法。还提供了用于标记方法的组合物和试剂盒。

4. 发明申请

US20090203017A1 Use of Nucleic Acid Probes to Detect Nucleotide Sequences of Interest in a Sample 审中-公开
标题翻译：使用核酸探针检测样品中感兴趣的核苷酸序列
公开(公告)号：US20090203017A1
公开(公告)日：2009-08-13
申请号：US12359263
申请日：2009-01-23
申请人： ELLIOTT P. DAWSON , STEVEN J. SIMMONS , LORI J. RAY-COX , JENNIFER M. BAKER , KRISTIE E. WOMBLE , JUDITH MADDEN , SUBRAMANI SELLAPPAN , ANDREW HEARN , SAL SEMINARA
发明人： ELLIOTT P. DAWSON , STEVEN J. SIMMONS , LORI J. RAY-COX , JENNIFER M. BAKER , KRISTIE E. WOMBLE , JUDITH MADDEN , SUBRAMANI SELLAPPAN , ANDREW HEARN , SAL SEMINARA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2563/107 , C12Q2521/301
摘要： The invention relates to methods for the determination and detection of nucleic acids sequences in a sample. The nucleic acid may be RNA or DNA or both. The invention also relates to methods for the determination of the presence and species of various microorganisms in a sample. We have also identified a set of oligonucleotide nucleic acid sequences within the rRNAs of Gram-negative organisms that facilitates both the broad identification of Gram-negative organisms as a class when used as a pool, or in combination, for example in a hybridization assay. This set of oligonucleotides may detect sequences that are indicative of the presence of organisms of the broad class of Gram-negative organisms while exhibiting little or no false identification of Gram-positive organisms, and fungi, or other microorganisms. The assay includes concurrent incubation with at least one nucleotide sequence of interest, at least one nucleic acid probe, a fluorosurfactant, and a nuclease. The assay may further be employed to detect the presence of bacteria, fungi, or other microorganisms by use of additional specific probes, or to detect and/or identify target nucleic acid sequences in a sample. Further, the invention also relates to methods of reducing non-specific binding and facilitating complex formation in a binding assay. The binding assay may be, but is not limited to, a nucleic acid hybridization assay or an immunoassay. The invention also relates to methods of detection that employ at least one target of interest, which may be a nucleotide sequence, at least one probe, which may be a nucleic acid probe and a nuclease.
摘要翻译：本发明涉及用于测定和检测样品中核酸序列的方法。核酸可以是RNA或DNA或两者。本发明还涉及用于确定样品中各种微生物的存在和种类的方法。我们还在革兰氏阴性生物体的rRNAs中鉴定了一组寡核苷酸核酸序列，当用作池或组合使用时，例如在杂交测定中，可以广泛鉴定革兰氏阴性生物体。这组寡核苷酸可以检测指示广泛类型的革兰氏阴性生物体的生物体的存在的序列，同时表现出对革兰氏阳性生物体，真菌或其它微生物的很少或没有虚假的鉴定。该测定包括与至少一个目的核苷酸序列，至少一个核酸探针，含氟表面活性剂和核酸酶的同时孵育。该测定法还可用于通过使用另外的特异性探针来检测细菌，真菌或其它微生物的存在，或检测和/或鉴定样品中的靶核酸序列。此外，本发明还涉及减少非特异性结合并促进结合测定中复合物形成的方法。结合测定可以是但不限于核酸杂交测定或免疫测定。本发明还涉及使用至少一种感兴趣靶标（其可以是核苷酸序列）的至少一种探针，其可以是核酸探针和核酸酶的检测方法。

5. 发明申请

US20080280290A1 Method and Device for the Collection and Isolation of Nucleic Acid 审中-公开
标题翻译：用于收集和分离核酸的方法和装置
公开(公告)号：US20080280290A1
公开(公告)日：2008-11-13
申请号：US10599248
申请日：2006-08-18
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： B01L3/5085 , B01L3/50857 , B01L2300/069 , B01L2300/0819
摘要： A device for collecting and preserving nucleic acids in a sample, the device comprising: a) a support; b) one or more than one sample zone in the support for loading the sample onto the device; and c) a composition comprising i) one or more than one absorbent, and ii) one or more than one stabilizer; where the one or more than one sample zone on the support comprises a recess or space within the support extending from the top surface toward, but not through, the bottom surface, or comprises a space within the support and the composition is retained within the sample zone. A method for collecting and preserving nucleic acids in a sample, the method comprising a) providing a device for collecting and preserving nucleic acids in a sample according to the present invention; b) providing a sample potentially comprising one or more than one nucleic acid; and c) applying part or all of the sample to one or more than one of the sample zones on the device. A method of detecting and quantifying nucleic acids in a sample, the method comprising a) collecting and preserving nucleic acids in the sample according to a method of the present invention; b) removing the absorbent with sample from the sample zones of the device; and c) detecting, or detecting and quantifying the nucleic acids.
摘要翻译：一种用于收集和保存样品中的核酸的装置，所述装置包括：a）支撑体; b）用于将样品装载到装置上的载体中的一个或多个样品区; 和c）包含i）一种或多于一种吸收剂的组合物，和ii）一种或多种稳定剂; 其中支撑体上的一个或多于一个样品区域包括在支撑件内的凹部或空间，其从顶表面向底表面延伸但不穿过底表面，或包括支撑体内的空间，并且组合物保留在样品内区。一种用于收集和保存样品中核酸的方法，所述方法包括：a）提供根据本发明的样品中收集和保存核酸的装置; b）提供潜在地包含一种或多于一种核酸的样品; 以及c）将部分或全部样品施加到装置上的一个或多于一个样品区域中。一种检测和定量样品中核酸的方法，所述方法包括：a）根据本发明的方法收集和保存样品中的核酸; b）从设备的样品区域中取出样品的吸收剂; 和c）检测或检测和定量核酸。

6. 发明授权

US08187476B2 Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances 有权
标题翻译：物质的净化，分离，脱盐或缓冲/溶剂交换的装置和方法
公开(公告)号：US08187476B2
公开(公告)日：2012-05-29
申请号：US13274185
申请日：2011-10-14
申请人： Elliott P. Dawson
发明人： Elliott P. Dawson
IPC分类号： B01L3/02 , B01D21/26 , B01D61/00
CPC分类号： G01N30/6091 , B01D15/22 , B01J20/267 , B01J20/283 , B01J20/285 , B01J20/291 , B01J2220/58 , B01L3/5021 , B01L2300/042 , B01L2300/0681 , B01L2300/0832 , G01N30/6052 , G01N2030/381 , Y10T436/255
摘要： A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
摘要翻译：旋转柱装置，其包含在离心期间保持其形状的刚性多孔过滤器，使用该装置的色谱方法将期望的物质（例如生物分子）与混合物中的其它物质分离，以及包含装置中的一个或多个更多用于该方法的试剂。

7. 发明授权

US08062533B2 Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances 有权
标题翻译：物质的净化，分离，脱盐或缓冲/溶剂交换的装置和方法
公开(公告)号：US08062533B2
公开(公告)日：2011-11-22
申请号：US12674893
申请日：2009-07-31
申请人： Elliott P. Dawson
发明人： Elliott P. Dawson
IPC分类号： B01L3/02 , B01D21/26 , B01D61/00
CPC分类号： G01N30/6091 , B01D15/22 , B01J20/267 , B01J20/283 , B01J20/285 , B01J20/291 , B01J2220/58 , B01L3/5021 , B01L2300/042 , B01L2300/0681 , B01L2300/0832 , G01N30/6052 , G01N2030/381 , Y10T436/255
摘要： A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
摘要翻译：旋转柱装置，其包含在离心期间保持其形状的刚性多孔过滤器，使用该装置的色谱方法将期望的物质（例如生物分子）与混合物中的其它物质分离，以及包含装置中的一个或多个更多用于该方法的试剂。

8. 发明申请

US20090023151A1 Method For The Labeling And Detection Of Small Polynucleotides 审中-公开
标题翻译：小型多核苷酸的标记和检测方法
公开(公告)号：US20090023151A1
公开(公告)日：2009-01-22
申请号：US12202133
申请日：2008-08-29
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6813 , C12Q2525/207 , C12Q2525/161
摘要： Methods and kits for use in isolating, labeling or detecting a small polynucleotide of interest from a sample. The method entails hybridizing the small polynucleotide to a capture probe, lengthening the small polynucleotide by primer extension and/or ligation, and degrading the capture probe to provide a single stranded extension product. The kits include the capture probe and additional reagents for the extension, ligation and/or degradation reactions.
摘要翻译：用于从样品中分离，标记或检测感兴趣的小型多核苷酸的方法和试剂盒。该方法需要将小多核苷酸与捕获探针杂交，通过引物延伸和/或连接来延长小多核苷酸，并降解捕获探针以提供单链延伸产物。试剂盒包括捕获探针和用于延伸，连接和/或降解反应的其他试剂。

9. 发明申请

US20080268428A1 Chromosome 5 Genetic Variants Related to Dyslexia 审中-公开
标题翻译：染色体5遗传变异与阅读障碍相关
公开(公告)号：US20080268428A1
公开(公告)日：2008-10-30
申请号：US10578700
申请日：2004-11-12
申请人： Elliott P. Dawson , Kristie E. Womble
发明人： Elliott P. Dawson , Kristie E. Womble
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/53 , A61B5/00
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： An isolated polynucleotide or genetic material from human Chromosome 5 that indicates the presence of dyslexia or a predisposition to develop dyslexia in the individual from whom which the sample was obtained. A method of diagnosing dyslexia or a predisposition to develop dyslexia.
摘要翻译：来自人类染色体5的分离的多核苷酸或遗传物质，其指示在获得样品的个体中存在诵读困难或倾向于发展诵读困难的倾向。诊断诵读困难或倾向发展为诵读困难的方法。

10. 发明授权

US06322988B1 Method for determining polynucleotide sequence variations 失效
标题翻译：确定多核苷酸序列变异的方法
公开(公告)号：US06322988B1
公开(公告)日：2001-11-27
申请号：US09719130
申请日：2000-12-08
申请人： Elliott P. Dawson , John A. Phillips, III
发明人： Elliott P. Dawson , John A. Phillips, III
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q1/6827 , C12Q2535/125 , C12Q2537/143 , C12Q2563/107
摘要： A method of determining the presence and identity of a variation in a nucleotide sequence between a first polynucleotide and a second polynucleotide comprising, first, providing a sample of the first polynucleotide and selecting a region of the first polynucleotide potentially containing the variation. Then, the selected region is subjected to a template producing amplification reaction to produce a plurality of double stranded polynucleotide templates which include the selected region. Next, a family of labeled, linear polynucleotide fragments is produced from both strands of the template simultaneously by a fragment producing reaction using a set of primers. Then, the location and identity of at least some of the bases in the selected region of the first polynucleotide is determined using the labels present in the fragments. Next, the location and identity of the bases determined is compared with the location and identity of bases from a second polynucleotide, thereby identifying the presence and identity of a variation in a nucleotide sequence between the selected region of the first polynucleotide and a corresponding region of the second polynucleotide.
摘要翻译：确定第一多核苷酸和第二多核苷酸之间的核苷酸序列变体的存在和身份的方法，其包括首先提供第一多核苷酸的样品并选择潜在含有该变体的第一多核苷酸的区域。然后，对所选择的区域进行模板产生扩增反应以产生包括所选区域的多个双链多核苷酸模板。接下来，通过使用一组引物的片段产生反应同时由模板的两条链同时产生一系列标记的线性多核苷酸片段。然后，使用片段中存在的标记确定第一多核苷酸的选定区域中至少一些碱基的位置和身份。接下来，将确定的碱基的位置和身份与来自第二多核苷酸的碱基的位置和身份进行比较，从而鉴定第一多核苷酸的所选区域与第一多核苷酸的选择区域之间的核苷酸序列的变异的存在和身份第二多核苷酸。

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